Debjani Dutta

• PhD
• Professor (Associate) at National Institute of Technology Durgapur

Ultraviolet-induced skin damage is very frequent due to the severe climatic change taking place globally. Treatment of skin damage using bioactive is the sought remedy where the molecule in question is being encapsulated in a synthetic polymeric material. It has been observed that such fabrication leads to a toxic impact on the skin with time. To o...

Essential oils (EOs) obtained from plant resources have potential use in the arena of food packaging and preservation owing to their excellent antioxidant and antimicrobial properties. However, direct uses of essential oils in food industries are limited because of their low solubility in water and loss of bioactivities in unfavorable environmental...

Free radicals generated during the metabolic process are elevated in tumor cells. Natural antioxidants like polyphenols are considered a promising chemotherapeutic to alleviate cancer. Polyphenol containing mangiferin has multifactorial medicinal effects, but the low oral bioavailability restricts its efficacy. This study focuses on the therapeutic...

Natural antioxidants have become vital to minimize macromolecular damage caused by Reactive Oxygen Species (ROS). This study investigated the antioxidant property of β-cryptoxanthin (β-CRX) extracted from Kocuria marina DAGII and its protective effect against macromolecular damages by generating ROS via two models: UV radiation and the Fenton react...

In the food industry, food quality and safety are vital, and in this case, appropriate packaging technology can significantly ensure the quality of food for consumers. Therefore, innovation in food packaging technology is believed to cater to the rising global demand for food quality and safety. The application of CRISPR technology for the ultra-se...

Citrus fruits are extremely popular for their rich nutritional attributes and health benefits, but they are perishable in nature, and growers and suppliers often face financial losses due to rapid postharvest decay in the fruits. In this study, carnauba wax (CW) based sustainable active coatings were developed, characterized and applied for posthar...

Preservation not only reduces the water activity (aw) of food materials but also inhibits microbial growth, enhances shelf life, and reduces packaging and transportation costs. Currently, researchers are focusing on the production of low carbon footprint technologies. However, conventional preservation treatments produce a large volume of environme...

Perishable foods like fruits and vegetables, meat, fish, and dairy products have short shelf-life that causes significant postharvest losses, which poses a major challenge for food supply chains. Biopolymers have been extensively studied as sustainable alternatives to synthetic plastics, and pectin is one such biopolymer that has been used for pack...

Microbial pigment production has become popular these days due to its numerous application in various fields like pharmaceutical, food, and cosmetic industries. Color of an object attributes its quality that attracts our attention. Increasing awareness of toxicity of synthetic colors has surfaced consumers’ preference for natural food color additiv...

Recently vitamin A deficiency disorder (VADD) has become a significant public health issue. Manifestation of this VADD is xerophthalmia, which can only be prevented by additional vitamin A due to their less consumption in regular diet. Globally, approximately 120 million children are suffering from VADD. There is an abrupt upsurge in non-communicab...

Background Mangiferin is a C-glycoside xanthone molecule having a wide range of therapeutic properties. Hence, the present study aims to understand the efficacy of mangiferin against colorectal cancer (CRC) and to elucidate the mechanisms of action of mangiferin on colorectal cancer. Method The molecular mechanism of mangiferin against colorectal...

The bioactive red-colored carotenoid grabs attention due to its antioxidant properties, though it has no provitamin A activity. It has the highest singlet oxygen quenching ability among all carotenoids. Lycopene is responsible for the red to pink colors observed in many fruits and vegetables. The unique structure of lycopene for its ruby red color...

The Publisher regrets that this article is an accidental duplication of an article that has already been published, https://dx.doi.org/10.1016/j.matpr.2022.01.004. The duplicate article has therefore been withdrawn. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal

Obesity is one of the significant risk factors commonly associated with lots of metabolic diseases penetrating in the younger age group population. In this study, we aimed to check the effectiveness of a carotenoid, β-cryptoxanthin, for reducing the adverse effect of obesity. The possible metabolic pathway of β-cryptoxanthin towards lowering obesit...

Beta-cryptoxanthin (β-CRX), a pro vitamin A carotenoid was extracted from Kocuria marina DAGII, a gram positive bacteria isolated from soil. To determine the antioxidant activity of β-CRX against free radicals, DPPH, ABTS, H2O2 and TAC assay were performed. The scavenging results showed antioxidant activity of beta-cryptoxanthin. The scavenging act...

Succinic acid has been extensively used as a precursor for the synthesis of various pharmaceutical- and food-grade chemicals of industrial significance. In general, succinic acid is synthesised through chemical methods such as electroreduction or catalytic hydrogenation of maleic acid or paraffin oxidation. However, these chemical routes suffer fro...

Climate change, environmental security, and sustainability of petrochemical resources along with depletion of fossil fuels are the current immerging issues all over the world. The industrial production of commodity chemicals from agricultural biomass provides a noteworthy contribution to solving the above mentioned environmental problems. The use o...

This study aims for eco-friendly removal of Malachite green (MG) by ‘biosorption’ process. In our study, Kocuria marina DAGII isolated from soil could effectively decolorize MG approximately up to 99% in continuous study. Column study was performed for MG removal at different parameters to know the probable applicability of bacterial cells in indus...

Oxidative stress is considered to be the leading cause of many chronic and degenerative diseases leading to death and disability. Antioxidant therapy could be efficient in preventing oxidative stress-induced damages in cells. In the present study the efficacy of mangiferin-chitosan nanoparticles (MCNs) as a therapeutic agent against the oxidative s...

Mangiferin‐encapsulated β‐lactoglobulin–chitosan (mangiferin/βLG‐CS) nanoparticles were fortified in milk to obtain a low glycemic index (GI) food product with high antioxidant capacity. The present study evaluated the bioaccessibility and bioavailability of mangiferin in milk fortified with nanoparticles. Fortification reduced the GI of milk by 34...

Mangiferin, a bioactive compound with numerous therapeutic properties was extracted from Curcuma amada. To solve the bioavailability issues of mangiferin, it was encapsulated in β-lactoglobulin (β-LG), by desolvation method and reduced to a nano size. From the Dynamic Light Scattering (DLS) data, average particle size and zeta potential of the nano...

The dairy industry produces enormous amount of cheese whey containing the major milk nutrients, but this remains unutilized all over the globe. The present study investigates the production of β-cryptoxanthin (β-CRX) by Kocuria marina DAGII using cheese whey as substrate. Response surface methodology (RSM) and an artificial neural network (ANN) app...

Malachite Green (MG) is a highly toxic synthetic dye which is a major component of textile industry. It alone accounts for the two-third of the total production of the wastewater. Biological decolorization of dye is highly effective and a promising method due to its low cost and environmental friendly approach. The present study involves efficient...

In this present study bacterial canthaxanthin (CX) (extracted from Dietzia maris NITD) was formulated with aloe vera (AV) to obtain a stable suspension. Antioxidant activity was determined through different radical scavenging methods. The formulation was then stored at 03 different temperature viz. and for 05 weeks to check the stability of CX. Lea...

A reddish coloured colony was isolated during routine screening of pigment producing microorganism in the Department of Biotechnology, NIT Durgapur. Preliminary evaluation revealed the microbe to be a Gram positive Coccus. The bacterium was maintained on Brain Heart Infusion plates. For analytical purposes, shake flask experiments were conducted by...

Various natural polymers with hydrophilic properties have been used to form hydrogels for encapsulation and delivery of nutrients and drugs in food and pharmaceutical industries. Among them, chitosan (ChiHG) and alginate (AlgHG) based hydrogel have been extensively explored for delivery of several nutraceuticals in recent years. Release of natural...

In the present investigation, growth kinetics of Kocuria marina DAGII during batch production of β-Cryptoxanthin (β-CRX) was studied by considering the effect of glucose and maltose as a single and binary substrate. The importance of mixed substrate over single substrate has been emphasised in the present study. Different mathematical models namely...

Kocuria marina DAGII, a potent β-cryptoxanthin (β-CRX) producer, was previously isolated in our laboratory during routine screening of pigment producing bacteria. In this study, a classical approach of one factor at a time was applied for obtaining the maximum β-CRX yield by Kocuria marina DAGII. Growth kinetics and reducing sugar estimation were a...

Free radicals generation and cellular oxidation during metabolic activities often results serious cellular damage as well as life threatening diseases in human. Over the years scientific exploration has been extensively carried out to find suitable biomolecules to overcome these issues and carotenoids has that potential to confer such significant a...

The present study was focused on isolation, screening and characterization of biosurfactant producing bacteria and the application of microbial surfactant in agricultural field. Thirty seven bacteria were isolated from diesel contaminated soil and were primarily screened for biosurfactant production using hemolytic method. Based on the result, five...

Liquid phase adsorptive removal of Malachite Green (MG) by an emerging Dietzia species has been investigated in the present study. Crucial operating parameters were screened and optimized by Plackeet–Burman (PB) and Central Composite Design (CCD), respectively, in batch study considering decolorizing efficiency as response. Results showed that init...

Natural colorants like microbial pigment are gaining worldwide attention due to their strong colour and nutraceutical properties. The current study focuses on the extraction of pigment produced by a newly identified bacterium followed by characterization of the pigment by TLC and HPLC. The isolate was identified as Microbacterium pumilum. Growth pa...

Carotenoids are promising dietary supplements to human population due to its potential nutraceutical benefits. Their stability in food system is a big concern for food industry. In this study, stability of β‐cryptoxanthin (β‐CRX), a yellow colored pro‐vitamin A xanthophyll, extracted from a previously isolated bacterium Kocuria marina DAGII, was st...

Mangiferin (MgFn) is a naturally occurring bioactive glucosylxanthone having numerous pharmacological activities. A feasible microwave assisted extraction (MAE) technique for extraction of MgFn from Curcuma amada (mango ginger) was developed in our laboratory. To prove the efficacy of MAE technique, it was compared with other methods like maceratio...

A novel carotenoid producing strain of Dietzia maris NIT-D was isolated from soil and cultured in shake flasks, employing sugarcane bagasse hydrolysate (SCBH) as the carbon source, under optimized conditions. The pigment was identified as trans-canthaxanthin. The purified pigment extracts were submitted to temperature and pH stability analysis and...

A newly isolated yeast strain was identified and explored for characteristic growth and pigment production in batch cultures. Based on nucleotide homology and phylogenetic analysis, the strain was identified as Sporidiobolus pararoseus DAGIII (Accession ID-KF724150). Pigment production was carried out using 1% (v/v) of an inoculum at 25ºC and 120 r...

Dietzia maris NIT-D, a producer of canthaxanthin, is isolated during routine screening of pigment producing bacteria. The effects of process parameters, namely temperature, pH, shaker speed, percentage inoculum, medium volume, and concentration of glucose on the canthaxanthin production are studied by using response surface design methodology. The...

Xenorhabdus indica KB-3, a well-known protease producer, was isolated from its entomopathogenic nematode symbiont Steinernema thermophilum. Since medium constituents are critical to the protease production, the chemical components of the selected medium (soya casein digest broth) were optimized by rotatable central composite design (RCCD) using res...

Dietzia maris NIT-D, a producer of canthaxanthin, is isolated during routine screening of pigment producing bacteria. The effects of process parameters, namely temperature, pH, shaker speed, percentage inoculum, medium volume, and concentration of glucose on the canthaxanthin production are studied by using response surface design methodology. The...

Dimethyl phthalate (DMP) a common environmental pollutant is well known for its endocrine disrupting activities. Wide spread use of the plastics and pesticides in agriculture have resulted in DMP pollution in soils. An endosymbiotic bacteria Xenorhabdus indica isolated from entomopathogenic nematode Steinernema thermophilum was investigated for bio...

Proteases produced by Xenorhabdus are known to play a significant role in virulence leading to insect mortality. Present study was undertaken to purify and characterize protease from Xenorhabdus indica; an endosymbiant of nematode Steinernema thermophilum and deciphering its role in insect mortality and its efficacy to control Helicoverpa armigera....

Mangiferin, a bioactive compound having potent nutraceutical, strong antioxidant and pharmacological significance has been extracted using microwave-assisted extraction (MAE) technique from Curcuma amada, commonly known as mango ginger. The extraction solvent ethanol is eco-friendly, nontoxic and reduces the risk of environmental hazards. The influ...

An extracellular protease was purified and characterized from culture supernatant of Xenorhabdus indica, bacterial symbionts of soil-living entomopathogenic nematodes (EPNs), Steinernema thermophilum using ammonium sulphate precipitation and ion exchange chromatography. The enzyme was 5 fold purified with 54% yield recovery. This was further charac...

Dietzia maris NIT-D, a canthaxanthin producer, was isolated during routine screening of pigment-producing bacteria. Response surface methodology was applied for statistical designing of process parameters for biomass and canthaxanthin production. The effects of four process parameters (considered as independent variables), namely temperature (10–30...

Mangiferin present in Curcuma amada was extracted with the help of microwave assisted extraction (MAE). The extraction solvent used was ethanol, which is eco-friendly and reduced the risk of environmental hazard. The mangiferin content was found to increase until 500 W, but decreased as the microwave power was increased further. A similar threshold...

Adsorption of ginger (Zingiber officinale) polyphenol on an anion exchange resin, Amberlite IR-400 was studied for purification of polyphenols from the crude extract. The work was carried out to understand how the processing parameters can be manipulated to optimize the purification of ginger phenolics for its further use in nutraceuticals and func...

π π π electrons are delocalised over the entire length of the polyene chain is responsible for the molecular shape, chemical and physical reactivity and the antioxidant properties of carotenoids. It is sensitive to heat, light and oxygen. Enzymatic and non-enzymatic oxidation is the major cause of carotenoid destruction during processing and storag...

Optimization of enzyme assay conditions for alkaline protease from Bacillus licheniformis NCIM-2042 was carried out by a statistical approach. Four key determinants such as pH, temperature, buffer concentration, and incubation time were optimized by response surface methodology using rotatable central composite design. Maximum enzyme activity was f...

Dietzia maris was isolated from our laboratory and was grown in Yeast Extract-Peptone-Glucose medium at 37 °C and 180 rpm. The pH of the medium was varied between 1 and 14 and inoculum percentage between 0.5 % and 10 %. Considerable growth and pigmentation was observed at pH between 5 and 12 and at all inoculum percentage, with the maximum yield at...

Polyphenols are secondary metabolites of plants which have antioxidant, antimicrobial as well as antiaging properties that can be beneficial for the humans. However, these plant-derived functional compounds are stable over a certain range of temperature and undergo rapid denaturation beyond that. This thermal range is crucial in the treatment of th...

Astaxanthin is an important natural pigment, a diketo carotenoid that besides being a food ingredient has importance as a nutraceutical. Astaxanthin is a fat-soluble nutrient with a molecular weight of 596.8Da (Dalton) and a molecular formula of C40H52O4. It is water insoluble and lipophilic. Organisms that produce astaxanthin include the basidiomy...

The swelling kinetics of bean seeds have been modeled according to General Maxwell Stefan (GMS) model to allow for the integration of the swelling behavior on moisture absorption. The set of differential equations are solved using Linear Driving Force (LDF) approach and Homogenous Driving Force (HDF) approach. The simulation results are compared wi...

Biochemical reactions are the basis of life, allowing processes move with a time scale. Enzymes are proteins that catalyze the biochemical reactions. These enzyme‐catalyzed reactions are controlled in a number of ways. Among them, inhibitors play a major role. A simulation model helps to monitor the parameters of the reactions at each and every tim...

In this study, selection of suitable carbon, nitrogen and sulphate sources were carried out by one-variable-at-time approach for the production of alkaline protease enzyme by Bacillus licheniformis NCIM-2042. Maximum levels of alkaline protease were found in culture media supplemented with magnesium sulphate, starch and soybean meal as a good sulph...

In this study, selection of suitable carbon, nitrogen and sulphate sources were carried out by one-variable-at-time approach for the production of alkaline protease enzyme by Bacillus licheniformis NCIM-2042. Maximum levels of alkaline protease were found in culture media supplemented with magnesium sulphate, starch and soybean meal as a good sulph...

In this study, selection of suitable carbon, nitrogen and sulphate sources were carried out by one-variable-at-time approach for the production of alkaline protease enzyme by Bacillus licheniformis NCIM-2042. Maximum levels of alkaline protease were found in culture media supplemented with magnesium sulphate, starch and soybean meal as a good sulph...

Various synthetic coloring agents have the potential of carcinogenicity and/or teratogenicity. Natural colors, extracted from fruits, vegetables, seed roots and microorganisms and often called biocolors due to their biological origin have proved to be safe and edible coloring agents. An unidentified, yellow pigment producing bacterium was isolated...

The total carotenoid content and textural properties of pumpkin pieces thermally treated at 55 degrees C, 65 degrees C, 75 degrees C, 85 degrees C and 95 degrees C for different blanch times (3 min, 5 min, and 10 min) and subsequently kept for storage at 0 degrees C, -18 degrees C and -40 degrees C for a period of 80 days were measured. The total c...

Pumpkin pieces were thermally treated at a temperature from SO to 100° for blanching with time intervals of 3, 5, 10, 15 and 20 min. and the β-carotene content, texture and colour (L, a, b) degradation were studied, β-carotene content decreased at all temperatures but the decrease was more at 100° than at 50° and it followed zero order kinetics. Fi...

The degradation kinetics of both the beta-carotene and visual color of pumpkin puree (blanched for 2min in 1% NaCl solution) were determined at a temperature range of 60–100°C for a time period varying between 0 and 2h. An increase in the beta-carotene content was observed when the pumpkin puree was blanched and thermally treated at 60°C. Using the...

Immature pumpkins were stored in dark at 20° and in a freezer at 4, -18 and -40° and changes in β-carotene content and extent of firmness were measured for 10 weeks, β-carotene increased on maturation after which, it decreased in all temperatures. Freezing deteriorated the texture but pumpkin kept in dark and at 4° had firmer texture. Most of the s...

Papaya fruit was treated with citric acid solutions of different concentrations and sodium hydroxide solutions of different molarity (expressed as pH) and stored at 0°, -18° and -40° upto 40 days to study its beta-carotene retention. The beta-carotene content initially increased when the processed papaya fruits were stored for 10 days and afterward...

Carrots were blanched and stored in a freezer to study the retention of β-carotene. Retention was found to be better in 3 min blanched samples than in 5 min ones. There was an increase in β-carotene content in carrots after 5 days at all storage temperatures: 0, -8, -14, and -18°C. Decrease was found to be insignificant compared to the initial valu...

Xenorhabdus sp. SGchamp, mutualistically associated with insect-pathogenic nematodes of the families Steinernematidae are known to produce two colony forms, designated as primary and secondary phase. The primary form is better in its ability to provide nutrients for the nematodes in insects as well as for entomopathogenicity of nematode. The mechan...