Debabrata Dastidar

Guru Nanak institute of pharmaceutical science & technology · Department of Pharmacy

Gram-positive and Gram-negative bacteria often develop biofilm through different mechanisms in promoting pathogenicity. Hence, the antibiofilm molecule needs to be examined separately on both organisms to manage the biofilm threat. Since the antibiofilm activity of piperine against Staphylococcus aureus was already reported; here, we aimed to exami...

Six methyl pheophorbide-a derivatives were prepared by linking tryptamine side chain at C-131, C-152 and C-173 positions of positions methyl and pheophorbide-a. Prepared conjugates were characterized and evaluated for their photocytotoxicity against A549 cells. The conjugate 6a with strong absorption at 413 nm (Soret band), 663-671 nm (Q bands) and...

COVID-19 pandemic is the biggest global crisis. The frequent mutations in coronavirus to generate new mutants is of major concern. The pathophysiology of SARS-CoV-2 infection has been well studied to find out suitable molecular targets and candidate drugs for effective treatment. FDA-recommended etiotropic therapies are currently followed along wit...

Cancer is caused due to the unregulated proliferation of cells. Understanding the differences between a cancer cell and a normal cell; a normal tissue and a tumor microenvironment is the key to developing an efficient targeted drug delivery system for cancer therapy. With recent developments in cancer cell biology, materials science, and nanotechno...

Background Copper nanoparticle (CuNP) has well-established antimicrobial activity. Instability in aqueous medium due to aggregation into larger particles, conversion into metal ions, and oxidation into metal oxides are the major limitations of its practical use against bacterial infections. Objective Development of CuNP Conjugated Chitosan Micropa...

Tryptophan, a naturally occurring aromatic amino acid, is a well-reported versatile molecule in literature. Its importance as an antibiofilm agent against several biofilm-forming organisms has been studied comprehensively. In this study, the antibiofilm effect of tryptophan has been presented against strong biofilm-forming organisms such as Staphyl...

Paclitaxel is the best-selling anticancer drug in history. US-FDA has approved this microtubule depolymerizing agent for the treatment of different types of solid tumors including lung, ovarian, breast, head, and neck cancer, and advanced forms of Kaposi’s sarcoma. But, large molecular structure, insolubility in an aqueous medium, complex pharmacok...

Periodontitis is a serious gum infection associated with inflammation of the periodontium around the teeth. Based on the etiology and pathogenesis, periodontitis has several conventional treatment strategies. Treatment with antibiotics and antimicrobials is the most common therapy. However, development of drug resistance and the manifestation of si...

Recently published clinical data from COVID-19 patients indicated that statin therapy is associated with a better clinical outcome and a significant reduction in the risk of mortality. In this study by computational analysis, we have aimed to predict the possible mechanism of the statin group of drugs by which they can inhibit SARS-CoV-2 pathogenes...

Staphylococcus aureus, a Gram-positive bacterium has been implicated in a plethora of human infections by virtue of its biofilm-forming ability. Inhibition in microbial biofilm formation has been found to be a promising approach towards compromising microbial pathogenesis. In this regard, various natural and synthetic molecules have been explored t...

Respiratory diseases are a major cause of concern throughout the world. Lung cancer, chronic obstructive pulmonary disease (COPD), and asthma deserve special mention among them. According to the WHO, 235 million people suffer from asthma, and above three million people die annually from COPD, which is about 6% of all deaths across the globe. These...

Background Increasing antibiotic-resistance in bacterial strains has boosted the need to find new targets for drug delivery. FtsA, a major bacterial divisome protein can be a potent novel drug-target. Methods and results This study finds, morin (3,5,7,2′,4′-pentahydroxyflavone), a bio-available flavonoid, had anti-bacterial activities against Vibr...

Staphylococcus aureus, a Gram-positive opportunistic microorganism, promotes pathogenicity in the human host through biofilm formation. Microorganisms associated with biofilm often exhibit drug-resistance property that poses a major threat to public healthcare. Thus, the exploration of new therapeutic approaches is the need of the hour to manage bi...

All biological signaling cascade involves electrical and electrochemical impulses. So, the relevant question is “can we find a way to functionally combine biological machine‐like human body to manmade electronic devices to achieve superior advances in biotechnology and medicine?”. Integrative and collaborative research works are going among scienti...

Microbial biofilm indicates a cluster of microorganisms having the capability to display drug resistance property thereby increasing its proficiency in spreading diseases. In the present study, the antibiofilm potential of thymoquinone, a black seed-producing natural molecule was contemplated against the biofilm formation by Pseudomonas aeruginosa....

Bioadhesive films are considered as a novel drug delivery system which provides improved therapy and better patient compliance than conventional dosage forms. The type and concentration of polymer or the blend of polymers used are important factors influencing the properties of bioadhesive films and influencingthe product outcome and performance. B...

Zinc is an essential micronutrient. It has significant roles in innumerable biological processes and enzymatic reactions that occur in human body. Deficiency of zinc causes acrodermatitis enteropathica and excess con- sumption of zinc is responsible for diabetes, cerebral ischemia, epilepsy, neural degeneration, and neurological disorders like Al...

The tumour vasculature plays an important role in tumour growth and metastasis. Tumour angiogenesis provides more oxygen and nutrients to growing tumour cells, is not as tightly regulated as embryonic angiogenesis, and do not follow any hierarchically ordered pattern. The heterogeneity of the vasculature, high interstitial fluid pressure, poor extr...

Pseudomonas aeruginosa is a potent biofilm forming organism causing several diseases on host involving biofilm. Several natural and synthetic molecules have been explored towards inhibiting the biofilm formation of Pseudomonas aeruginosa. In the current report, the role of a natural molecule namely caffeine was examined against the biofilm forming...

Porous microspheres have enormous specific surface area due to the presence of micropores. This makes them suitable for all applications that involves surface adsorption e.g. chromatographic separation of biomolecules, catalytic reactions and drug delivery. The surface property may further be tuned up by functionalization of microsphere surface...

Effect of Apocynin in Non small cell lung carcinoma induced by NNK, a toxic component in cigarette smoke.

Purpose: To determine the efficacy of apocynin(a natural compound) in suppressing the progression of lung carcinoma both in vitro and in vivo, along withthe identification of targets and the underlyingmechanismfor developing a novel therapeutic approach. Methods: We have demonstrated themicrotubule depolymerizing role of apocynin by established pro...

Dexamethasone is clinically very important adrenocortical steroid. But erratic oral absorption leading to irregular bioavailability, is the main problems to its clinical use. In this study a self emulsifying tablet of dexamethasone has been developed. Castor oil, Tween® 80 and Propylene glycol were used as oil vehicle, surfactant and cosurfactant....

Silver nanoparticle (AgNP) has potent anti-microbial activity & chitosan has wound healing activity. L-Glutamic acid is a wound healing promoting agent. So AgNP & L-glutamic acid loaded chitosan hydrogel would be a promising formulation for topical applications like wound healing. But instability of AgNP is the main problem to develop such a topica...

Notch signaling is reported to be deregulated in several malignancies, including breast, and the enzyme γ-secretase plays important role in the activation and nuclear translocation of Notch intracellular domain (NICD). Hence, pharmacological inhibition of γ-secretase might lead to the subsequent inhibition of Notch signaling in cancer cells. In sea...

Theaflavin (TF) and epigallocatechin‐3‐gallate (EGCG) both have been reported previously as microtubule depolymerizing agents that also have anticancer effects on various cancer cell lines and in animal models. Here, we have applied TF and EGCG in combination on HeLa cells to investigate if they can potentiate each other to improve their anticancer...

Porous microspheres have interconnective external and internal pores leading to very low mass density and enormous specific surface area, enabling them to have excellent adsorption capabilities. Due to this uniqueness over traditional microsphere, they find extensive pharmaceutical applications. Porous microspheres are very promising for gastro ret...

Ciprofloxacin hydrochloride is a good candidate drug for gastro retentive drug delivery system (GRDDS). Floating tablet of ciprofloxacin hydrochloride was prepared with wet granulation method using HPMC as matrix material and PVP-K30 as binder. The objective of this study was to find out the effect of granule size on the floating behavior and drug...

Dexamethasone is a glucocorticoid used widely worldwide for immunosuppressive treatment, allergies, bronchiolitis, and croup, among others. However poor aqueous solubility (0.1mg/ml) leads to poor rate of dissolution and hence limits its oral bioavailability. The objective of the present study was to optimize the level of different formulation comp...

Apigenin, a natural flavone, present in many plants sources, induced apoptosis and cell death in lung epithelium cancer (A549) cells with an IC50 value of 93.7 ± 3.7 μM for 48 h treatment. Target identification investigations using A549 cells and also in cell-free system demonstrated that apigenin depolymerized microtubules and inhibited reassembly...