Davor Zeljezic

Davor Zeljezic
Institute for Medical Research and Occupational Health

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175
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Publications

Publications (175)
Article
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222 Uvod Korištenje dentalnih implantata u terapiji bezubosti u stalnom je porastu što je posljedica općeg starenja popula-cije i napretka dentalne tehnologije i terapije. Većina današ-njih implantata proizvodi se od titanija i njegovih legura zbog poželjnih fizičkih i mehaničkih svojstava te dobre biokom-patibilnosti. Biokompatibilnost titanija, k...
Article
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Ochratoxin A (OTA) and citrinin (CTN) are nephrotoxic mycotoxins often found together in grain. The aim of this study was to measure their accumulation in the kidney and liver of adult male Wistar rats, see how it would be affected by combined treatment, and to determine if resveratrol (RSV) would decrease their levels in these organs. The rats rec...
Article
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The aim of this cross-sectional study was to investigate the occurrence of chromo-somal abnormalities through the frequency of micronuclei and other genomic damage markers in patients with chronic generalized periodontitis and without periodontal disease. Micronucleus assay was performed in exfoliated gingival epithelial cells of 35 patients with g...
Article
This paper assessed the potential of trans-placental and -lactational genotoxicity and oxidative stress induction of tembotrione, a naturally derived allelopathic herbicide. Several treatment protocols were applied to measure primary DNA damage by alkaline comet assay in leucocytes and liver. To address the oxidative stress induction, TBARS, ROS, S...
Article
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Aim: The main purpose of this study was to assess the genotoxic and cytotoxic effect of fissure sealants on buccal epithelial cells. Material and methods: The study was conducted on 45 patients (27 girls and 18 boys), seven to 16 years of age (age mean 12.09 ± 2.20). Buccal swabs were collected before (T0), seven (T1), 30 (T2) and 90 days (T3) c...
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Manufacture of lead-containing products has long been associated with various health risks. To get an insight into the related genotoxic risks, we conducted a biomonitoring study in 50 exposed workers and 48 matched controls using a battery of endpoints that sensitively detect the extent of genome instability in peripheral blood lymphocytes. The le...
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The original version of this article unfortunately contained a mistake. The author’s first and last names were interchanged in the published version of this article. The corrected author names are given in this article.
Chapter
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The potential of low doses of the chloro-triazine herbicide terbuthylazine to induce DNA damage and impair activity of glutathione peroxidase (GPx) was evaluated in kidney and parenchymal and non-parenchymal liver cells of adult male rats. In a 28-day study, terbuthylazine was applied daily by oral gavage at doses: 0.004, 0.4 and 2.29 mg/kg bw/day....
Article
Imidacloprid is a neonicotinoid insecticide that acts selectively as an agonist on insect nicotinic acetylcholine receptors. It is used for crop protection worldwide, as well as for non-agricultural uses. Imidacloprid systemic accumulation in food is an important source of imidacloprid exposure. Due to the undisputable need for investigations of im...
Article
Tembotrione is a rather novel pesticide, usually used for post-emergence weed control. Even though its use is rapidly growing, it is not followed by an adequate flow of scientific evidence regarding its toxicity towards non-target organisms. We evaluated the potential of low doses of tembotrione to induce oxidative stress and cytogenetic damage in...
Article
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DNA damage in the liver and kidney cells of adult male Wistar rats was studied using the comet assay after a 28-day oral administration of tembotrione at doses of 0.0007, 0.0013 and 0.7 mg/kg b.w./day [AOEL (acceptable operator exposure level), REL (residual exposure level) and 1000× AOEL]. As a descriptor of DNA damage, tail intensity was used. An...
Article
Objectives: Whereas dental materials came in direct or close contact with oral tissue, it is a great concern about the biocompatibility of those materials. This study was performed to evaluate possible DNA damage to buccal cells exposed to dental materials. Methods: This prospective, longitudinal clinical study was conducted over a three months...
Article
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Objectives This study assessed the frequency of nuclear morphological changes in gingival epithelial cells, as a biomarker for DNA damage, in individuals with periodontitis, before and after implementation of periodontal therapy, and compared the morphology to those with healthy periodontal tissues. Materials and methods Exfoliated gingival cells...
Article
The study aimed to check whether ochratoxin A (OTA) and citrinin (CIT) increase DNA damage in the kidney and liver of male Wistar rats (alkaline comet assay), clarify the oxidative nature of DNA damage (hOGG1-modified comet assay), and verify whether resveratrol (RSV) could ameliorate OTA+CIT-induced genotoxicity. Rats were treated orally with OTA...
Article
Objectives: The present study addresses the effect of fluoride and sodium lauryl sulphate content of toothpaste on oral epithelial cells in vivo conditions. Subjects and method: Forty volunteers were assigned into two experimental groups, each of them applying the different brand of toothpaste. Every group has been using three different types of to...
Poster
Terbuthylazine is a chloro-triazine herbicide that acts primarily as an inhibitor of photosynthesis. It is used mainly in the production of maize but is also applied in the cultivation of various fruits, and vegetables, as well as in forestry. We evaluated the in vivo effects of terbuthylazine following a 28- day oral administration in adult male W...
Poster
We investigated the effect of low-dose exposure to glyphosate on 3 months old male Wistar rats that received p.o. for 28-day doses of 0.1 mg kg-1 b.w. of the acceptable operator exposure level-(AOEL), 0.5 mg kg-1 b.w. consumers acceptable daily intake (ADI), 1.75 mg kg-1 b.w. of the chronic population-adjusted dose (cPAD), 10 mg kg-1 b.w. (100xAOEL...
Article
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Nucleoside analogues represent an important class of compounds of wide application range in antiviral and antitumor therapy. A regular procedure in early phases of new drugs development includes assessment of cytotoxicity and genotoxicity (mutagenicity). Here we report the synthesis of two novel C-5 hydroxypropyl pyrimidine nucleosides bearing side...
Article
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Objective: The aim of this study was to evaluate cytotoxic potencies of two light cured composite materials after heating on different temperatures and cured directly and through CAD/CAM overlay. Materials and methods: Composite materials (microfilled-hybrid Gradia Direct Posterior and Beautifil II) were heated in a Calset warming unit at three...
Article
Purpose This is a cross‐sectional study to assess the incidence of micronuclei and other nuclear morphological changes in buccal epithelial cells of dental technicians. Materials and Methods The study was conducted on 45 dental technicians vs. 2 control groups: 50 dentists and 50 dental assistants. DNA damage was analyzed in exfoliated buccal muco...
Article
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In this study, the influence of anthropogenic pollution on the aquatic environment of Plitvice Lakes National Park (PLNP) was investigated during 2011–2012 using a combination of chemical and cytogenetic analyses. Four groups of major contaminants [(volatile organic compounds: benzene, toluene, ethylbenzene, and xylenes (BTEX); persistent organochl...
Article
Chlorpyrifos, imidacloprid, and α‐cypermethrin are some of the most widely used insecticides in contemporary agriculture. However, their low‐dose, nontarget genotoxic effects have not been extensively assayed. As one of the most relevant cancer biomarkers, we aimed to assess the aneuploidy due to chromosome missegregation during mitosis. To aim it...
Article
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Objectives: The aim was to compare cytotoxicity/genotoxicity of pre-heated composites polymerized through CAD/CAM overlays on isolated human peripheral blood lymphocytes. Material and methods: A microhybrid (Z100, 3M ESPE) and nanofilled composite (Filtek Supreme Ultra, 3M ESPE) were heated in a heating unit (Calset, AdDent Inc.) at different te...
Poster
Tembotrione, a triketone herbicide used for post-emergence weed control in corn, as a potential endocrine disrupting chemical can disrupt reproductive and sexual development by affecting sex hormones. This could be especially detrimental during early development with adverse effects until adulthood. We evaluated the effects of tembotrione exposure...
Article
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In this 28 day-study, we evaluated the effects of herbicide glyphosate administered by gavage to Wistar rats at daily doses equivalent to 0.1 of the acceptable operator exposure level (AOEL), 0.5 of the consumer acceptable daily intake (ADI), 1.75 (corresponding to the chronic population-adjusted dose, cPAD), and 10 mg kg-1 body weight (bw) (corres...
Article
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Terbuthylazine belongs to the chloro-s-triazine group of herbicides and acts primarily as a photosynthesis inhibitor. The mechanisms of action related to its exposure, relevant both in animals and humans, are still insufficiently investigated. This comprehensive study focused on the outcomes of terbuthylazine exposure at cell level in vitro, and a...
Article
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Humans have used insecticides since ancient times. The spectrum and potency of available insecticidal substances has greatly expanded since the industrial revolution, resulting in widespread use and unforeseen levels of synthetic chemicals in the environment. Concerns about the toxic effects of these new chemicals on non-target species became publi...
Article
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Pesticides are a highly diverse group of compounds and the most important chemical stressors in the environment. Mechanisms that could explain pesticide toxicity are constantly being studied and their interactions at the cellular level are often observed in well-controlled in vitro studies. Several pesticide groups have been found to impair the red...
Poster
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Due to its high phenol content, strawberry tree honey possesses many beneficial health effects. The aim of this study was to evaluate potential DNA and cytoprotective effects of A. unedo honey after shortterm supplementation. Participants (six healthy men, aged 26-38) consumed 70 g of honey (from Pelješac peninsula, Croatia) dissolved in 200 mL of...
Article
In this 28 day-study, we evaluated the effects of the insecticide chlorpyrifos orally administered to Wistar rats at doses 0.160, 0.015, and 0.010 mg/kg b. w./day. Following treatment, total cholinesterase activity and activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were measured. Oxidative stress responses were evaluated...
Poster
Tembotrione is a selective, triketone herbicide used for post-emergence weed control in corn. Some pesticides may act as endocrine disrupting chemicals because of their strong potential to bind to oestrogen and androgen receptors and change sex hormone status in males and females. Especially vulnerable groups are foetuses, infants and children in p...
Article
Objective: This study aimed to evaluate possible DNA damages to oral epithelial cells exposed to whitening kinds of toothpaste considering the effect of conventional non-whitening toothpaste. Materials and methods: Sixty volunteers were assigned into three experimental groups, each of them using a different regular toothpaste for the initial 2 m...
Article
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We studied the toxic effects of glyphosate in vitro on HepG2 cells exposed for 4 and 24 h to low glyphosate concentrations likely to be encountered in occupational and residential exposures [the acceptable daily intake-ADI (0.5 μg/mL), residential exposure level-REL (2.91 µg/mL) and occupational exposure level-OEL (3.5 µg/mL)]. The assessments were...
Article
Background Diazepam, as one of the most frequent prescribed drug from 1,4-benzodiazepine group, has certain limitations in pharmaceutical technology due to its poor solubility in water. By forming inclusion complexes with 2-hydroxypropyl-β-cyclodextrin, diazepam's biopharmaceutical properties can be greatly improved. Aim Aim of this research was to...
Article
Terbuthylazine is a selective pre- and post-emergency chloro-triazine herbicide used for a broad spectrum of weed control. We evaluated the potential of low doses of terbuthylazine to induce oxidative stress and cytogenetic damage in peripheral blood samples of adult male Wistar rats. Following 28-day repeated oral exposure at 0.004 mg/kg b.w./day,...
Article
In parallel with the continuous use of conventional insecticides, introduction of more environmentally friendly substances continues to grow in modern agriculture. In the present study, we evaluated chlorpyrifos, and imidacloprid and α-cypermethrin as two representatives of green insecticides for their genotoxic activity. We conducted a 14-day trea...
Article
Objective: To investigate both genotoxicity and hardening of bulk-fill composite materials applied in 4-mm layer thickness and photo-activated for different exposure times. Methods: Three flowable bulk-fill materials and one conventional flowable composite were filled in molds (height: 4mm) and irradiated for 20 or 30s. The top (0mm) and bottom...
Article
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Nanoparticle use has increased radically raising concern about possible adverse effects in humans. Zinc oxide nanoparticles (ZnO NPs) are among the most common nanomaterials in consumer and medical products. Several studies indicate problems with their safe use. The aim of our study was to see at which levels ZnO NPs start to produce adverse cytoge...
Article
This study evaluated the cyto- and genotoxic effects of three pesticides: α-cypermethrin, chlorpyrifos and imidacloprid applied in vitro to human lymphocytes and HepG2 cells for exposure times of 4 and 24 h at concentrations corresponding to OEL, ADI and REL. Assessments were made using oxidative stress biomarkers and the alkaline comet, cytokinesi...
Poster
The huge expansion of genetically modified plants designed to tolerate high levels of glyphosate has made it the world’s most widely used herbicide. Nevertheless, there are controversies about its use; the WHO and IARC have been unable to reach an agreement with the EFSA regarding its further use and distribution ever since the former proclaimed gl...
Article
Tembotrione is a triketone herbicide, usually used for post-emergence weed control in corn. Currently, there is little or no published data on its genotoxicity to human cells either in vitro or in vivo. This study evaluated the impact of acute (4 and 24 h) exposure to low concentrations of tembotrione [corresponding to the acceptable daily intake (...
Article
In occupational exposures, populations are simultaneously exposed to a mixture of chemicals. We aimed to evaluate DNA damage due to possible carcinogen exposure (phenylhydrazine, ethylene oxide, dichloromethane, and 1,2-dichloroethane) in lymphocytes of pharmaceutical industry workers from the same production line. Population comprised 16 subjects...
Article
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Comet-FISH technique is an extension of commonly used comet assay. Its purpose is to determine whether primary DNA damage which comet assay detects occurred within a sequence of interest that is visualized by hybridization of fluorescent probe. Presence of the signal in comet tail indicates impaired structural integrity of sequence. Our modificatio...
Article
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Correction for ‘Bioactivity of Satureja montana L. honey extracts and their profile screening’ by Mladenka Malenica Staver et al., RSC Adv., 2014, 4, 47329–47340.
Article
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Exploitation of natural compounds, including those from the honeys, as therapeutic agents is still gaining importance, but problems may arise due to poor knowledge of molecular effects and correlation of these effects with doses. The aim of the study was to characterize the extracts from winter savory (Satureja montana L.) honey, evaluate their bio...
Conference Paper
Objective: Variety of resins has been introduced into dental practice for the manufacturing of dental prostheses and their efficacy has been based on physical, chemical and biological properties. Recently, 3D printing techniques have been introduced to manufacture new denture base materials. The aim of this study was to evaluate genotoxicity of n...
Conference Paper
Objective: The aim of the present study was to evaluate the genotoxicity of low-viscosity bulk-fill composite resins by measuring the level of DNA strand breaks and micronucleus frequency. Methods: Three flowable bulk-fill materials [x-tra base (XB), Surefil SDR flow (SDR), Venus Bulk Fill (VB)] and one conventional flowable control composite res...
Article
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Agricultural workers are often exposed to high levels of pesticides over prolonged periods of time. We attempted to determine whether exposure to multiple pesticides shortens relative telomere length (RTL) and causes nucleoplasmic bridge (NPB) formation via the mechanism of telomere-end fusion in the lymphocytes of agricultural workers. For measuri...
Article
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The purpose of this study was to evaluate the genotoxic potential of components leached from two conventional self-curing glass-ionomer cements (Fuji IX and Ketac Molar), and light-curing, resin modified glass-ionomer cements (Vitrebond, Fuji II LC). Evaluation was performed on human lymphocytes using alkaline and hOGG1 modified comet, and micronuc...
Article
K048 is a member of K-oximes, a new oxime class that has recently been confirmed effective against poisoning by the nerve agent tabun and several pesticides. The toxicity profile of the K048 oxime has not been fully characterized and its optimal therapeutic dose has not yet been established. Earlier studies report excellent results with K048 in rea...
Article
The aim of our study was to investigate how exposure to heavy metal-rich waters from gypsum mining affects the morphology and levels of primary DNA damage in Gammarus balcanicus. Chemical analysis revealed increased concentrations of metals in water and sediment collected at a site impacted by gypsum mine wastewaters. The specimens also showed elev...
Conference Paper
Objective: Adhesive resin cements have became popular due their dual-cured properties to meet specific needs of bonding of CAD/CAM restorations. The aim of this study was to compare cytotoxicity of a amine-free resin cement (RelyX Ultimate, 3M ESPE) polymerized under different curing times through ceramic-reinforced polymer (CRP) (LAVA Ultimate,...
Article
K048 is a member of K-oximes, a new oxime class that has recently been confirmed effective against poisoning by the nerve agent tabun and several pesticides. The toxicity profile of the K048 oxime has not been fully characterized and its optimal therapeutic dose has not yet been established. Earlier studies report excellent results with K048 in rea...
Conference Paper
Objective: The objective was to investigate the cytotoxicity and genotoxicity of four resin-based materials, monomers TEGDMA and Bis-GMA in vitro. Method: The tested materials were Tetric EvoCeram, Tetric EvoFlow, G-aenial and G-aenial Flo. First, they capability to induce cell death was tested. This in vitro investigation was performed on hu...
Article
Objective: The most important requirement for a material to be used in medical applications is its biocompatibility. Dental composite materials come into direct contact with oral tissues, especially gingival and pulpal cells. This study was performed to evaluate possible DNA damage in cells of human origin exposed to dental composites in vitro usi...
Article
This study investigates the effects of oxime K048 (730, 200, and 7.3nM) on the viability and chromosome stability of human peripheral blood lymphocytes (PBLs) after a 30min exposure in vitro. Cytotoxicity was tested by a viability assay with ethidium bromide and acridine orange. For the evaluation of the genotoxic potential, we used comet assays, c...
Article
Full-text available
Background/purposeThe use of dental adhesives in everyday dental practice has raised questions about their biologic safety. Their biocompatibility is a relevant aspect of the clinical success of these materials. The objective of this study was to evaluate the genotoxicity of dental adhesives ex vivo using a cytogenetic assay.Materials and methodsFo...