David Peyrade

David Peyrade
French National Centre for Scientific Research | CNRS · Institut des sciences de l'ingénierie et des systèmes (INSIS)

PhD

About

218
Publications
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2,673
Citations

Publications

Publications (218)
Article
Micrometric white light emitters were fabricated by applying two-photon polymerization (TPP) lithography to a chemically modified resist. Such a functional resist (FR) was doped with fluorescent molecules to turn it emitting into the whole visible region. The TPP has shown to be a versatile, low cost maskless technique capable to fabricate highly r...
Article
Because of antibiotics misuse, the dramatic growth of antibioresistance threatens public health. Tests are indeed culture‐based, and require therefore one to two days. This long time‐to‐result implies the use of large‐spectrum antibiotherapies as a first step, in absence of pathogen characterization. Here, a breakthrough approach for a culture‐less...
Article
Les oléosomes sont des gouttelettes lipidiques limitées par une monocouche de lipides associée à des protéines (oléosines), répandus dans les graines, notamment dans les oléagineux alimentaires comme l’arachide. Les oléosines (Ara h 10, Ara h 11, Ara h 14, Ara h 15) sont les protéines les plus abondantes des oléosomes. Elles sont constituées d’un c...
Article
Full-text available
Betacoronaviruses, responsible for the “Severe Acute Respiratory Syndrome” (SARS) and the “Middle East Respiratory Syndrome” (MERS), use the spikes protruding from the virion envelope to attach and subsequently infect the host cells. The coronavirus spike (S) proteins contain receptor binding domains (RBD), allowing the specific recognition of eith...
Conference Paper
Full-text available
In this paper, we present a platform composed of three transparent heater-sensor microsystems capable of maintaining a cell culture at a desired temperature. Accurate and stable temperature conditions are therefore accessible throughout the entire culture cycle.
Article
Allergic diseases have increased its incidence worldwide, increasing the significance of research in diagnostics to offer more precise immunotherapy options. Most current lines of work revolve around single-protein detection, which relies mostly on faint fluorescence signals and large expensive detectors. In this context, we propose a procedure bas...
Article
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Fast and label-free techniques to analyze viruses and bacteria are of crucial interest in biological and bio-medical applications. For this purpose, optofluidic systems based on the integration of photonic structures with microfluidic layers were shown to be promising tools for biological analysis, thanks to their small footprint and to their abili...
Conference Paper
We report on the optical trapping of seven types of living bacteria in 2D hollow photonic crystal cavities. The possibility of distinction of bacteria via the interaction effects with the trapping field will be discussed.
Conference Paper
We report on the optical trapping and Gram-type differentiation of seven types of living bacteria in 2D hollow photonic crystal cavities, thanks to the analysis of the membrane-dependent resonance frequency shift.
Conference Paper
We report on the on-chip real-time state monitoring of a single alive bacterium trapped with optical 1D microcavities. Through a two-laser setup, this label-free approach allows to monitor bacteria stressed by antibacterial agent (antibiotics, temperature).
Conference Paper
Nanoparticles, plates, fibers, can self-align using capillarity. This property has been demonstrated for nanoparticles, microelectronic chips and optical components of dimensions comprised between 20 nm, 500 μm and a few millimeters. Capillary self-assembly relies on the restoring forces and torques exerced by the surface tension of the free surfac...
Article
The assembly of semiconductor nanowires with nanoscale precision is crucial for their integration into functional systems. In this work, we propose a novel method to experimentally determine the real part of the Clausius-Mossotti factor (CMF) of silicon and silicon-germanium nanowires. The quantification of this CMF is measured with the nanowires v...
Article
Photonic crystals and microcavities act as on-chip nano-optical tweezers for identification and manipulation of biological objects. Until now, optical trapping of virus and bacteria has been achieved and their presence in the vicinity of the optical resonator is deduced by the shift in the resonant wavelength. Here, we show trapping and identificat...
Article
Dielectrophoresis is widely used for cell characterization, and the exerted force on cells depends on the difference of polarizability between the latter and the surrounding medium. This physical phenomenon is translated by the real part of the Clausius-Mossotti factor. It is mostly modeled from the imaginary part, measured by electrorotation. The...
Article
With the rise of microfluidics for the past decade, there has come an ever more pressing need for a low-cost and rapid prototyping technology, especially for research and education purposes. In this article, we report a rapid prototyping process of chromed masks for various microfluidic applications. The process takes place out of a clean room, use...
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Full-text available
By analyzing the thermal motion of fluorescent dielectric microbeads trapped in the near-field of a silicon nanocavity, we investigate the influence of the bead's size and the trapping laser power on the shape of the optical trap and the "effective" trap stiffness. We demonstrate that the trapping potential is proportional to the subwavelength patt...
Article
3D control of the motion of self-powered micromotors is demonstrated using AC electrokinetics by applying an AC electric field on indium tin oxide transparent electrodes. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Article
Dielectrophoresis is a force that has been exploited in microsystems for label-free characterization and separation of cells, when their electrical signature is known. However, the polarization effect of cells at the transmembrane protein level is not well established. In this work, we have use the self-rotation effect of cells in a non-rotating fi...
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Full-text available
We report here the optical trapping of a heterogeneous colloidal dimer above a photonic crystal nanocavity used as an on-chip optical tweezer. The trapped dimer consists of a cluster of two dielectric microbeads of different sizes linked by van der Waals forces. The smallest bead, 1 mu m in diameter, is observed to be preferentially trapped by the...
Article
Hematopoietic stem cells (HSCs) are the most commonly used cell type in cell-based therapy. However, the investigation of their behavior in vitro has been limited by the difficulty of monitoring these non-adherent cells under classical culture conditions. Indeed, fluid flow moves cells away from the video-recording position and prevents single cell...
Article
Full-text available
AC electrokinetics is a versatile tool for contact-less manipulation or characterization of cells and has been widely used for separation based on genotype translation to electrical phenotypes. Cells responses to an AC electric field result in a complex combination of electrokinetic phenomena, mainly dielectrophoresis and electrohydrodynamic forces...
Article
We used alternative electric fields inside an electro-microfluidic chip to handle and characterize human cells. We experimentally measured the factor related to the applied dielectrophoretic force (Clausius-Mossotti factor). We also observed and quantified a self-rotating effect on cells, that appeared around the crossover frequency. The forces at...
Article
When a large number of similar gold particles are organized into complex architectures, the dipolar plasmon spectrum of the individual plasmonic entities gives rise to a broader, red-shifted feature centered around 750 nm. In this work, we show that superstructures fabricated using the convective assisted capillary force assembly method (CA-CFA) an...
Article
Full-text available
We report on the fabrication and photovoltaic characterization of In0:12Ga0:88N/GaN multi-quantum-well (MQW) solar cells grown by metal– organic vapor phase epitaxy on (0001) sapphire substrates. Increasing the number of MQWs in the active region from 5 to 30 improves a factor of 10 the peak external quantum efficiency of the device at the price of...
Article
Full-text available
Particles manipulation with optical forces is known as optical tweezing. While tweezing in free space with laser beams was established in the 1980s, integrating the optical tweezers on a chip is a challenging task. Recent experiments with plasmonic nanoantennas, microring resonators, and photonic crystal nanocavities have demonstrated optical trapp...
Article
Full-text available
AC electrokinetics is becoming a strategic tool for lab-on-a-chip systems due to its versatility and its high level of integration. The ability to foreseen the behaviour of fluids and particles under non-uniform AC electric fields is important to allow new generations of devices. Though most of studies predicted motion of particles in co-planar ele...
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Full-text available
The design of a new hybrid magnetic MEMS for biology (Bio-Mag-MEMS) is presented. It combines magnetophoresis, dielectrophoresis and microfluidics. These phenomena are used to sort magnetically tagged and non-tagged objects. Magnetic objects are trapped by the use of high gradient magnetophoresis. Thanks to dielectrophoresis these objects, once tra...
Article
Full-text available
In this paper, we introduce a dielectrophoresis (DEP)-based handling method that allows fine 3D manipulation of beads in suspension using a lab on a chip device. The device consists of two layers of linear electrodes on the top and bottom of a microfluidic channel. Each electrode layer has a 53 × 53 donut trap matrix, with traps that are linearly c...
Article
Full-text available
A promising approach to improve the performance of microelectronic devices is to build three-dimensional (3D) chips made of stacked circuits. However, a major hurdle lies in the fabrication of dense arrays of electrical interconnections between these layers, where accessibility is limited. Here we show that the directed growth and self-organization...
Article
We investigate the influence of growth temperature, p -doping with bis-cyclopentadienyl magnesium (Cp2Mg) and number N of multi-quantum wells on the surface morphology, the electrical and optical properties of InGaN-based solar cells grown by metal-organic vapour phase epitaxy. Atomic force microscopy measurements show no influence of multiple-quan...
Article
Full-text available
This work determines the dielectrophoretic response of surface modified polystyrene and silica colloidal particles by experimentally measuring their Clausius-Mossotti factors. Commercial charged particles, fabricated ones coated with fibronectin, and Janus particles that have been grafted with fibronectin on one side only were investigated. We show...
Article
Full-text available
This work uses convective assisted capillary force assembly from a collection of 100 nm fluorescent polystyrene nanoparticles to fabricate 4 μm wide and 1 μm high localized light-emitting superstructures. Large scale superstructures of about 10 000 particles are transferred on free silicon surfaces by microcontact printing. Optical characterization...
Article
Full-text available
The design of a new hybrid magnetic MEMS for biology (Bio-Mag-MEMS) is presented. It combines magnetophoresis, dielectrophoresis and microfluidics. These phenomena are used to sort magnetically tagged and non-tagged objects. Magnetic objects are trapped by the use of high gradient magnetophoresis. Thanks to dielectrophoresis these objects, once tra...
Article
Full-text available
In this paper, we study the influence of fabrication uncertainties on silicon-on-insulator optical nanosystems performances. The considered structure consists in near-field coupled twin nanobeam cavities. We have performed experiments on a given population of samples, and noticeable changes in light propagation and localization were observed. Thank...
Article
The use of surface coating on biomaterials can render the original substratum with new functionalities that can improve the chemical, physical, and mechanical properties as well as enhance cellular cues such as attachment, proliferation, and differentiation. In this work, we combined biocompatible polydimethylsiloxane (PDMS) with a biomimetic polye...