David M. Charbonneau

David M. Charbonneau
Paraza Pharma · Department of Biology

Ph.D.

About

28
Publications
9,110
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876
Citations
Additional affiliations
September 2018 - present
Paraza Pharma
Position
  • Researcher
September 2013 - December 2016
Université de Montréal
Position
  • PostDoc Position
January 2012 - December 2018
Université du Québec à Trois-Rivières
Position
  • Lecturer
Description
  • 2018 Bioinformatics, Proteomics and Genomics (ABI1001) 2018 Systemic Biology of the Cell (BIO1004) 2012-2013 Experimental Biochemistry (BCM1004)
Education
January 2008 - December 2014
September 2005 - April 2008

Publications

Publications (28)
Article
Full-text available
We report that a shorter Debye length and, as a consequence, decreased colloidal stability are required for the molecular interaction of folic acid-modified Au nanoparticles (Au NPs) to occur on a surface-bound receptor, human dihydrofolate reductase (hDHFR). The interaction measured using surface plasmon resonance (SPR) sensing was optimal in a ph...
Article
Full-text available
Bacterial lipolytic enzymes were originally classified into eight different families defined by Arpigny and Jaeger (families I-VIII). Recently, the discovery of new lipolytic enzymes allowed for extending the original classification to fourteen families (I-XIV). We previously reported that G. thermodenitrificans EstGtA2 (access no. AEN92268) belong...
Article
Full-text available
Background Biodiesels are methyl esters of fatty acids that are usually produced by base catalyzed transesterification of triacylglyerol with methanol. Some lipase enzymes are effective catalysts for biodiesel synthesis and have many potential advantages over traditional base or acid catalyzed transesterification. Natural lipases are often rapidly...
Article
Full-text available
Recent investigations of Aneurinibacillus thermoaerophilus strains have allowed identification of a unique solvent tolerant lipase, distinct from known lipases. This work reports the expression and purification of this lipase (LipAT) and the first characterization of its structure and temperature and pH-dependent behaviour. LipAT has a secondary st...
Article
Full-text available
The clinical success of Escherichia coli l-asparaginase II (EcAII) as a front line chemotherapeutic agent for acute lymphoblastic leukemia (ALL) is often compromised because of its silent inactivation by neutralizing antibodies. Timely detection of silent immune response can rely on immobilizing EcAII, to capture and detect anti-EcAII antibodies. H...
Article
Background: Understanding the relationship between enzymatic stability and the amino acid sequence encoding carboxylesterases is of utmost importance. Objectives: Here we thoroughly characterized the behavior of the carboxylesterase EstGtA2 from Geobacillus thermodenitrificans during thermal denaturation at different pH with various salt concent...
Article
Monitoring the response of patients undergoing chemotherapeutic treatments is of great importance to predict remission success, avoid adverse effects and thus, maximise the patients’ quality of life. In the case of leukemia patients treated with E. coli L-asparaginase, monitoring the immune response by the detection of specific antibodies to L-aspa...
Article
Full-text available
Based on extensive phylogenetic analyses, and structural and functional criteria, it is more appropriate to subdivide the family XIII. Every instance of “family XV” in the paper should be replaced by “subfamily XIII.2” The correct title should be: Role of Key Salt Bridges in Thermostability of G. thermodenitrificans EstGtA2: Distinctive Patterns wi...
Article
Full-text available
Ten thermophilic bacterial strains were isolated from manure compost. Phylogenetic analysis based on 16S rRNA genes and biochemical characterization allowed identification of four different species belonging to four genera: , , and . PCR-RFLP profiles of the 16S-ITS-23S rRNA region allowed us to distinguish two subgroups among the . isolates. Isola...
Article
Full-text available
The novel thermostable carboxylesterase EstGtA2 from G. thermodenitrificans (accession no. AEN92268) was functionally expressed and purified using an N-terminal fusion tag peptide. We recently reported general properties of the recombinant enzyme. Here we report preliminary data on thermal stability of EstGtA2 and of its tagged form. Conformational...
Article
Full-text available
A novel gene encoding an esterase from Geobacillus thermodenitrificans strain CMB-A2 was cloned, sequenced and functionally expressed in Escherichia coli M15. Sequence analysis revealed an open reading frame of 747 bp corresponding to a polypeptide of 249 amino acid residues (named EstGtA2). After purification, a specific activity of 2.58 U mg−1 wa...
Article
There are several lipid binding sites on serum albumins. The aim of this study was to examine the binding of bovine serum albumin (BSA) to cholesterol (Chol), 1,2-dioleoyl-3-(trimethylammonium)propane (DOTAP), (dioctadecyldimethyl)ammonium bromide (DDAB), and dioleoylphosphatidylethanolamine (DOPE), at physiological conditions, using constant prote...
Article
Full-text available
Despite considerable interest and investigations on cationic lipid-DNA complexes, reports on lipid-RNA interaction are very limited. In contrast to lipid-DNA complexes where lipid binding induces partial B to A and B to C conformational changes, lipid-tRNA complexation preserves tRNA folded state. This study is the first attempt to investigate the...
Article
Human serum albumin (HSA) is a major transporter for delivering several endogenous compounds including fatty acids in vivo. Even though HSA is the primary target of fatty acid binding, the effects of cationic lipid on protein stability and conformation have not been investigated. The aim of this study was to examine the interaction of human serum a...
Article
Full-text available
Complexes of cationic liposomes with DNA are promising tools to deliver genetic information into cells for gene therapy and vaccines. Electrostatic interaction is thought to be the major force in lipid–DNA interaction, while lipid-base binding and the stability of cationic lipid–DNA complexes have been the subject of more debate in recent years. Th...

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