David Kalfert

David Kalfert
Charles University in Prague | CUNI · Department of Otorhinolaryngology, Head and Neck Surgery (1. LF)

MD., Ph.D.
Thyroid pathology (benign, cancer, miRNA etc.), head and neck cancer, salivary gland tumors, nanorobots etc.

About

105
Publications
56,635
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497
Citations
Introduction
Current research interest: head and neck cancer, thyroid neoplasm, salivary gland tumor, anosmia and ageusia in patients with Covid-19. My web projects: www.cich.info, www.slinnezlazy.cz, www.dotaznik-covid.cz
Additional affiliations
April 2016 - April 2020
Charles University in Prague
Position
  • Medical Doctor
October 2007 - December 2014
Fakultní nemocnice Hradec Králové
Position
  • Mediacl doctor - resident
Education
September 2001 - May 2007
Faculty of Medicine in Pilsen
Field of study
  • General Medicine

Publications

Publications (105)
Article
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Background Salivary gland carcinomas (SGC) are extremely rare malignancies with only limited treatment options for the metastatic phase of the disease. Treatment with anti-CD47 antibodies could represent a potent therapy for SGCs by promoting the phagocytic clearance of tumor cells through various mechanisms. However, the efficacy of anti-CD47 ther...
Conference Paper
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Cíl: Cílem pilotní studie bylo zjistit a následně analyzovat epidemiologická data o zhoubných nádorech příušní žlázy v ČR. Metodika a materiál: Do retrospektivní studie se zapojilo 20 pracovišť. Sběr dat probíhal od 1. 9. 2020 do 31.1.2021 formou vyplnění online formuláře na stránkách projektu www.slinnezlazy.cz. Do studie byli zařazeni pacienti pr...
Article
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Sinonasal teratocarcinosarcoma is a rare and locally aggressive malignant tumour of the nose and paranasal sinuses, which has histological features of teratoma and carcinosarcoma. It is more common in men without age predilection. It often relapses locally. Due to the rarity, the exact therapeutic procedure is not defined. In our case, we provide a...
Article
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Head and neck squamous cell carcinomas (HNSCC) belong among severe and highly complex malignant diseases showing a high level of heterogeneity and consequently also a variance in therapeutic response, regardless of clinical stage. Our study implies that the progression of HNSCC may be supported by cancer-associated fibroblasts (CAFs) in the tumour...
Article
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I read with great interest the article entitled “The Long-Term Effects of 12-Week Intranasal Steroid Therapy on Adenoid Size, Its Mucus Coverage and Otitis Media with Effusion: A Cohort Study in Preschool Children” by Zwierz et al. [...]
Article
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Purpose Warthin tumour (WT) management options comprise surgery or follow-up. The purpose of this study was to asses our experience with the follow-up strategy in selected patients with an ultrasound-guided fine-needle aspiration biopsy (FNAB) showing WT. Methods We performed a retrospective analysis of patients diagnosed with WT using FNAB betwee...
Article
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PD‐L1 and PD‐1 Expression in Thyroid Follicular Epithelial Dysplasia: Hashimoto Thyroiditis Related Atypia and Potential Papillary Carcinoma Precursor Programmed cell death ligand (PD‐L1)/PD‐1 expression has been studied in a variety of cancers and blockage of PD‐L1/PD‐1 pathway is a cornerstone of immunotherapy. We studied PD‐L1/PD‐1 immunohistoch...
Article
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Since Noninvasive Follicular Thyroid Neoplasm with Papillary-like Nuclear Features (NIFTP) was introduced as a new thyroid tumour entity, many studies, and meta-analyses on diagnosing NIFTP have been published. NIFTP-revised histopathological criteria emerged in 2018. NIFTP is defined as a histological entity and its diagnosis requires a careful hi...
Article
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Background: A low-risk thyroid tumour, non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) was introduced in 2016. NIFTP criteria require a thorough histological examination to rule out capsular and lymphovascular invasion, which denies the possibility of preoperative cytological diagnosis. Nevertheless, since the...
Article
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(1) Background: The Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) was introduced in 2018, bringing an organ-specific classification system for salivary gland cytopathology. The aim of present study is to evaluate the MSRSGC prospectively, based on a two-year experience in the tertiary care center pathology department, and evaluat...
Article
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Objective: Oropharyngeal/laryngeal carcinoma are common cancers of the upper aerodigestive system. Human papillomavirus (HPV) is described as the most frequent in the cancer of unknown primary. The presence of Helicobacter pylori (HP) in the oral cavity is discussed in some papers. The aim of study: To analyze the incidence of HPV and HP in orop...
Article
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Objective Oncocytic papillary cystadenomas (OPCs) of the larynx are rare benign cystic lesions that usually present as supraglottic masses arising from the laryngeal ventricles. OPCs are found in patients older than 60 years, with a female predominance. Symptoms vary from asymptomatic to hoarseness, dyspnea, and dysphagia; often, they mimic a laryn...
Article
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Follicular epithelial dysplasia (FED) is described as Hashimoto thyroiditis-related atypia and is thought to be a possible precancerous lesion. Dysplasia as an interface between normal state and carcinoma is described in a wide range of diseases and carcinogenesis chains. On the other hand, inflammation-related atypia and cancerogenesis is also wid...
Article
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Background: Acute acoustic trauma (AAT) ranks, among others, as one common cause of inner ear function impairment, especially in terms of military personnel, who are at an increased exposure to impulse noises from firearms. Aim of this study: 1. We wanted to demonstrate whether early treatment of AAT means a higher chance for the patient to impr...
Article
Introduction: Thyroid cytology is a widely accepted tool in the clinical triaging of nodular lesions. Cell blocks (CBs) can help in the diagnosis of atypical lesions, namely, thyroid Bethesda category of Atypia of undetermined significance/follicular lesion of undetermined significance (AUS/FLUS). Methods: In a series of 224 AUS/FLUS thyroid sam...
Article
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Tonsillectomy is a routine surgery in otorhinolaryngology and the occurrence of postoperative bleeding is not a rare complication. The aim of this retrospective, observational, analytic, cohort study is to compare the incidence of this complication for the most common indications. A group of patients indicated for tonsillectomies for peritonsillar...
Article
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Cancer-associated fibroblasts (CAFs) are one of the most abundant and critical components of the tumor stroma. CAFs can impact many important steps of cancerogenesis and may also influence treatment resistance. Some of these effects need the direct contact of CAFs and cancer cells, while some involve paracrine signals. In this study, we investigate...
Article
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Fabry disease (FD) is a lysosomal storage disorder caused by pathogenic mutations in the alpha-galactosidase A (AGALA) encoding gene region. This rare disease affects several organs including the cochlea-vestibular system. Tinnitus and sensorineural hearing loss (SNHL) are reported among otoneurological symptoms. Early and correct diagnosis of FD i...
Article
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MiR-34a belongs to the class of small non-coding regulatory RNAs and functions as a tumor suppressor. Under physiological conditions, miR-34a has an inhibitory effect on all processes related to cell proliferation by targeting many proto-oncogenes and silencing them on the post-transcriptional level. However, deregulation of miR-34a was shown to pl...
Article
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Background: Low-grade fibromyxoid sarcoma (LGFMS) is a rare malignant tumor. Moreover, only 3 cases of LGFMS originating from the nasal cavity and/or paranasal sinuses have been published so far. Methods: Two patients with histologically confirmed LGFMS of the maxillary sinus were primarily treated by open and endoscopic surgery. In one case adj...
Article
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PurposeThe treatment strategy of parotid gland tumours depends mainly on the histopathological type of the lesion. Fine-needle aspiration biopsy (FNAB) is recommended in preoperative diagnostics. The aim of the study was to evaluate the FNAB standing in the diagnostic algorithm of parotid gland lesions and to correlate FNAB results in relation to t...
Article
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IgG4‐positive plasma cells in Hashimoto thyroiditis: IgG4‐related disease or inflammation‐related IgG4‐positivity? Despite the interest of researchers in IgG4‐related disease (IgG4‐RD), many questions still remain unanswered regarding the thyroid gland. We aimed to clarify the relationship between IgG4‐positive plasma cells and the histopathologica...
Article
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Aims: Total laryngectomy still plays an essential part in the treatment of laryngeal cancer and loss of voice is the most feared consequence of the surgery. Commonly used rehabilitation methods include esophageal voice, electrolarynx, and implantation of voice prosthesis. In this paper we focus on a new perspective of vocal rehabilitation utilizin...
Article
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Nodular fasciitis is a relatively rare benign disease affecting connective tissue which, due to its rapid growth and similarities in the histological finding, can easily mimic malignant soft-tissue tumors. In the case report we present a case of nodular fasciitis in the facial region of the 64-year--old man. Clinical symptomatology, pathophysiology...
Article
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Introduction: Human papillomavirus (HPV) infection plays an important role in the etiopathogenesis of oropharyngeal squamous cell carcinomas. HPV detection in these tumours is a positive prognostic marker. The p16 protein expression, which is detected immunohistochemically, is an indirect marker of active HPV infection. Unlike in oropharyngeal car...
Article
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Primární vaskulitidy jsou vzácnou skupinou onemocnění s rozmanitými lokálními a systémovými projevy. Onemocnění může zasáhnout jakýkoli orgán, včetně oblastí spadajících do problematiky otorinolaryngologie. Projevy na hlavě a krku pak nejsou u řady patologických jednotek vzácnými a u některých vaskulitid jsou přímou součástí diagnostických kritérií...
Article
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The differential diagnosis of well-differentiated tumors of follicular cell origin remains the most problematic task in thyroid pathology. Specific morphologic criteria (capsular and/or vascular invasion, nuclear characteristics) are crucial in the diagnosis of these neoplasms. However, the assessment of malignant features is inconclusive in some c...
Article
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Purpose Warthin tumour (WT) is the second most common benign tumour of the parotid gland. The aim of this study was to assess the value of the FNAB in the diagnosis and treatment decision in patients with WT. Materials and methods We performed a retrospective study of patients treated for parotid gland mass between 2006 and 2016. Patients who unde...
Article
Background/Aim: The aim of this study was to analyze the pretreatment cytokeratin serum levels in head and neck squamous cell carcinoma (HNSCC) by three assays in relation to selected clinicopathological characteristics in an effort to find diagnostic/prognostic biomarkers for HNSCC and to determine the best assay. Patients and Methods: In total, 4...
Article
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Rhinocerebral or rhino-orbito-cerebral mucormycosis (also known as zygomycosis) is a rare invasive fungal infection. Characterised by acute onset, the condition is particularly prevalent in immunocompromised patients and can rapidly result in death. Diabetes mellitus, especially in its decompensated form, is the main predisposing factor. Primary cl...
Article
Background/aim: The aim of this study was to analyze the pretreatment cytokeratin serum levels in head and neck squamous cell carcinoma (HNSCC) by three assays in relation to selected clinicopathological characteristics in an effort to find diagnostic/prognostic biomarkers for HNSCC and determine the best assay. Patients and methods: In total, 4...
Article
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Cancer microenvironment plays an essential role in promoting the growth, proliferation and ability of cancer cells to establish metastatic deposits. This microenvi-ronment consists predominantly of cancer-associated fibroblasts and the extracellular matrix produced by them, immune cells (macrophages, granulocytes, mast cells and lymphocytes), blood...
Article
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Despite distinctive advances in the field of head and neck squamous cell cancer (HNSCC) biomarker discovery, the spectrum of clinically useful prognostic serum biomarkers is limited. As metabolic activities in highly proliferative transformed cells are fundamentally different from those in non-transformed cells, specific shifts in concentration of...
Article
Résumé Buts Cette étude compare l’efficacité de l’adénoïdectomie dans le traitement de l’otite séromuqueuse (OSM) chez les patients ayant différentes tailles de végétations et la relation entre la taille des végétations et les sécrétions de l’oreille moyenne. Matériels et méthodes Une adénoïdectomie et une paracentèse sans pose d’aérateur transty...
Article
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Silent sinus syndrome (SSS) is a rare disease of the maxillary sinus. It is characterized by idiopathic and spontaneous enophthalmos due to the collapse of the orbital floor and maxillary sinus atelectasis. In our case report we describe a 17 – teen years old women with and accidental finding of SSS. The absence of typical clinical symptoms, low pa...
Article
Aims: This study compares the efficacy of adenoidectomy on otitis media with effusion (OME) in patients with different size of adenoids and the connection between differently sized adenoids and middle ear effusion. Material and methods: Children with a history of at least 3 months' OME underwent adenoidectomy and myringotomy without the insertio...
Article
Aim In patients with primary ciliary dyskinesia (PCD), the release of nitric oxide (NO) is extremely low by epithelia of the nasopharynx and sinuses. Measurement of nasal NO (nNO) is recommended as a screening test for PCD. The study aimed to evaluate if adenoids affects nNO and may deteriorate the performance of the test. Methods In 48 nonallergi...
Article
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Adenoid cystic carcinoma accounts for approximately 25% of malignant gland tumors, while its primary occurrence in the skin is highly rare. About 100 cases of primary cutaneous adenoid cystic carcinoma have been described in the literature to date. Our case report describes a rare occurrence of adenoid cystic carcinoma of the left auricle in an 85-...
Article
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Background: Central to neoplastic transformation and tumor progression is alteration of the signaling pathways that control cell proliferation and apoptosis. The key mechanisms for this neoplastic process are genetic changes (mutations of cancer-related genes) and recently identified epigenetic changes that involve DNA methylation, chromatin remod...
Article
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Background/aim: Head and neck squamous cell carcinoma (HNSCC) includes tumors of various anatomical sites sharing multifactorial etiopathogenesis and generally dismal response to conventional treatment. The objective of this study was to determine the clinical significance of serum levels of insulin-like growth factor-1 (IGF-1) and insulin-like gr...
Article
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The goals of this retrospective cohort study were to compare the results of clinical and pathological TNM staging in patients with laryngeal squamous cell carcinoma and to determine the impact of the discordance on prognosis and treatment results. A total of 124 patients with laryngeal cancer, primarily indicated for surgical treatment, were enroll...
Article
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Canal wall down mastoidectomy is one of the most effective treatments for cholesteatoma. However, it results in anatomical changes in the external and middle ear with a negative impact on the patient´s quality of life. To provide complete closure of the mastoid cavity and normalize the anatomy of the middle and external ear we used human multipoten...
Poster
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Úvod. Hybridní zobrazení PET/MRI kombinuje zobrazení tkání lidského těla magnetickou rezonancí (MRI) se zobrazením látkové výměny pozitronovou emisní tomografií (PET). PET/MRI nachází uplatnění zejména u onkologický nemocných. Hlavní výhodou je snížení radiační zátěže u těchto pacientů, což je výhodou nejen při opakovaných vyšetřeních, ale zejména...
Poster
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Úvod. Cirkulující nádorové markery mohou být v klinické praxi významné v diagnostice a prognózování nádorů i v monitorování průběhu choroby. Jejich klinický přínos však není u některých nádorů jednoznačně stanovený. V naší práci jsme se zaměřili na markery ze skupiny solubilních cytokeratinových fragmentů (TPS, MonoTotal a Cyfra 21-1) a na posouzen...
Article
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Úvod: Cílem studie bylo posoudit expresi galektinu-3 ve vztahu ke klinicko-patologickým ukazatelům u glotického dlaždicobuněčného karcinomu hrtanu. Soubor a metodika: Retrospektivní studie. Do studie bylo zařazeno 58 pacientů, kteří byli primárně chirurgicky léčeni pro glotický dlaždicový karcinom hrtanu na Klinice otorinolaryngologie a chirurgie h...
Article
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Mezenchymální kmenové buňky (MSC) jsou využívány k regeneraci poškozené mezenchymální tkáně. Jsou schopny se diferencovat ve zralé buňky kostní, chrupavčité, nervové, svalové či vazivové. Jejich účinek spočíva jednak v náhradě poškozené tkáně, ale také v parakrinním působení bioaktivních molekul. Autoři prezentují literární přehled recentního výzku...
Article
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Background: The aim of this study was to determine the percentage of discordance between clinical (c) and pathological (p) TNM classifications in cases of oropharyngeal carcinoma and whether it influences recurrence rate and prognosis of primary disease. Materials and methods: Fifty-one patients with oropharyngeal carcinoma who underwent primary su...
Article
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Background: In the cholesteatoma surgery ossicles can be replaced to reconstruct middle ear function. It is important that these ossicles are free of squamous epithelium, to prevent residual disease. This study focuses on the histological findings of the malleus and incus harvested during cholesteatoma surgery. Materials and methods: Eighty midd...
Article
Multipotent mesenchymal stromal cells (MSC) are used for regeneration of injured mesenchymal tissue due to their self-renewal capacity and ability to differentiate into cells of mesenchymal tissue (bone, cartilage, muscle, fat, nerve or fibrous tissues). They regenerate various tissues through self-renewal, differentiation capacity, immune modulati...
Article
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Objective: MicroRNAs (miRs) are noncoding, single-stranded regulatory RNA molecules involved in the posttranscriptional regulation of gene expression. They control the development and maintenance of the diverse cellular processes including proliferation, differentiation, motility and apoptosis. Expression of miRs is tissue-specific and each alterat...
Thesis
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Prognostic and predictive markers of the head and neck tumors Introduction. Head and neck squamous cell carcinoma (HNSCC) is aggressive tumor with unfavorable prognosis despite the improved therapy in the last decades. HNSCC with its clinicopathological and biological heterogeneity remains difficult to be clinically managed. Therefore, a better und...
Article
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Background: The goal of this prospective study was to determine the frequency of micrometastases in patients with squamous cell carcinoma (SCC) of the oral cavity, pharynx and larynx in whom elective neck dissection was indicated (cN0). Patients and methods: A total of 12 patients (10 males and 2 females) were enrolled in the study. The age rang...
Poster
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In our comparative study, we present 7 cases of oncocytic papillary cystadenoma of the larynx and provide a review of the literature. The clinical findings, diagnostics and therapy of this rare disease are discussed.
Article
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Současným standardem chirurgické léčby papilárního karcinomu je totální tyroidektomie a terapeutická bloková disekce u pacientů s klinicky či dle zobrazovacích metod prokázanými uzlinovými metastázami. Existují však i názory doporučující elektivní centrální krční disekci. Argumenty pro jsou vysoký výskyt okultních uzlinových metastáz v centrálním k...
Article
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Background: The aim of this study was to review the bacteriology of deep neck infections (DNI) and identify the factors that influence the incidence of causative bacteria. Methods: A retrospective analysis of 634 patients with DNI was performed. Statistical analysis was used to compare the incidence of common pathogens in various conditions such as...
Article
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Conclusion: Patients with chronic obstructive pulmonary disease (COPD) more frequently suffer from nasal symptoms as well as upper respiratory tract obstruction compared with the healthy population. Objective: The relationship between chronic rhinosinusitis and bronchial asthma has been studied in detail in the past. In recent years, a limited n...
Article
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BACKGROUND/AIM: MicroRNAs (miRs) are non-coding RNA molecules regulating diverse cellular processes essential in carcinogenesis. Little is known regarding miRs in head and neck squamous cell cancer (HNSCC). The aim of the present study was to investigate miRs in relation to the clinico pathological features of site-specific HNSCC. MATERIALS AND MET...
Article
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Oxid dusnatý (NO) je produkován ve vysokých koncentracích v horních dýchacích cestách. Vysoké koncentrace NO mají toxický účinek na bakterie a viry, čímž napomáhají obraně sliznice respiračního traktu. V posledních letech roste ve světě zájem o měření nazálního oxidu dusnatého (nNO) u onemocnění horních dýchacích cest, např. u ciliární dyskineze, a...
Article
Nitric oxide (NO) is produced in high concentration in the upper respiratory tract. The high concentration of NO is toxic to bacteria and viruses and thus helps to defend the mucous membranes of upper respiratory tract. In recent years is an increasing worldwide interest in the measurement of nasal nitric oxide (nNO) in diseases of the upper respir...