David Orestes Guarin Bedoya

• Head of R&D at Vitala Technologies

The hyperpolarization of biological samples using dissolution-DNP has become an attractive method for the monitoring of fast chemical and enzymatic reactions using NMR by taking advantage of large signal increase. This approach is actively developing, but still needs key methodological breakthroughs to be used as an analytical method for the monito...

The hyperpolarization of biological samples using dissolution-DNP has become an attractive method for the monitoring of fast chemical and enzymatic reactions using NMR by taking advantage of large signal increase. This approach is actively developing, but still needs key methodological breakthroughs to be used as an analytical method for the monito...

Introduction: This study aimed to assess the potential efficacy of the SGLT2 inhibitor empagliflozin and oral ketone ester supplementation as treatments for heart failure with preserved ejection fraction (HFpEF), which is commonly associated with obesity and type 2 diabetes mellitus. Methods: Twenty-week-old obese ZSF-1 rats were initially fed a hi...

We report dissolution Dynamic Nuclear Polarization (d‐DNP) of [¹⁵N3]metronidazole ([¹⁵N3]MNZ) for the first time. Metronidazole is a clinically approved antibiotic, which can be potentially employed as a hypoxia‐sensing molecular probe using ¹⁵N hyperpolarized (HP) nucleus. The DNP process is very efficient for [¹⁵N3]MNZ with an exponential build‐u...

We report dissolution Dynamic Nuclear Polarization (d‐DNP) of [ ¹⁵ N 3 ]metronidazole ([ ¹⁵ N 3 ]MNZ) for the first time. Metronidazole is a clinically approved antibiotic, which can be potentially employed as a hypoxia‐sensing molecular probe using ¹⁵ N hyperpolarized (HP) nucleus. The DNP process is very efficient for [ ¹⁵ N 3 ]MNZ with an expone...

We report several inductively coupled RF coil designs that are very easy to construct, produce high signal-to-noise ratio (SNR) and high spatial resolution while accommodating life support, anesthesia and monitoring in small animals. Inductively coupled surface coils were designed for hyperpolarized ¹³ C MR spectroscopic imaging (MRSI) of mouse bra...

Hyperpolarization chemistry based on reversible exchange of parahydrogen, also known as Signal Amplification By Reversible Exchange (SABRE), is a particularly simple approach to attain high levels of nuclear spin hyperpolarization, which can enhance NMR and MRI signals by many orders of magnitude. SABRE has received significant attention in the sci...

Dissolution dynamic nuclear polarization (DDNP) is a versatile tool to boost signal amplitudes in solution-state nuclear magnetic resonance (NMR) spectroscopy. For DDNP, nuclei are spin-hyperpolarized "ex situ" in a dedicated DNP device and then transferred to an NMR spectrometer for detection. Dramatic signal enhancements can be achieved, enabling...

Dissolution dynamic nuclear polarization (D-DNP) experiments rely on the transfer of a sample between two high-field magnets. During this transfer, samples might experience passage through regions where the stray fields of the magnets are very weak, can approach zero, and even change their sign. This can lead to unexpected spectral features in spin...

Long-lived imbalances of spin state populations can circumvent fast quadrupolar relaxation by reducing effective longitudinal relaxation rates by about an order of magnitude. This opens new avenues for the study of dynamic processes in deuterated molecules. Here we present an analysis of the relaxation properties of deuterated methyl groups CD3. Th...

Dynamic nuclear polarization (DNP) embraces a family of methods to increase signal intensities in nuclear magnetic resonance (NMR) spectroscopy. Despite extensive theoretical work that allows one to distinguish at least five distinct mechanisms, it remains challenging to determine the relative weights of the processes that are responsible for DNP i...

Blazar emission models are usually divided into two big families, leptonic and hadronic, according to the particles which are responsible for the gamma-ray component of their spectral energy distributions. Even though leptonic models have been successful in explaining the gamma-ray emission from most blazars, hadronic models still present an intrig...

Blazar emission models are usually divided into two big families, leptonic and hadronic, according to the particles which are responsible for the gamma-ray component of their spectral energy distributions. Even though leptonic models have been successful in explaining the gamma-ray emission from most blazars, hadronic models still present an intrig...