David H. FresnoUniversity of Barcelona | UB · environ
David H. Fresno
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17
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Introduction
Publications
Publications (17)
Arbuscular mycorrhizal fungi may alleviate water stress in plants. Although several protection mechanisms have already been described, little information is available on how these fungi influence the hormonal response to water stress at an organ-specific level. In this study, we evaluated the physiological and hormonal responses to water stress in...
Sweet cherry is an important non-climacteric fruit with a high commercial interest, but exploitation of sweet cherry trees ( Prunus avium L.) in orchards is usually subject to important economic losses due to fruit decay by pathogenic fungi and other microorganisms. Sweet cherries development and ripening are characterized by profound physiological...
Fusarium oxysporum (Fo) is best known as a host‐specific vascular pathogen causing major crop losses. Most Fo strains, however, are root endophytes potentially conferring endophyte‐mediated resistance (EMR). EMR is a mechanistically poorly understood root‐specific induced resistance response induced by endophytic or nonhost pathogenic Fo strains. L...
A complex network of cellular receptors, RNA targeting pathways, and small-molecule signaling provides robust plant immunity and tolerance to viruses. To maximize their fitness, viruses must evolve control mechanisms to balance host immune evasion and plant-damaging effects. The genus Potyvirus comprises plant viruses characterized by RNA genomes t...
[This corrects the article DOI: 10.3389/fmicb.2018.02977.].
Resistance (R) genes and endophytic organisms can both protect plants against pathogens. Although the outcome of both processes is the same, little is known about the commonalities and differences between both immune responses. Here we set out to phenotypically characterize both responses in the tomato-Fusarium pathosystem, and to identify markers...
EMR and RMR reduce susceptibility to Fusarium wilt (Fol007). (A) Ten-days-old seedlings of Fol007-susceptible KG52201 and Fol007-resistant KG324 were root dip-inoculated with water (mock), Fol007 or a mixture of Fo47:Fol007 (25 replicates for Fo47:Fol007 co-inoculated KG52201 and Fol007-inoculated KG324 seedlings, 10 replicates for the control trea...
Fo47 inoculation affects accumulation of specific proteins. (A) Proteins that were detected in at least three out of four replicates where labeled as present. Treatments embraced mock-inoculated, Fo47-inoculated, Fo47:Fol007-coinoculated and Fol007-inoculated C32 susceptible tomato plants. The black circle marks proteins that were specifically pres...
List of PR-5 proteins found in xylem sap of both bioassays. Uniprot IDs, names of the proteins, average of LFQ intensities for every treatment of the two bioassays, predicted location, GO category, disulphide bonds and length of the proteins are depicted in the table. LFQ values are represented in Log10 scale.
EMR and RMR reduce susceptibility to Fusarium wilt (Fol007). (A) Ten-days-old seedlings of Fol007-susceptible KG52201 and Fol007-resistant KG324 were root dip-inoculated with water (mock), Fol007 or a mixture of Fo47:Fol007 (25 replicates for Fo47:Fol007 co-inoculated KG52201 and Fol007-inoculated KG324 seedlings, 10 replicates for the control trea...
List of xylem sap proteins whose relative abundance changes upon co-inoculation of tomato with Fo47:Fol007 comparing to Fo47-inoculated.
Overview of the peptides matching NP24 and PR-5x proteins.
Fo47 colonizes tomato stems (A) To monitor stem colonization by Fo47 3-weeks-post-inoculation, stem sections at the crown and cotyledon-level were placed on PDA plates (10 replicates). Plates were scanned after 4 days of incubation. (B) Fungal outgrowth of the stem sections plotted as a percentage of infected sections. The experiment was repeated t...
Fol007 proteins identified and label-free quantified in Bioassay 1. LFQ values are in Log10 scale and 3,9 represent the LFQ cutoff.
Tomato proteins identified and label-free quantified in Bioassay 2. Samples labeled as ‘Mock’ for the water control, and ‘Patho’ for Fol007-inoculated KG324 resistant tomato plants. DAPs are marked with +. LFQ values are represented in Log10 scale. Sequence coverage (%), molecular weight (KDa), GO description and SecretomeP and ApoplastP outputs ar...
Overview of the protein groups detected peptides overlapping with NP24 and PR-5x. Filled in green the LFQ intensities obtained by only using unique peptides for the protein quantification. LFQ intensities are represented in Log10 scale.
Tomato proteins identified and label-free quantified in Bioassay 1. Samples labeled as ‘Mock’ for the water control, and ‘Endo,’ ‘Coinoc’ and ‘Patho’ for Fo47-inoculated, Fo47:Fol007-coinoculated and Fol007-inoculated C32 susceptible tomato plants. DAPs are marked with +. LFQ values are represented in Log10 scale. Sequence coverage (%), molecular w...
Questions
Question (1)
I am planning an experiment in which I want to evaluate the effect of different treatments on arbuscular mycorrhizal activity. Traditional methods such as the line intercept method allow us to quantify mycorrhizal colonization, but it does not distinguish between metabolically active hyphae or dead/not active fungal material. Is there any feasible method to somehow quantify metabolic mycorrhizal actively? Thank you vrey much in advance.
