David Brown

David Brown
ImYoo

Doctor of Philosophy
CTO/Co-founder at ImYoo, building the tech infrastructure to make single-cell immune insights routine and accessible.

About

19
Publications
3,539
Reads
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235
Citations

Publications

Publications (19)
Article
Full-text available
Bone tissue harbors unique and essential physiological processes, such as hematopoiesis, bone growth, and bone remodeling. To enable visualization of these processes at the cellular level in an intact environment, we developed “Bone CLARITY,” a bone tissue clearing method. We used Bone CLARITY and a custom-built light-sheet fluorescence microscope...
Article
Full-text available
Sensory feedback is a critical aspect of motor control rehabilitation following paralysis or amputation. Current human studies have demonstrated the ability to deliver some of this sensory information via brain-machine interfaces, although further testing is needed to understand the stimulation parameters effect on sensation. Here, we report a syst...
Article
Solar system bodies such as comets and asteroids are known to eject material from their surface in the form of jets and plumes. Observations of these transient outbursts can offer insight into the inner workings and makeup of their originating body. However, the detection of and response to these events has thus far been manually controlled by grou...
Article
Recombinant adeno-associated viruses (rAAVs) are efficient gene delivery vectors via intravenous delivery; however, natural serotypes display a finite set of tropisms. To expand their utility, we evolved AAV capsids to efficiently transduce specific cell types in adult mouse brains. Building upon our Cre-recombination-based AAV targeted evolution (...
Conference Paper
Despite being one of the primary gene therapy delivery vehicles, adeno-associated viruses (AAVs) are limited in their specificity towards certain cell types implicated in disease. Recombinant AAVs (rAAVs) are addressing these limitations through both capsid engineering and gene regulatory approaches that alter viral tropism or viral expression patt...
Article
Full-text available
Engineered variants of recombinant adeno-associated viruses (rAAVs) are being developed rapidly to meet the need for gene-therapy delivery vehicles with particular cell-type and tissue tropisms. While high-throughput AAV engineering and selection methods have generated numerous variants, subsequent tropism and response characterization have remaine...
Preprint
Full-text available
Engineered variants of recombinant adeno-associated viruses (rAAVs) are being developed rapidly to meet the need for gene-therapy delivery vehicles with particular cell-type and tissue tropisms. While high-throughput AAV engineering and selection methods have generated numerous variants, subsequent tropism and response characterization have remaine...
Article
Full-text available
An individual's immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enab...
Preprint
Full-text available
An individual’s immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enab...
Conference Paper
Adeno-associated viruses (AAVs) are widely used gene delivery vectors due to their ability to transduce dividing and non-dividing cells, their long-term persistence, and low immunogenicity. However, natural AAV serotypes have a limited set of tropisms. Directed evolution has been used to engineer recombinant AAVs to target specific cell types and t...
Conference Paper
Adeno-associated viruses (AAVs) are popular gene delivery vehicles and there is a continuing high demand for AAV variants with improved transduction efficiency and specificity. Directed evolution and/or rational design have been used extensively to engineer the capsid of naturally occurring AAVs in order to better customize their properties for res...
Conference Paper
Recombinant adeno-associated viral (rAAV) capsids are increasingly used as gene delivery vectors in science and in therapeutics. However, there is room for further improvement on the efficiency and specificity to transduce the central nervous system (CNS) via non-invasive systemic delivery. We have shown in the past that marked improvement is possi...
Conference Paper
Recombinant adeno-associated viral (rAAV) capsids are widely accepted as safe gene delivery vehicles in research laboratories and in gene therapy clinical trials and there is potential to further improve their usage by evolving the surface of the capsids to enhance their affinity to specific cell-types or tissues after intravenous (IV) delivery. To...
Conference Paper
Recombinant adeno-associated viruses (rAAVs) are commonly used as gene delivery vehicles in biomedical research and have shown great potential for gene therapy. Systemic administration of AAVs can be used to achieve broad vector distribution. However, key challenges remain with systemic administration of AAVs as their lack of organ and/or cell-type...
Article
Objective: Typically, clinical measures of cognition require motor or speech responses. Thus, a significant percentage of people with disabilities are not able to complete standardized assessments. This situation could be resolved by employing a more accessible test administration method, such as a brain-computer interface (BCI). A BCI can circumv...
Article
A non-invasive brain-computer interface (BCI)-adapted version of the Peabody Picture Vocabulary Test - 4th Edition (PPVT-IV) was created with the overall goal of enabling test participation by people unable to make the speech or movement responses required for the unmodified test. The BCI-adapted PPVT-IV used custom algorithms to wait for the parti...

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Projects

Projects (3)
Project
We want high-resolution, temporal sampling of the human immune system to become a routine preventative and diagnostic health procedure, done from home without the need for a clinic or phlebotomist. By allowing people to self-collect blood, we can capture the state of the immune systems at times missed by traditional clinical studies, and empower people to be active participants in the scientific discovery process.
Project
To build an architecture for managing hundreds to thousands of single-cell sequencing datasets in an interactive way.