David Bravo

David Bravo
Nutreco

PhD

About

112
Publications
129,815
Reads
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3,612
Citations
Additional affiliations
January 2015 - present
Pancosma
Position
  • Chief Innovation Officer
September 2006 - December 2014
Pancosma
Position
  • R&D Director
September 1997 - September 2006
Inzo
Position
  • R&D Manager
Education
September 1998 - September 2001
AgroParisTech
Field of study
  • Nutrition
September 1997 - September 1998
Aix-Marseille Université
Field of study
  • Molecular and cellular nutrition
September 1994 - September 1997
Institut National Agronomique, Paris-Grignon
Field of study
  • Nutrition

Publications

Publications (112)
Article
Substantial economic losses in animal agriculture result from animals experiencing heat stress (HS). Pigs are especially susceptible to HS, resulting in reductions in growth, altered body composition, and compromised substrate metabolism. In this study, an artificial high-intensity sweetener and capsaicin (CAPS-SUC; Pancosma, Switzerland) were supp...
Article
Full-text available
Background: There is a great demand for antibiotic alternatives to maintain animal health and productivity. The objective of this experiment was to determine the efficacy of dietary supplementation of a blood group A6 type 1 antigen oligosaccharides-based polymer (Coligo) on growth performance, diarrhea severity, intestinal health, and systemic im...
Article
The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on blood profiles of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatm...
Article
The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on growth performance, diarrhea, and fecal β-hemolytic coliforms of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment ro...
Article
Full-text available
Dietary organic selenium (Se) is commonly utilized to increase formation of selenoproteins, including the major antioxidant protein, glutathione peroxidase (GPx). Inorganic Se salts, such as sodium selenite, are also incorporated into selenoproteins, and there is evidence that nanoelemental Se added to the diet may also be effective. We conducted t...
Article
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Background: Our previous study showed that 3 plant extracts enhanced the immune responses and growth efficiency of weaned pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV), which is one of the most economically important disease in swine industry. However, each plant extract differently effected on growth efficiency an...
Article
Selenium nanoparticles (SeNPs) have been suggested for various applications such as food, animal feed and biomedicine. However, thus far, wet chemical synthesis cannot produce sufficient quantities of SeNPs at the required quality. In this study, reactive aerosol processing is employed to produce SeNPs as powder concentrates of 2.2 to 4.0% (dry wei...
Article
Full-text available
Pigs exposed to elevated ambient temperatures exhibit reduced daily gain, alterations in muscle and fat deposition, and decreased health. Negative aspects of gastrointestinal (GI) function, integrity and permeability also occur. High intensity sweeteners can ameliorate the negative effects of heat stress (HS) by increasing GI glucagon-like peptide...
Article
Full-text available
The study investigated antioxidant effects of selenium (Se) on resilience to diquat-induced oxidative stress in nursery pigs. Thirty-five weaned pigs were individually housed and randomly assigned to one of five treatments. Pigs were 1) fed a basal diet and intraperitoneally injected with sterile saline (negative control, NC), 2) fed the basal diet...
Article
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1. This study was conducted to determine the effect of different sources of selenium (Se) on breast and liver tissue deposition, apparent metabolisable energy (AME), growth performance and antioxidant status of broilers, measured as Se content in liver and breast tissues and glutathione peroxidase (GSH-Px) in blood, when used in 0–35 d broiler chic...
Article
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Absorption of glucose, via intestinal Na⁺/glucose cotransporter 1 (SGLT1), activates salt and water absorption and is an effective route for treating Escherichia coli (E. coli)-induced diarrhea. Activity and expression of SGLT1 is regulated by sensing of sugars and artificial/natural sweeteners by the intestinal sweet receptor, T1R2-T1R3 expressed...
Article
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Subtherapeutic levels of dietary antibiotics increase growth performance in domestic animals, but the mechanisms are poorly understood. Here, 1-week-old broiler chickens were challenged with LPS (experiment 1), or co-infected with Eimeria maxima and Clostridium perfringens as an experimental model of necrotic enteritis (experiment 2), and fed a sta...
Article
Full-text available
Fish production is increasingly important to global food security. A major factor in maintaining health, productivity and welfare of farmed fish is the establishment and promotion of a stable and beneficial intestinal microbiota. Understanding the effects of factors such as host and environment on gut microbial community structure is essential for...
Chapter
Full-text available
This chapter presents information obtained from experiments involving male Ross 308 broiler chickens on the effects of a standardised combination of plant extracts (PE) including carvacrol, cinnamaldehyde and capsicum oleoresin, on bird performance, hepatic antioxidant concentration and immunomodulation. Birds were reared under industry recognised...
Article
Full-text available
The Na+/glucose cotransporter 1, SGLT1 is the major route for transport of dietary glucose from the lumen of the intestine into absorptive enterocytes. Sensing of dietary sugars and artificial sweeteners by the sweet taste receptor, T1R2-T1R3, expressed in the enteroendocrine L-cell regulates SGLT1 expression in neighboring absorptive enterocytes....
Article
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This study aimed to assess the effect of a commercial blend of phytogenic feed additives (PA), comprising 5% carvacrol, 3% cinnamaldehyde, and 2% capsicum oleoresin on the modulation of immune biomarkers of broiler chickens, their growth performance, dietary energy, and nutrient retention. Four-hundred day-old birds were assigned to one of four die...
Article
Full-text available
The aim of this study was to test the hypothesis of an improved growth, dietary nutrient availability and overall health of broiler chickens reared on recycled litter when fed a standardised combination of essential oils (EO; carvacrol, cinnamaldehyde and capsicum oleoresin). To assess the effect of dietary treatments, feed intake, weight gain, fee...
Article
Several endogenous antioxidant enzymes are selenoproteins. Supplementing appropriate amount of selenium (Se) in feed may contribute to neutralize cellular reactive oxygen species, therefore improving animal health and performance. We aimed to evaluate different sources of Se in mitigating diquat-induced oxidative stress in weaned pigs. Thirty-five...
Article
Supplementing selenium (Se) could boost immune responses against certain pathogens. The objective of this experiment was to evaluate different sources of Se supplements on growth performance and immunity of weaned pigs in response to diquat injection. Thirty-five crossbred pigs (9.72 ± 1.39 kg) were individually housed and randomly assigned to one...
Article
Rumen microbes have been characterized as two distinct groups; feed-associated and luminal microbes and epithelial rumen wall microbes. The rumen wall environment has been relatively understudied regarding microbial taxonomy and function. Rumen microbial fermentations provide key metabolic products for the host such as short chain fatty acids, whic...
Article
Full-text available
Background Magnolia tree bark has been widely used in traditional Asian medicine. However, to our knowledge, no studies have been reported investigating the effects of dietary supplementation with magnolia bark extract in chickens. Objective We tested the hypothesis that dietary supplementation of chickens with a Magnolia officinalis bark extract...
Article
Pigs exposed to elevated ambient temperatures exhibit reduced average daily gain, alterations in muscle and fat deposition during growth phases, and decreased overall health. Negative aspects of gastrointestinal (GI) function, integrity and permeability also result from hyperthermia. Artificial sweeteners have the potential to ameliorate the negati...
Article
Full-text available
Recent studies reveal complex patterns of hormone co-expression within enteroendocrine cells (EEC), contrary to the traditional view that gut hormones are expressed individually in EEC. Moreover, different hormones have been found in separate subcellular vesicles. However, detailed analysis of relative expression of multiple hormones has not been m...
Article
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Plants produce an extensive array of organic compounds derived from secondary metabolism that may be useful in animal nutrition because of their chemical makeup. These plant-derived bioactive compounds, also referred to as phytonutrients (PN) or phytobiotics, have been shown to express antimicrobial activities against a wide range of bacteria, yeas...
Article
Glucagon-like peptide 2 (GLP-2) therapy was shown previously to reduce inflammation-related gut damage from coccidiosis in dairy calves, and feeding of artificial sweetener stimulates GLP-2 secretion from intestinal L cells. The purpose of this study was to determine whether GLP-2 treatment or artificial sweetener feeding beginning 1 wk before an e...
Article
Full-text available
The objective of this experiment was to investigate the effects of rumen-protected Capsicum oleoresin (RPC) on productivity and immune responses including feed intake, milk yield and composition, white and red blood cells, lipid peroxidation, and blood concentration of cortisol, haptoglobin, glucose, and insulin in lactating dairy cows experimental...
Article
Full-text available
The objective of this experiment was to investigate the effects of rumen-protected Capsicum oleoresin (RPC) supplementation on feed intake, milk yield and composition, nutrient utilization, fecal microbial ecology, and responses to a glucose tolerance test in lactating dairy cows. Nine multiparous Holstein cows were used in a replicated 3 × 3 Latin...
Article
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Mucosa-associated microbial populations of the gastrointestinal tract are in intimate contact with the outer mucus layer. This proximity offers these populations a higher potential, than lumenal microbiota, in exerting effects on the host. Functional characteristics of the microbiota and influences of host- physiology shape the composition and acti...
Article
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TRPA1 is a ligand-activated cation channel found in the intestine and other tissues. Components of food that stimulate TRPA1 receptors (phytonutrients) include allyl isothiocyanate, cinnamaldehyde and linalool, but these may also act at other receptors. Cells lining the intestinal mucosa are immunoreactive for TRPA1 and Trpa1 mRNA occurs in mucosal...
Article
Full-text available
Numerous endocrine cell subtypes exist within the intestinal mucosa and produce peptides contributing to the regulation of critical physiological processes including appetite, energy metabolism, gut function, and gut health. The mechanisms of action and the extent of the physiological effects of these enteric peptides are only beginning to be uncov...
Article
Full-text available
The majority of 5-HT (serotonin) in the body is contained in enteroendocrine cells of the gastrointestinal mucosa. From the time of their discovery over 80 years ago, the 5-HT-containing cells have been regarded as a class of cell that is distinct from enteroendocrine cells that contain peptide hormones. However, recent studies have cast doubt on t...
Article
Heat stress compromises intestinal epithelial barrier integrity of mammals through mechanisms that may include oxidative stress. Our objective was to test whether dietary supplementation with antioxidants, selenium (Se) and Vitamin E (VE), protects intestinal epithelial barrier integrity in heat stressed pigs. Female growing pigs (n = 48) were rand...
Article
Full-text available
Three commercial broiler breeds were fed from hatch with a diet supplemented with Capsicum and Curcuma longa oleoresins, and co-infected with Eimeria maxima and Clostridium perfringens to induce necrotic enteritis (NE). Pyrotag deep sequencing of bacterial 16S rRNA showed that gut microbiota compositions were quite distinct depending on the broiler...
Article
Full-text available
This study investigated the effect of Capsicum oleoresin in granular form (CAP) on nutrient digestibility, immune responses, oxidative stress markers, blood chemistry, rumen fermentation, rumen bacterial populations, and productivity of lactating dairy cows. Eight multiparous Holstein cows, including 3 ruminally cannulated, were used in a replicate...
Article
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We discuss the relations of processed foods, especially cooked foods, in the human diet to digestive tract form and function. The modern consumption of over 70 % of foods and beverages in highly refined form favours the diet-related classification of humans as cucinivores, rather than omnivores. Archaeological evidence indicates that humans have co...
Article
Disruption in stable establishment of commensal gut microbiota by early weaning is an important factor in susceptibility of young animals to enteric disorders. The artificial sweetener SUCRAM [consisting of neohesperidin dihydrochalcone (NHDC) and saccharin] included in piglets' feed reduces incidence of enteric disease. Pyrosequencing of pig caeca...
Article
Full-text available
The ban on the use of antibiotics as growth promoters in animal feeds in the European Union has stimulated research on potential alternatives. Recently, propyl-propane thiosulfonate (PTSO), a stable organosulfurate compound of garlic, was purified. The objectives of the current study were to investigate the potential effects of PTSO on rumen microb...
Article
Full-text available
The aim of the study was to investigate the effects of a standardized mixture of a commercial blend of phytogenic feed additives containing 5% carvacrol, 3% cinnamaldehyde, and 2% capsicum on utilization of dietary energy and performance in broiler chickens. Four experimental diets were offered to the birds from 7 to 21 d of age. These included 2 b...
Article
Full-text available
Plant extracts, or phytonutrients, are used in traditional medicine practices as supplements to enhance the immune system and gain resistance to various infectious diseases and are used in animal production as health promoting feed additives. To date, there are no studies that have assessed their mechanism of action and ability to alter mucosal imm...
Article
Full-text available
An experiment was conducted to compare the effect of a supplementary mixture of essential oils, with and without exogenous xylanase, on performance, carcass composition, dietary nitrogen (N)-corrected apparent metabolizable energy (AMEn), dry matter retention (DMR), N retention (NR), fat digestibility (FD) coefficients, and endogenous mucin losses...
Article
Full-text available
The digestive systems of all species have been shaped by environmental pressures over long evolutionary time spans. Nevertheless, all digestive systems must achieve the same end points, the ingestion of biological material and its conversion to molecules that serve as energy substrates and structural components of tissues. A range of strategies to...
Article
Full-text available
The digestive systems of all species have been shaped by environmental pressures over long evolutionary time spans. Nevertheless, all digestive systems must achieve the same end points, the ingestion of biological material and its conversion to molecules that serve as energy substrates and structural components of tissues. A range of strategies to...
Article
Full-text available
The effects of dietary supplementation of young broiler chickens with an organic selenium (Se) formulation, B-Traxim Se, on experimental necrotic enteritis (NE) were studied. Chickens treated with three Se doses (0.25, 0.50, 1.00 mg/kg) from hatch were orally challenged with Eimeria maxima at 14 days of age followed by Clostridium perfringens to in...
Article
Full-text available
Abstract 1. This study was to evaluate the effects of supplementary dietary selenium (Se) given as sodium selenite on host immune response against necrotic enteritis (NE) in commercial broiler chickens. 2. Chicks were fed from hatching on a non-supplemented diet or diets supplemented with different levels of Se (0.25, 0.50 and 1.00 Se mg/kg). To in...
Article
Full-text available
This study has investigated the patterns of colocalisation of the conventional K cell marker, glucagon-like insulinotropic peptide (GIP), and the L cell markers, glucagon like peptide-1 (GLP-1) and peptide YY (PYY), in enteroendocrine cells (EEC) of the small intestine and colon of mouse and pig. All combinations of the hormones, 3 in a cell, 2 in...
Article
Full-text available
Plant extracts (PE) are naturally occurring chemicals in plants, and many of these molecules have been reported to influence production efficiency of dairy and beef animals. Two experiments were conducted to determine the effect of a PE additive (CE; an encapsulated blend of cinnamaldehyde and eugenol) on the milk production performance of lactatin...
Conference Paper
Full-text available
Abstract Text: The immune system of pigs is vital as its proper functioning protects the pig from disease and health. It also causes inflammation, which contributes to the animal’s ability to fight off infection but also inhibits growth performance by reducing feed intake and diverting amino acids and nutrients away from growth to the immune respon...
Conference Paper
Abstract Text: Intestinal L cells have key roles in the detection of the chemical environment in the gut lumen, to which they react by the release of hormones that influence appetite, proximal gut motility, insulin secretion and mucosal function (Furness et al, Nature Gastroenterology, 10, 729–740, 2013). Important amongst L cell hormones are glu...
Article
Full-text available
This study was conducted to characterize the effects of feeding 3 plant extracts on gene expression in ileal mucosa of weaned pigs. Weaned pigs (n = 32, 6.3 ± 0.2 kg BW, and 21-d-old) were housed in individual pens for 9 d and fed 4 different diets: a nursery basal diet as control diet, basal diet supplemented with 10 ppm of capsicum oleoresin, gar...