Dashnor Nebija

Dashnor Nebija
University of Prishtina · Department of Pharmacy

PhD

About

48
Publications
23,954
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253
Citations
Additional affiliations
January 1997 - present
University of Prishtina
Position
  • As. Professor

Publications

Publications (48)
Article
Microbiota is known to play a pivotal role in generating bioavailable and bioactive low-molecular-weight metabolites from dietary polyphenols. 5-O-caffeoylquinic acid (5-CQA), one of the main polyphenols found in human diet, was submitted to a resting cell biotransformation study using three gut bacteria species Lactobacillus reuteri, Bacteroides f...
Article
Full-text available
The study evaluates the chemical composition of the volatile constituents of ten plant species traditionally used as herbal tea in the Sharri Mountain regions (Kosovo and North Macedonia). Volatile constituents responsible for the flavour and fragrance of selected species (Crataegus monogyna, Cydonia oblonga, Malus sylvestris, Matricaria chamomilla...
Article
Full-text available
Six insulin analogues with high degree of chemical similarity were separated using Capillary Electrophoresis (CE). In order to improve the method performance, optimization and development the Design of Experiment (DoE) approach has been used and this strategy provided related statistical models. This methodology delivered the information regarding...
Article
Full-text available
The principal aim of this study was to determine the chemical composition of the essential oils obtained from needles and twigs of silver fir (Abies alba Mill.) and Bulgarian fir (Abies borisii-regis Mattf.) growing in Sharr Mountain, Kosovo. GC-MS and GC-FID were used for qualitative and quantitative analysis of volatile secondary metabolites in t...
Technical Report
Full-text available
Two capillary electrophoresis (CE) methods have been developed for the separation of charge and mass variants of human insulin and its recombinant analogue lispro. Since the capillary zone (CZE) and Capillary gel electrophoresis (CGE) are based on different principles of separation, they can be used to detect different impurities of insulin and its...
Article
Full-text available
The aim of this study was to assess variation of the chemical composition of essential oils obtained from inflorescences and leaves of Tilia platyphyllos Scop. collected from five different localities in Kosovo. GC-MS/GC-FID analysis revealed the presence of 96 compounds in essential oils obtained from inflorescences of large-leaved lime with the m...
Article
Full-text available
In the present study, eight plant species belonging to Lamiaceae family were identified as ingredients for herbal teas in the region of Sharri Mountains: Thymus serpyllum , Rosmarinus officinalis , Melissa officinalis, Origanum vulgare , Mentha longifolia , Ocimum basilicum , Teucrium chamaedrys , and Sideritis scardica, respectively. Chemical comp...
Article
Objective: Formulations containing probiotics are promoted due to health benefits. During lyophilization and subsequent storage in the gastrointestinal tract, bacteria are exposed to stress conditions that can lead to impairment and loss of viability. Methods: The suitability of various excipients for enhancing the stability and functionality of Bi...
Article
Full-text available
Aspergillus flavus is one of the most important mycotoxigenic species from the genus Aspergillus, due to its ability to synthesize the potent hepatocarcinogen, aflatoxin B1. Moreover, this fungus is capable of producing several other toxic metabolites from the class of indole-tetramates, non-ribosomal peptides, and indole-diterpenoids. Populations...
Article
Glycogen synthase kinase-3 (GSK-3) is a highly evolutionarily conserved and ubiquitously expressed serine/threonine kinase, an enzyme protein profoundly specific for glycogen synthase (GS). GSK-3 is involved in various cellular functions and physiological processes, including cell proliferation, differentiation, motility, and survival as well as gl...
Conference Paper
The genus Pinus (Pinaceae) in Kosovo is represented by 5 pine species (Pinus heldreichii, P. mugo, P. peuce, P. sylvestris dhe P. nigra), of which P. heldreichii and P. peuce are Balkans endemics. Pinus species contain different secondary metabolites, whose essential oils are the most important. With the aim to analyze the variability of the chemic...
Conference Paper
Phytoestrogens are a class of xenoestrogens which demonstrate a significant affinity to estrogen receptors. The principal classes of phytoestrogens, such as isoflavones, lignans, and coumestans are compounds present in many medicinal plants, dietary supplements and foods. These compounds are not only selective oestrogen receptor modulators (SERM),...
Article
Full-text available
The principal aim of this study was to investigate the chemical composition of volatile and phenolic compounds obtained from ripe fruits of European wild apple (Malus sylvestris (L.) Mill.) originating from Kosovo, as well as their antioxidant activity. Gas chromatograph equipped with Flame Ionisation Detector (GC/FID) and Gas chromatograph coupled...
Article
Full-text available
Atorvastatin, as 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, is a widely prescribed medication for the treatment of dyslipidemia. However, despite its clinical efficacy in reducing major cardiovascular events, a wide inter-individual variability in its response exists. Several studies in this area point to the effect of pol...
Article
Full-text available
Cyclopiazonic acid (α-cyclopiazonic acid, α-CPA) is an indole-hydrindane-tetramic acid neurotoxin produced by various fungal species, including the notorious food and feed contaminant Aspergillus flavus. Despite its discovery in A. flavus cultures approximately 40 years ago, its contribution to the A. flavus mycotoxin burden is consistently minimiz...
Article
Full-text available
The principal aim of this study was to analyze the chemical composition and qualitative and quantitative variability of essential oils obtained from seven naturally grown populations of the Pinus peuce Grisebach, Pinaceae in Kosovo. Plant materials were collected from three populations in the Sharri National Park and from four other populations in...
Conference Paper
Full-text available
The fluoroquinolones tend to be more soluble in water at acidic and/or basic pH (Andriole, 2000; Riley et al. 1989), which significantly affects their permeability. It has been well explained that solubility, dissolution and gastrointestinal permeability are fundamental parameters that control rate and extent of drug absorption and its bioavailabil...
Conference Paper
Full-text available
N-Mannich bases are of particular interest in drug chemistry because of their potential to be used as prodrugs of NH-acidic drugs containing amide, imide, ureide and amine moieties (Bala et al., 2014; Stella et al., 2007). With respect to the amines, N-Mannich bases are useful when an increase in their lipophilicity is required. Lipophilicity is on...
Conference Paper
Full-text available
Introduction Atorvastatin is a potent inhibitor of the 3-hydroxy- 3-methylglutaryl-coenzyme A reductase and it is wide¬ly used in the treatment of hypercholesterolemia and hy¬pertriglyceridemia (Fu et al., 2013). However, despite its clinical efficacy, great variability in clinical response ex¬ists. Much attention is paid to the genetic factors af...
Article
Ripe cones of Juniperus communis L. (Cupressaceae) were collected from five wild populations in Kosovo, with the aim of investigating the chemical composition and natural variation of essential oils between and within wild populations. Ripe cones were collected, air dried, crushed, and the essential oils obtained by hydrodistillation. The essential...
Article
Medicinal products obtained by recombinant DNA technology are complex molecules and demonstrate a high degree of molecular heterogeneity. Charge heterogeneity and isoform pattern of this class of medicines, are parameters important for their quality, safety, and efficacy. In this study we report the application of two-dimensional gel electrophoresi...
Article
Full-text available
OATP1B1 is an influx transporter known to mediate the uptake of various endogenous compounds and xenobiotics. Several sequence variations have been discovered in the SLCO1B1 gene encoding OATP1B1. The aim of this study was to investigate the effects of SLCO1B1 polymorphisms on the pharmacokinetics of atorvastatin in healthy volunteers of Macedonian...
Conference Paper
Biosimilar is a biological medicine that contains a version of the active substance of an already authorized, original biological medicinal product (reference medicinal product). A biosimilar demonstrates similarity to the reference medicinal product in terms of quality, biological activity, safety and efficacy. In the last decade in the field of r...
Conference Paper
In this work we report the application of two-dimensional gel electrophoresis (2-D electrophoresis) for the study of the quality of selected classes of biopharmaceuticals, focusing primarily on its suitability in the regulatory/pharmacopoeia environment. In order to achieve this objective, selected recombinant biological medicines, belonging to the...
Article
Full-text available
Recombinant monoclonal antibodies (rmAbs) are medicinal products obtained by rDNA technology. Consequently, like other biopharmaceuticals, they require the extensive and rigorous characterization of the quality attributes, such as identity, structural integrity, purity and stability. The aim of this work was to study the suitability of gel electrop...
Article
Full-text available
Aerial parts of Hypericum perforatum L. (Hypericaceae) were collected from five wild populations in Kosovo, with aim to investigate the chemical composition and natural variation of essential oils between wild populations. This species could be considered of economic potential as it is widespread in Kosovo, on the other hand H. perforatum is one of...
Article
Full-text available
Aerial parts of Hypericum perforatum L. (Hypericaceae) were collected from five wild populations in Kosovo, with aim to investigate the chemical composition and natural variation of essential oils between wild populations. This species could be considered of economic potential as it is widespread in Kosovo, on the other hand H. perforatum is one of...
Article
Single proteins separated by 2-DE often show multiple spots spreading along the first dimension. In many cases, such charge trains are explained by isoform differences or by putative post-translational modifications including phosphorylation, glycosylation and others. We now report that individual spots of such charge trains on 2-D gels in fact oft...
Article
The principal objective of this study was the evaluation of two-dimensional gel electrophoresis (2-DE) in combination with MALDI-TOF MS, after tryptic digest with regard to suitability for qualitative characterization and identification of therapeutic recombinant monoclonal antibodies trastuzumab and rituximab. Moreover, the impact of post-translat...
Article
Full-text available
2-DE and MALDI-TOF MS are useful techniques for the quality evaluation of medicinal products derived from recombinant DNA technology. The principal objective of this study has been to evaluate the suitability of 2-DE in combination with MALDI-TOF MS for the quality study of the therapeutic recombinant protein, abatacept. 1-DE SDS-PAGE, under reduci...
Article
Acidic macromolecules, as a nucleation factor for mollusc shell formation, are a major focus of research. It remains unclear, however, whether acidic macromolecules are present only in calcified shell organic matrices, and which acidic macromolecules are crucial for the nucleation process by binding to chitin as structural components. To clarify th...
Article
Full-text available
A simple, rapid and specific ion-pair HPLC method for the determination of (R,R)-glycopyrronium bromide and its related impurities is presented, and parameters affecting the chromatographic properties of these compounds are discussed. Optimal analyte separation was achieved on base deactivated Nucleosil at 40 degrees C, using phosphate buffer pH 2....

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Projects (2)
Project
Evaluation of a reliable 2-DE method complemented with MALDI-TOF MS analysis for identification, purity assessment and determination of charge heterogeneity/isoform pattern of biological medicinal products containing highly purified human menopausal gonadotropins (HP hMG) as active pharmaceutical ingredient. Comparison of glycan composition of these medicinal products using MALDI-TOF/TOF-MS and LCESI-MSn techniques.