Dariusz Leszczynski

University of Helsinki | HY

Electromagnetic hypersensitivity (EHS), known also as an idiopathic environmental intolerance attributed to electromagnetic fields (IEI-EMF) or a microwave sickness, is not considered by the World Health Organization (WHO) as being caused by the exposures to electromagnetic fields (EMF). EHS is not recognized as a disease anywhere in the world. Som...

While Dieudonné has praised thoroughness of Leszczynski’s review of EHS studies, he was critical of the final conclusions. Leszczynski strongly disagrees with argumentation of Dieudonné that EHS issue is settled and that biomarker research is unnecessary because it is expensive and might produce false positives. Leszczynski’s opinion is that his re...

Part of the population considers themselves as sensitive to the man-made electromagnetic radiation (EMF) emitted by powerlines, electric wiring, electric home appliance and the wireless communication devices and networks. Sensitivity is characterized by a broad variety of non-specific symptoms that the sensitive people claim to experience when expo...

The currently ongoing deployment if the fifth generation of the wireless communication technology, the 5G technology, has reignited the health debate around the new kind of radiation that will be used/emitted by the 5G devices and networks – the millimeter-waves. The new aspect of the 5G technology, that is of concern to some of the future users, i...

https://theconversation.com/do-mobile-phones-give-you-brain-cancer-63553

in press

People are continuously exposed to radiation emitted by the natural and man-made sources. Exposures to ionizing and to non-ionizing radiation are known to impact human health. Effects exerted by any type of radiation might be potentiated by co-exposures to other types of radiation as well as by co-exposures to chemicals and other environmental poll...

Acute biological effects caused by the exposure to high doses of radiation, either ionizing or non-ionizing, are relatively well known but the delayed effects, occurring decades after exposure, are difficult to predict. The knowledge of the acute and delayed effects of the low doses of ionizing radiation (e.g. bystander effect) or non-ionizing radi...

Epidemiological studies have shown that moderate and low radiation doses to the heart area may result in an increase in cardiovascular mortality. It has been suggested that cardiovascular endothelium could be one of the targets for the ionising radiation-induced heart injury. We have used human endothelial cells as an in vitro model to study immedi...

http://www.the-scientist.com/?articles.view/articleNo/35114/title/Opinion--Unethical-Reporting/

http://monographs.iarc.fr/ENG/Monographs/vol102/mono102.pdf

http://www.the-scientist.com/?articles.view/articleNo/34518/title/Opinion--Scientific-Peer-Review-in-Crisis/

Proteomics, the science that examines the repertoire of proteins present in an organism using both high-throughput and low-throughput techniques, might give a better understanding of the functional processes ongoing in cells than genomics or transcriptomics, because proteins are the molecules that directly regulate physiological processes. Not all...

Proteomics is widely used in search of biomarkers, pharmacology, clinical research and toxicology. With the help of proteomics large amount of information about the physiology of living cells can be obtained in a single experiment. Combining this information with data from genomics and other high-throughput screening techniques like transcriptomics...

High doses of ionising radiation significantly increase the risk of cardiovascular disease (CVD), the vascular endothelium representing one of the main targets. Whether radiation doses lower than 500 mGy induce cardiovascular damage is controversial. The aim of this study was to investigate radiation-induced expression changes on protein and microR...

The potential health hazard of exposure to electromagnetic fields (EMF) continues to cause public concern. However, the possibility of biological and health effects of exposure to EMF remains controversial and their biophysical mechanisms are unknown. In the present study, we used Saccharomyces cerevisiae to identify genes responding to extremely l...

We have previously shown in vitro that UVA increases the adhesiveness of mouse B16-F1 melanoma cells to endothelium.We have also shown in vivo that UVA exposure of C57BL/6 mice, i.v. injected with B16-F1 cells, increases formation of pulmonary colonies of melanoma. The aim of the present animal study was to confirm the previously observed in vivo U...

High doses of ionising radiation damage the heart by an as yet unknown mechanism. A concern for radiological protection is the recent epidemiological data indicating that doses as low as 100-500 mGy may induce cardiac damage. The aim of this study was to identify potential molecular targets and/or mechanisms involved in the pathogenesis of low-dose...

Mobile phone usage currently exceeds landline communication in Africa. The extent of this usage has raised concerns about the long-term health effects of the ongoing use of mobile phones. To assess the physiological effects of radiation from mobile phones in vitro, MCF-7 breast adenocarcinoma cells were exposed to 2W/kg non-thermal 900-MHz mobile p...

Use of mobile phones has widely increased over the past decade. However, in spite of the extensive research, the question of potential health effects of the mobile phone radiation remains unanswered. We have earlier proposed, and applied, proteomics as a tool to study biological effects of the mobile phone radiation, using as a model human endothel...

Abstract Recent reports suggest that mobile phone radiation may diminish male fertility. However, the effects of this radiation on human spermatozoa are largely unknown. The present study examined effects of the radiation on induction of apoptosis-related properties in human spermatozoa. Ejaculated, density-purified, highly motile human spermatozoa...

Biological systems are complex, variable and to a great extent adaptive to environmental and occupational challenge such as ionising radiation, making the mathematical modelling of their behaviour a diYcult task. The required models need to be based on useful experimental data describing global eVects on a cellular, tissue and organ level. High-thr...

Several recent studies have indicated that radiofrequency electromagnetic fields (RF-EMF) have an adverse effect on human sperm quality, which could translate into an effect on fertilization potential. This study evaluated the effect of RF-EMF on sperm-specific characteristics to assess the fertilizing competence of sperm. Highly motile human sperm...

There is ongoing discussion whether the mobile phone radiation causes any health effects. The International Commission on Non-Ionizing Radiation Protection, the International Committee on Electromagnetic Safety and the World Health Organization are assuring that there is no proven health risk and that the present safety limits protect all mobile ph...

INTRODUCTION There are concerns among the general population about the possibility of health hazards in response to exposure of the radiofrequency electromagnetic fields (RF-EMF) emitted from the mobile phones and their base stations. There is limited and inconclusive evidence from the epidemiological studies that the long-term (>10 years) exposure...

Background: We have earlier shown that exposure of human endothelial cell line EA.hy926 to 900 MHz GSM mobile phone radiation causes changes in the expression of numerous proteins. Here, we have examined the effects of 1800 MHz GSM mobile phone signal on the proteomeof the same cell line. Results: EA.hy926 cells were exposed for one hour to 1800 MH...

Early molecular events in radiation carcinogenesis in vivo are difficult to study, especially because it is usually impossible to know in advance the exact location of a radiation-induced tumour. In the present study, we have attempted to overcome this difficulty by exposing a very small area of hairless mouse skin to high-dose beta radiation (i.e....

Ejaculated, density purified, human spermatozoa were exposed to pulsed 900 MHz GSM mobile phone radiation at two specific absorption rate levels (SAR 2.0 and 5.7 W/kg) and compared with controls over time. Change in sperm mitochondrial membrane potential was analysed using flow cytometry. Sperm motility was determined by computer assisted sperm ana...

Earlier we have shown that the mobile phone radiation (radiofrequency modulated electromagnetic fields; RF-EMF) alters protein expression in human endothelial cell line. This does not mean that similar response will take place in human body exposed to this radiation. Therefore, in this pilot human volunteer study, using proteomics approach, we have...

Recent data suggest that there might be a subtle thermal explanation for the apparent induction by radiofrequency (RF) radiation of transgene expression from a small heat-shock protein (hsp16-1) promoter in the nematode, Caenorhabditis elegans. The RF fields used in the C. elegans study were much weaker (SAR 5-40 mW kg(-1)) than those routinely tes...

We have previously shown that ultraviolet-A (UVA) radiation enhances metastatic lung colonization capacity of B16-F1 melanoma cells. The aim of this study was to examine changes in expression profile of genes in mouse melanoma B16-F1 cells exposed to UVA radiation. B16-F1 melanoma cells were exposed to a single UVA radiation dose of 8 J/cm2 and mRN...

The applicability of high-throughput screening techniques of transcriptomics, proteomics and metabolomics in the search for biological and health effects of electromagnetic fields is a hotly debated issue. On the one hand, use of these modern screening technologies speeds up the discovery process and gives broader insight into biochemical events th...

Possible biological effects of mobile phone microwaves were investigated in vitro. In this study, which was part of the 5FP EU project REFLEX (Risk Evaluation of Potential Environmental Hazards From Low-Energy Electromagnetic Field Exposure Using Sensitive in vitro Methods), six human cell types, immortalized cell lines and primary cells, were expo...

We have examined in vitro cell response to mobile phone radiation (900 MHz GSM signal) using two variants of human endothelial cell line: EA.hy926 and EA.hy926v1. Gene expression changes were examined in three experiments using cDNA Expression Arrays and protein expression changes were examined in ten experiments using 2-DE and PDQuest software. Ob...

We have examined whether ultraviolet A (UVA) irradiation could alter adhesive properties of melanoma cells. As an experimental in vitro model, we have used C57BL/6 mouse-derived B16- F1 and B16-F10 melanoma cell lines and the syngeneic MS-1 endothelial cell line. The melanoma cells were exposed to different doses of UVA irradiation. We have determi...

The major sources of long-wave ultraviolet A radiation (UVA; 320-400 nm) exposure are extensive sunbathing and tanning in solaria. While the carcinogenic effects of mid-wave ultraviolet B radiation (UVB; 280-320 nm) are well recognized, the potentially hazardous effects of UVA are less understood. Several studies have shown that a variety of physio...

Three talks were presented in the session on "Cellular, Animal and Epidemiological Studies of the Effects of Static Magnetic Fields Relevant to Human Health". The first talk presented the in vitro effects of static magnetic fields on cell cultures. The second talk presented the in vivo evidence obtained from animal studies. The final, third talk, p...

The bystander effect is a phenomenon whereby biological consequences of irradiation are expressed in nonexposed cells in the vicinity of exposed cells. Two main pathways have been proposed to mediate the bystander effect: Gap Junction Intercellular Communication (GJIC) and medium borne soluble factors dependent mechanisms. The present study was des...

The human endothelial cell line EA.hy926 was exposed to mobile phone radiation and the effect on protein expression was examined using two-dimensional electrophoresis (2-DE). Up to 38 various proteins have statistically significantly altered their expression levels following the irradiation. Four proteins were identified with matrix-assisted laser...

We argue that the use of high-throughput screening techniques, although expensive and laborious, is justified and necessary in studies that examine biological effects of mobile phone radiation. The "case of hsp27 protein" presented here suggests that even proteins with only modestly altered (by exposure to mobile phone radiation) expression and act...

We have examined whether non-thermal exposures of cultures of the human endothelial cell line EA.hy926 to 900 MHz GSM mobile phone microwave radiation could activate stress response. Results obtained demonstrate that 1-hour non-thermal exposure of EA.hy926 cells changes the phosphorylation status of numerous, yet largely unidentified, proteins. One...

We describe a new method of cell destruction that may have potential for use in antitumor therapy. Cells are loaded by phagocytosis with microparticles (<1 microm) and irradiated with short laser pulses. Absorption of laser energy by the microparticles causes localized vaporization of the fluid surrounding the microparticles, leading to the generat...

In human coronary atheromas, the numbers of degranulated mast cells and of apoptotic smooth muscle cells (SMCs) are increased. Accordingly, the possibility exists that mast cells participate in the regulation of SMC apoptosis in the lesions. Mast cells isolated from the serosal cavities of rats were stimulated to release their secretory granules. T...

Despite years of research, the question of whether exposure to radiofrequency-modulated electromagnetic fields (RF-EMF) generated by mobile phones affects human health remains unsolved. We obtained a comprehensive overview of the possible extent of cellular response to RF-EMF irradiation by determining the total cellular changes in protein expressi...

Photodynamic therapy (PDT) generates free radicals through the absorption of light by photosensitizers. PDT shows promise in the treatment of intimal hyperplasia, which contributes to restenosis, by completely eradicating cells in the vessel wall. This study investigates the mechanisms of PDT-induced cell death. PDT, using the photosensitizer chlor...

Photodynamic therapy (PDT) inhibits experimental intimal hyperplasia. PDT results in complete vascular wall cell eradication with subsequent adventitia but minimal media repopulation. This study was designed to test the hypothesis that PDT alters the vascular wall matrix thereby inhibiting invasive cell migration, and as such, provides an important...

gamma-Irradiation (gamma-RT) and photodynamic therapy (PDT) are known to inhibit intimal hyperplasia. The common mechanism is that both modalities produce free radicals, but unlike gamma-RT, PDT generates them through the absorption of light by photosensitizers. The purpose of this in vitro study was to assess the differences that PDT and gamma-RT...

Expression of p53, K-ras, and proliferating cell nuclear antigen (PCNA) and mutations of p53 and K-ras genes in lung lesions of Han/Wistar rats were investigated by immunohistochemistry and direct DNA sequencing following a long-term exposure of animals to neutron-activated UO2 particles. The p53 protein was overexpressed in all five malignant tumo...

Die Entwicklung hämodynamisch signifikanter Gefäßstenosen nach Operation oder Angioplastie durch Intimahyperplasie und „Constrictive Remodeling“stellt ein wesentliches Hindernis für befriedigende Langzeitfunktionsraten dar. Intimahyperplasie ist definiert als Migration und Proliferation glatter Muskelzellen und Myofibroblasten in den subintimalen R...

Abstract— We examined the effects of broadband UVA radiation (320–400 nm) on a rat myeloid leukemia cell line–chlo-roma (ChL). A Phillips face tanner model HB 171/A was used as a light source. Chloroma were irradiated through a 5 mm thick glass Alter that cut off all of the UVB contamination. The irradiances were measured, from 250 to 400 nm, with...

In a previous study (Am. J. Pathol. 1994, 145: 1265-1270) we found rat coronary vascular smooth muscle cell (SMC) proliferation and apoptosis to be regulated by protein kinase C (PKC). In the present study we analysed whether selective depletion of alpha isozyme of PKC would affect SMC proliferation and/or apoptosis. First, using Western blot techn...

Protein kinase C is one of the known regulators of cellular apoptosis. However, a number of contradictory reports have been published on this topic. It has been suggested that inhibition/activation of protein kinase C can both induce and suppress apoptosis. This brief overview summarizes some of the published controversial evidence concerning prote...

We have previously demonstrated that somatostatin-14 and its octapeptide analogue, angiopeptin, decrease the ability of rat heart endothelial cells to bind leukocytes [Leszczynski, et al., Reg. Pept. 43 (1993) 131-140]. Here, we examined whether exposure of leukocytes to angiopeptin modifies their adhesiveness to the unstimulated and to IL-1 beta-a...

We examined the effect of long-wave ultraviolet radiation (UVA) on protein kinase C (PKC) and on the proliferation of rat myeloid leukemia cell line (ChL). Exposure of cells to a single dose of UVA (8 J/cm2 at 372 +/- 10 nm) caused a rapid increase in the quantity of the membrane-bound PKC, as assessed by 3H-phorbol ester (3H-PMA) binding assay (pe...

The role of protein kinase C (PKC) in regulation of the cell cycle and induction of apoptosis was examined in a rat myeloid leukemia cell line--chloroma. Exposure of chloroma cells to calphostin C (a specific PKC inhibitor) led to inhibition of cell proliferation, caused by (i) partial and transient arrest of cells in the G1 phase of the cell cycle...

We examined the effect of several inhibitors/activators of various protein kinases on the proliferation and apoptosis of nontransformed rat coronary vascular smooth muscle cells (SMC). As expected, all the compounds (calphostin C, KT5720, KT5823, verapamil, W7, and dibutyryl-cAMP) inhibited SMC proliferation, as judged by [3H]thymidine incorporatio...

It is well known that the exposure of endothelial cells to IL-1 beta induces an increase in endothelial cell adhesiveness for leucocytes. Using rat heart endothelial cells we found that exposure of endothelial cells to IL-1 beta (100 U/ml) induces a 133-fold increase in the intracellular concentration of cyclic-GMP; from 11.5 +/- 0.2 fM to 1530 +/-...

It is well known that the exposure of endothelial cells to IL-1β induces an increase in endothelial cell adhesiveness for leucocytes. Using rat heart endothelial cells we found that exposure of endothelial cells to IL-1β (100U/ml) induces a 133-fold increase in the intracellular concentration of cyclic-GMP; from 11.5×0.2fm to 1530 × 117.8 fm (per 1...

The effect of angiopeptin, a stable analogue of somatostatin, was studied on basal and interleukin-1-beta-induced endothelial cell adhesiveness for mononuclear cells, and compared to the effect of somatostatin. Angiopeptin and somatostatin decreased basal and interleukin-1-beta-induced endothelial cell adhesiveness for mononuclear cells. The decrea...

Cyclosporin A (CsA) has been suggested to potentiate graft vascular disease by stimulation of smooth muscle cell (SMC) proliferation. Both the in vitro and in vivo data are discordant, showing both stimulatory and inhibitory effects of CsA on vascular SMC proliferation. The direct and endothelial cell-mediated effects of CsA on vascular SMC prolife...

We demonstrate that rat heart coronary artery smooth muscle cells express specific binding sites (receptors) for somatostatin-14. The sigmoidal shape kinetics of the somatostatin-14 binding by the cells suggests the presence of either an allosteric binding site or binding sites with different affinities towards the ligand. Scatchard analysis reveal...

We have examined (1) the frequency of B cells secreting antibodies against donor major histocompatibility complex (MHC) antigens and (2) the properties of Thy-1-antigen-expressing leukocytes in rats rejecting renal allografts. Our results show that B cells secreting antibodies are present in the inflammatory cell population at the frequency of 1:85...

We have estimated the frequency of B cells secreting antibodies against donor MHC antigens in rats rejecting histoincompatible renal allografts. In a major plus minor antigen-incompatible DA-to-WF combination on day 4 post-transplantation, reverse protein A plaque assay demonstrated that in the graft the frequency of lymphoid cells secreting Ig was...

We have investigated if recombinant granulocyte-macrophage colony stimulating factor (GM-CSF), alone or in concert with recombinant gamma interferon, affects the endothelial cell expression of class I major histocompatibility complex antigen. Results obtained show that the GM-CSF increases class I expression on the endothelial cell in a time- and d...

Prostacyclin is generated by cultured rat endothelial cells. Compound blocking activity of protein kinase C and cyclic nucleotide-dependent protein kinases (H7) and compound blocking interaction between Ca2+ and calmodulin (W7) diminish generation of prostacyclin in rat endothelial cells. These compounds give a synergistic effect when they are intr...

Modulation of the major histocompatibility (MHC) antigen expression on rat endothelial cells by a mixture of cytokines has been examined. Experiments were performed employing both enzyme-linked immunoassay (ELISA) and fluorescence-activated cell-sorting (FACS) techniques and recombinant cytokines: interleukin-1 alpha (IL-1 alpha), tumor necrosis fa...

It appears that allograft rejections can be considered as a series of cellular and molecular cascades of inflammation triggered by the immune response. In acute rejection, inflammatory changes with a prominent blast cell component dominate. The main result is toxic damage of several parenchymal components, particularly the microvascular endothelium...

When the endothelial cells (ECs) were stimulated with gamma-interferon (gIFN) in the presence of methylprednisolone (MP) or prostaglandin E2 (PGE2), MP enhanced gIFN-induced Ia antigen expression, whereas PGE2 inhibited it. On the other hand, while PGE2 had no effect on leucocyte binding to ECs, MP entirely inhibited it. By using selective inhibito...

Leukotrienes are potent mediators of local microvascular environment. Leukotriene B4 treatment of cultured endothelium increases the binding of lymphocytes to endothelial cell monolayers within minutes. This effect is dose-dependent and reversible upon removal of the leukotriene. Pretreatment of lymphocytes slightly decreases the binding and pretre...

The purpose of this communication is to reconsider the role of CTL in acute allograft rejection, compare the frequency of donor-specific components to other inflammatory components in situ, and place the donor-specific CTL in proper perspective in the rejection process.

We have investigated the reasons why thymectomized, bone marrow-reconstituted (B) rats do not reject their allografts, by comparing the structure of inflammation and functions of inflammatory cells in nonrejecting allografts to rejecting allografts in normal control recipients. The results demonstrate that B recipients mount a specific cellular res...

We have investigated the immunogenic potential of rat heart vascular endothelial cells by their ability to induce an accelerated rejection of a relevant heart allograft, and related the immunogenic potential to the expression of class II major histocompatibility complex (MHC) antigens on the endothelial cell surface. Only 12% of freshly isolated ra...