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Dario Monachello

Dario Monachello
IPS2-INRA

PhD

About

22
Publications
4,416
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2,219
Citations
Introduction
The OGE team fully automated and improved a liquid Y2H protocol. Using 384-well plates, successive culture replication in liquid media and robots, the InterATOME Platform is able to screen a pool of 50 DB-X hybrid proteins against the AIM AD-library (about 12000 Arabidopsis proteins) within two months.
Additional affiliations
December 2013 - January 2015
Genopole
Position
  • PostDoc Position
February 2011 - November 2013
French National Centre for Scientific Research
Position
  • Engineer
November 2008 - January 2010
Dana-Farber Cancer Institute
Position
  • PostDoc Position

Publications

Publications (22)
Article
Full-text available
The majority of the pyruvate inside plant mitochondria is either transported into the matrix from the cytosol via the mitochondria pyruvate carrier (MPC) or synthesized in the matrix by alanine aminotransferase (AlaAT) or NAD-malic enzyme (NAD-ME). Pyruvate from these origins could mix into a single pool in the matrix and contribute indistinguishab...
Article
Full-text available
Mobile group II introns are site-specific retrotransposable elements abundant in bacterial and organellar genomes. They are composed of a large and highly structured ribozyme and an intron-encoded reverse transcriptase that binds tightly to its intron to yield a ribonucleoprotein (RNP) particle. During the first stage of the mobility pathway, the i...
Preprint
Full-text available
The majority of the pyruvate inside plant mitochondria is either transported into the matrix from the cytosol via the mitochondria pyruvate carrier (MPC) or synthesised in the matrix by alanine aminotransferase (AlaAT) or NAD-malic enzyme (NAD-ME). Pyruvate from these origins could mix into a single pool in the matrix and contribute indistinguishab...
Article
Full-text available
Pathogens deploy effector proteins that interact with host proteins to manipulate the host physiology to the pathogen's own benefit. However, effectors can also be recognized by host immune proteins, leading to the activation of defence responses. Effectors are thus essential components in determining the outcome of plant–pathogen interactions. Des...
Article
The lack of resolution on the many different ubiquitin chain types found in eukaryotic cells has been a major hurdle to our understanding of their specific roles. We currently have very little insight into the cellular and physiological functions of lysine(K)63-linked ubiquitin chains, although they are the second most abundant forms of ubiquitin i...
Preprint
Full-text available
Pathogens deploy effector proteins that interact with host proteins to manipulate the host physiology to the pathogen’s own benefit. However, effectors can also be recognized by host immune proteins leading to the activation of defense responses. Effectors are thus essential components in determining the outcome of plant-pathogen interactions. Desp...
Article
Full-text available
Physical interactions mediated by proteins are a critical element of biological systems, and the analysis of interaction partners can provide valuable hints about unknown functions of a protein. Two major classes of experimental approaches are used for protein interaction mapping: analysis of direct interactions using binary methods such as yeast t...
Article
Full-text available
The 2′-5′ branch of nuclear premessenger introns is believed to have been inherited from self-splicing group II introns, which are retrotransposons of bacterial origin. Our crystal structures at 3.4 and 3.5 angstrom of an excised group II intron in branched (“lariat”) form show that the 2′-5′ branch organizes a network of active-site tertiary inter...
Article
Full-text available
When assayed in vitro, group IIC self-splicing introns, which target bacterial Rho-independent transcription terminators, generally fail to yield branched products during splicing despite their possessing a seemingly normal branchpoint. Starting with intron O.i.I1 from Oceanobacillus iheyensis, whose crystallographically determined structure lacks...
Article
In recent years RNA molecules have emerged as central players in the regulation of gene expression. Many of these noncoding RNAs possess well-defined, complex, three-dimensional structures which are essential for their biological function. In this context, much effort has been devoted to develop computational and experimental techniques for RNA str...
Article
Full-text available
In mammals, the Voltage-dependent anion channels (VDACs) are predominant proteins of the outer mitochondrial membrane (OMM) where they contribute to the exchange of small metabolites essential for respiration. They were shown to be as well associated with the plasma membrane (PM) and act as redox enzyme or are involved in ATP release for example. I...
Article
Full-text available
Plants have unique features that evolved in response to their environments and ecosystems. A full account of the complex cellular networks that underlie plant-specific functions is still missing. We describe a proteome-wide binary protein-protein interaction map for the interactome network of the plant Arabidopsis thaliana containing about 6200 hig...
Article
Full-text available
Plants generate effective responses to infection by recognizing both conserved and variable pathogen-encoded molecules. Pathogens deploy virulence effector proteins into host cells, where they interact physically with host proteins to modulate defense. We generated an interaction network of plant-pathogen effectors from two pathogens spanning the e...
Article
Physical interactions mediated by proteins are critical for most cellular functions and altogether form a complex macromolecular "interactome" network. Systematic mapping of protein-protein, protein-DNA, protein-RNA, and protein-metabolite interactions at the scale of the whole proteome can advance understanding of interactome networks with applica...
Article
Full-text available
* In plants, the knowledge of the molecular identity and functions of anion channels are still very limited, and are almost restricted to the large ChLoride Channel (CLC) family. In Arabidopsis thaliana, some genetic evidence has suggested a role for certain AtCLC protein members in the control of plant nitrate levels. In this context, AtClCa has b...
Article
Full-text available
Plants need nitrate for growth and store the major part of it in the central vacuole of cells from root and shoot tissues. Based on few studies on the two model plants Arabidopsis thaliana and rice, members of the large ChLoride Channel (CLC) family have been proposed to encode anion channels/transporters involved in nitrate homeostasis. Proteins f...
Article
Full-text available
Though numerous pieces of evidence point to major physiological roles for anion channels in plants, progress in the understanding of their biological functions is limited by the small number of genes identified so far. Seven chloride channel (CLC) members could be identified in the Arabidopsis genome, amongst which AtCLCe and AtCLCf are both more c...
Article
Full-text available
Nitrate, the major nitrogen source for most plants, is widely used as a fertilizer and as a result has become a predominant freshwater pollutant. Plants need nitrate for growth and store most of it in the central vacuole. Some members of the chloride channel (CLC) protein family, such as the torpedo-fish ClC-0 and mammalian ClC-1, are anion channel...
Article
Full-text available
In the present study we emphasized the differences between tomato (Lycopersicon esculentum cv. Mill) leaves cytosolic (cytosolic nitrate reductase, cNR) and membrane-bound nitrate reductase (mNR) by comparing their activity during prolonged darkness, under salt stress conditions as well as their response to nitrate induction. Tomato leaves mNR rema...

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Project (1)
Project
The IPS2 InterATOME platform offer an open service for Y2H screening of Arabidopsis protein interactions: ORF clones from our collaborators are screened against individual and ordered clones corresponding to nearly half of the Arabidopsis proteins. Working with 384-well plates and robots the InterATOME Platform is able to screen up to 50 DB-X hybrid proteins against the AD-library (about 12000 Arabidopsis proteins).