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51
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Introduction
Skills and Expertise
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January 2004 - present
Position
- Profesora Titular, Investigadora,
Description
- Desarrollo de protocolos para la propagación de plantas leñosas, estudios, hidtomorfológicos, fisiológicos, moleculares de la propagación vegetativa (enraizamiento, orgagogénesis, embriogénesis somática
Publications
Publications (51)
From in vitro cultured adult material of Corylus avellana L. cv. Negretta, adventitious rooting of microshoots was evaluated. Rhizogenic induction mediated by two strains of wild-type Agrobacterium rhizogenes (A477 and A478) and indolbutyric acid (IBA) were compared under two light conditions (16:8 h photoperiod and complete darkness). The results...
Castanea sativa Mill. is a specie of agroforestry interest widely grown in Europe, its current production it is mainly focused on the extraction of a quality brown fruit, highly demanded in the gourmet industry. In Chile, this specie might be successfully grown due to the climate and lack of diseases that may affect it. However, there is not a prop...
In forest production systems, vegetative propagation of elite clones through adventi-tious rooting is a common practice. In Chile, adventitious rooting is the main methodology for vegetative reproduction of Pinus radiata. However, the capability of produce adventitious roots in gymnosperms decreases with aging. While it is true that some efforts ha...
A major problem in forest clonal productivity is the loss of morphogenetic capability with the increasing age of plants. However, despite of the importance of loss of morphogenetic competence, very little research has been done about the underlying mechanisms involved in this process. For this reason, a gene expression analysis using dot blot techn...
In forest production systems, vegetative propagation of elite clones through adventi-tious rooting is a common practice. In Chile, adventitious rooting is the main methodology for vegetative reproduction of Pinus radiata. However, the capability of produce adventitious roots in gymnosperms decreases with aging. While it is true that some efforts ha...
The aim of the present study was to evaluate the applicability of maximal photochemical efficiency of photosystem II (Fv/Fm) as an early estimate of P. radiata micrografts viability coming from dif-ferent position (basal vs. apical) in the ortets. We hypothesize that Fv/Fm variation is a good indi-cator of micrograft's viability and phenological st...
We study, how in vitro light and ventilation affect physiological performance and survival of ex vitro Castanea sativa plantlets. In vitro treatments consisted of two irradiances of 50 and 150 µmol m-2 s-1 in ventilated vessels (VL50 and VL150, respectively), compared to traditional cultures at 50 µmol m-2 s-1 in non-ventilated vessels (NVL50). Aft...
Induction of somatic embryos of Eucalyptus globulus from seeds, hypocotyls, cotyledons and micropropagated leaves was studied. The material with the highest proliferation rate was transferred to differentiation treatments. The best treatments were determined based on growth, oxidation and embryogenic development stages. Finally, the somatic cotyled...
The limited development of photoprotective mechanisms, specifically heat dissipation capacity, found in micropropagated plants may be the result of low xanthophyll cycle pigment content and reduced de-epoxidation capacity making them highly susceptible to photodamage. The effects of gradual or sudden increase of light on Castanea sativa in vitro cu...
The effects of increased photon flux (100 lmol m -2 s -1), ventilation, and standard in vitro cul-ture (40 lmol m -2 s -1) with no ventilation were investi-gated on the physiological and histological characteristics of microshoots of Gevuina avellana. The increase in pho-ton flux (light treatment) produced significant improvement in the fluorescenc...
The effects of increased photon flux (100 lmol m -2 s -1), ventilation, and standard in vitro cul-ture (40 lmol m -2 s -1) with no ventilation were investi-gated on the physiological and histological characteristics of microshoots of Gevuina avellana. The increase in pho-ton flux (light treatment) produced significant improvement in the fluorescenc...
a b s t r a c t The effects of in vitro environmental conditions, ventilation of culture vessels and light level, on water loss control and photosynthetic capacity of Castanea sativa during in vitro culture were assessed. C. sativa microshoots were cultured in ventilated (V) and non ventilated (NV) vessels, using two photon flux den-sity (PFD) leve...
a b s t r a c t The effects of in vitro environmental conditions, ventilation of culture vessels and light level, on water loss control and photosynthetic capacity of Castanea sativa during in vitro culture were assessed. C. sativa microshoots were cultured in ventilated (V) and non ventilated (NV) vessels, using two photon flux den-sity (PFD) leve...
The anatomic and functional leaf characteristics related to photosynthetic performance of Castanea sativa growing in vitro and in nursery were compared. The irradiance saturated photosynthesis in in vitro grown plantlets was significantly lower compared to nursery plants (65 vs. 722 µmol m -2 s -1). The maximum photosynthetic rate (P Nmax) was 4.0...
The anatomic and functional leaf characteristics related to photosynthetic performance of Castanea sativa growing in vitro and in nursery were compared. The irradiance saturated photosynthesis in in vitro grown plantlets was significantly lower compared to nursery plants (65 vs. 722 µmol m -2 s -1). The maximum photosynthetic rate (P Nmax) was 4.0...
Phase change in higher plants, from juvenile to mature, affects the reproductive competence, morphology and growth rate, as well as the regenerative potential of tissue explants. However, in conifers these changes can be achieved before the onset of flowering and at the beginning of the seed production. Early maturational changes in conifers are ve...
This study evaluated the expression of defense compounds by the secondary metabolism of Eucalyptus globulus plants subjected to attacks by the insect Ctenarytaina eucalypti (blue gum psyllid) and abiotic mechanical damage using gas chromatography with flame ionization detection (GC-FID) and mass detection (MS). The results show that both biological...
Con el objeto de establecer metodologías de masificación de Eucalyptus globulus se analizaron factores que afectan la multiplicación in vitro de esta especie mediante el sistema de inmersión temporal. Para ello se dispusieron cinco microtallos por frasco, conteniendo 250 mL de medio Murashige y Skoog. Se estudiaron la frecuencia de inmersión (6, 12...
To define preliminary aspects of walnut (Juglans regia L.) callogenesis in indirect propagation systems, this work analyzed callogenic induction in adult leaves of walnut in broadleaf trees (BTM), Murashige and Skoog (MS) Driver Kuniyuki Walnut (DKW) and Woody Plant (WPM) media. Leaf segments of 1 cm 2 , previously sterilized, were placed in each c...
The in vitro introduction of adult walnut ( Juglans regia L.) tissue represents an opportunity to clone elite genotypes whose selection occurs in advanced ontogenic states. With the purpose of developing a protocol to allow mass propagation of valuable genotypes from adult material, a comparison was made between two root induction systems of walnut...
From in vitro cultured adult material of Corylus avellana L. cv. Negretta, adventitious rooting of microshoots was evaluated. Rhizogenic induction mediated by two strains of wild-type Agrobacterium rhizogenes (A477 and A478) and indolbutyric acid (IBA) were compared under two light conditions (16:8 h photoperiod and complete darkness). The results...
From in vitro cultured adult material of Corylus avellana L. cv. Negretta, adventitious rooting of microshoots was evaluated. Rhizogenic induction mediated by two strains of wild-type Agrobacterium rhizogenes (A477 and A478) and indolbutyric acid (IBA) were compared under two light conditions (16:8 h photoperiod and complete darkness). The results...
Somatic and zygotic embryos from mature seeds of rauli-beech, Nothofagus alpina (Poepp. & Endl.) Oerst., were encapsulated in different artificial endosperms in order to generate a cover that fulfills the function of nourishment and protection of the embryos, facilitating their later germination. The content of sodium alginate varied by 4%, 3%, and...
Quantification of deoxynucleosides using micellar high-performance capillary electrophoresis (HPCE) is an efficient, fast and inexpensive evaluation method of genomic DNA methylation. This approach has been demonstrated to be more sensitive and specific than other methods for the quantification of DNA methylation content. However, effective detecti...
SUMMARY The effect of two asepsis protocols, scion type (apical shoot tips and brachyblast), gathering season (autumn and summer) and inser- tion position of the scion in the ortet (apical and basal) upon the micrograft establishment and consolidation of Pinus radiata D. Don. was evaluated. Scions were obtained from mature nursery cultivated clones...
The effect of two asepsis protocols, scion type (apical shoot tips and brachyblast), gathering season (autumn and summer) and insertion position of the scion in the ortet (apical and basal) upon the micrograft establishment and consolidation of Pinus radiata D. Don. was evaluated. Scions were obtained from mature nursery cultivated clones. The asep...
Se estudió el efecto de subcultivos sucesivos sobre la producción de microtallos desde líneas organogénicas de material cigótico de Eucalyptus globulus. Cada 50 días fueron subcultivados segmentos nodales en medio de proliferación (MS + 4,4 µM BAP + 0,05 µM ANA) y se contabilizó el número de microtallos por explanto. Los resultados indicaron una di...
Se estudió la inducción de callo embriogénico a partir de tres explantos provenientes de semillas maduras (embriones cigóticos maduros, hipocótilos y cotiledones). Los resultados indicaron que el medio I3 (B5 + ANA 0,5mg·l-1 + BAP 1,0mg·l-1) produjo los mayores porcentajes de formación de callo en todos los explantes. La micromorfología evidenció p...
Se estudió la inducción caulogénica utilizando semillas maduras como explantos iniciales. Para la callogénesis se evaluaron cuatro concentraciones de BAP (0, 3, 5 y 7 mg L1) en los medios MS, B5 y SP. Para la caulogénesis se utilizaron los callos formados en la etapa anterior, evaluando el efecto de dos concentraciones de ANA (0,1 y 0,5 mg L1), m...
Chestnut in vitro morphogenic competence was analyzed in shoot segments from the same adult trees but taken from branches with different ontogenetic development, juvenile-like and mature. During the establishment and the proliferation phases differences were quantified according to the ontogenic nature of the explants. Despite the establishment pha...
Este estudio tuvo por objeto determinar el número de subcultivo y el diámetro basal en el cual se obtiene la mayor tasa de enraizamiento ex vitro para microtallos de castaño, Castanea sativa Mill., obtenidos vía cultivo in vitro. El material vegetal correspondió a microtallos provenientes del cultivo de embriones con entre 7 y 12 subcultivos durant...
En este libro, junto con los alcances técnicos de las temáticas relacionadas con la clonación
de raulí, se analizan también los aspectos legales y las consideraciones fundamentales de la
silvicultura clonal y los ensayos clonales. También se incluyen las tecnologías tradicionales
de propagación vegetativa y se analizan las consideraciones y perspec...
Se analizó la duración del efecto revigorizante de podas severas de avellano europeo (Corylus avellana L.) cv. Negretta adulto. Se evaluó la estimulación y la tasa de proliferación in vitro, sobre explantos resultantes de la estimulación de varetas obtenidas desde plantas adultas sin poda (T1), brotes epicórmicos de plantas sin poda (T2), plantas p...
In vitro propagation of Nothofagus procera was studied in MS and BTM culture media using mature embryos as the initiation material. Even at the embrionary establishment phase, there was no statistical difference between two media and the MS medium was selected because it produced more vigorous microseedlings. In the following proliferation phase, t...
The duration of the reinvigorating effect of severe pruning on mature European hazelnuts ( Corylus avellana L.) cv. Negretta was analyzed. The in vitro stimulation and proliferation rate of the resulting explants from cutting stimulation obtained from adult plants not pruned (T1), epicormic shoots of non-pruned plants (T2), once-pruned plants (T3)...
Eucalyptus globulus Labill. ssp. globulus seedlings produced in containers under homogeneous morphological conditions were treated with short photoperiods of 8 hours for 7 and 15 days. The effect was compared with that of a normal photoperiod of 10 hours in a nursery (treatment control), and evaluated using three methods: the relative electrolytic...
To characterize the length of the induction, initiation and manifestation phases of the walnut rooting process, two types of explants have been used: cotyledonar linking areas (CLA) and cotyledonary portions (CP), which are referred as spontaneous and inductible rooting test systems, respectively. Both types of explants were cultured in basal mediu...
Histochemical specific stains, have revealed that parenchymatic cells of cotyledonary portions are full of starch granules, lipids and protein bodies. Through the rooting process, some parenchymatic cells became elongated and after 5 days of culture, they appeared nearly empty of storage materials. These type of cells were surrounding the other sma...
Former studies determined the different rooting capacity of three explants derived from walnut seeds. One of them had been defined as spontaneous rooting system (cotyledonar linking areas, CLA) and the others two as inducible rooting systems (cotyledonary portions, CP and embryonic microshoots, EM). In this work we try to stablish some of physiolog...
Changes in composition of various lipid fractions of leaves of the woody evergreen Nothofagus dombeyi hardened either under natural (at high and low altitude) or under laboratory conditions were determined. Frost hardiness of the leaves reached a maximum in the cold period (LT5O) - 5° in the lowest place and - 10.2° in the highest site and was asso...
The annual course of frost hardiness and soluble carbohydrate content in leaves of woody Nothofagus species, located at different attitudes in Southern Chile, were studied. Frost resistance reached a maximum in the coldest period and a minimum in spring or summer. Leaves from individuals subjected to lower temperatures (higher altitudes) were more...
The annual course of frost resistance and free proline content was studied in leaves at different stages of development of a woody species (Nothofagus dombeyi) from Southern Chile. The freezing resistance reached a minimum in late spring or summer and a maximum in the autumn-winter period. Adult and juvenile trees showed a similar degree of resista...
A B S T R A C T The duration of the reinvigorating effect of severe pruning on mature European hazelnuts (Corylus avellana L.) cv. Negretta was analyzed. The in vitro stimulation and proliferation rate of the resulting explants from cutting stimulation obtained from adult plants not pruned (T1), epicormic shoots of non-pruned plants (T2), once-prun...
This study had the objective of determining the subculture number and the shoot basal diameter that produces the best rooting rate ex vitro of chestnut, Castanea sativa Mill., microshoots obtained via in vitro culturing. The plant material corresponded to microcuttings obtained from embryo cultures with between 7 and 12 subcultures in the prolifera...
