Dany Gaillard

Dany Gaillard
University of Colorado | UCD · Department of Cell and Developmental Biology

PhD

About

37
Publications
4,443
Reads
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743
Citations
Introduction
I have always been interested in how what we eat can regulate our metabolism, how the body regulates food intake to modulate its own metabolism and maintain homeostasis, and how that impacts our health. Taste is a fundamental sense that controls food intake. Taste bud cells are continuously renewed throughout adult life. My current research focuses on the role of Wnt/β-catenin signaling in taste cell renewal in adults, and investigates how head and neck irradiation disrupts taste cell renewal.
Additional affiliations
June 2014 - present
University of Colorado
Position
  • Instructor
Description
  • Role of Wnt/β-catenin signaling pathway in taste cell renewal
October 2009 - June 2014
University of Colorado
Position
  • PostDoc Position
Description
  • Role of Wnt/β-catenin signaling pathway in taste cell renewal
September 2005 - August 2009
INSERM U866-University of Burgundy
Position
  • On the track of « fat taste »: involvement of gustation in the oro-sensory perception of dietary lipids and role of the receptor-like protein CD36
Education
October 2005 - July 2009
September 2004 - July 2005
September 2002 - September 2004

Publications

Publications (37)
Preprint
S ummary Taste buds on the tongue are collections of taste receptor cells (TRCs) that detect sweet, sour, salty, umami and bitter stimuli. Like non-taste lingual epithelium, TRCs are renewed from basal keratinocytes, many of which express the transcription factor SOX2. Genetic lineage tracing has shown SOX2+ lingual progenitors give rise to both ta...
Article
Full-text available
Background and aims: Hypercholesterolemia is a major risk factor for atherosclerosis and cardiovascular diseases. Although resistant to hypercholesterolemia, the mouse is a prominent model in cardiovascular research. To assess the contribution of bile acids to this protective phenotype, we explored the impact of a 2-week-long dietary cholesterol o...
Article
Full-text available
Embryonic taste bud primordia are specified as taste placodes on the tongue surface and differentiate into the first taste receptor cells (TRCs) at birth. Throughout adult life, TRCs are continually regenerated from epithelial progenitors. Sonic hedgehog (SHH) signaling regulates TRC development and renewal, repressing taste fate embryonically, but...
Article
The sense of taste is mediated by taste buds on the tongue, which are composed of rapidly renewing taste receptor cells (TRCs). This continual turnover is powered by local progenitor cells and renders taste function prone to disruption by a multitude of medical treatments, which in turn severely impacts the quality of life. Thus, studying this proc...
Article
Since the early 20 th century, progress in cancer therapies has significantly improved disease prognosis. Nonetheless, cancer treatments are often associated with side effects that can negatively affect patient wellbeing and disrupt the course of treatment. Among the main side effects, taste impairment is associated with depression, malnutrition an...
Preprint
Full-text available
Embryonic taste bud primordia are specified as taste placodes on the tongue surface and differentiate into the first taste receptor cells (TRCs) at birth. Throughout adult life, TRCs are continually regenerated from epithelial progenitors. Sonic hedgehog (SHH) signaling regulates TRC development and renewal, repressing taste fate embryonically, but...
Article
Full-text available
Head and neck cancer patients receiving conventional repeated, low dose radiotherapy (fractionated IR) suffer from taste dysfunction that can persist for months and often years after treatment. To understand the mechanisms underlying functional taste loss, we established a fractionated IR mouse model to characterize how taste buds are affected. Fol...
Article
Full-text available
Taste stimuli are transduced by taste buds and transmitted to the brain via afferent gustatory fibers. Renewal of taste receptor cells from actively dividing progenitors is finely tuned to maintain taste sensitivity throughout life. We show that conditional β-catenin deletion in mouse taste progenitors leads to rapid depletion of progenitors and Sh...
Data
Krt5-β-catenin LOF reduces the number and size of taste buds in foliate papillae (FolP) and soft palate (SP). The number and size of taste buds was significantly reduced in the FolP (A-C) and SP (D-F) of mutant mice compared to those of controls. Data are represented as scatter plots (individual symbols), and median with 1st and 3rd quartile (blue...
Data
β-catenin deletion in Krt5+ progenitors reduces all 3 cells types in FFP. (A) NTPDase2 (green) marks the membranes of Type I glial-like taste cells, as well as a subset of mesenchymal cells adjacent to the CVP epithelium (white arrowheads). In the anterior tongue, the area of NTPdase2-immunostaining (green) per FFP Krt8+ taste bud profile was reduc...
Data
β-catenin immunostaining is reduced in basal keratinocytes and taste buds in Krt5-β-catenin LOF mice. Circumvallate papillae (CVP) and fungiform papillae (FFP) were harvested from Krt5rtTA;tetOCre;Catnbflox(exon2-6) mice fed doxycycline chow for 4 weeks, and cryosections immunostained for β-catenin. Perigemmal basal cells (white arrowheads), includ...
Data
Wnt signaling pathway real-time PCR array data. (DOCX)
Data
Krt5-β-catenin LOF mice show reduced preference for saccharine and have significant weight loss compared to controls. (A) The weight of control and mutant mice was measured at 2 weeks and 7 weeks of doxycycline chow. Beta-catenin deletion in Krt5+ progenitor cells was associated with significant weight loss. Data are represented as scatter plots (i...
Article
Full-text available
Human WNT10A mutations are associated with developmental tooth abnormalities and adolescent onset of a broad range of ectodermal defects. Here we show that β-catenin pathway activity and adult epithelial progenitor proliferation are reduced in the absence of WNT10A, and identify Wnt-active self-renewing stem cells in affected tissues including hair...
Data
Supplementary figures and supplementary table.
Article
The natural like and dislike of foods based on taste is one of the most easily observed behaviors in animals. Animals eat palatable foods and reject aversive foods, which makes measurement of taste perception possible using various behavioral techniques. Three different methods to accurately measure taste behavior are described here. First, two-bot...
Article
Full-text available
Author Summary Taste is a fundamental sense that helps the body determine whether food can be ingested. Taste dysfunction can be a side effect of cancer therapies, can result from an alteration of the renewal capacities of the taste buds, and is often associated with psychological distress and malnutrition. Thus, understanding how taste cells rene...
Article
Full-text available
Recent studies in rodents and humans suggest that the chemoreception of long-chain fatty acids (LCFA) in oral cavity is involved in the spontaneous preference for fatty foods and might contribute to the obesity risk. CD36 and GPR120 are LCFA receptors identified in rodent taste bud cells. The fact that CD36 or GPR120 gene inactivation leads to a de...
Article
Wnt/β-catenin signaling initiates taste papilla development in mouse embryos, however, its involvement in taste cell turnover in adult mice has not been explored. Here we used the BATGAL reporter mouse model, which carries an engineered allele in which the LacZ gene is expressed in the presence of activated β-catenin, to determine the responsivenes...
Article
The profound technical and economic changes of the 20th Century had a deep effect on our way of life and, consequently, on our eating behaviour. For the first time in its history, much of the world's population no longer has to “run after calories”. An abundance of food has obvious consequences: it promotes our specific appetites. Lipids account fo...
Article
Obesity is recognized as a worldwide health problem. Overconsumption of fatty foods contributes significantly to this phenomenon. Rodents, like humans, display preferences for lipid-rich foods. Rodents thus provide useful models to explore the mechanisms responsible for this complex feeding behavior resulting from the integration of multiple oral a...
Article
Obesity constitutes a major public health problem for the twenty-first century, with its epidemic spread worldwide, particularly in children. The overconsumption of fatty foods greatly contributes to this phenomenon. Rodents and humans display a spontaneous preference for lipid-rich foods. However, the molecular mechanisms underlying this pattern o...
Article
Full-text available
Obesity constitutes a major public health problem for the 21st Century, with its epidemic spread worldwide, particularly in children. The overconsumption of fatty foods greatly contributes to this phenomenon. Rodents and humans display a spontaneous preference for lipid-rich foods. But why are lipids so attractive? What are the molecular mechanisms...
Article
Obesity constitutes a major public heath problem for the 21st Century, with its epidemic spread worldwide, particularly in children. The overconsumption of fatty foods greatly contributes to this phenomenon. Rodents and humans display a spontaneous preference for lipid-rich foods. But why are lipids so attractive? What are the molecular mechanisms...
Article
Full-text available
The sense of taste informs the body about the quality of ingested foods. Tastant-mediated signals are generated by a rise in free intracellular calcium levels ([Ca(2+)]i) in the taste bud cells and then are transferred to the gustatory area of brain via connections between the gustatory nerves (chorda tympani and glossopharyngeal nerves) and the nu...
Article
Origin of spontaneous fat preference in mammals remains elusive. It is clearly a multimodal system resulting from the integration of orosensory and post-ingestive signals. For a long time, dietary lipids were thought to be only sensed in the oral cavity through their textural and olfactory cues. Recent data strongly suggest that the sense of taste...
Article
Full-text available
Origin of spontaneous fat preference in mammals remains elusive. It is clearly a multimodal system resulting from the integration of orosensory and post-ingestive signals. For a long time, dietary lipids were thought to be only sensed in the oral cavity through their textural and olfactory cues. Recent data strongly suggest that the sense of taste...
Article
Sense of taste informs the body about the quality of ingested foods. Five sub-modalities allowing the perception of sweet, salty, sour, bitter, and umami stimuli are classically depicted. However, the inborn attraction of mammals for fatty foods raises the possibility of an additional orosensory modality devoted to fat perception. For a long time,...
Article
Full-text available
Faecal bile acid elimination greatly contributes to cholesterol homeostasis. Synthesised from cholesterol in the liver, bile acids are actively reclaimed in the ileum by the apical sodium dependent bile acid transporter (ASBT). Although the expression level of ASBT affects body cholesterol balance, the impact of cholesterol on ASBT gene expression...
Article
Sense of taste informs the body about the quality of ingested foods. Five sub-modalities allowing the perception of sweet, salty, sour, bitter, and umami stimuli are classically depicted in Human. However, the spontaneous attraction of mammals for fatty foods raises the possibility of an additional oro-sensory modality devoted to the fat perception...
Article
Sense of taste informs the body about the quality of ingested foods. Five sub-modalities allowing the perception of sweet, salty, sour, bitter, and umami stimuli are classically depicted in Human. However, the spontaneous attraction of mammals for fatty foods raises the possibility of an additional oro-sensory modality devoted to the fat perception...