Danny Jr. Honorio

Danny Jr. Honorio
University of the Philippines Visayas | UPV · School of Technology

Chemical Engineering

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Questions

Questions (4)
Question
What is the proper cleaning procedure for glassware used in mercury nitrate synthesis? After disposing mercury waste, I am sure that there may be some residual mercury traces left in the glassware. How can I effectively remove these contaminants? Are specialized cleaning agents necessary, or are acid washing and using surfactants and other common cleaning agents enough?
Question
Hello! I am currently doing my thesis on mercury bioremediation using bacteria. To understand the underlying mechanisms, I'm seeking your recommendations for suitable analytical techniques. I'm particularly interested in investigating processes like biodegradation, biotransformation, absorption, and adsorption. My current proposals include Scanning Electron Microscopy - Energy Dispersive X-Ray Spectroscopy (SEM-EDS) to examine bacterial-mercury interactions, Atomic Absorption Spectroscopy (AAS) to assess mercury transformation, and UV-Vis Spectrophotometry to quantify mercury removal.
Question
I have read many journals about the bioremediation of mercury using bacteria. All of them have used mercuric chloride in their experiments. However, this compound is a little tricky to synthesize. Can I use mercury (II) nitrate instead?
Question
I'm interested in studying mercury-resistant bacteria. Could you please provide guidance on safe and ethical experimental procedures, including proper disposal methods for agar with mercury and other materials?