Danielle Gruen

University of Pittsburgh | Pitt · Department of Surgery

Objectives The timing of and interval between events in prehospital care is important for system design, patient outcome, and prehospital research. Since these data can guide treatment recommendations, it is imperative that time-based prehospital documentation is accurate and precise, especially for time-sensitive conditions such as out-of-hospital...

Methane is a key component in the global carbon cycle with a wide range of anthropogenic and natural sources. Although isotopic compositions of methane have traditionally aided source identification, the abundance of its multiply-substituted "clumped" isotopologues, e.g., (13)CH3D, has recently emerged as a proxy for determining methane-formation t...

The abundance of methane isotopologues with two rare isotopes (e.g., ¹³CH3D) has been proposed as a tool to estimate the temperature at which methane is formed or thermally equilibrated. It has been shown, however, that microbial methane from surface environments and from laboratory cultures is characterized by low ¹³CH3D abundance, corresponding t...

BACKGROUND Prehospital plasma improves survival in severely injured patients transported by air ambulance. We hypothesized that prehospital plasma would be associated with a reduction in immune imbalance and endothelial damage.METHODS We sampled blood from 405 trauma patients enrolled in the Prehospital Air Medical Plasma (PAMPer) trial upon hospit...

Importance Prehospital plasma administration improves survival in injured patients at risk for hemorrhagic shock and transported by air ambulance. Traumatic brain injury (TBI) is a leading cause of death following trauma, but few early interventions improve outcomes. Objective To assess the association between prehospital plasma and survival in pa...

Alterations in lipid metabolism have the potential to be markers as well as drivers of pathobiology of acute critical illness. Here, we took advantage of the temporal precision offered by trauma as a common cause of critical illness to identify the dynamic patterns in the circulating lipidome in critically ill humans. The major findings include an...

Objectives: We sought to identify causal factors that explain the selective benefit of pre-hospital administration of thawed plasma (TP) in traumatic brain injury (TBI) patients using mediation analysis of a multi-omic database. Summary background data: The Prehospital Air Medical Plasma (PAMPer) Trial showed that patients with TBI and a pronoun...

Gas hydrates stored in the continental margins of the world’s oceans represent the largest global reservoirs of methane. Determining the source and history of methane from gas hydrate deposits informs the viability of sites as energy resources, and potential hazards from hydrate dissociation or intense methane degassing from ocean warming. Stable i...

Trauma is a leading cause of death and morbidity worldwide. Here, we present the analysis of a longitudinal multi-omic dataset comprising clinical, cytokine, endotheliopathy biomarker, lipidome, metabolome, and proteome data from severely injured humans. A “systemic storm” pattern with release of 1,061 markers, together with a pattern suggestive of...

Background: Traumatic hemorrhage is the leading cause of preventable death, and its effects are often evident within the first 24 hours of hospital admission. We investigated the relationship between prehospital lactate measurement and administration of hospital blood products and life-saving interventions (LSIs) within 24 hours of hospital admissi...

Background Trauma elicits a complex inflammatory response that, among multiple presenting factors, is greatly impacted by the magnitude of injury severity. Herein, we compared the changes in circulating levels of mediators with known proinflammatory roles to those with known protective/reparative actions as a function of injury severity in injured...

Background Prehospital plasma administration during air medical transport reduces the endotheliopathy of trauma, circulating pro-inflammatory cytokines, and 30-day mortality among traumatically injured patients at risk of hemorrhagic shock. No clinical data currently exists evaluating the age of thawed plasma and its association with clinical outco...

Trauma is a leading cause of death and morbidity worldwide. The lack of a comprehensive characterization of the human response to severe injury limits progress in this field. Here, we analyzed a longitudinal, multi-omic dataset comprised of clinical, cytokine, endotheliopathy biomarker, lipidome, metabolome and proteome data from severely injured h...

Alterations in lipid metabolism have the potential to be markers as well as drivers of the pathobiology of acute critical illness. Here, we took advantage of the temporal precision offered by trauma as a common cause of critical illness to identify the dynamic patterns in the circulating lipidome in critically ill humans. The major findings include...

Methane is abundant in marine subsurface sediments, sourced from microbial or thermocatalytic production. The relative composition of its isotopologues (12 CH 4 , 13 CH 4 , 12 CH 3 D and 13 CH 3 D) is used to infer its sources and sinks. The anaerobic oxidation of methane (AOM) is an important methane sink reaction carried out by consortia of anaer...

Methane is abundant in marine subsurface sediments, sourced from microbial or thermocatalytic products. The relative composition of its isotopologues (12CH4, 13CH4, 12CH3D and 13CH3D) is used to infer its sources and sinks. The anaerobic oxidation of methane (AOM) is an important methane sink reaction carried out by consortia of anaerobic methanotr...

Background: Prehospital plasma improves survival for severely injured trauma patients transported by air ambulance. We sought to characterize the unexpected survivors, patients who survived despite having high predicted mortality following traumatic injury. Methods: The Prehospital Air Medical Plasma (PAMPer) trial randomized severely injured pa...

We examined the geochemistry and bacterial and archaeal community structure in the acidic (pH < 2.4) pit lake at Peña de Hierro, near the headwaters of the Río Tinto. The lake has strong vertical gradients in light, O2, pH, conductivity, and dissolved ions. Bacterial and archaeal communities between 0 and 32 m displayed low species richness and eve...

Background: Establishing the divergence times of groups of organisms is a major goal of evolutionary biology. This is especially challenging for microbial lineages due to the near-absence of preserved physical evidence (diagnostic body fossils or geochemical biomarkers). Horizontal gene transfer (HGT) can serve as a temporal scaffold between micro...

Life is ubiquitous in the environment and an important mediator of Earth's carbon cycle, but quantifying the contribution of microbial biomass and its metabolic fluxes is difficult, especially in spatially and temporally-remote environments. Microbes leave behind an often scarce, unidentifiable, or nonspecific record on geologic timescales. This th...

The objective of this crossover experiment was to investigate the effect of a methane inhibitor, 3-nitrooxypropanol (3NOP), on enteric methane emission, methane isotopic composition, and rumen fermentation and microbial profile in lactating dairy cows. The experiment involved 6 ruminally cannulated late-lactation Holstein cows assigned to 2 treatme...

Methane is an important energy resource and significant long-lived greenhouse gas. Carbon and hydrogen isotope ratios have been used to better constrain the sources of methane, but interpretations based on these two parameters alone can often be inconclusive. The precise measurement of a doubly-substituted methane isotopologue, 13CH3D, is expected...

Methane is an important constituent of the global carbon cycle. Nonetheless, the fluxes of methane are not well constrained, and it is often difficult to determine whether this gas is generated by microbial or geochemical (thermogenic or abiogenic) processes. The temperature of formation for methane, or temperature at which methane is equilibrated,...