Daniela Ramos Truzzi

Daniela Ramos Truzzi
University of São Paulo | USP · Department of Biochemistry (IQ)

B.S., M.S., Ph.D.

About

26
Publications
2,911
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
322
Citations
Additional affiliations
July 2018 - present
University of São Paulo
Position
  • Professor (Assistant)
February 2008 - February 2014
University of São Paulo
Position
  • PhD Student

Publications

Publications (26)
Article
High carbon dioxide tensions (hypercapnia) are toxic to mammals by both pH-dependent and pH-independent mechanisms that remain partially understood. Relevantly, carbon dioxide reacts with biologically ubiquitous oxygen metabolites such as peroxynitrite and hydrogen peroxide to produce carbonate radical and peroxymonocarbonate, respectively. These m...
Article
Full-text available
Protein S-nitrosation is an important consequence of NO●·metabolism with implications in physiology and pathology. The mechanisms responsible for S-nitrosation in vivo remain debatable and kinetic data on protein S-nitrosation by different agents are limited. 2-Cys peroxiredoxins, in particular Prx1 and Prx2, were detected as being S-nitrosated in...
Article
Dinitrosyl iron complexes (DNICs) are ubiquitous in mammalian cells and tissues producing nitric oxide (NO) and have been argued to play key physiological and pathological roles. Nonetheless, the mechanism and dynamics of DNIC formation in aqueous media remain only partially understood. Here, we report a stopped-flow kinetics and density functional...
Article
Full-text available
2-Cys peroxiredoxins (Prxs) rapidly reduce H2O2, thereby acting as antioxidants and also sensors and transmitters of H2O2 signals in cells. Interestingly, eukaryotic 2-Cys Prxs lose their peroxidase activity at high H2O2 levels. Under these conditions, H2O2 oxidizes the sulfenic acid derivative of the Prx peroxidatic Cys (CPSOH) to the sulfinate (C...
Article
Thiyl radicals are detected by EPR as co-products of dinitrosyl iron complex (DNIC) formation. In demonstrating that DNIC formation generates RS• in a NO rich environment, these results provide a...
Article
Full-text available
The scientific publication landscape is changing quickly, with an enormous increase in options and models. Articles can be published in a complex variety of journals that differ in their presentation format (online-only or in-print), editorial organizations that maintain them (commercial and/or society-based), editorial handling (academic or profes...
Article
Peroxiredoxins (Prx) are abundant thiol peroxidases that react rapidly with H2O2, constituting an important antioxidant defense and acting as sensors and transmitters of H2O2 signals in cells. Certain Prxs are readily inactivated by H2O2 at submillimolar concentrations, whereas others are less sensitive. This oxidative inactivation, or hyperoxidati...
Article
Full-text available
The ubiquitous cellular labile iron pool (LIP) is often associated with the production of the highly reactive hydroxyl radical, which forms through a redox reaction with hydrogen peroxide. Peroxynitrite is a biologically relevant peroxide produced by the recombination of nitric oxide and superoxide. It is a strong oxidant that may be involved in mu...
Article
Full-text available
Protein disulfide isomerase (PDI) is a ubiquitous dithiol-disulfide oxidoreductase that performs an array of cellular functions, such as cellular signaling and responses to cell damaging events. PDI can become dysfunctional by post-translational modifications, including those promoted by biological oxidants, and its dysfunction has been associated...
Article
Pseudomonas aeruginosa is a pathogen that needs to cope with the oxidative insult imposed by the mammalian host. Organic Hydroperoxide Resistance protein (Ohr) is a Cys-based peroxidase that together with its transcriptional regulator Organic Hydroperoxide Resistance Regulator (OhrR) comprises a system that is central to the P. aeruginosa response...
Article
Full-text available
Urate hydroperoxide is a product of the oxidation of uric acid by inflammatory heme-peroxidases. The formation of urate hydroperoxide might be a key event in vascular inflammation, where there is plenty amount of uric acid and inflammatory peroxidases. Urate hydroperoxide oxidizes glutathione and sulfur-containing amino acids and is expected to rea...
Article
Here, we report the synthesis, characterization, crystal data and DFT calculation of a new water-soluble ruthenium(II) carbonyl complex. The trans-[Ru(CO)(NH3)4P(O)(OH)2]Cl is a robust complex in aqueous media. The pKa of 3.3 for the phosphorous acid ligand in this complex was measured by infrared spectroscopy. The trans-[Ru(CO)(NH3)4P(O)(OH)2]⁺ ex...
Article
Protein S-nitrosation is a reversible post-translational modification that has been associated with a variety of physiological and pathophysiological processes. The mechanisms responsible for S - nitrosation in vivo , however, remain debatable. Dinitrosyl iron complexes (DNIC), which are consistently detected in cell and tissues overproducing nitri...
Article
This work evaluated the analgesic and anti-inflammatory activity of the ruthenium(II) complexes trans-[Ru(NO+)(NH3)4(L)](BF4)3 and [Ru(NH3)5(L)](BF4)3 containing the nonsteroidal anti-inflammatory drugs nicotinic acid (Hnic) and its isomer isonicotinic acid (ina) as ligands (L). The antinociceptive potential of these complexes and the free ligands...
Article
A estabilidade do ligante dietil fosfito foi analisada por 1H RMN em solução pH 1,0 e 3,0 a 25°C na presença e na ausência do íon [RuII(H2O)(NH3)5]2+. A hidrólise do dietil fosfito livre não foi observada em pH 3,0, enquanto na presença do íon [RuII(H2O)(NH3)5]2+, neste mesmo pH, esta reação foi observada (kobs=1,0.10-4 s-1). Em solução pH 1,0 a hi...
Article
Full-text available
Ruthenium compounds of the type trans-[Ru(NO)(NH 3) 4 (L)]X 3 , L ¼ N-heterocyclic ligands, P(OEt) 3 , SO 3 2À , X ¼ BF 4 À or PF 6 À , or [Ru(NO)Hedta], were tested for antitumour activity in vitro against murine melanoma and human tumour cells. The ruthenium complexes induced DNA fragmentation and morphological alterations suggestive of necrotic...
Article
The trans-[Ru(NO)(NH(3))(4)(P(OH)(3))]Cl(3) complex was synthesized by reacting [Ru(H(2)O)(NH(3))(5)](2+) with H(3)PO(3) and characterized by spectroscopic ((31)P-NMR, δ = 68 ppm) and spectrophotometric techniques (λ = 525 nm, ε = 20 L mol(-1) cm(-1); λ = 319 nm, ε = 773 L mol(-1) cm(-1); λ = 241 nm, ε = 1385 L mol(-1) cm(-1); ν(NO(+)) = 1879 cm(-1...
Article
Nitric oxide plays an important role in various biological processes, such as neurotransmission, blood pressure control, immunological responses, and antioxidant action. The control of its local concentration, which is crucial for obtaining the desired effect, can be achieved with exogenous NO-carriers. Coordination compounds, in particular rutheni...

Network

Cited By