Daniela Pinto

Daniela Pinto
Giuliani · R&D-Innovation Giuliani

PhD
Scientific Researcher and Chief Researcher Officer of HMAP (Human Microbiome Advanced Project)

About

71
Publications
18,635
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
882
Citations
Introduction
Daniela Pinto currently works at the R&D, Giuliani. Daniela does research in Food Science, Agronomy and Biotechnology. Their current project is 'Cutaneous disorder'.
Additional affiliations
March 2017 - present
Giuliani
Position
  • Rsearcher-Specialist in molecular biology
July 2013 - March 2017
Giuliani
Position
  • Researcher
January 2010 - June 2014
Università degli Studi di Bari Aldo Moro
Position
  • PostDoc Position

Publications

Publications (71)
Article
Full-text available
Introduction: Several options are available to treat acne lesions, including topical benzoyl peroxide, topical retinoids, topical antibiotics, oral antibiotics, hormonal therapy, isotretinoin, and procedural therapies, such as light and laser therapies, although these cause side effects. This study aimed to establish the efficacy and tolerability...
Article
Full-text available
Lichen Planopilaris (LPP) is a lymphatic disease affecting the scalp that is characterized by a chronic and destructive inflammation process, named as ‘cicatricial alopecia’ in which the hair follicles are targeted and may involve predominantly lymphocytes or neutrophils. Scalp and biopsy layers have never been used to investigate microbial communi...
Article
Background A gluten-free diet (GFD) is the main therapy for non-coeliac gluten sensitivity (NCGS). However, the availability of novel enzymes with the ability to digest gluten could represent a therapeutic opportunity for NCGS patients to avoid a GFD. Aims To evaluate the controlled reintroduction of gluten with or without the endopeptidase P1016...
Article
Full-text available
Celiac disease (CD) is an inflammatory autoimmune disorder triggered by the ingestion of gluten from wheat and other cereals. Nowadays, its positive diagnosis is based on invasive approaches such as the histological examination of intestinal biopsies and positive serology screening of antibodies. After proven diagnosis, the only admissible treatmen...
Article
Full-text available
The continuous research advances in the microbiome field is changing clinicians’ points of view about the involvement of the microbiome in human health and disease, including autoimmune diseases such as alopecia areata (AA). Both gut and cutaneous dysbiosis have been considered to play roles in alopecia areata. A new approach is currently possible...
Poster
The aim of this study is the development of a multi strain probiotic gel to promote lactobacilli-dominated vaginal microbiota in pregnant women and to establish a proper eubiosis on the new-born. Nomadic lactobacilli, mainly isolated from food sources, were screened for functional characteristics and the capability to inhibit Streptococcus agalacti...
Article
Full-text available
Introduction: Treatments other than topical and systemic antibiotics are needed to restore the dysbiosis correlated with acne onset and evolution. In this view, probiotics and botanical extracts could represent a valid adjunctive therapeutic approach. The purpose of this study was to test the efficacy of a dietary supplement containing probiotics...
Article
Full-text available
Background: Alopecia Areata (AA) is the first reported hair scalp disorder in children, with an onset mean age of 7.5 years. Potent topical corticosteroids are considered the first-line therapy with systemic glucocorticoids recommended only as a temporary measure in patchy AA in the active phase. Also, the efficacy of Janus kinase (JAK) inhibitors...
Poster
Full-text available
These studies showed that Celegyn® medical device could support the treatment of symptoms associated with VVA. It can decrease the inflammatory state of the in vitro vaginal epithelium, protecting it, and promoting repair mechanisms and wound healing. In addition, the clinical experience showed an improvement of the initial condition of VVA patient...
Poster
Full-text available
The expression of IL-23, by means of qRT-PCR, resulted higher in LPP samples than healthy subjects (Table 1). By using a linear regression model, we described those peculiar taxa that significantly differentiated LPP and healthy sample subgroups. At the phylum level three taxa were found as significantly different if considering the swab (Fig. 1)....
Article
Full-text available
Actinic keratosis is a form of dysplastic epidermal lesion resulting from chronic and excessive UV exposure with a certain risk of becoming cancerous. Current guidelines advocated the use of sunscreens to prevent photodamage. An efficient photoprotection must involve both primary protective factors such as UV filters and secondary factors (eg, anti...
Article
Full-text available
IntroductionActinic keratosis (AK) consists of skin lesions with a milder degree of keratinocytic atypia. It can be also referred to as “field of cancerization,” which can potentially evolve to cutaneous squamous cell carcinoma (SCC). Several therapeutic options are currently available, but not all are indicated on hyperkeratotic lesions. This stud...
Article
Full-text available
Fabio Rinaldi,1,2 Daniela Pinto,1,2 Anna Trink,2 Giammaria Giuliani,1 Adele Sparavigna3 1Giuliani SpA, Research and Development Department, Milan, Italy; 2IHRF, International Hair Research Foundation, Milan, Italy; 3Derming, Clinical Research, and Bioengineering Institute, Monza, ItalyCorrespondence: Fabio RinaldiGiuliani SpA, Research and Developm...
Article
Full-text available
Human skin is populated by various microorganisms, the so-called microbiota, such as bacteria, viruses, yeasts, fungi, and archaea. The skin microbiota is in constant contact with the surrounding environment which can alter its eubiotic state. Recently it has been also observed that the application of cosmetic products can alter the balance of the...
Article
Full-text available
IntroductionThe inflammation storm involved in coronavirus disease 2019 (COVID-19) infection and worsening and the psychological stress derived from current quarantine conditions can affect the course of many skin and scalp conditions. This study examined the possible effects of COVID‐19 on alopecia areata (AA) relapse in patients suffering from th...
Article
Full-text available
We compared raw bee-collected pollen (Raw-BCP), spontaneously fermented BCP (Unstarted-BCP), and BCP fermented with selected microbial starters (Started-BCP) to deepen whether fermentation may favorably affect the nutrients bioaccessibility and functional features of BCP. Under in vitro gastrointestinal batches, the highest serum-availability of ph...
Conference Paper
Several olfactory receptors (ORs) are expressed in human skin, which have been shown to regulate skin pigmentation, barrier, and regeneration, as well as hair growth. In particular, we reported that the activation of OR2AT4 up‐regulates the expression of the antimicrobial peptide (AMP) dermcidin (DCD) at gene and protein level in human scalp hair f...
Article
Full-text available
Although the hemp seed boasts high nutritional and functional potential, its use in food preparations is still underestimated due to scarce technological properties and the presence of several anti-nutritional factors. Here, an optimization of a biotechnological protocol aimed at improving the antioxidant properties and the protein digestibility of...
Article
Full-text available
Although the hemp seed boasts high nutritional and functional potential, its use in food preparations is still underestimated due to scarce technological properties and the presence of several anti-nutritional factors. Here, an optimization of a biotechnological protocol aimed at improving the antioxidant properties and the protein digestibility of...
Article
Full-text available
Nowadays, the involvement of the microbiome in human health and many human diseases, including that strictly related to the scalphas been brought to the light. Indeed, more recently, authors highlighted the presence of a significant microbial shift both in nonscarring (Androgenetic alopecia and Alopecia areata) and scarring Alopecias. The advent of...
Article
Wnt signalling is one of the most fundamental molecular pathways for modulating the human hair cycle and is essential for maintaining hair growth1 . We recently identified that the Wnt antagonist, secreted frizzled related protein 1 (SFRP1), prematurely induces catagen in microdissected human scalp hair follicles (HFs) ex vivo1 . SFRP1 is inhibited...
Article
Full-text available
Brewers’ spent grain (BSG) is the major by-product of the brewing industry which remain largely unutilized despite its nutritional quality. In this study, the effects of fermentation on BSG antioxidant potential were analyzed. A biotechnological protocol including the use of xylanase followed by fermentation with Lactiplantibacillus plantarum (Lact...
Article
Full-text available
The skin microbiome is in a very close mutualistic relationship with skin cells, influencing their physiology and immunology and participating in many dermatological conditions. Today, there is much interest in cosmetic ingredients that may promote a healthy microbiome, especially postbiotics, mainly derived from fermented products. In the present...
Article
Full-text available
Involvement of the microbiome in many different scalp conditions has been investigated over the years. Studies on the role of the scalp microbiome in specific diseases, such as those involving hair growth alterations like non-cicatricial [androgenetic alopecia (AGA), alopecia areata (AA)] and cicatricial alopecia lichen planopilaris, are of major i...
Article
IntroductionAlopecia areata (AA), also known as ‘area Celsi’, is the second most common form of hair loss affecting the scalp. Newly proposed treatments for AA include low-level light therapy, biologics such as Janus kinase inhibitors and autologous platelet-rich plasma (PRP), which is a well-known “elixir” for hair growth. Bioactive peptides devel...
Article
Full-text available
Alopecia areata (AA) is a potentially reversible auto-immune non-scarring baldness on the scalp, which can be extended to the entire body. There are many scientific evidence as regards the impact of diet on scalp diseases related to hair growth. Diet is also able to strongly influence gut microbiome. On the contrary, few evidence reports as regards...
Article
Full-text available
Introduction: The impact of diet on hair growth disorder is well established as the influence of diet on the gut microbiome. Poor information is still available as regards the link between microbiome, especially scalp microbiome and hair diseases. Aim: In the present work, we reported data on patients affected by Alopecia areata with the aim to stu...
Article
Full-text available
An increasing consumer demand for pomegranate has been globally observed, mainly thanks to the scientific evidence related to its functional and health-promoting features. Pomegranate fruits from twenty accessions identified in Southeastern Italy were characterized according to morphological and chemical features. Juices extracted from pomegranate...
Article
Full-text available
The role of microbial dysbiosis in scalp disease has been recently hypothesized. However, little information is available with regards to the association between microbial population on the scalp and hair diseases related to hair growth. Here we investigated bacterial communities in healthy and Alopecia areata (AA) subjects. The analysis of bacteri...
Article
Full-text available
Introduction: Androgenetic alopecia (AGA) is the most common hair loss disorder. Recently, platelet-rich plasma (PRP) injections have emerged as alternative cell-based therapies for the treatment of AGA. Its efficacy is strictly linked to the release of growth factors (GFs) via alpha-granules degranulation. Among their well-known activity, more rec...
Article
Full-text available
Background Alopecia areata (AA) is a non-scarring auto-immune hair disorder. Recent researches explained the role of growth factors (GFs) in hair follicle cycling. The main reservoir of GFs are alpha-granules of platelets and novel procedures have been implemented aimed at collecting platelet-rich plasma (PRP). PRP has been safely implemented in ma...
Article
Full-text available
Actinic keratosis (AK) is a chronic and recurrent disease and in this double-blind, half face, placebo controlled study we attempt to examine the efficacy of a topical treatment containing 2,4,6-octatrienoic acid and anthraquinone knipholone for the prevention of new AK lesion formations. New lesion onset was detected with methyl-5-aminolevulinic a...
Article
Introduction: Actinic keratosis (AKs) are epidermal lesions that commonly occur in skin exposed to chronic cumulative UV irradiation. Untreated AK lesions can advance to squamous cell carcinoma. Current treatments of AK have many shortcomings; for instance, not all treatments can be used for the hyperkeratotic form of AK. The aim of this study was...
Article
Full-text available
Interleukin-17 (IL-17) has been implicated in the pathogenesis of a large number of inflammatory and autoimmune conditions, including skin disorders such as psoriasis. Recently, much data have accumulated on the possible role of IL-17 in the pathogenesis of alopecia areata (AA). In this review, the available information on the connection between AA...
Article
Background: Considering the importance of hair in our modern society and the impact of hair loss, the efforts of researchers are addressed to better understand the mechanisms behind the hair cycle regulation and dysregulation. Because hair loss is multifactorial, differenced and new approaches are required. In particular we addressed our attention...
Article
Full-text available
Nowadays, the study of human microbiome represents a novel diagnostic and therapeutic approach to treat many human conditions, also including that strictly related to skin and scalp. The findings we included in the present work represent just an overview of a larger pioneer study on the involvement of changing of the microbiome in scalp diseases,...
Article
Full-text available
Introduction: Treatment with finasteride 1 mg/day represents the therapy of choice for androgenetic alopecia (AGA). We investigated how Italian dermatologists approach use of finasteride for treatment of AGA and common side effects reported by patients. Methods: A tablet-based survey was conducted from February 2017 to January 2018 in Italy to i...
Article
Full-text available
Regenerative medicine is a modern approach of dermatological treatment, using Epidermal Cells of the interfollicular epidermis (ESCs) for their effect in skin regeneration in chronic ulcers and burns, melanoma, vitiligo, junctional epidermolysis bullosa. Intraepidermal injections of autologous epidermal cell suspension can be a new and very promisi...
Article
Full-text available
Lichen Planopilaris is known as the form of Lichen Planus typical of the scalp. It is classifi ed as a lymphatic disease and is characterized by chronic infl ammation which leads to cicatricial alopecia. Its causes are not yet well characterized but its etiology seems to strongly correlates with infection, sensitization and pollution. A clear and o...
Article
Full-text available
Background: Spermidine has been shown both in vitro and in mice models to have an anagen-prolonging effect on hair follicles (HFs). Objectives: To evaluate the effects of a spermidine-based nutritional supplement on the anagen phase of HFs in healthy human subjects in a randomized, double-blind, placebo-controlled trial. Methods: One hundred h...
Article
Full-text available
In the present study the esterification of the hydroxyl groups of resveratrol, caffeic acid, ferulic acid and ß-sitosterol with an antioxidant polyconjugated fatty acid, 2E,4E,6E-octatrienoic acid, was achieved. As the selective esterification of hydroxyl groups of natural compounds can affect their biological activity, a selective esterification o...
Article
Full-text available
Background: Lactoferrin (LF)is an innate-defence non-heme iron binding glycoprotein of 80 kDa able to support the immune system and influence immune cell activity by antioxidant, antibacterial and antiviral properties. This work focusses on the study of the in vitro anti-inflammatory activity of Bovine LF(bLF)on Lipopolysaccharide (LPS)-induced cyt...
Article
This study aimed at using grape marc for the growth of lactic acid bacteria and bifidobacteria with the perspective of producing a functional ingredient having antioxidant activity. Lactobacillus plantarum 12A and PU1, Lactobacillus paracasei 14A, and Bifidobacterium breve 15A showed the ability to grow on grape marc (GM) based media. The highest b...
Article
Full-text available
Background: There is an increasing interest toward the use of legumes in food industry, mainly due to the quality of their protein fraction. Many legumes are cultivated and consumed around the world, but few data is available regarding the chemical or technological characteristics, and especially on their suitability to be fermented. Nevertheless,...
Article
Spermidine (Spd), the prototypic polyamine, has been shown to be essential for hair follicle (HF) growth. However, Spd can be readily converted into other polyamines, and is physiologically unstable. Therefore, to assess its individual functions on HFs, we used the metabolically stable Spd analog N(1)-methylspermidine (N(1)-MeSpd). N(1)-MeSpd was c...
Article
Full-text available
Background Myrtle (Myrtus communis L.) is a medicinal and aromatic plant belonging to Myrtaceae family, which is largely diffused in the Mediterranean areas and mainly cultivated in Tunisia and Italy. To the best of our knowledge, no studies have already considered the use of the lactic acid fermentation to enhance the functional features of M. com...
Article
Full-text available
Background Extracts and products (roots and/or aerial parts) from Echinacea ssp. represent a profitable market sector for herbal medicines thanks to different functional features. Alkamides and polyacetylenes, phenols like caffeic acid and its derivatives, polysaccharides and glycoproteins are the main bioactive compounds of Echinacea spp. This stu...
Article
Plantaricin A (PlnA) is a peptide with antimicrobial and pheromone activities. PlnA was synthesized chemically and used as a pure peptide or synthesized biologically using Lactobacillus plantarum DC400 co-cultured with Lactobacillus sanfranciscensis DPPMA174. Cell-free supernatant (CFS) was used as a crude PlnA preparation. As estimated using the 3...