Daniela Palheiro Mendes de Almeida

Oswaldo Cruz Foundation | FIOCRUZ · Instituto de pesquisa Clínica Evandro Chagas (IPEC)

The diagnostic variables to distinguish myeloproliferative disorders (MPD) and acute myeloid leukemia (AML) is still a challenge in developing countries. We aimed to explore the prevalence of RAS pathways mutations with a focus on PTPN11 mutation in MPD and AML affecting pediatric patients. RAS and PTPN11 mutations were found in 10.4% and 3.3% of A...

Background The emergence of new variants of SARS-CoV-2 has led to the administration of different booster vaccines to mitigate COVID-19. Vaccines with adenoviral vectors have been rarely associated with vaccine-induced immune thrombotic thrombocytopenia (VITT). Objectives This study aimed to describe 15 cases of VITT after the third and fourth dos...

Methods: Inflammatory gene expression and LPS responses was determined in monocytes from WT and thrombocytopenic mice. Mouse sepsis was induced by LPS or cecal slurry model (Fig 1). In vitro, mouse monocytes were co-cultured with platelets overnight, platelets washed away and monocytes were LPS stimulated. RNA-Seq was performed on platelet incubate...

Background: Females with sickle cell disease (SCD) experience more frequent and severe vaso-occlusive crises (VOC) than males. Additionally, many females with SCD report perimenstrual timing of VOC, suggesting cyclic variation in their pain risk. C-reactive protein (CRP) is a robust inflammatory marker that is elevated at baseline in SCD patients a...

Background Familial aggregation in childhood leukemia is associated with epidemiological and genomic factors. Albeit epidemiological studies on the familial history of hematological malignancies (FHHMs) are scarce, genome-wide studies have identified inherited gene variants associated with leukemia risk. We revisited a dataset of acute lymphoblasti...

The emergence of the rare syndrome called vaccine-induced immune thrombocytopenia and thrombosis (VITT) after adenoviral vector vaccines, including ChAdOx1 nCov-19, raises concern about one's predisposing risk factors. Here we report the case of a 56-year-old white man who developed VITT leading to death within 9 days of symptom onset. He presented...

Parvovirus B19 (B19V) infection varies clinically depending on the host’s immune status. Due to red blood cell precursors tropism, B19V can cause chronic anemia and transient aplastic crisis in patients with immunosuppression or chronic hemolysis. We report three rare cases of Brazilian adults living with human immunodeficiency virus (HIV) with B19...

Introdução Nos dias atuais, a transfusão de sangue é uma terapêutica frequentemente utilizada em pacientes internados e também, amplamente empregada em procedimentos cirúrgicos. Embora tenha benefícios inegáveis, não é isenta de riscos e efeitos colaterais de gravidades variáveis. No período intraoperatório é fundamental avaliar a real necessidade...

Introduction Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked, hereditary condition caused by mutations in the G6PD gene, that results in protein variants with different enzyme activity levels. G6PD provides reducing power to NADPH (reduced form of nicotinamide adenine dinucleotide phosphate). NADPH effect is critical in red blood...

Introduction Parvovirus B19 (B19V) infection could cause clinical manifestations depending on the host‘s immunologic and hematologic status. In immunocompetents individuals, clinical manifestations range from asymptomatic, or flu-like symptoms in childhood including a viral exanthem (fifth disease) to a fatal hydrops fetalis in pregnant women. B19V...

Objetivos Investigar os aloanticorpos irregulares mais prevalentes em pacientes atendidos na Agência Transfusional do Centro Hospitalar do Instituto Nacional de Infectologia Evandro Chagas/INI-Fiocruz durante o período de pandemia do COVID-19. Material e métodos Trata-se de um estudo observacional retrospectivo realizado pela coleta de informações...

Introdução O Business Intelligence (BI) combina análise, visualização de dados, ferramentas/infraestrutura de dados e práticas recomendadas para ajudar as organizações a tomarem decisões impulsionadas por dados. Na prática, um BI moderno em gestão de hemoterapia possui uma visão abrangente dos dados da organização e usa esses dados para gerar mudan...

The use of antiretroviral therapy has drastically improved the life quality and prognosis of people living with the human immunodeficiency virus (HIV). The risk of acute myeloid leukemia (AML) currently does not appear to be significantly increased compared to the general population. Acute promyelocytic leukemia (APL), infrequent in people with HIV...

Objective Describe a case series of vaccine-induced immune thrombotic thrombocytopenia (VITT) after COVID-19 vaccination in Brazil that included ChAdOx1 nCoV-19, Ad26.COV2.S and BNT162b2 vaccines, describing their clinical and laboratory characteristics. Methodology Descriptive case series study using Bio-Manguinhos/Fiocruz/AstraZeneca Brazil and...

Reports of side effects of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are increasing worldwide. Capillary leak syndrome and vaccine-induced immune thrombotic thrombocytopenia are very rare but life-threatening adverse events that should be identified early and treated. However, isolated thrombocytopenia can indica...

An increased number of adverse events following immunization (AEFI) reports are expected during mass vaccination campaigns. Considering this current situation, the need to implement additional strategies for an effective benefit-risk assessment of the Covid-19 vaccine arose. One of the actions initiated by the Clinical Advisory Unit was the establi...

Introduction Haploinsufficiency of the hematopoietic transcription factor GATA2 is associated with a broad spectrum of diseases, including infection susceptibility and neoplasms. We aimed to investigate GATA2 variants in patients with non-tuberculous mycobacterial (NTM) and/or fungal infections (FI) without known immunodeficiencies. Method We perf...

p>Acute leukemia (AL) is the most common type of childhood cancer and has two major subtypes: acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). Historically, studies in this field have pointed out driver somatic mutations with different mechanisms of pathogenesis. Family-based and large-scale high-throughput sequencing studies ha...

Childhood leukemias are heterogeneous diseases with widely differing incident rates worldwide. As circulating tumors, childhood acute leukemias are uniquely accessible, and their natural history has been described in greater detail than for solid tumors. For several decades, it has been apparent that most cases of childhood acute lymphoblastic leuk...

[This corrects the article DOI: 10.1371/journal.pone.0245458.].

Introduction Anemia is a common condition at tuberculosis diagnosis, and there is evidence that its prevalence is higher in patients with tuberculosis than in those infected with Mycobacterium tuberculosis and healthy controls. Information about anemia during tuberculosis diagnosis is still scarce in the Brazilian population. The aim of this study...

Background Non-tuberculous mycobacteria (NTM) and invasive fungal infections (IFI) may be considered opportunistic infections in immunocompromised patients with GATA2 haplodeficiency. Sporadic or familial GATA2 mutations are associated with infection susceptibility, autoimmunity, and myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML)....

Poster presented by Filipe V. Santos-Bueno

Background: GATA2 is a transcription factor that is a critical regulator of gene expression in hematopoietic cells. GATA2 deficiency presents with multi-lineage cytopenia, mycobacterial, fungal and viral infections. Patients with GATA2 mutation have a high risk of developing myelodysplastic syndrome or acute myeloid leukemia. Case presentation: We...

Background: GATA2 is a transcription factor that is a critical regulator of gene expression in hematopoietic cells. GATA2 deficiency presents with multi-lineage cytopenia, mycobacterial, fungal and viral infections. Patients with GATA2 mutation have a high risk of developing myelodysplastic syndrome or acute myeloid leukemia. Case presentation:...