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Introduction
Publications
Publications (13)
Changes in habitat characteristics are known to have profound effects on biotic communities and their functional traits. In the context of an urban-rural gradient, urbanisation drastically alters abiotic characteristics, e.g., by increasing environmental temperatures and through light pollution. These abiotic changes significantly impact the functi...
Temperature and competition are two of the main factors determining ant community assemblages. Temperature may allow species to forage more or less efficiently throughout the day (in accordance with the maximum activity temperature of each species). Competition can be observed and quantified from species replacements occurring during resource explo...
The queen of Lasius piliferus Seifert, 1922 is described. A total of 35 localities from bibliographical records are listed, and 40 new ones are added, including the first records from the provinces of A Coruña, Barcelona, Cantabria, Girona, Guadalajara, Lugo, Ourense, Palencia and Zamora. The distribution, habitats and phenology of the species are...
First iberian record for Temnothorax conatensis Galkowski & Lebas, 2016, and update of the distribution for Temnothorax grouvellei (Bondroit, 1918) (Hymenoptera: Formicidae).
Temnothorax ibericus Menozzi is an Iberian endemic species. The scarce published data are updated with several new localities, showing it has an orophilous distribution. New phenology and nesting data are also contributed. The previously unknown queen is described.
Resumen (Español): Se añade Bothriomyrmex communista, una especie parásita social temporal, al listado de las especies conocidas para España y la península ibérica. Se comenta la situación taxonómica y nomenclatural muy particular del nombre específico Bothriomyrmex rogeri Emery, 1924. Palabras clave Bothriomyrmex, parásita social temporal, Sierra...
El creciente interés por el medio ambiente y su conservación ha llevado al aumento de trabajos sobre biodiversidad. Desde el punto de vista mirmecológico, esta tendencia está proporcionando nuevos datos a nivel mundial, situándose la península ibérica como el territorio europeo con mayor biodiversidad de formícidos. El aumento de trabajos en la pen...
La Sierra de Javalambre, situada al sur de la provincia de Teruel, con sus 2020 m en el pico Javalambre, representa una de las cotas de mayor altitud del Sistema Ibérico. Este entorno forma un ecosistema de adversas condiciones para muchos organismos debido a sus bajas temperaturas. Encontramos heladas desde noviembre hasta mayo, y veranos suaves,...
The exotic ant species Cardiocondyla obscurior is added to the checklist of ants known for Spain. Workers, a wingless queen and a winged male, were captured within the university campus at Burjassot (Valencia).
Questions
Questions (2)
I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of cells by extracting DNA from the maximum amount of sample possible. QIAamp Mini kit recommend using a maximum of 200ul of liquid sample for the extraction, but I contacted the company and they told me I can increase it until 400ul if keep the proportions by doubling the volume of the kit reagents and pass all the liquid through the membrane in two centrifugation steps.
Taking into account DNAShield help in lysis cells and inactivate DNAses, could I skip some of the lysis buffers and proteinase K used in the first steps of the kit protocol by DNAshield and increase the volume of sample passed thought the silica membrane?
I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of cells by extracting DNA from the maximum amount of sample possible. QIAamp Mini kit recommend using a maximum of 200ul of liquid sample for the extraction, but I contacted the company and they told me I can increase it until 400ul if keep the proportions by doubling the volume of the kit reagents and pass all the liquid through the membrane in two centrifugation steps.
I aim to minimize both the amount of liquid and beads to be able to use the maximum amount of sample during DNA extraction and reduce the loss of DNA from my low concentrated samples. So, I would like to perform bead-beating prior DNA extraction in the minimum about of liquid and beads.
Do you know what is the minimum amount of liquid and beads (0.1 + 0.5mm) I can use for bead-beating in a 2ml vial?
