Daniel Marquina

• PhD
• Project Specialist at AllGenetics

Emerald ash borer beetles (Agrilus planipennis) in North America are a destructive invasive species that increase tree mortality continent-wide, resulting in major ecological and economic impacts. Trees that are infested experience mortality rates which can exceed 99%, disrupting ecological communities and threatening the $218 billion forestry indu...

Species interactions are challenging to quantify, particularly when they happen cryptically. Molecular methods have become a key tool to uncover these interactions when they leave behind a DNA trace from the interacting organism ( e.g ., pollen on a bee) or when the taxa are still present but morphologically challenging to identify ( e.g ., microbi...

We present the data from the Insect Biome Atlas project (IBA), characterizing the terrestrial arthropod faunas of Sweden and Madagascar. Over 12 months, weekly Malaise trap samples were collected at 203 locations within 100 sites in Sweden and at 50 locations within 33 sites in Madagascar; this was complemented by soil and litter samples from each...

Increasing food production while avoiding negative impacts on biodiversity constitutes one of the main challenges of our time. Traditional silvopastoral systems like Iberian oak savannas ("dehesas") set an example, where free-range livestock has been reared for centuries while preserving a high natural value. Nevertheless, factors decreasing produc...

Dalerum, Fredrik (2023). Data for Måsviken et al. 2023 Ecosphere 14:e4540.

Mountain topography gives rise to often dramatic climate-driven elevation gradients in primary productivity, which can generate substantial biodiversity variation. Therefore, mountain areas may be particularly useful for evaluating the ecological consequences of climate change. Arthropods are the most diverse animal phylum, which play important rol...

DNA metabarcoding can accelerate research on insect diversity, as it is cheap and fast compared to manual sorting and identification. Most metabarcoding protocols require homogenisation of the sample, preventing further work on the specimens. Mild digestion of the tissue by incubation in a lysis buffer has been proposed as an alternative, and, alth...

Traditionally, insects collected for scientific purposes have been dried and pinned, or preserved in 70% ethanol. Both methods preserve taxonomically informative exoskeletal structures well but are suboptimal for preserving DNA for molecular biology. Highly concentrated ethanol (95–100%), preferred as a DNA preservative, has generally been assumed...

As global biodiversity declines, there is an increasing need to create an educated and engaged society. Having people of all ages participate in measuring biodiversity where they live helps to create awareness. Recently, the use of environmental DNA (eDNA) for biodiversity surveys has gained momentum. Here, we explore whether sampling eDNA and sequ...

Background: As global biodiversity declines, there is an increasing need to create an educated and engaged society. Having people from all ages participate in measuring biodiversity where they live helps to create awareness. Recently, the use of environmental DNA (eDNA) for biodiversity surveys has gained momentum. Here, we test whether sampling eD...

1. Traditionally, insects collected for scientific purposes have been dried and pinned, or preserved in 70 % ethanol. Both methods preserve taxonomically informative exoskeletal structures well but are suboptimal for preserving DNA. Highly concentrated ethanol (95 - 100 %), preferred as a DNA preservative, has generally been assumed to make specime...

DNA metabarcoding allows the analysis of insect communities faster and more efficiently than ever before. However, metabarcoding can be conducted through several approaches, and the consistency of results across methods has rarely been studied. We compare the results obtained by DNA metabarcoding of the same communities using two different markers...

Background: Metabarcoding of the DNA extracted from preservative ethanol of insect bulk samples, instead of homogenized tissue, could bring a series of advantages such as reducing processing time and handling (reducing risk of cross-contamination). But, more importantly, it would allow researchers to conduct further work on intact insects (taxonomy...

Massively parallel DNA sequencing opens up opportunities for bridging multiple temporal and spatial dimensions in biodiversity research, thanks to its efficiency to recover millions of nucleotide polymorphisms. Here, we identify the current status, discuss the main challenges, and look into future perspectives on biodiversity genomics focusing on i...

Raw data retrieved from our search in Web of Science. Our Literature Review was based on an unbiased search in Web of Science. More details can be found in the main text.

Input data for generating Figure 3. Data was retrieved from Web of Science. The analyses were performed only on Original research. Other type of articles retrieved by our search in Web of Science can be found in the raw data file in Supporting Material.

DNA metabarcoding allows the analysis of insect communities faster and more efficiently than ever before. However, metabarcoding can be conducted through several alternative approaches, and the consistency of results across methods has rarely been studied. We compare the results obtained by DNA metabarcoding of the same communities using two differ...

Massively parallel DNA sequencing opens up opportunities for bridging multiple temporal and spatial dimensions in biodiversity research, thanks to its efficiency to recover millions of nucleotide polymorphisms. Here we identify the current status, discuss the main challenges, and look into future perspectives on biodiversity genomics focusing on in...

Massively parallel DNA sequencing opens up opportunities for bridging multiple temporal and spatial dimensions in biodiversity research, thanks to its efficiency to recover millions of nucleotide polymorphisms. Here we identify the current status, discuss the main challenges, and look into future perspectives on biodiversity genomics focusing on in...

Insect metabarcoding has been mainly based on PCR amplification of short fragments within the ‘barcoding region’ of the gene COI. However, because of the variability of this gene, it has been difficult to design good universal PCR primers. Most primers used today are associated with gaps in the taxonomic coverage or amplification biases that make t...

The desing of PCR primers is a key step in the process of metabarcoding, upon which the rest of the study has to be build. A flaw in the primer design will lead to a poor recovery of some taxonomic groups, sometimes even missing species, or unspecific amplification that might end in the same result. Thus, it is very important to use the right prime...

Insect metabarcoding has been mainly based on PCR amplification of short fragments within the 'barcoding region' of the gene COI. However, because of the variability of this gene, it has been difficult to design good universal PCR primers. Most primers used today are associated with gaps in the taxonomic coverage or amplification biases that make t...

The study of biodiversity within the spatiotemporal continuum of evolution, e.g., studying local communities, population dynamics, or phylogenetic diversity, has been important to properly identify and describe the current biodiversity crisis. However, it has become clear that a multi-scale approach – from the leaves of phylogenetic trees to its de...

The study of biodiversity within the spatiotemporal continuum of evolution, e.g., studying local communities, population dynamics, or phylogenetic diversity, has been important to properly identify and describe the current biodiversity crisis. However, it has become clear that a multi-scale approach-from the leaves of phylogenetic trees to its deep...

Background: Many universal primers for insect metabarcoding have been reported to introduce amplification bias due to mismatches with the templates. In a diverse sample, such as a soil or Malaise trap sample of insects, this may result in some species being less efficiently amplified or not detected at all. We explored the potential of solving this...

A phylogenetic analysis of Polycladida based on two partial mitochondrial genes (cox1 and 16S) is provided. The analysis includes 30 polyclad terminals that represent species from the two taxa which traditionally divide the groups Cotylea and Acotylea. Our phylogenetic analyses pro- duced a well-supported hypothesis that confirms the mono- phyly of...

In the present work eleven polyclad species of Lizard Island are studied. Seven of them are new records for this locality of the Australian coral reef and one is new to science, Lurymare clavocapitata n. sp. (Family Prosthiostomidae). The remaining recorded species belong to the genera Pseudoceros (P. bimarginatus, P. jebborum, P. stimpsoni, P. zeb...

The Iberian Peninsula is part of the South European Atlantic Shelf within the Lusitanian ecoregion. Given the characteristics of this region, a great invertebrate biodiversity is expected. Nevertheless, no bibliographic records of Polycladida are known for the Cantabrian Sea. Here, we report the presence of six polyclad species, including one new s...

Las comunidades de meiofauna de un puerto poco perturbado (puerto de Marina del Este) y una zona control (Playa Galera) en la costa subtropical de Almunecar (Espana) fueron comparadas para evaluar la utilidad de la meiofauna a niveles taxonomicos superiores como herramienta de monitoreo ambiental en puertos. En la zona control se muestrearon tres e...

Actualmente la familia Pleioplanidae se encuentra dividida en 3 géneros: Pleioplana Faubel, 1983, Melloplana Faubel, 1983 e Izmira Bulnes, 2010 (Faubel, 1983; Bulnes, 2010). En este trabajo se ha llevado a cabo la revisión de esta familia, empleando especímenes del océano Atlántico Norte provenientes de las costas españolas de Galicia y Asturias. E...

Los representantes de la Familia Pseudocerotidae Lang,1884 (Platyhelminthes, Polycladida) se pueden encontrar en casi todas los mares del globo, pero son especialmente abundantes en aguas tropicales. Los géneros de esta familia son fácilmente distinguibles entre sí por su morfología, mientras que dentro de cada género existe una gran homogeneidad r...

El primer estudio exhaustivo sobre policládidos se realizó dentro de la cuenca mediterránea y concretamente en el Golfo de Nápoles (Lang, 1884), desde entonces los estudios sistemáticos, de distribución o taxonómicos han sido puntuales, y muchas de las especies, e incluso géneros, solo son citados en la descripción original. A pesar que el orden Po...

Sistem Sistemá ática filogen tica filogené ética del Orden Polycladida tica del Orden Polycladida (Platyhelminthes) enraizada en la taxonom (Platyhelminthes) enraizada en la taxonomí ía morfol a morfoló ógica gica y molecular. y molecular. Phylogenetic Systematics of the Order Polycladida (Platyhelminthes) rooted in morphological and molecular taxo...

Depth and sediment granulometry effects on subtidal meiofaunal assemblages of the subtropical coast of Granada (Alboran Sea) Efectos de la profundidad y granulometría en comunidades submareales de meiofauna de la costa subtropical de Granada (Mar de Alborán) ABSTRACT Hypotheses about relationships of meiofaunal assemblages to depth and sediment gra...

A study of polyclad fauna of the Atlantic coast of the Iberian Peninsula was carried out from 2010 to 2013. The paper reports nine new records belonging to three Cotylean families: the family Euryleptidae Lang, 1884, Pseudocerotidae Lang, 1884 and the family Prosthiostomidae Lang, 1884, and describes one new species, Euryleptodes galikias sp. n.

Along the Mediterranean coast of the Iberian Peninsula, great species diversity is thought to exist, but our knowledge of Iberian polyclads is, in fact, very limited. This study contributes to the Polycladida (Platyhelminthes) of the Iberian Peninsula, in particular those of the Mediterranean coast. Nine species, mainly new species or new records,...

Podemos suponer que en las costas de la Península Ibérica existe una gran diversidad de especies pertenecientes al Orden Polycladida, pero de hecho, no sólo se desconoce esta diversidad, sino que se carecen de registros fidedignos. En este estudio se describen por primera vez representantes del Orden Polycladida en el entorno ibero-atlántico. Duran...

Primeras citas y nuevas especies del Orden Polycladida (Platyhelmintha) para las costas atlánticas de la Península ibérica. FINANCIAMENTO INTRODUCCIÓN Podemos suponer que en las costas de la Península Ibérica existe una gran diversidad de especies pertenecientes al Orden Polycladida, pero de hecho, no sólo se desconoce esta diversidad, sino que se...

The Polycladida is a group of marine flatworms with more than 900 species and a world wide distribution. This group was firstly described in 1881, and since then, there have been several conflicting classifications, some of them still coexist nowadays. These previous and current taxonomic disagreements have made difficult the understanding of evolu...

Although the order Polycladida is known for the Mediterranean Sea for nearly 200 years, most of the studies have been focused in a few locations in the Adriatic Sea and the Gulf of Naples (Lang,1884). On the east, south and west coasts, works of Bulnes (2009), Gammoudi et al. (2009) and Novell (2003), respectively, have been carried out, showing hi...