Daniel Krüzselyi

Plant Protection Institute, Center for Agricultural Research, Budapest

Thirteen root extract samples of four goldenrod ( Solidago ) species present in Europe were investigated by hyphenated high-performance thin-layer chromatography (HPTLC). Only S. virgaurea is native, whereas S. gigantea , S. canadensis, and S. graminifolia have been introduced from North America. The bioactive zones in the Aliivibrio fischeri bioau...

Background: Batrachochytrium dendrobatidis (Bd) is the causative agent of chytridiomycosis, one of the major causes of worldwide amphibian biodiversity loss. Many amphibians exhibit skin-based chemical defences, which may play an important role against invading pathogens, but whether the synthesis of these chemical compounds is enhanced or suppres...

Eight bioactive clerodane diterpenes from the root extract of Solidago gigantea Ait. (giant goldenrod) were quantified by high-performance thin-layer chromatography (HPTLC) and two newly developed hyphenated methods. One uses vanillin sulphuric acid derivatization and densitometry, and the other an inhibition assay of acetylcholinesterase (AChE) an...

Bioactive clerodane diterpenes of giant goldenrod (Solidago gigantea Ait.) root extract Ágnes M. Móricz, Dániel Krüzselyi, Péter G. Ott, Zsófia Garádi, Szabolcs Béni, Gertrud E. Morlock, József Bakonyi Abstract Giant goldenrod (Solidago gigantea Ait.) root extract was screened for bioactive compounds by high-performance thin-layer chromatograph...

Many organisms synthesize secondary metabolites against natural enemies. However, to which environmental factors the production of these metabolites is adjusted to is poorly investigated in animals, especially so in vertebrates. Bufadienolides are steroidal compounds that are present in a wide range of plants and animals and, if present in large qu...

Antioxidant parameters of extracts from whole fruiting bodies and different morphological tissues (peel, inner cap, gills and stipes) of eight popular edible mushrooms were determined and compared. The examined species of both cultivated and wild mushrooms were analysed for total phenolic content (TPC) and free radical scavenging activity (RSA) by...

A high-performance thin-layer chromatography (HPTLC) method was developed for rapid and easy-to-perform discrimination between five goldenrod species present in Europe: the native Solidago virgaurea and the four invasive aliens, S. canadensis, S. gigantea, S. rugosa and S. graminifolia. The chemotaxonomic distinction was based on the chemical profi...

Effect-directed isolation of free radical scavengers from the methanol extract of the freeze-dried fruiting bodies of the cultivated basidiomycetous mushroom, black poplar (Cyclocybe cylindracea), yielded a β-carboline alkaloid. Its structure was determined based on ESI-TOF-MS/MS, NMR and circular dichroism spectra by comparison with published data...

Paulownia tomentosa (Thunb.) Steud. is a medicinal plant with a very long established and considerable position in traditional Chinese medicine (TCM) and in those of the other Far East Asian countries (mainly in Vietnam, Laos, Japan, and Korea). In Europe, Americas and Australia, P. tomentosa is known as princess tree, empress tree, or foxglove tre...

Many organisms use inducible defenses as protection against predators. In animals, inducible defenses may manifest as changes in behavior, morphology, physiology, or life history, and prey species can adjust their defensive responses based on the dangerousness of predators. Analogously, prey may also change the composition and quantity of defensive...

In this study, the antibacterial profiling of the ethanolic leaf extract of greater burdock (Arctium lappa L.) is demonstrated, applying thin-layer chromatography (TLC) coupled bioassays against the Gram-positive soil bacterium Bacillus subtilis and the Gram-negative pepper pathogen Pseudomonas syringae pv. maculicola. The main active component was...

Many animals display spectacular adjustments of their phenotype to changing environmental conditions in terms of altered behaviour, physiology and morphology, but whether similar plasticity in chemical defences is taxonomically widespread as well has remained unknown. Bufadienolides, toxins produced by some anuran amphibians, are known to be effect...

The antibacterial profiling of Onopordum acanthium L. leaf extract and subsequent targeted identification of active compounds is demonstrated. Thin-layer chromatography (TLC) and off-line overpressured layer chromatography (OPLC) coupled with direct bioautography were utilized for investigation of the extract against eight bacterial strains includi...

Chemical pollutants can exert various sublethal effects on wildlife, leading to complex fitness consequences. Many animals use defensive chemicals as protection from predators and diseases, yet the effects of chemical contaminants on this important fitness component are poorly known. Understanding such effects is especially relevant for amphibians,...

Background Chemical defences are widespread in animals, but how their production is adjusted to ecological conditions is poorly known. Optimal defence theory predicts that inducible defences are favoured over constitutive defences when toxin production is costly and the need for it varies across environments. However, if some environmental changes...

Overpressured layer chromatography (OPLC), ensuring pumpforced constant mobile phase flow and the possibility of overrun, offers the expanded exploitation of fne-particle adsorbent layers for a longer development distance. Using an infusion-transfusion OPLC method with a 26-cm long development, the separation of clove, rosemary, eucalyptus, tea tre...

A non-targeted, effect-directed screening (bioprofiling) and a subsequent highly targeted characterization of antibacterial compounds from plant matrices is demonstrated on the example of Solidago virgaurea root extracts. The procedure comprises high-performance thin-layer chromatography (HPTLC) coupled with six bacterial bioassays including two pl...

Defensive toxins are widespread in nature, yet we know little about how various environmental factors shape the evolution of chemical defense, especially in vertebrates. In this study we investigated the natural variation in the amount and composition of bufadienolide toxins, and the relative importance of ecological factors in predicting that vari...

Agaricus bisporus (button mushroom) is one of the most consumed edible mushrooms in the world. The components of its methanol and ethyl acetate extracts were tested for their ability to inhibit the growth of a soil bacterium Bacillus subtilis utilizing direct bioautography (thin-layer chromatography (TLC) coupled with bioassay). The active componen...

Bioassay guidance was used along the whole process including method development, isolation and identification of antibacterial neem (Azadirachta indica) oil compounds. The biomonitoring was performed by direct bioautography (DB), a combination of thin-layer chromatography (TLC) and antimicrobial detection. DB of neem oil showed one antibacterial zo...

King oyster mushroom (Pleurotus eryngii) is a worldwide cultivated mushroom of appreciated nutritional and medicinal quality. Aims of these investigations were to give new comparative data for the better evaluation of P. Eryngii. Results of our investigations can be summarized as follows: P. Eryngii has higher (crude protein, crude fat) or at least...

Background Data of mushroom nitrate content from scientific studies is limited. There have been two such recent investigations (mainly regarding certain cultivated species). To obtain comparable analytical data, we analyzed 134 samples of 54 taxa gathered and prepared by our Department.ResultsThe mushroom species were evaluated according to their n...

Summer truffle (Tuber aestivum Vittadini) is one of the most important mycorrhizal mushrooms with underground fruit bodies. Formerly the scientific investigations were focused mainly on its specific fragrant constituents. Our work was concentrated on complex chemical characterization including different organic and inorganic components. Summer truf...