Daniel KalinowskiUniversity of Warmia and Mazury in Olsztyn · Department of Animal Anatomy and Physiology
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In my research, I assess neurochemical changes in amygdala cell populations that cause increased anxiety and increased anxiety reactions in adult female mice with an estrogen receptor beta defect. Using immunohistochemistry techniques, I analyze key subpopulations of excitatory (glutaminergic) and inhibitors (GABAergic) neurons, providing basic balance in stimulation and inhibition processes. I research neurons primarily at the level of neurotransmitters and neuromodulators used.
Oestrogen receptor β (ERβ) knock-out female mice display increased anxiety and decreased threshold for synaptic plasticity induction in the basolateral amygdala. This may suggest that the γ-aminobutyric acid (GABA) inhibitory system is altered. Therefore, the immunoreactivity of main GABAergic markers—i.e., calbindin, parvalbumin, calretinin, somat...
The amygdala primarily evolved as a danger detector that regulates emotional behaviours and learning. However, it is also engaged in stress responses as well as olfactory/pheromonal and reproductive functions. All of these functions are processed by a set of nuclei which are derived from different telencephalic sources (pallial and subpallial) and...
The previous studies have shown that the female oestrogen receptor β (ERβ) knock-out mice display increased anxiety-like behaviours associated with a reduced threshold for the induction of synaptic plasticity in the basolateral amygdala. A reduced threshold in this brain region may indicate decreased γ-aminobutyric acid (GABA) activity that could l...
Introduction: Studies in oestrogen receptor β (ERβ) knock-out mice revealed significant hypocellularity in the amygdala. Moreover, mutant animals (especially females) displayed increased anxiety-like behaviours associated with a reduced threshold for the induction of synaptic plasticity in the amygdala suggesting a decreased GABA activity in this b...
Introduction: Pleomorphic adenoma gene-like 1 gene (PLAGL1, also known as ZAC1 or LOT1) encodes a zinc-finger transcription factor that was shown to induce cellcycle arrest and promote apoptosis. Deletion or hypermethylation of PLAGL1 was demonstrated in several types of human malignancies. Our previous studies did not support a tumor suppressor ro...
PLAGL1 is a transcription factor that was shown as anti-proliferative agent acting by cell cycle arrest and induction of apoptosis. Putative tumor suppressor role of PLAGL1 was evidenced in several human tumors. Previous studies on PLAGL1 in cancer cell carcinoma (ccRCC) did not support suppressive character of PLAGL1 in this malignancy. Therefore,...
The purpose of this study was to develop and test the protocol for in vitro transfection of renal cancer cell line Caki-2 with miRNA mimics. Micro-RNAs were delivered to the cells by lipofection using cationic lipid reagent Lipofectamine RNAiMAX. The cells were transfected with miRNA-1 or sc-miRNA that were used as positive or negative transfection...
I use 0.1% Sudan Black B in my brain immunofluorescence staining, even though it reduces autofluorescence of background but also gives low non-specific staining. It looks like neuronal marker staining.