Daniel H. Fine

Daniel H. Fine
Rutgers New Jersey Medical School | UMDNJ · Department of Oral Biology

DMD

About

307
Publications
23,636
Reads
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9,762
Citations
Additional affiliations
July 2013 - present
Rutgers School of Dental Medicine
Position
  • Professor and Chair, Senior Dean of Research Graduate School of Biomedical Sciences
Description
  • school is now called Rutgers School of Dental Medicine
January 1986 - July 1989
Columbia University
Position
  • Senior Research Fellow
July 1967 - July 1970
University of Pittsburgh
Position
  • Professor (Assistant)

Publications

Publications (307)
Article
Full-text available
Like the bacterial residents of the human gut, it is likely that many of the species in the human oral microbiota have evolved to better occupy and persist in their niche. Aggregatibacter actinomycetemcomitans (Aa) is both a common colonizer of the oral cavity and has been implicated in the pathogenesis of periodontal disease. Here, we present a wh...
Article
Full-text available
The significance of microbiology and immunology with regard to caries and periodontal disease gained substantial clinical or research consideration in the mid 1960's. This enhanced emphasis related to several simple but elegant experiments illustrating the relevance of bacteria to oral infections. Since that point, the understanding of oral disease...
Article
Full-text available
Severe acute respiratory syndrome-related coronavirus (SARS-CoV-2) is detectable in saliva from asymptomatic individuals, suggesting a potential benefit from the use of mouth rinses to suppress viral load and reduce virus spread. Published studies on the reduction of SARS-CoV-2-induced cytotoxic effects by mouth rinses do not exclude antiseptic mou...
Article
Full-text available
Aggregatibacter actinomycetemcomitans, the focus of this review, was initially proposed as a microbe directly related to a phenotypically distinct form of periodontitis called localized juvenile periodontitis. At the time, it seemed as if specific microbes were implicated as the cause of distinct forms of disease. Over the years, much has changed....
Article
Introduction. Candida albicans is responsible for several types of oral and systemic infections. In light of emerging resistance to antifungals, studies have demonstrated the antifungal effect of lactoferrin (LF), which is part of the innate immune system, has anticandidal activities.Methodology. C. albicans (2×106 c.f.u. ml-1) were incubated eithe...
Article
Full-text available
Aggregatibacter actinomycetemcomitans is associated with aggressive periodontitis resulting in premature tooth loss in adolescents. Tooth adherence and biofilm persistence are prerequisites for survival in the oral domain. Here, using a rhesus monkey model, 16S rRNA sequencing, and weighted network analysis, we assessed colonization of A. actinomyc...
Article
Full-text available
Objective: Fixed orthodontic appliances may influence the oral environment through accumulation of plaque, decreased plaque pH, and increased gingival inflammation. These changes in the oral cavity can potentially lead to periodontal disease, demineralization, and other infectious diseases. Materials and Methods: To investigate the changes in biofi...
Article
Objective: The objective of this study was to examine the relationship between iron deficiency and caries susceptibility in a mouse model. Materials and methods: Three-week-old C57BL/J6 mice were fed a cariogenic diet containing either standard iron (48 ppm Fe) or low iron (4 ppm Fe) levels. Concurrently, groups of mice with both diets were oral...
Article
Full-text available
Aggregatibacter actinomycetemcomitans (Aa) is a low-abundance Gram-negative oral pathobiont that is highly associated with a silent but aggressive orphan disease that results in periodontitis and tooth loss in adolescents of African heritage. For the most part Aa conducts its business by utilizing strategies allowing it to conceal itself below the...
Article
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A new periodontitis classification scheme has been adopted, in which forms of the disease previously recognized as “chronic” or “aggressive” are now grouped under a single category (“periodontitis”) and are further characterized based on a multi‐dimensional staging and grading system. Staging is largely dependent upon the severity of disease at pre...
Article
Full-text available
Objective: Since the initial description of aggressive periodontitis (AgP) in the early 1900s, classification of this disease has been in flux. The goal of this manuscript is to review the existing literature and to revisit definitions and diagnostic criteria for AgP. Study analysis: An extensive literature search was performed that included dat...
Article
Objective Since the initial description of aggressive periodontitis (AgP) in the early 1900s, classification of this disease has been in flux. The goal of this manuscript is to review the existing literature and to revisit definitions and diagnostic criteria for AgP. Study analysis An extensive literature search was performed that included databas...
Article
The oral cavity harbors different taxonomic groups, the evolutionary co‐existence of which develops the oral ecosystem. These resident microorganisms can alter the balance between the physiologic and pathologic conditions that affect the host, both locally and systemically. This highly sophisticated nature of the oral cavity poses a significant the...
Article
The Gram-negative bacterium Aggregatibacter actinomycetemcomitans is a causative agent of localized aggressive periodontitis. Critical to its infection process is the first and essential step of attachment, which is related to the coordinated functions of surface components comprised of proteins and extracellular polysaccharides. One such protein i...
Article
Aim: The aims of this study were to; 1) test susceptibility to bacteremia in subjects with moderate gingivitis, and 2) compare the effects of brushing with a fluoride toothpaste (control) as compared to a triclosan/copolymer toothpaste (test) on those susceptible to repeated bacteremia. Materials and methods: 107 adult subjects were tested for r...
Article
Experiments were designed to explore a prominent autoinducer-2 (AI-2) producing gene (luxS) related to colonization and survival of Aggregatibacter actinomycetemcomitans, a low abundance member of the indigenous flora, that forms a key component of the dysbiotic flora in localized aggressive periodontitis. The luxS gene was disrupted in a primate s...
Article
Full-text available
We report here the complete genomic sequence and methylome of Aggregatibacter actinomycetemcomitans strain IDH781. This rough strain is used extensively as a model organism to characterize localized aggressive periodontitis pathogenesis, the basic biology and oral cavity colonization of A. actinomycetemcomitans, and its interactions with other memb...
Article
Article Title and Bibliographic Information Host-derived salivary biomarkers in diagnosing periodontal disease: systematic review and meta-analysis. de Lima CL, Acevedo AC, Grisl DC, Taba M Jr, Guerra E, De Luca Canto G. J Clin Periodontol 2016; 43(6): 492-502. Source of Funding Self-funded Type of Study/Design Systematic review with meta-analysi...
Article
Full-text available
Leukotoxin (Ltx) is a prominent virulence factor produced by Aggregatibacter actinomycetemcomitans, an oral microorganism highly associated with aggressive periodontitis. Ltx compromises host responsiveness by altering the viability of neutrophils, lymphocytes, and macrophages. Previously, we developed a Rhesus (Rh) monkey colonization model design...
Article
The objective of this study is to detrmine whether alloxan-induced diabetic Lactoferrin knockout (LFKO(-/-)) mice are more susceptible to periodontal disease caused by Aggregatibacter actinomycetemcomitans compared to the diabetic wild-type (WT) mice. Diabetes was induced in mice by a single dose of alloxan (60 mg/kg) injected intravenously. Mice w...
Article
Objectives: The objective of this study is to evaluate the importance of human lactoferrin (hLF) in an experimental caries induced by Streptococcus mutans in a lactoferrin knockout (LFKO(-/-) ) mouse model compared with C576J/BL wild type (WT) mice. Materials and methods: The WT and LFKO(-/-) mice were infected with S. mutans (1×10(8) cells) and...
Article
Among the various proteins expressed by the periodontopathogen Aggregatibacter actinomycetemcomitans, two proteins play important roles for survival in the oral cavity. The autotransporter Aae facilitates the attachment of the pathogen to oral epithelial cells, which act as a reservoir, while the biofilm-degrading glycoside hydrolase dispersin B fa...
Article
Full-text available
Aggregatibacter actinomycetemcomitans (Aa) is a pathobiont and part of a consortium of bacteria that can lead to periodontitis in humans. Our aim was to develop a model for oral inoculation of labeled Aa into a suitable host in order to study Aa traits and ecological factors that either enhance or repress its persistence. Primate species were scree...
Article
Full-text available
Lactoferrin is one of a number of multifunctional proteins that are present in or on all mucosal surfaces throughout the body. Levels of lactoferrin are consistently elevated in inflammatory diseases such as arthritis, inflammatory bowel diseases, corneal disease, and periodontitis. Single-nucleotide polymorphisms (SNPs) in lactoferrin have been sh...
Conference Paper
LEUKOTOXIN EXPRESSION IN Aggregatibacter actinomycetemcomitans STRAIN DERIVED FROM MONKEY Objectives: We have developed a monkey model that permits us to study colonization of Rhesus-derived labeled Aggregatibacter actinomycetemcomitans (Aa) strain over a 4-week period. This model helps us understand the role of Aa's virulence factors in relation...
Conference Paper
Objectives: Aggregatibacter actinomycetemcomitans (Aa), a Gram-negative, periodonto-pathogen produces leukotoxin (Ltx) that can kill lymphocytes. Our previous non-human primate Aa colonization study showed that the ltxA knockout strain (ΔltxA) did not colonize the tooth surface of the monkeys compared to the wild-type (WT) strain. The objective of...
Article
Full-text available
Aggregatibacter actinomycetemcomitans a causative agent of periodontal disease in humans, forms biofilm on biotic and abiotic surfaces. A. actinomycetemcomitans biofilm is heterogeneous in nature and is composed of proteins, extracellular DNA and exopolysaccharide. To explore the role played by the exopolysaccharide in the colonization and disease...
Article
Full-text available
Leukocyte Adhesion Deficiency I (LAD-I) is a primary immunodeficiency caused by single gene mutations in the CD18 subunit of β2 integrins which result in defective transmigration of neutrophils into the tissues. Affected patients suffer from recurrent life threatening infections and severe oral disease (periodontitis). Microbial communities in the...
Article
Aggregatibacter actinomycetemcomitans a causative agent of periodontal disease in humans , forms biofilm on biotic and abiotic surfaces. A. actinomycetemcomitans biofilm is heterogeneous in nature and is composed of proteins, extracellular DNA and exopolysac-charide. To explore the role played by the exopolysaccharide in the colonization and diseas...
Article
AimsTo determine the role of lactoferrin in protecting the oral cavities of mice against Candida albicans infection in lactoferrin knockout (LFKO-/-) mice were compared to wild-type (WT) mice. We also determine the protective role of human lactoferrin in the LFKO-/- mice.Methods and ResultsAntibiotic treated immunosuppressed mice were inoculated wi...
Article
Streptococcus mutans is the primary agent of dental caries, which is often detected in transient bacteremia. Lactoferrin is a multifunctional glycoprotein showing antibacterial activities against several Streptococcus species. We reported here the prophylactic effect of human lactoferrin (hLF) in a lactoferrin knockout mouse (LFKO(-/-)) bacteremic...
Conference Paper
Objective: This cross-over design clinical study compared whole mouth antimicrobial effects of two oral hygiene regimens 12-hours after twice-daily use. The regimens were (i) Test: brushing with 0.3% triclosan/copolymer toothpaste, rinsing with a 0.075% Cetylpyridinium chloride (CPC) mouthwash and a toothbrush with tongue-cleaner (Colgate 360 too...
Article
Full-text available
Improved diagnostics remains a fundamental goal of biomedical research. This study was designed to assess cytokine biomarkers that could predict bone loss (BL) in localized aggressive periodontitis. 2,058 adolescents were screened. Two groups of 50 periodontally healthy adolescents were enrolled in the longitudinal study. One group had Aggregatibac...
Conference Paper
Full-text available
Objective: Aggregatibacter actinomycetemcomitans is a gram-negative bacterium that is present in the oral cavity of humans and primates. We have isolated several strains of Aa from Rhesus monkeys and the genome sequence of monkey Aa revealed the presence of all virulence factors including leukotoxin. However, so far, there has been no report on gen...
Conference Paper
Objective: Aggregatibacter actinomycetemcomitans (Aa), a Gram-negative, facultative anaerobe is considered to be the etiological agent of localized aggressive periodontitis (LAP). The LuxS of Aa is responsible for its growth under iron limiting conditions. The objective of the study is to determine the effect of LuxS that affects community organiza...
Conference Paper
Objective: Our goal is to develop a primate model that can assess colonization and persistence of an antibiotic labeled Aggregatibacter actinomycetemcomitans (Aa) strain for in vivo analysis. Methods: Initially we surveyed 65 Old World and 35 New World primates for the presence of Aa. We compared colonization and persistence two Aa strains; one der...
Article
Periodontal disease is the most common osteolytic disease in humans and is significantly increased by diabetes mellitus. We tested the hypothesis that bacterial infection induces bone loss in diabetic animals through a mechanism that involves enhanced apoptosis. Type II diabetic rats were inoculated with Aggregatibacter actinomycetemcomitans and tr...
Article
Full-text available
Aggregatibacter actinomycetemcomitans, a periodontopathogen, has been associated with several systemic diseases. Herein, we report the protective effect of human lactoferrin (hLF) during A. actinomycetemcomitans bacteremia in lactoferrin knockout (LFKO−/−) mice. The prophylactic, concurrent, and therapeutic intravenous (i.v.) administrations of hLF...
Article
Full-text available
Periodontal disease is the most common osteolytic disease in humans and is significantly increased by diabetes mellitus. We tested the hypothesis that bacterial infection induces bone loss in diabetic animals through a mechanism that involves enhanced apoptosis. Type II diabetic rats were inoculated with Aggregatibacter actinomycetemcomitans and tr...
Article
The objectives of this paper are to present an overview of children's oral health-related quality of life and include specific applications for using quality of life assessment in dental research. The process of developing pediatric oral health- related quality of life measures, in particular the Child Oral Health Impact Profile, is outlined. Examp...
Patent
The present invention relates generally to dental diseases, caries and periodontal disease. More specifically, the invention relates to Lactoferrin and Statherin fusion proteins (STAT-LF) along with therapeutic, diagnostic and research uses for these polypeptides. The present invention also provides methods of treating dental diseases, caries and p...
Article
Full-text available
Our aim was to explore the effects of Cytolethal Distending toxin (Cdt) in a well established rat model of periodontal disease where leukotoxin (LtxA) was thought to have no known effect. In vitro studies, were used to assess CdtB activity using Aa Leukotoxin as a negative control. These studies showed that both CdtB and LtxA (unexpectedly) exerted...
Article
Full-text available
Aggregatibacter actinomycetemcomitans-induced localized aggressive periodontitis (LAP) in African-American adolescents has been documented but is poorly understood. Two thousand fifty-eight adolescents aged 11 to 17 years were screened for their periodontal status and the presence of A. actinomycetemcomitans in their oral cavity. Seventy-one A. act...
Conference Paper
Objective: 2,058 children ages 11-17 years were screened for the presence of Aggregatibacter actinomycetemcomitans (Aa) and localized aggressive periodontitis (LAP). Goals were to determine; 1) the relationship of Aa to BL, and 2) the relationship of Aa to other subgingival bacteria. Methods: 134 periodontally healthy children (73 Aa-negative and...
Conference Paper
Objective: : This clinical study compared the effects of toothbrushing in conjunction with rinsing with a mouthrinse formulated with a 0.075% cetylpyridinium chloride (CPC) to toothbrushing alone on anaerobic plaque bacteria. Method: A cross-over design study enrolled 50 adults (average age 36.6, age range: 18-59 years). Subjects completed a 1-we...
Conference Paper
Objective: Children 11-17 years old were screened for localized aggressive periodontitis (LAP) and the presence of Aggregatibacter actinomycetemcomitans (Aa). Goals were to determine; 1)salivary factors related to microbial colonization and BL, and 2) crevicular cytokines in relation to BL. Methods: 134 periodontally healthy children (73 Aa-negat...
Conference Paper
Objective: Lactoferrin, a multifunctional protein plays an important role in protecting against oral and systemic infections caused by various microorganisms. To understand the essential role of lactoferrin during A. actinomycetemcomitans (Aa) systemic infection, Lactoferrin Knockout (LFKO-/-) mice were compared with add back of LF in an experiment...
Conference Paper
Objective: The salivary protein lactotransferrin (LTF) has been implicated in infectious diseases, including periodontitis. The objective of this research was to advance understanding of LTF Single Nucleotide Polymorphisms (SNPs) for susceptibility to localized and generalized early-onset aggressive and chronic periodontitis (AgP and CP). Methods...
Article
Full-text available
Streptococcus mutans is prominently linked to dental caries. Saliva's influence on caries is incompletely understood. Our goal was to identify a salivary protein with anti-S. mutans activity, characterize its genotype, and determine genotypic variants associated with S. mutans activity and reduced caries. An S. mutans affinity column was used to is...
Article
Full-text available
Background: Among the innate defense mechanisms in the oral cavity, lactoferrin (LF) is a vital antimicrobial that can modify the host response against periodontopathogens. Aggregatibacter actinomycetemcomitans is the main periodontopathogen of localized aggressive periodontitis. The aim of this study is to evaluate the role of LF during A. actino...
Article
Full-text available
Objective—Dental caries is a significant public health problem especially amongst children from low-income backgrounds. This longitudinal study examined the development of new occlusal caries in 227 Newark, NJ children ages 10–18. The role of previous caries experience and the presence of occlusal white and dark lesions in predicting the developmen...
Article
This study compared the antimicrobial effects of three commercial dentifrice formulations: sodium fluoride/triclosan/copolymer (TCN/C), stannous fluoride/sodium hexametaphosphate/zinc lactate (SnF(2) /SHMP) and sodium fluoride (NaF). Thirty-five adults (15 men and 20 women; average age 33 years and pockets <5 mm) completed this double-blind, triple...
Article
Objective: Dental caries is a significant public health problem especially amongst children from low-income backgrounds. This longitudinal study examined the development of new occlusal caries in 227 Newark, NJ children ages 10-18. The role of previous caries experience and the presence of occlusal white and dark lesions in predicting the developm...
Article
Healthy subjects who do not have Aggregatibacter actinomycetemcomitans in their oral cavity may possess factors in saliva that might demonstrate antibacterial activity against the bacterium. The aim of this study was to identify and purify proteins from saliva of healthy subjects that might demonstrate antibacterial activity against A. actinomycete...
Article
Full-text available
The purpose of this study was to test the hypothesis that diabetes aggravates periodontal destruction induced by Aggregatibacter actinomycetemcomitans infection. Thirty-eight diabetic and 33 normal rats were inoculated with A. actinomycetemcomitans and euthanized at baseline and at 4, 5, and 6 weeks after inoculation. Bone loss and the infiltration...
Conference Paper
Objectives: Lactoferrin (Lf) is an iron-binding 80-kDa glycoprotein found in milk, mucosal secretion and neutrophil secondary granules with antimicrobial and immunomodulatory activities. To understand the significance of Lf in periodontal disease, susceptibility of wild type and Lf knockout (Lf-/-) mice were compared in an experimental periodontal...