Daniel Eklund

Daniel Eklund
Örebro University | oru · School of Medical Sciences

PhD

About

43
Publications
4,016
Reads
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588
Citations
Citations since 2017
20 Research Items
391 Citations
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080
Additional affiliations
November 2018 - present
Örebro University
Position
  • Lecturer
January 2017 - October 2018
Örebro University
Position
  • Professor (Assistant)
January 2014 - present
Linköping University
Position
  • Research Associate
Education
August 2003 - May 2008
Linköping University
Field of study
  • Biomedicine

Publications

Publications (43)
Article
In this explorative study, we investigated if an adjunctive treatment with one single dose of the monoclonal antibody rituximab would improve symptoms and function in treatment-resistant patients with schizophrenia spectrum disorder (SSD, n = 9) or obsessive-compulsive disorder (OCD, n = 10), based on the inflammatory hypothesis for mental disorder...
Article
Full-text available
Myeloid‐derived suppressor cells (MDSCs) are functionally immunosuppressive cells that arise and expand during extensive inflammatory conditions by increased hematopoietic output or reprogramming of immune cells. In sepsis, an increase of circulating MDSCs is associated with adverse outcomes, but unique traits that can be used to identify increased...
Article
Full-text available
Introduction In light of potential negative health effects of cobalt exposure, a characterization of inflammatory mechanisms in exposed individuals is warranted. The current study investigated cobalt exposure in the Swedish hard metal industry and its relationship to inflammatory markers, including NLRP3 inflammasome activation and white blood cell...
Article
Full-text available
Mental disorders are heterogeneous and psychiatric comorbidities are common. Previous studies have suggested a link between inflammation and mental disorders. This link can manifest as increased levels of proinflammatory mediators in circulation and as signs of neuroinflammation. Furthermore, there is strong evidence that individuals suffering from...
Article
Full-text available
Introduction Leptin signaling in placentas of obese women may influence fetal growth and may be dependent on fetal sex. The aim of this study was to investigate placental gene expression of leptin, its receptor and inflammatory cytokines in obese mothers in relation to offspring birth weight and sex. Methods In total, 109 placental tissue samples...
Article
Full-text available
Both initiation and suppression of inflammation are hallmarks of the immune response. If not balanced, the inflammation may cause extensive tissue damage, which is associated with common diseases, e.g., asthma and atherosclerosis. Anti-inflammatory drugs come with side effects that may be aggravated by high and fluctuating drug concentrations. To r...
Article
Full-text available
Inflammasomes cleave and activate interleukin- (IL-) 1β and IL-18 which have both shared and unique biological functions. IL-1β is an important mediator of the acute phase response to infections and tissue damage, whereas IL-18 takes part in activation and tailoring of the adaptive immune response. While IL-1β has served as the prototypic indicator...
Preprint
Full-text available
Both initiation and suppression of inflammation are hallmarks of the immune response. If not balanced, the inflammation may cause extensive tissue damage, which is associated with common diseases, e.g. asthma and atherosclerosis. Anti-inflammatory drugs often come with severe side effects driven by high and fluctuating drug concentrations. To remed...
Article
Full-text available
Purpose: To study the association between inhalation of particulate matter or quartz in Swedish iron foundries and the effects on NLRP3 inflammasome activation. Methods: Particle exposure measurements were performed during an eight-hour work day for 85 foundry workers at three Swedish iron foundries. Personal sampling was used for measurement of...
Article
Full-text available
The aim of this study was to investigate inflammatory perturbations in 40 patients with severe and complex psychiatric disorders by studying the activity of the NLRP3 inflammasome, with a trans-diagnostic approach. Gene expression of CASP1, NLRP3, PYCARD, IL1B, IL1RN, TNF showed a significant increase in the patient group compared to a matched cont...
Article
To prospectively study systemic in vivo immunological effects of sex hormones, using different phases of oral combined hormonal contraceptives (CHC), and the natural menstrual cycles in both healthy women and in women with multiple sclerosis (MS), blood samples from sixty female MS patients and healthy controls with and without CHC were drawn in hi...
Article
Full-text available
Background Drugs such as isoniazid (INH) and pretomanid (PRT), used against Mycobacterium tuberculosis are active partly through generation of reactive nitrogen species (RNS). The aim of this study was to explore variability in intracellular susceptibility to nitric oxide (NO) in clinical strains of M. tuberculosis. Method Luciferase-expressing cl...
Data
Bacterial load in broth cultures exposed to DETA/NO and 1mM SIN-1. The H37Rv and BTB 02–141 were exposed to 0.1mM DETA/NO (a) and 1mM SIN-1 (b). Bacterial load after 4hours, 24h and 4 days later was determined by luminometry. Data are presented as mean bacterial survival (% of unexposed control) ± SEM, (n = 4–5). Significant differences from H37Rv...
Data
The nitrite level in supernatants of activated macrophages after treatment with L-NMMA. Macrophages (RAW 264.7) were stimulated with IFN-γ/LPS alone or in combination with 1mM iNOS inhibitor L-NMMA. Nitrite levels were measured with the Griess assay. The lowest concentration within the linear range of the standard curve was considered as detection...
Data
Intracellular survival of M tuberculosis clinical isolates. Macrophages (RAW 264.7) were stimulated with IFN-γ/LPS and/or the iNOS inhibitor L-NMMA (1mM) or left untreated. Macrophages were infected with H37Rv (a), the INH-susceptible strains E3942 (b), CDC 1551 (c), the INH-resistant strains BTB 02–141 (d), and E1155 (e) at a MOI of 5. Bacterial l...
Data
Growth in brothof different strains of M. tuberculosis. The strains were cultured in Middlebrook 7H9 medium supplemented with Tween 80 and ADC and bacterial load was determined by luminometry. Data are presented as mean log10 of fold change in bacterial numbers between day 4 and day 0 (log 10 of D4/D0), (n≥3). Significant differences from H37Rv is...
Data
Phagocytosis of clinical strains. Macrophages (RAW 264.7) were stimulated with IFN-γ/LPS and/or iNOS inhibitor L-NMMA or left untreated. Phagocytosis of the reference strain H37Rv (a), the INH-susceptible strains E3942/94 Beijing (b), CDC 1551 (c) the two INH-resistant strains BTB 02–141 (d) and E1155/94 (e) by macrophages was measured by determini...
Data
Dose dependent effects of DETA/NO and SIN-1 against M. tuberculosis. The bacterial strains were exposed to increasing concentrations of DETA/NO (a) and SIN-1 (b) and bacterial numbers weres determined by luminometry. Data are presented as mean bacterial survival (% of unexposed control) after 4 days of incubation ± SEM, (n = 3). Significant differe...
Article
Full-text available
Multiple sclerosis (MS) is a chronic inflammatory disease of the CNS and has a varying disease course as well as variable response to treatment. Biomarkers may therefore aid personalized treatment. We tested whether in vitro activation of MS patient-derived CD4+ T cells could reveal potential biomarkers. The dynamic gene expression response to acti...
Article
Full-text available
Early regulators of disease may increase understanding of disease mechanisms and serve as markers for presymptomatic diagnosis and treatment. However, early regulators are difficult to identify because patients generally present after they are symptomatic. We hypothesized that early regulators of T cell-associated diseases could be found by identif...
Article
Full-text available
The standard treatment of tuberculosis (TB) takes six to nine months to complete and this lengthy therapy contributes to the emergence of drug-resistant TB. TB is caused by Mycobacterium tuberculosis (Mtb) and the ability of this bacterium to switch to a dormant phenotype has been suggested to be responsible for the slow clearance during treatment....
Article
Full-text available
Macrophages in the lung are the primary cells being infected by Mycobacterium tuberculosis (Mtb) during the initial manifestation of tuberculosis. Since the adaptive immune response to Mtb is delayed, innate immune cells such as macrophages and neutrophils mount the early immune protection against this intracellular pathogen. Neutrophils are short-...
Thesis
Full-text available
Mycobacterium tuberculosis is a very successful pathogen and tuberculosis constitutes a major threat to global health worldwide. The World Health Organization (WHO) estimates that almost nine million new cases and 1.5 million deaths occur annually and the situation is worsened by increased antibiotic resistance and an extreme synergism with the HIV...
Article
Full-text available
Activation of the NLRP3 inflammasome and subsequent generation of interleukin 1β is initiated in macrophages upon recognition of several stimuli. In the present work, we show that gain-of-function gene variants of inflammasome components known to predispose individuals to inflammatory disorders have a host-protective role during infection with Myco...
Article
Full-text available
The emergence of multidrug-resistant strains of Mycobacterium tuberculosis (MTB), the bacterium responsible for tuberculosis (TB), has rekindled the interest in the role of nutritional supplementation of micronutrients, such as vitamin D, as adjuvant treatment. Here, the growth of virulent MTB in macrophages obtained from the peripheral blood of pa...
Article
Full-text available
Background: The role of vitamin D supplementation as adjuvant treatment of tuberculosis (TB) has lately attracted increasing interest. Our aim was to investigate the capacity of alveolar macrophage ...
Data
Similarity in growth between Mtb strains, and correlation between viable count and luciferase activity upon phagocytosis. A) 7H9 broth was inoculated with the different strains and the increase in bacterial numbers was assessed by OD600 at the indicated time points. The OD value was normalized against the Day 0 value for each time point and strain...
Data
Increased HMGB1 release upon H37Rv infection is independent of caspase-1 and cathepsin B, but dependent on an intact ESAT-6 gene. hMDMs treated with DMSO (1:1000), YVAD, or CA-074Me were infected with H37Rv for two days at MOI 10. Alternatively, hMDMs were left uninfected or were infected at MOI 10 for two days with H37Rv or an ESAT-6 deletion muta...
Data
YVAD, CA-074Me and ZVAD do not affect macrophage viability. hMDMs were left untreated or were treated for 24 h with DMSO (1:1000) or the inhibitors at the concentration used in the manuscript. Three different staining procedures were used to investigate cell death features, and the cells were analyzed by flow cytometry. A) TUNEL analysis of DNA fra...
Data
Fluorescence microscopy images of TUNEL-stained hMDMs. hMDMs on glass cover slips were infected with H37Ra or H37Rv at MOI 10, or left uninfected, fixed after two days of infection, and fragmented DNA was visualized by TUNEL staining. As a positive control, the hMDMs were treated with DNase prior to staining. Images were acquired using a fluorescen...
Data
ZVAD and CA-074Me, but not YVAD, reduce UV- or staurosporine-induced apoptosis. hMDMs were treated with DMSO (1:1000) or inhibitors at the indicated concentration, and apoptosis was induced by UV light or by treatment with staurosporine for 18 h at the indicated concentration. Apoptosis was then assessed by TUNEL or Annexin V staining. A) Microscop...
Article
Full-text available
Mycobacterium tuberculosis (Mtb) infects lung macrophages, which instead of killing the pathogen can be manipulated by the bacilli, creating an environment suitable for intracellular replication and spread to adjacent cells. The role of host cell death during Mtb infection is debated because the bacilli have been shown to be both anti-apoptotic, ke...
Article
Full-text available
The best characterized survival mechanism of Mycobacterium tuberculosis inside the macrophage is the inhibition of phagosomal maturation. Phagosomal maturation involves several steps including fusion with lysosomes and acidification. However, it has not been elucidated which components of phagosomal maturation correlate with growth restriction of v...
Article
Full-text available
Intracellular pathogens such as Mycobacterium tuberculosis have adapted to a life inside host cells, in which they utilize host nutrients to replicate and spread. Ineffective methods for the evaluation of growth of intracellular pathogens in their true environment pose an obstacle for basic research and drug screening. Here we present the validatio...
Article
Modulation of immune cell apoptosis is a key evasion strategy utilized by Mycobacterium tuberculosis (Mtb). To be able to multiply within macrophages, the bacterium delays apoptosis and down-regulates pro-inflammatory activation in these cells, whereas apoptosis is rapidly induced in the potently bactericidal neutrophils. Initial host-pathogen inte...

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Project (1)
Project
Studying the connection between inflammation and severe mental illness.