Dana N Levine

Dana N Levine
Iowa State University | ISU · Department of Veterinary Clinical Sciences

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56
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Introduction

Publications

Publications (56)
Chapter
Immune thrombocytopenia (ITP) is a common bleeding disorder in which autoimmune destruction of platelets and megakaryocytes results in severe thrombocytopenia. This chapter focuses on ITP in dogs, the veterinary species in which it is most prevalent, and notes relevant differences in other species. Information from human ITP is referenced for knowl...
Article
Background: Canine immune thrombocytopenia (ITP) ranges from a mild to severe bleeding disorder, and platelet counts do not reliably predict clinical disease course. The detection of platelet autoantibodies may further define the disease phenotype, but variability in assay configurations and a lack of well-characterized controls limit the diagnost...
Article
Full-text available
Background Vincristine might increase circulating platelet numbers but the functional capacity of these newly released platelets is unknown. Objective To evaluate and compare the functionality of mature and immature (reticulated) platelets after a single intravenous dose of vincristine in dogs. Animals Ten healthy purpose‐bred dogs. Methods Dogs...
Article
Full-text available
Background: Thrombocytopenia in dogs is common in critical care medicine, but availability of fresh platelet concentrates in veterinary medicine can be limiting. Lyophilized platelets have long shelf-lives and can be easily transported, stored, and administered in various settings. Objective: To evaluate the efficacy and safety of a novel trehal...
Article
Full-text available
Background: Neutrophil extracellular traps (NETs), webs of DNA and citrullinated histones extruded from activated neutrophils cause transfusion-related acute lung injury. Supernatants of stored red blood cell (RBC) units might promote NETosis in neutrophils from the units or from transfusion recipients. Hypotheses: (1) NETs form during storage o...
Article
Objective: To evaluate the performances of a manual Nageotte hemocytometer method and commercial fluorescent bead-based flow cytometric assay for quantifying [rWBC] in leukoreduced canine packed red blood cell (pRBC) units. Design: Prospective study. Five, commercially purchased, double leukoreduced canine pRBC units were spiked with canine leuk...
Article
Background: Point-of-care (POC) portable blood glucose meters (PBGMs) are convenient and inexpensive tools for assessing patient blood glucose concentrations. They are often used to quickly diagnose hypoglycemia or collect serial glucose readings in diabetic patients. However, POC meters have been previously identified in human and veterinary lite...
Article
Immune thrombocytopenia (ITP) is a common autoimmune bleeding disorder. The understanding of ITP pathogenesis is rapidly evolving. We now recognize ITP as a complex and heterogeneous syndrome that results from a combination of humoral and cell‐mediated attacks on platelets peripherally and megakaryocytes in the bone marrow. Autoantibody‐mediated IT...
Article
Full-text available
Immune‐mediated hemolytic anemia (IMHA) causes severe anemia in dogs and is associated with considerable morbidity and mortality. Treatment with various immunosuppressive and antithrombotic drugs has been described anecdotally and in previous studies, but little consensus exists among veterinarians as to the optimal regimen to employ and maintain a...
Article
Full-text available
Immune‐mediated hemolytic anemia (IMHA) is an important cause of morbidity and mortality in dogs. IMHA also occurs in cats, although less commonly. IMHA is considered secondary when it can be attributed to an underlying disease, and as primary (idiopathic) if no cause is found. Eliminating diseases that cause IMHA may attenuate or stop immune‐media...
Article
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Thymoma-associated nephropathies have been reported in people but not in dogs. In this report, we describe a dog with thymoma and concurrent renal amyloidosis. A 7-year-old castrated male Weimaraner was presented for progressive anorexia, lethargy, and tachypnea. The dog was diagnosed with azotemia, marked proteinuria, and a thymoma that was surgic...
Data
Figure S1. Complete training course for correct implementation of the canine daily bleeding score assessment tool, DOGiBAT.
Data
Figure S5. Score sheet to record daily bleeding scores for clinical cases of ITP using the DOGiBAT.
Data
Table S1. Correlation data between clinical outcomes and admission DOGiBAT scores at individual anatomic sites in all enrolled dogs (n = 61). Spearman correlation coefficients (rs) and p‐values considered significant (p < 0.05) are in bolded text. Table S2. Correlation data between clinical outcomes and admission DOGiBAT scores at individual anato...
Data
Figure S2. Complete case‐based quiz utilized to assess correct implementation of the canine daily bleeding score assessment tool, DOGiBAT.
Data
Figure S3. Score sheet to record answers of the DOGiBAT quiz.
Data
Figure S4. Answer key for the DOGiBAT quiz.
Article
Bleeding heterogeneity amongst patients with immune thrombocytopenia (ITP) is poorly understood. Platelets play a role in maintaining endothelial integrity, and variable thrombocytopenia-induced endothelial changes may influence bleeding severity. Platelet-derived endothelial stabilizers and markers of endothelial integrity in ITP are largely under...
Article
Canine immune thrombocytopenia (ITP), unlike mouse models of induced anti-platelet immunity, occurs spontaneously in non-inbred populations. Canine ITP demonstrates the same spectrum of bleeding severity and variable response to front-line therapy as the human disease counterpart. Pet dogs and their owners live in close contact in a shared environm...
Article
Pet dogs experience similar environmental influences to their owners, and thus provide valuable spontaneous models for investigation of novel therapies for human disease. DNase and treatments targeting neutrophil extracellular trap (NET) proteins show promise in murine thrombosis models. Dogs at risk of thrombosis could provide evidence for the eff...
Article
Thrombosis is a major cause of mortality and morbidity in humans and dogs; however, anti-thrombotic drugs carry a risk of bleeding and increase the cost of patient care. The ability to identify individuals at high risk of thrombosis would allow targeting of anti-coagulant therapy at those most likely to derive a net benefit. Significant advances ha...
Article
Neutrophil extracellular traps (NETs) are webs of DNA and protein with both anti-microbial and pro-thrombotic properties which have not been previously reported in dogs. To confirm dog neutrophils can form NETs, neutrophils were isolated from healthy dogs, and stimulated in vitro with 2μM, 8μM, 31μM, and 125μM platelet activating factor (PAF) or 0....
Article
Full-text available
Sphingosine-1-phosphate (S1P) is a key biolipid signaling molecule that regulates cell growth and survival, but it has not been studied in tumors from dogs. S1P/S1P1 signaling will contribute to the progression of hemangiosarcoma (HSA). Thirteen spontaneous HSA tissues, 9 HSA cell lines, 8 nonmalignant tissues, including 6 splenic hematomas and 2 l...
Article
The aim of this study was to develop and prospectively validate a simple endoscopic score of disease activity for dogs with inflammatory bowel disease (IBD). Archived endoscopic still images and video recordings of gastric, duodenal, and colonic endoscopic examinations were displayed to novice and experienced endoscopists for assessment of inflamma...
Data
Fig S1. Effect of S1P on cell proliferation in serum‐deprived medium.
Data
Table S1. Primer sequences for qRT‐PCR.
Article
Background Endoscopy is performed for direct inspection of the mucosa and acquisition of biopsies in dogs with inflammatory bowel disease (IBD).AimTo evaluate the interobserver agreement in the endoscopic assessment of duodenal mucosa in dogs with IBD.Methods Thirty-five archived endoscopic images of grossly normal (n = 6) and inflamed (n = 29) duo...
Article
Canine immune thrombocytopenia (ITP) is analogous to human ITP, with similar platelet counts and heterogeneity in bleeding phenotype among affected individuals. With a goal of ultimately investigating this bleeding heterogeneity, a canine model of antibody-mediated ITP was developed. Infusion of healthy dogs with 2F9, a murine IgG2a monoclonal anti...
Article
2193 In both canine and human patients with Immune Thrombocytopenia (ITP), bleeding risk is challenging to predict, and potentially leads to over-treatment of patients at low risk. Conversely, recent studies have highlighted the risk of thrombosis in ITP during platelet recovery. Given these clinical observations, we hypothesized that in ITP, chang...
Article
3326 Immune thrombocytopenia (ITP) is a relatively prevalent disease in dogs with significant morbidity and mortality. Canine ITP is clinically analogous to human ITP, with heterogeneity in bleeding manifestations in individuals with similar platelet counts. With a view to ultimately investigate this bleeding heterogeneity, we set out to develop a...
Article
Full-text available
Ronidazole (RDZ) is the only known effective treatment for feline diarrhea caused by Tritrichomonas foetus. This study aimed to develop guar gum-coated colon-targeted tablets of RDZ and to determine the pharmacokinetics of this delayed-release formulation in cats. Guar gum-coated tablets were administered orally once to five healthy cats (mean dose...
Article
Full-text available
Ronidazole (RDZ) is an effective treatment for feline Tritrichomonas foetus infection, but has produced neurotoxicity in some cats. An understanding of the disposition of RDZ in cats is needed in order to make precise dosing recommendations. Single-dose pharmacokinetics of intravenous (IV) RDZ and immediate-release RDZ capsules were evaluated. A si...
Article
Evaluation of serial urine protein:creatinine (UPC) ratios is important in prognosticating chronic kidney disease and monitoring response to therapeutic interventions. Owing to random biologic variation in dogs with stable glomerular proteinuria, multiple determinations of UPC ratios often are recommended to reliably assess urine protein loss. This...
Article
Full-text available
Human papillomavirus (HPV) vaccines have the potential to prevent cervical cancer by preventing HPV infection or treating premalignant disease. We previously showed that DNA vaccination with the cottontail rabbit papillomavirus (CRPV) E6 gene induced partial protection against CRPV challenge and that the vaccine's effects were greatly enhanced by p...
Article
Senior seminar (D.V.M.)--Cornell University, 2004. Includes bibliographical references (leaf 19).

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