Damian KołatMedical University of Łódź | UMED · Zakład Kancerogenezy Molekularnej
Damian Kołat
PhD Student
About
37
Publications
3,140
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125
Citations
Citations since 2017
Introduction
I am a PhD student at the Medical University of Lodz and a co-author of student R&D project Farm@Bio. I'm passionate about molecular biology and genetic engineering as well as things related to computer modifications (hardware & software). I want to broaden my horizons as a biotechnologist or bioinformatics in order to be able to improve my research skills and work with the latest technological solutions. My most recent publication is 'Exosomes as carriers transporting long non‑coding RNAs: Molecular characteristics and their function in cancer', however daily I'm focused on research of AP-2 transcription factors and collaboration with WWOX gene.
Skills and Expertise
Publications
Publications (37)
Continuous monitoring of the population’s health is the main method of learning about disease prevalence. National and international data draw attention to the persistently high rates of cancer incidence. This necessitates the intensification of efforts aimed at developing new, more effective chemotherapeutic and chemopreventive drugs. Plants repre...
Following the discovery of WWOX, research has moved in many directions, including the role of this putative tumor suppressor in the central nervous system and related diseases. The task of determining the nature of WWOX in glioblastoma (GBM) is still considered to be at the initial stage; however, the influence of this gene on the GBM malignant phe...
Doxorubicin (DOX) constitutes the major constituent of anti-cancer treatment regimens currently in clinical use. However, the precise mechanisms of DOX’s action are not fully understood. Emerging evidence points to the pleiotropic anticancer activity of DOX, including its contribution to DNA damage, reactive oxygen species (ROS) production, apoptos...
The application of immunotherapy for cancer treatment is rapidly becoming more widespread. Immunotherapeutic agents are frequently combined with various types of treatments to obtain a more durable antitumor clinical response in patients who have developed resistance to monotherapy. Chemotherapeutic drugs that induce DNA damage and trigger DNA dama...
Hydrogels have the properties of solid substances and are useful for medicine, e.g., in systems for the controlled release of drugs or as wound dressings. They isolate the wound from the external environment and constitute a barrier to microorganisms while still being permeable to oxygen. In the current study, hydrogels were formed from concentrate...
Formerly hailed as “undruggable” proteins, transcription factors (TFs) are now under investigation for targeted therapy. In cancer, this may alter, inter alia, immune evasion or replicative immortality, which are implicated in genome organization, a process that accompanies multi-step tumorigenesis and which frequently develops in a non-random mann...
![Fig. 1. The prevalence of five waves of COVID-19 in Polish population [25].](publication/366207402/figure/fig1/AS:11431281108262716@1671423690494/The-prevalence-of-five-waves-of-COVID-19-in-Polish-population-25_Q320.jpg)
Background
Older individuals tend to include less physical activity in their routine and are more prone to chronic diseases and severe medical complications, making them the most burdened group that is losing years of life due to pandemic-related premature mortality.
This research aimed to assess the lifestyle factors that affect the COVID-19 cours...
Despite recovering from the acute phase of coronavirus disease (COVID-19), many patients report continuing symptoms that most commonly include fatigue, cough, neurologic problems, hair loss, headache, and musculoskeletal pain, a condition termed long-COVID syndrome. Neither its etiopathogenesis, nor its clinical presentation or risk factors are ful...
Transcription factors (TFs) constitute a wide and highly diverse group of proteins capable of controlling gene expression. Their roles in oncogenesis, tumor progression, and metastasis have been established, but recently their role in the DNA damage response pathway (DDR) has emerged. Many of them can affect elements of canonical DDR pathways, modu...
Malignancies dependent on hormone homeostasis include breast, ovary, cervical, prostate, testis and uterine tumors. Hormones are involved in signal transduction which orchestrate processes, such as apoptosis, proliferation, cell cycle or cytoskeleton organization. Currently, there is a need for novel biomarkers which would help to diagnose cancers...
The severity of ailments caused by SARS-CoV-2 varies and the clinical picture has already evolved during the pandemic, complicating diagnostics. In Poland, no study has been performed to assess the clinical picture of patients across the successive pandemic waves. The aim of the study was to present the characteristics of patients who present to me...
Following the invention of high-throughput sequencing, cancer research focused on investigating disease-related alterations, often inadvertently omitting tumor heterogeneity. This research was intended to limit the impact of heterogeneity on conclusions related to WWOX/AP-2α/AP-2γ in bladder cancer which differently influenced carcinogenesis. The s...
Identifying genes with prognostic importance could improve cancer treatment. An increasing number of reports suggest the existence of successful strategies based on seemingly “untargetable” transcription factors. In addition to embryogenesis, AP-2 transcription factors are known to play crucial roles in cancer development. Members of this family ca...
[This corrects the article DOI: 10.3389/fonc.2021.621060.].
The cytogenic locus of the WWOX gene overlaps with the second most active fragile site, FRA16D, which is present at a higher frequency in bladder cancer (BLCA) patients with smoking habit, a known risk factor of this tumor. Recently, we demonstrated the relevance of the role of WWOX in grade 2 BLCA in collaboration with two AP-2 transcription facto...
Glioblastoma is one of the deadliest human cancers. Its malignancy depends on cytoskeleton reorganization, which is related to, e.g., epithelial-to-mesenchymal transition and metastasis. The malignant phenotype of glioblastoma is also affected by the WWOX gene, which is lost in nearly a quarter of gliomas. Although the role of WWOX in the cytoskele...
Background
WW Domain Containing Oxidoreductase ( WWOX ) belongs to the unusual tumor suppressors, whose molecular function is not fully understood in bladder cancer, especially regarding interaction with Activator Protein 2 (AP-2) α/γ transcription factors. Thus, using lentiviral systems we created an in vitro model overexpressing or downregulating...
Introduction
The presence of common fragile sites is associated with no-accidental chromosomal instability which occurs prior to carcinogenesis. The WWOX gene spans the second most active fragile site: FRA16D. Chromosomal breakage at this site is more common in bladder cancer patients who are tobacco smokers which suggests the importance of WWOX ge...
Angiogenesis is the process of generating new capillaries from pre-existing blood vessels with a vital role in tumor growth and metastasis. MicroRNAs (miRNAs) are noncoding RNAs that exert post-transcriptional control of protein regulation. They participate in the development and progression of several cancers including bladder cancer (BLCA). In ca...
[IV-1] WWOX MAY LOSE ABILITY TO REGULATE PRO-TUMORAL AP-2Γ BUT STILL POSSESSES SYNERGISM WITH TUMOR SUPPRESSOR AP-2Α IN HIGH-GRADE BLADDER CANCER. Damian Kołat1, Żaneta Kałuzińska1, Elżbieta Płuciennik1 1 Department of Molecular Carcinogenesis, Medical University of Lodz, Poland Introduction: The WW Domain Containing Oxidoreductase (WWOX) is import...
Among all causes of death, cancer is the most prevalent and is only outpaced by cardiovascular diseases. Molecular theory of carcinogenesis states that apoptosis and proliferation are regulated by groups of tumor suppressors or oncogenes. Transcription factors are example of proteins comprising representatives of both cancer-related groups. Exempla...
The WW domain-containing oxidoreductase (WWOX) gene locus is positioned on chromosome 16 in a common fragile site FRA16D (16q23.1-q23.2) and it has a broad impact on cell function. The influence of the WWOX on many biological processes was found, regardless of the type of tissue. Of all cancers, little is known about the role of this gene in brain...
Czynnik transkrypcyjny AP-2γ wykazuje charakter onkogenny w raku pęcherza moczowego-wraz ze wzrostem jego ekspresji obserwuje się krótszy czas przeżycia pacjentów oraz niekorzystne parametry kliniczne. Jednym z supresorów nowotworowych istotnym w biologii raka pęcherza moczowego oraz oddziałującym z AP-2γ jest WWOX, którego spadek ekspresji skutkuj...
WWOX-AP-2α interaction affects biology of bladder cancer and demonstrates usefulness in its clinical features' discrimination. WWOX tumor suppressor gene is located in direct proximity to chromosomal locus 16q24 that is related to tumor progression of 20-45% bladder cancers. It encodes protein containing two WW domains, the first of which recognize...
Introduction:
The WW Domain Containing Oxidoreductase (WWOX) tumor suppressor gene is located in direct proximity to chromosomal locus 16q24 that is related to tumor progression of 20-45% bladder cancers. It encodes protein containing two WW (tryptophans) domains and short chain dehydrogenase domain. The first WW domain can recognize proline-rich m...
Introduction
The WW domain-containing oxidoreductase (WWOX) gene locus is positioned on chromosome 16 in a common fragile site FRA16D (16q23.1-q23.2) and is unclassifiable as its action is not restricted to direct control of the cell cycle or genome integrity, but exerts a more global impact on cell function. Despite the knowledge about the WWOX ge...
In vitro experimental model: CAL-29 bladder cancer cell line (grade 4) with different level of WWOX and high AP-2γ ü How performed: We used knock-in lentivirus and CRISPR/Cas9 system to overexpress and silence WWOX gene, respectively ü Assay types: adhesion to extracellular matrix proteins, colony formation, apoptosis, growth in 3D, activity of met...
https://network.febs.org/channels/2082-febs-2019-poster-introductions/posts/51113-febs-2019-congress-poster-video-introductions-thank-you-and-well-done
The WWOX gene is one of the unusual tumor suppressor genes, whose molecular function has not been fully understood in bladder cancer. The gene encodes a protein containing two Nterminal WW domain and the centrally located short chain dehydrogenase domain (SDR). The first WW domain interacts with AP2γ transcription factor (encoded by TFAP2C gene)...
Long non‑coding RNAs (lncRNAs) comprise a sizeable class of non‑coding RNAs with a length of over 200 base pairs. Little is known about their biological function, although over 20,000 lncRNAs have been annotated in the human genome. Through a diverse range of mechanisms, their primary function is in the regulation of the transcription of protein‑co...
WWOX tumor suppressor interacts with several transcription factors such as AP-2α and
AP-2γ. Based on literature data, WWOX protein represses oncogenic activity of AP-2γ in cytoplasm through inhibition of its transfer to nucleus and therefore impedes expression of target genes for this transcription factor. In terms of AP-2α, the molecular mechanis...
The regulation of AP2-α transcription factor via WWOX protein and its influence in bladder carcinogenesis.
Żaneta Kałuzińska1, Damian Kołat1, Magdalena Orzechowska2, Elżbieta Płuciennik2
1Faculty of Biomedical Sciences and Postgraduate Education, Medical University of Lodz, Poland
2Department of Molecular Carcinogenesis, Medical University of Lodz...
![Table 1 . Superclasses of transcription factors (based on [19] and...](profile/Damian-Kolat/publication/331457773/figure/tbl1/AS:731808001949696@1551488032339/Superclasses-of-transcription-factors-based-on-19-and-websites_Q320.jpg)
![Figure 1. Steps of cancerogenesis (based on [3])](profile/Damian-Kolat/publication/331457773/figure/fig1/AS:737072465862657@1552743178623/Steps-of-cancerogenesis-based-on-3_Q320.jpg)
The AP-2 transcription factor family is vital for the regulation of
gene expression during early development as well as carcinogenesis process. The review focuses on the
AP-2α and AP-2γ proteins
and their dualistic regulation of gene expression in the process
of carcinogenesis. Both AP-2α and AP-2γ influence a wide range
of physiological or pa...
Questions
Questions (2)
Dear Researchers,
On behalf of MDPI Cells Editorial Office and other Guest Editors, I sincerely encourage you to submit your prominent research to the wider scientific community. The Special Issue "Determination of AP-2 Transcription Factors Role in Carcinogenesis" belongs to the section "Cell Microenvironment" and submission deadline is 18 January 2023.
Guest Editors: Elżbieta Płuciennik, Żaneta Kałuzińska-Kołat, Damian Kołat
If you are interested in contributing, please let us know – ideally please provide tentative title and planned submission date. Please forward this message to any colleague that you think may be interested in contributing. The journal Cells (https://www.mdpi.com/journal/cells) has a rigorous peer-reviewing process for all manuscripts and provides the first decision to Authors in less than 16 days after submission. Acceptance to publication is undertaken in less than 3 days. The Article Processing Charge (APC, https://www.mdpi.com/about/apc) is 2200 CHF per accepted paper. Please note that for papers submitted after 31 December 2022, an APC of 2400 CHF applies.
In the case you need any assistance with regard to your submission, please never hesitate to contact the Editorial Office (cells@mdpi.comor bonnie.chen@mdpi.com) or Guest Editors (elzbieta.pluciennik@umed.lodz.pl; zaneta.kaluzinska@umed.lodz.pl; damian.kolat@umed.lodz.pl).
We are looking forward to receive your positive feedback!
Hi community,
I know the question might be misleading (this tool is free for registered users that need it for academic use) but the thing is, Oncomine Research Edition will be taken offline 17 January 2022.
I was trying to register and ultimately contacted ORE Technical Support - the message I received included the information that "the application is unable to register new users". Given the fact that these will be the last months of this platform, I was wondering if someone has an account that probably will not be using, or has data there that will not prevent sharing (e.g. via private message).
Thank you in advance.
Regards,
Damian
Projects
Projects (3)
Determination of molecular pathways that can be regulated by the WWOX protein in interaction with AP2α and AP2γ transcription factors in the bladder cancer cell lines RT-112 and CAL-29. To this end, tests were carried out regarding apoptosis and expression of selected genes of target transcription factors.
Determining collaboration of WWOX gene in the modulation of cellular pathways activated with AP-2α and AP-2γ transcription factors in bladder cancer
