Dalia Aboelhassan

• Professor of Biotechnology
• Professor at National Research Center (Giza)

CRISPR-Cas9 has emerged as a powerful tool in livestock breeding, enabling precise genetic modifications to address genetic diseases, enhance productivity, and develop disease-resistant animal breeds. A thorough analysis of previous research highlights the potential of CRISPR-Cas9 in overcoming genetic disorders by targeting specific mutations in g...

Background Ashwagandha plant enhances the body’s defense against toxicants through improving the cell-mediated immunity. Objective The present work was performed to assess the protective and therapeutic efficiencies of ashwagandha seed extract (ASE) against the harmful effects of amoxicillin (AM) treatment on biological and physical parameters in...

In recent years, strategic plans for poultry production have emphasized quantitative traits, particularly body weight and carcass traits (meat yield), in response to overpopulation challenges. Candidate genes such as adenylosuccinate lyase (ADSL), melanocortin-4-receptor (MC4R), and calpain 1 (CAPN1) have played vital roles in this context due to t...

Keywords Chicken breeds Pit-1 gene PCR-RFLP DNA sequencing Economic traits Chicken Pit-1 gene could be a candidate locus that affects important economic production traits. So, the present work was conducted to detect and analyze the different patterns (genotypes) in five reported polymorphisms (MR1-MR5) of such genes and investigate their effects o...

The present investigation was aimed to evaluate the protective and therapeutic effect of Fagonia critica extract (FCE) against Bisphenol A (BPA) induced genotoxicity and histopathology in rats. Genetic study included expression of P450 aromatase and Tph2 genes in the brain tissues in addition to expression of P450 aromatase and COX-1 genes in testi...

Background Genetic variants of the GDF9 gene were considered to be the potent gene markers for improving fecundity traits in Egyptian sheep and goats. Also, these favorable gene variants could be applied in the breeding program by gene-assisted selection (GAS), aiming towards the potential amelioration of reproduction and production in such small r...

Aflatoxin B1 (AFB1) was revealed to have potent mutagenicity and carcinogenicity. The consumption of nutritional products contaminated with AFB1 and inducing of genotoxicity and hepatocellular carcinoma have received widespread attention. The present study was aimed to investigate whether Moringa oleifera leaf extract (MOLE) can suppress AFB1-induc...

Aflatoxin B1 (AFB1) was revealed to have potent mutagenicity and carcinogenicity. The consumption of nutritional products contaminated with AFB1 and inducing of genotoxicity and hepatocellular carcinoma have received widespread attention. The present study was aimed to investigate whether Moringa oleifera leaf extract (MOLE) can suppress AFB1-induc...

Background and Objective: All living organisms could be exposed to Benzo[a]pyrene (BaP) that resulted mainly from burning of industrial carbon compounds, volcanic eruptions and forest fires causing genotoxic and carcinogenic effects. The current study aimed to examine the protective role of Echinacea purpurea (E purpurea) against BaP-induced genoto...

The present work was designed to study the genetic polymorphism in BMP15 gene at four sites, FecX B , FecX G , FecX H and FecX I and its association with improving twin production of Egyptian sheep and goats. This study was performed on 139 females including 113 mothers (83 of sheep and 30 of goats) at first , second, third and fourth parity and 26...

Genetic polymorphism of cGH gene using PCR-RFLP and identification of SNP between different genotypes detected in major Egyptian chicken breeds were studied. Amplification of intron 4 for locus GH2 and locus GH3 produced 563 bp fragment length. The amplified fragments (563 bp) were digested with MspI endonuclease. Locus GH2 showed three genotype fr...

In Nile tilapia (Oreochromis niloticus), pollution was detected from Lake Qarun, Wadi El-Rayan and Fish Farm at Fayoum Governorate, Egypt using RAPD-PCR and histopathological technique. The effective number of alleles was detected in Wadi El-Rayan population, while the lowest values were found in Lake Qarun population. Lake Qarun and Fish Farm show...

Genetic differentiation within and among three populations of Egyptian tilapia species (Oreochromis niloticus, Oreochromis aureus and Tilapia zillii) were examined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of D-loop mitochondrial DNA gene. A total of 16 phenotypes corresponding to 11 different haplotypes we...

A comparative transcription analysis of Ig κ-light chains (IGK) of the cattle breeds Holstein Friesian (HF), German Black Pied (GBP), German Simmental (GS) and Aubrac (A) revealed three alleles coding for two putative allotypic variants of IGKC. The amino acid residues p.Asp100Asn and p.Thr116Ala were located at the outer edge of the constant domai...

In the cattle breeds German Black Pied (GBP), German Simmental (GS), Holstein Friesian (HF), Aubrac (A) three transcribed allotypic variants in isotype IGLC2 and five allotypic variants in isotype IGLC3 were identified. Substitutions within the putative interface to CH1 at position 11 and 79 were noted. In IGLC2(b), K79E led to a charge conversion....