Dalanda Wanes

University of Veterinary Medicine Hannover | TiHo · Institute of Biochemistry

Niemann–Pick type C (NPC) disease is an autosomal recessive lysosomal storage disorder where 95% of the cases are caused by mutations in the Niemann–Pick C1 (NPC1) gene. Loss of function in NPC1 mutants trigger the accumulation of cholesterol in late endo-lysosomes and lysosomal dysfunction. The current study examined the potential of polyphenol-ri...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mainly targets the upper respiratory tract. It gains entry by interacting with the host cell receptor angiotensin-converting enzyme 2 (ACE2) via its heavily glycosylated spike glycoprotein. SARS-CoV-2 can also affect the gastrointestinal tract. Given the significant role of glycosylation...

Sucrase-isomaltase (SI) is the major disaccharidase of the small intestine, exhibiting a broad α-glucosidase activity profile. The importance of SI in gut health is typified by the development of sucrose and starch maldigestion in individuals carrying mutations in the SI gene, like in congenital sucrase-isomaltase deficiency (CSID). Common and rare...

Medicinal plants are known by pharmacological relevance and were used for long time to prevent/treat numerous gastrointestinal (GI) disorders. The current study focuses on the phytochemical/antioxidant characteristics of sage aqueous extract (SAE), as well as its pharmacological actions on altering motor function in the intestine and related disrup...

Niemann Pick type C (NPC) disease is an autosomal recessive lysosomal storage disorder that is caused by mutations in Niemann-Pick C1 (NPC1) or Niemann-Pick C2 (NPC2) genes ultimately leading to progressive neurological deterioration. 95% of NPC cases arise from mutations in the NPC1 gene. NPC1 protein mediates the intracellular cholesterol traffic...

The betacoronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the ongoing worldwide pandemic. The spike (S) glycoprotein of SARS-CoV-2 is a heavily glycosylated trimer that binds to host cell receptor angiotensin-converting enzyme 2 (ACE2), initiating viral entry. Evidence also shows that the S glycoprotein intera...

Patients with the rare autosomal recessive disorder congenital lactase deficiency (CLD) present with severe, potentially life-threatening symptoms shortly after birth. Several variants have been characterized within the gene for lactase-phlorizin hydrolase (LCT) that are associated with CLD. Here, we analyze at the biochemical and cellular levels L...

Cell lines are widely used as in vitro model systems and substitute for animal experiments. The frequently used Caco-2 cell line is considered to reflect characteristics of differentiated intestinal epithelium. However, the need to culture the cells with fetal calf serum (FCS) induces a high variability, risk of contamination and is ethically dispu...

Staphylococcus aureus is an important nosocomial and community-acquired facultative intracellular pathogen. Many studies have reported that S. aureus infections are associated with intestinal symptoms, but little is known about the molecular mechanisms implicated in S. aureus-induced alterations of intestinal functions. In this study, we investigat...

Rosa canina L. is a natural polyphenol-rich medicinal plant that exhibits antioxidant and anti-inflammatory activities. Recent in vivo studies have demonstrated that a methanol extract of Rosa canina L. (RCME) has reversed an inflammatory bowel disease (IBD)-like phenotype that has been triggered by dextran sulfate sodium (DSS) in mice. In the curr...

Rosa canina is a well-known medicinal plant used in folk remedy that alleviates various disorders, including inflammation, gastritis, and diarrhea. The objective of this investigation was to identify and quantify the phenolic components of R. canina methanolic extract (RCME) and to determine its protective action with dextran sulfate sodium (DSS)-g...

Background Chronic constipation is a gastrointestinal functional disorder which affects patient quality of life. Therefore, many studies were oriented to search herbal laxative agents. In this study, we investigated the effect of Globularia alypum L. leaves aqueous extract (GAAE) against loperamide (LOP)‐produced constipation. Methods Animals were...

A key morphological feature during the pathogenesis of inflammatory bowel disease (IBD) is the increased permeability of intestinal epithelial cells. Here, we address the implication of ER stress in protein and membrane trafficking in a dextran sulfate sodium (DSS)‐induced IBD‐like phenotype of absorptive intestinal Caco‐2 cells. We show that DSS t...

Congenital lactase deficiency (CLD) is a rare autosomal recessive genetic disorder, leading to a dysfunctional lactase‐phlorizin hydrolase (LPH). LPH is a ß‐galactosidase located in the intestinal brush border membrane and indispensable for lactose digestion. Impaired LPH‐function results in severe symptoms within a few days after birth with the on...

Congenital sucrase-isomaltase deficiency (CSID) is an autosomal recessive disorder of carbohydrate maldigestion and malabsorption caused by mutations in the sucrase-isomaltase (SI) gene. SI, together with maltase-glucoamylase (MGAM), belongs to the enzyme family of disaccharidases required for breakdown of -glycosidic linkages in the small intesti...

Thyme is a rich source of bioactive phytochemicals and it is frequently used in folk-medicine to treat gastroenteritis irritations. The current study was performed to examine the potential of Thymus vulgaris aqueous extract (TV-AE) to protect against delayed gastric emptying (DGE) and colonic constipation in rats. Stomach ulcer was caused by a sing...

Congenital lactase deficiency (CLD) is a severe autosomal recessive genetic disorder that affects the functional capacity of the intestinal protein lactase-phlorizin hydrolase (LPH). This disorder is diagnosed already during the first few days of the newborn’s life due to the inability to digest lactose, the main carbohydrate in mammalian milk. The...

Globularia alypum L. (GA) is a mediterranean plant of the Globulariaceae family. GA aqueous extract (GAAE) is widely used to treat various ailments, including constipation, peptic ulcer, diabetes, insomnia, hernia and intestinal pain. In this study we analyzed the chemical composition of GAAE by HPLC-PDA/ESI-MS and we examined its protective effect...

Ethnopharmacological relevance: Ficus carica fruit, a source of bioactive functional ingredients, have been traditionally long time used for its medicinal benefits as they improve the digestive system, treating constipation and used as a natural laxative. Aim of the study: The recent study was investigated the ameliorative effect of Ficus carica...

The aim of the present study is to evaluate the effect of chamomile (Matricaria recutita L.) decoction extract (CDE) on aspirin-induced small bowel injuries. The chemical composition of CDE was analysed by LC-HRESIMS. Experimental animals received CDE (100 mg kg⁻¹, p.o. P.C.) before induction of intestinal injuries by oral aspirin administration. C...

Introduction: Mallaw (Malva sylvestris L.) is a medicinal plant who is traditionally being used as an antiulcer, laxative and anti-hemorrhoid, besides of its culinary use as a food in Tunisian cuisine. The present study was carried out to evaluate the protective effect of Malva sylvestris aqueous extract (MSAE) on constipation- induced by loperami...

conference paper to 13th scientific journey of Tunisian Society of Immunology, 10-12 march 2016 Hammamet TUNISIA

Living organisms evolve at present in environments polluted by various types of pollutants. The increasing production of manufactured materials has led to the release of different kinds of particles in the aquatic environment. Bivalve organisms, especially mussels, concentrate the contaminants present in the medium. Increasing applications of titan...

Aquatic environments drain and collect the majority of the contaminants from commercial, industrial, and residential areas, representing a potential risk to the marine biota. Marine organisms particularly mussels concentrate contaminants, especially metals, depending on the concentrations present in the medium. Bivalves are considered the most pote...

This updated mini handbook (Version 3) could be used by student or researcher desiring to evaluate some immune parameters for two species of marine organisms Teleost fish (vertebrates) and mediterranean mussel (invertebrates), it contain new mentionned (plus those of the previous editions) practices, methdologies and detailed processes of some assa...