Dahai Gao

• PhD
• Professor (Associate) at Shanghai Ocean University

In plant, reactive oxygen species (ROS) play important regulatory roles in cell differentiation and development. The archeospores released from the blades of Pyropia yezoensis have important application value as secondary seedling sources in cultivation. However, whether ROS are involved in the production and early germination of archeospores of P....

Large-scale Ulva-caused green tides has posed various environmental and ecological problems as well as economic consequences. It is important to identify them accurately and quickly. The current universal markers based on ITS, rbcL, and 5S spacer sequences were defective to distinct closely related species in the genus of Ulva. In this study, by in...

In the present study, the whole chloroplast (cp) genome of B. marginata was characterized for the first time and genomic features were comparatively analyzed with six relative species in Ulvales. The cp genome of B. marginata was 170, 562 bp in length, exhibiting similar general structure but different in GC content from Ulva and Pseudendoclonium....

Reactive oxygen species (ROS) play important regulatory roles in plant growth and development, as well as in cell differentiation and stress responses. Respiratory burst oxidase homolog (RBOH) is the key enzyme in ROS production. So far, the Rboh family genes in Pyropia yezoensis have not been comprehensively characterized, and whether their functi...

Small heat-shock proteins (sHSPs/HSP20s) are closely related to algae and plant thermotolerance, but studies on HSP20 family in Neoporphyra haitanensis are scarce. In this study, a total of eight HSP20 members were identified from the genome of N. haitanensis. It was predicted that most of them encode acidic hydrophilic proteins, locating in variou...

As an important seedling source, monospores closely associate with yields in nori farming. However, the molecular mechanism underlying differences in monospore production for different strains remains unknown. Comparative transcriptome analysis was performed to examine gene expression differences between the spore abundant wild-type strain (WT) and...

Calmodulin (CaM) plays an important regulatory role in plant growth and development. In this study, on the basis of previous transcriptomic annotation a homolog of CaM gene (Pc-CaM7) was identified from the red alga Phycocalida chauhanii (previously known as Porphyra chauhanii). It encoded a 151 amino acids protein with 4 EF-hand regions, which was...

Nori is the cultivated seaweed with the highest market value in the world. Monospores from the thalli reseed the cultivating nets and provide useful secondary seedlings in nori farming, but the underlying molecular basis remains unclear. To explore crucial genes and pathways related to monospore-producing process in Phycocalida chauhanii (Pyropia c...

As an economically important marine seaweed, Pyropia yezoensis can be asexually reproduced via archeospores. However, the molecular mechanism of archeospore formation remains unclear. In this study, we characterized the transcriptomes of two strains of Py. yezoensis, which are strain W′ with abundant archeospores and strain W with scarce archeospor...

An edible green algal species Caulerpa lentillifera J. Agardh is reported from China for the first time. The species was collected from the southwest of Hainan Island and morphologically identified to be C. lentillifera based on the grape-like branches arising from cylindrical stolons. Phylogenetic analysis using tufA and rbcL DNA sequences also co...

Splicing of plant organellar group II introns is under accurate nuclear control that employs many nucleus‐encoded protein cofactors from various families. For mitochondrial introns, only a few splicing factors have been characterized because disruption of their functions often causes embryo lethality. Here, we report the function of Empty Pericarp8...

Caulerpa cupressoides (Vahl) C. Agardh is a widely distributed tropical green algae. The circular chloroplast genome was 130,895 bp in length, with a GC content of 34%. In total, 99 genes were identified and they were consisted of 63 coding genes, 30 tRNA genes, and 6 rRNA genes. This chloroplast genome did not show an obvious quadripartite structu...

The whole chloroplast genome (cp DNA) sequence of Caulerpa lentillifera J. Agardh has been characterized from Illumina pair-end sequencing. The circular cpDNA was 119,402 bp in length, containing 122 genes, which included 91 protein-coding genes, 28 tRNA genes, and 3 ribosomal RNA genes (four rRNA species). The overall AT content of C. lentillifera...

Dictyopteris divaricata is a kind of important brown algae with many biological activities. It has been receiving more and more attention, yet there are rarely studies done on its polysaccharides. In this study, the optimum extraction and biological activity of seaweed polysaccharides from Dictyopteris divaricata (DDSP) were investigated. Response...

Animals living at high altitudes have evolved distinct phenotypic and genotypic adaptations against stressful environments. We studied the adaptive patterns of altitudinal stresses on transcriptome turnover in subterranean plateau zokors (Myospalax baileyi) in the high-altitude Qinghai-Tibetan Plateau. Transcriptomes of zokors from three population...

The species-level taxonomy of the marine brown algal genus Sargassum is problematic. To resolve some of these systematic issues in the northern China seas, a molecular analysis of Sargassum species was carried out using ITS-2, cox 3, and rbc L DNA sequences. Phylogenetic analyses confirmed eight species, including: S. confusum , S. fusiforme , S. h...

This report describes the draft genome sequences of two strains, Pseudoalteromonas telluritireducens DSM 16098 and P. spiralis DSM 16099, which were isolated from hydrothermal vents of the Juan de Fuca Ridge. The reads generated by an Ion Torrent PGM were assembled into contigs with total sizes of 4.4 Mb and 4.1 Mb for DSM 16098 and DSM 16099, resp...

This report describes the draft genome sequence of Alcanivorax sp. strain KX64203, isolated from deep-sea sediment samples. The reads generated by an Ion Torrent PGM were assembled into contigs, with a total size of 4.76 Mb. The data will improve our understanding of the strain’s function in alkane degradation.

The neuroendocrine-immune (NEI) regulatory network is a complex system, which plays an indispensable role in the immunity of the host. In the present study, the bioinformatical analysis of the transcriptomic data from oyster Crassostrea gigas and further biological validation revealed that oyster TNF (CgTNF-1 CGI_10018786) could activate the transc...

Bacterial disease has caused high mortality of breeding molluscs from 2009 to 2011 in the Changhai area (Dalian, China). Vibrio spp. and Pseudomonas spp. have been detected as major pathogenic agents for aquatic animals in this area. In the present study, four virulence genes including vsm, toxR, aprX and carA were targeted to develop a real-time P...

A Yesso scallop pathogenic Vibrio splendidus strain JZ6 was found to be the highest virulence at 10 °C, while its pathogenicity was significantly reduced with the increase of temperature and completely incapacitated at 28 °C. In the present study, the comparative transcriptome analysis between JZ6 and another non-pathogenic V . splendidus strain TZ...

Background: Neural-endocrine-immune (NEI) system is a major modulation network among the nervous, endocrine and immune system and weights greatly in maintaining homeostasis of organisms during stress and infection. Some microRNAs are found interacting with NEI system (designated NeurimmiRs), addressing swift modulations on immune system. The oyste...

Gene duplication has been proposed to serve as the engine of evolutionary innovation. It is well recognized that eukaryotic genomes contain a large number of duplicated genes that evolve new functions or expression patterns. However, in mollusks, the evolutionary mechanisms underlying the divergence and the functional maintenance of duplicate genes...

Supplementary File 1. Full protein coding sequences of identified Pacific oyster protein kinase proteins genes (FASTA format). Supplementary File 2. Amino acid alignment of identified Pacific oyster protein kinase proteins sequences (FASTA format). Supplementary File 3. RPKM values for identified Pacific oyster protein kinase genes from five differ...

RNA editing, converting cytidines (C) to uridines (U) at specific sites in the transcripts of mitochondria and plastids, plays a critical role in organelle gene expression in land plants. Recently pentatricopeptide repeat (PPR) proteins were identified as site-specific recognition factors for RNA editing. In this study, we characterized an empty pe...

Tumor necrosis factor superfamily (TNFSF) members represent a group of cytokines participating in diverse immunological, pathological and developmental pathways. However, compared with deuterostomia and cnidaia, the composition and evolution of TNF homologous in protostomia are still not well understood. In the present study, a total of 81 TNF supe...

Thiamine pyrophosphate (TPP)-dependent enzymes are present in all domains of life, which share a cofactor with thiamine pyrophosphate. However, a comprehensive analysis of TPP-dependent enzymes from sequenced genomes has not been reported and little is known about the evolutionary dynamics of this gene family in plants. In this study, the evolution...

Micro ribonucleic acids (miRNAs) represent a class of small noncoding RNAs that play important roles in multiple biological processes by degrading targeted mRNAs or by repressing mRNA translation. In the case of algal lineages, especially dinoflagellates, knowledge regarding the miRNA system is still limited and its regulatory role remains unclear....

Detailed information of all the predicted miRNAs. Two sheets are in the EXCEL file containing the information of predicted miRNAs and the predicted targets, respectively. Sheet 1 lists all the information of the putative miRNAs including their length, accession number, mature position, hairpin position, orientation, hairpin sequence, and structures...

Statistical characteristics of Alexandrium tamarense ESTs.

Stem–loop structures for pre-miRNAs.

Pinus densata is a homoploid hybrid species, originating from P. tabuliformis×P. yunnanensis. The physiological fitness of this natural hybrid compared to its two parental species remains unknown. In this study, we investigated physiological responses of the three species by exposing artificially breed seedlings of each to drought stress lasting 28...

The sfr6-1 mutant of Arabidopsis thaliana was identified previously on the basis of its failure to undergo acclimation to freezing temperatures following exposure to low positive temperatures. This failure is attributed to a defect in the pathway leading to cold on-regulated (COR) gene expression via CBF (C-box binding factor) transcription factors...

Thellungiella salsuginea (Brassiaceae) is a typical halophyte which can tolerate extreme cold, drought, and salinity. In order to understand the adaptive evolution of this species in the arid habitats, it is important to know its genetic structure. In this study, 17 polymorphic microsatellite loci were isolated and characterized from an enrichment...