Dagmar Chudobova

Central European Institute of Technology | CEITEC

Nanoparticles of metals interacting with cellular components and biomacromolecules including DNA and RNA alter cellular processes. From the point of view of the antimicrobial activity, the metal nanoparticles at nanomolar concentrations exhibit excellent results on the inhibition of bacterial strains. The general mechanism of metal nanoparticles ac...

Background: Infections, mostly those associated with colonization of wound by different pathogenic microorganisms, are one of the most serious health complications during a medical treatment. Therefore, this study is focused on the isolation, characterization, and identification of microorganisms prevalent in superficial wounds of patients (n=50)...

Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen resistant to β-lactam antibiotics. Due to its resistance, it is difficult to manage the infections caused by this strain. We examined this issue in terms of observation of the growth properties and ability to form biofilms in sensitive S. aureus and MRSA after the applicatio...

The effects of heavy metals (Zn 2+ , Cu 2+ , and/or Cd 2+) on Escherichia coli expressing either prion (hPrP C) or metallothionein 3 (MT-3) brain proteins capable of binding these metals were investigated. The expression of hPrP C or MT-3 in E.coli was confirmed using western-blot and dot-blot methods. After analyzing growth curves, we found that b...

The application of ultraviolet (UV) radiation to inhibit bacterial growth is based on the principle that the exposure of DNA to UV radiation results in the formation of cytotoxic lesions, leading to inactivation of microorganisms. Herein, we present the impacts of UV radiation on bacterial cultures’ properties from the biological, biochemical and m...

Herein we describe a synthesis and testing of composites based on graphene oxide as a carrier in composites with metal or metalloid based nanoparticles (Cu, Zn, Mn, Ag, AgP, Se) as an antimicrobial agent for bacterial strains (Staphylococcus aureus (S. aureus), methicillin-resistant S. aureus (MRSA) and Escherichia coli (E. coli)). The composites w...

1-(1H-Benzimidazol-2-yl)-N-(1H-benzimidazol-2-ylmethyl)methanamine (abb) and 2-(1H-benzimidazol-2-ylmethylsulfanylmethyl)-1H-benzimidazole (tbb) have been prepared and characterized by elemental analysis. These bis(benzimidazoles) have been further used in combination with trithiocyanuric acid for the preparation of complexes. The crystal and molec...

In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase—GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli O...

In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase—GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli s...

Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen occurring not only in hospitals but also in foodstuff. Currently, discussions on the issue of the increasing resistance and timely and rapid diagnostic of resistance strains become more frequent and sought. Therefore, the aim of this study was to design an effective platform...

Kovove nanocastice interakcemi s buněcnými casticemi a biomakromulekulami vcetně DNA a RNA pozměňuji buněcne procesy. Pokud jde o antimikrobialni aktivitu, kovove nanocastice v nanomolarnich koncentracich inhibuji růst bakterialnich kmenů. Přestože obecný mechanismus ucinků kovových nanocastic dosud nebyl plně poznan, v soucasnosti je mezi nimi uzn...

The platinum group elements are significant compounds used in numerous fields of human life. Besides of often discussed toxic effect the platinum compounds show the therapeutic effects. Nowadays, platinum-based cytostatic are still the most frequently used drugs in oncology. Due to their proved medicinal purposes the behavior in the organism should...

In this study, we focused on the effect of heavy metal ions in resistant strains of gram-positive bacteria Staphylococcus aureus using biochemical methods and mass spectrometry. Five nitrate solutions of heavy metals (Ag+, Cu2+, Cd2+, Zn2+ and Pb2+) were used to create S. aureus resistant strains. Biochemical changes of resistant strains in compari...

This study was focused on the application of electrochemical methods for studying of bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after the application of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 μM). Bacterial strains Escherichia coli and Escherichia coli...

Remote-controlled robotic systems are being used for analysis of various types of analytes in hostile environment including those called extra-terrestrial. The aim of our study was to develop a remote-controlled robotic platform (ORPHEUS-HOPE) for bacterial detection. For the platform ORPHEUS-HOPE a 3D printed flow chip was designed and created wit...

The early detection of HIV-positive individuals before infecting healthy individuals is the basic principle to fight against AIDS. Although a lot of methods has been developed to detect HIV, electrochemistry is a promising tool thanks to its high sensitivity, low cost, ease of use and mostly no antibody-based detection. The aim of our study was to...

This study points to changes in the properties of liposomes and its functional qualities in task of release of encapsulated drug doxorubicin according to the content of cholesterol in the phospholipid bilayer. The influence of sodium dodecyl sulphate (SDS) addition to liposomal variants was also evaluated too. Three variants of liposomes differing...

Carbon nanomaterials, including fullerenes, exhibit not only unique structure and electronic properties but also a significant potential to serve as radical scavengers and/or anti-oxidants. Their conjugation with anticancer drugs such as doxorubicin may help to balance severe negative side effects of these cytostatics and also improve the delivery...

Interactions of silver phosphate nanoparticles (SPNPs) and selenium nanoparticles (SeNPs) with Staphylococcus aureus cultures have been studied at the cellular, molecular and protein levels. Significant antibacterial effects of both SPNPs and SeNPs on S. aureus were observed. At concentration of 300 μM, SPNPs caused 37.5% inhibition of bacterial gr...

There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant st...

The aim of the study was the preparation of a liposome complex with encapsulated lead ions, which were electrochemically detected. In particular, experiments were focused on the potential of using an electrochemical method for the determination of free and liposome-encapsulated lead and determination of the encapsulation efficiency preventing the l...

The physicochemical properties of liposomes are significantly affected by the composition of phospholipid bilayer; differences in composition allow the use of liposomes for analytical purposes and for therapeutic purposes. One of the most used components of phospholipid bilayer is cholesterol. Its concentration plays a significant role in the behav...

Bacterial infections caused by Gram-or Gram + bacteria can be a serious complication in the treatment of many diseases. These strains are a common producer of nosocomial infections, which can be acquired in hospitals. The aim of many studies is to prevent these infections using various technical and technological procedures. For comparison of the a...

Human papillomaviruses (HPV) are small circular, double-stranded DNA viruses infecting epithelial tissues. HPV types can be classified both as high-risk or low-risk. Of the more than 120 different identified types of HPV, the majority are involved in infections of the genital tract, cancer of the cervix, vulva, vagina and penis, and of non-anogenit...

Polymers are currently widely used to replace a variety of natural materials with respect to their favourable physical and chemical properties, and due to their economic advantage. One of the most important branches of application of polymers is the production of different products for medical use. In this case, it is necessary to face a significan...

We were focused on the studying oxidative stress in bacterial culture of Staphylococcus aureus induced by silver ions. The effect of the seven concentrations of AgNO3 (10, 25, 50, 75, 150, 225 and 300 μM) in bacterial culture was studied. Further, antioxidant activity of bacterial lysates of Staphylococcus aureus alone and incubated with different...