D. G. Deryabin

State Research Center of Dermatovenerology and Cosmetology

An analysis of the relationship between plasma cytokines and the effectiveness of treatment with TNFαinhibitors was performed in 81 patients with moderate-to-severe psoriasis. Treatment efficacy was assessed by PASI score, and patients were classified into a positive effect (PASI≥75) and no effect group (PASI≤50). A positive effect was reached in 1...

Based on data from 16 years of the N. gonorrhoeae antibiotic resistance monitoring program. gonorrhoeae in the Russian Federation were analyzed according to the minimum suppressive concentrations (MSC) of antimicrobial drugs against gonococcal strains obtained and characterized at the State Scientific and Research Center for Drug Control of the Min...

Quorum sensing inhibitors (QSIs) are an attractive alternative to antibiotic growth promoters in farmed animal nutrition. The goal of the study was the diet supplementation of Arbor Acres chickens with quercetin (QC), vanillin (VN), and umbelliferon (UF), which are plant-derived QSIs preliminarily showing cumulative bioactivity. Chick cecal microbi...

Background. The initial stages of androgenetic alopecia in men are characterized by a variety of clinical manifestation in the parietal or occipital scalp regions. However, the differences in the pathogenesis of hair loss patterns are not well understood, selective treatment has not been developed. Aims. Trichological characteristics of patients w...

The goal of this work was to determine the factors affecting the emergence of azithromycin-resistant Neisseria gonorrhoeae isolates in Russia, where azithromycin was never recommended for the treatment of gonococcal infections. Clinical N. gonorrhoeae isolates collected in 2018-2021 (428 isolates) were analyzed. No azithromycin-resistant isolates w...

Pemphigus vulgaris is a severe, socially significant autoimmune disease associated with autoantibodies to the desmoglein 3 antigen. The disease affects all age groups, beginning at 18 years of age; the mortality rate of pemphigus can reach as high as 50%, depending on a patients age and a number of other factors. There is no highly selective or per...

It is known that the inclusion of antibiotics in chicken feed leads to the development of resistance in bacteria. It is for this reason that the search for additives to alternative antibiotics becomes relevant. The ability of gamma-glucanolactone to inhibit various quorum systems (QS) LuxI/LuxR in bacteria has been demonstrated. Based on this, it c...

The aim of this work was to study the resistance to macrolides (azithromycin) in the modern Russian population of N. gonorrhoeae with the analysis of genetic resistance determinants. Azithromycin is not used to treat gonococcal infection in Russia. However, among 162 isolates collected in 2020–2021, 22 isolates (13.6%) were phenotypically resistant...

GOAL Of the study was molecular typing of current N. gonorrhoeae clinical isolates in Russia, aimed to identification of epidemiologically significant NG-MAST and MLST sequence types carrying a «mosaic» penA gene allele with reduced sensitivity to third-generation cephalosporins, which are recommended as first-line antimicrobials for gonococcal inf...

Many current gonococcal clinical isolates in Russia show atypical taxonomically significant biochemical activity, which leads to species misidentification. Molecular typing of such cultures according Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) and multilocus sequence typing (MLST) protocols assigned them to the G807 NG-MAST GENOGRO...

Implementation of the Russian version of Gonococcal Antimicrobial Surveillance Programme (RU-GASP) in XXI century is summarized. The chronology of evidence-based updating of national clinical guidelines for the gonococcal infection management is outlined. The reasons for penicillins, tetracyclines, and fluoroquinolones excluding from gonococcal inf...

Neisseria gonorrhoeae plasmids can mediate high-level antimicrobial resistance. The emergence of clinical isolates producing plasmid β-lactamases that can hydrolyze cephalosporins, the mainstay treatment for gonorrhea, may be a serious threat. In this work, N. gonorrhoeae strains producing plasmid-mediated broad-and extended-spectrum β-lactamases (...

N. gonorrhoeae strains producing plasmid-mediated broad- and extended-spectrum beta-lactamases (ESBLs) have been obtained in vitro and studied for their viability and susceptibility to beta-lactam antibiotics. The artificial pblaTEM-1 and pblaTEM-20 plasmids were constructed by site-directed mutagenesis from a pblaTEM-135 plasmid of the Toronto/Rio...

Comparative whole-genome analysis was performed for Neisseria gonorrhoeae isolates belonging to the Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) types predominant worldwide — 225, 1407, 2400, 2992, and 4186 — and to genogroup 807, the most common genogroup in the Russian Federation. Here, for the first time, the complete genomes of...

A multiplex assay based on a low-density hydrogel microarray was developed to identify genomic substitutions in N. gonorrhoeae that determine resistance to the currently recommended treatment agents ceftriaxone and azithromycin and the previously used drugs penicillin, tetracycline, and ciprofloxacin. The microarray identifies 74 drug resistance de...

We analyzed the sequence of the gene encoding 16S ribosomal RNA in 2 samples containing a mixture of human DNA and Mycobacterium leprae DNA obtained from two patients from the Russian Federation. We found that our sequence matched the sequence of the reference strain deposited at the National Center for Biotechnology Information (NCBI, USA). The an...

Background. Androgenic alopecia (AGA) is the most common form of pathological hair loss with multiple micronutrient disorders involvedin its occurrence and development. Aimto evaluatethe effectiveness of personalized treatment of micronutrient deficiencies in patients with early stages of AGA and conservative therapy using a vasodilator drug minoxi...

A multiplex assay based on a low-density hydrogel microarray was developed to identify genomic substitutions in N. gonorrhoeae that determine resistance to the currently recommended treatment agents ceftriaxone and azithromycin and the previously used drugs penicillin, tetracycline, and ciprofloxacin. The microarray identifies 74 drug resistance de...

A method for the analysis of the epitope specificity of auto-reactive antibodies to desmoglein 3 (Dsg3) using competitive ELISA has been developed. It is based on a two-stage solid-phase ELISA with initial "depletion" of auto-reactive antibodies against the studied epitope and subsequent quantitative assessment of antibodies against full-length ext...

Androgenetic alopecia (AGA) is the most common variant of male pattern baldness in which occurrence and development of multiple genetic, hormonal, and metabolic factors are involved. We aimed to estimate plasma element content (Mg, Ca, Zn, Cu, Se, Fe), vitamin status (B12, D, E, and folic acid) in patients with AGA using direct colorimetric tests o...

Background Decreased susceptibility of Neisseria gonorrhoeae to extended-spectrum cephalosporins is a major concern. Elucidation of the phenotypic and genetic characteristics of such isolates is a priority task. Methods We developed a method for predicting the N. gonorrhoeae ceftriaxone susceptibility level (MICcro) by identifying genetic determin...

Using the recombinant second fragment of the extracellular domain (EC2) of human desmoglein type 3 (Dsg3) as an affinity ligand, an immunosorbent was obtained that selectively binds autoreactive antibodies to this domain from the immune sera of patients with pemphigus. The EC2 protein was obtained in the form of a fusion protein with the Fc-fragmen...

One of the target drugs for plaque psoriasis treatment is apremilast, which is a selective phosphodiesterase 4 (PDE4) inhibitor. In this study, 34 moderate-to-severe and severe plaque psoriasis patients from Russia were treated with apremilast for 26 weeks. This allowed us to observe the effectiveness of splitting patient cohorts based on clinical...

Coumarins are class of natural aromatic compounds based on benzopyrones (2H-1-benzopyran-2-ones). They are identified as secondary metabolites in about 150 different plant species. The ability of coumarins to inhibit cell-to-cell communication in bacterial communities (quorum sensing; QS) has been previously described. Coumarin and its derivatives...

Metal nanoparticles (NPs) with antibacterial properties represent a promising alternative approach to antibiotics , whose overuse has led to the appearance of drug-resistant bacteria. This article addresses particularly copper (Cu) nanoparticles since Cu is a structural constituent of many enzymes in living microorganisms. In addition, Cu has a bet...

Background.Psoriasis is a widespread skin disorder characterized by cytokines as the main modulators of skin inflammation. A promising psoriasis therapy approach is targeted on cytokine networks by inhibiting the phosphodiesterase 4 (PDE-4). The efficacy and safety of the PDE-4 inhibitor apremilast in psoriasis confirmed in multicenter controlled s...

This work aimed to study penA gene polymorphisms in clinical isolates of Neisseria gonorrhoeae collected in Russia in 2018-2019 and the contribution of the penA allele type to susceptibility to β-lactam antibiotics. A total of 182 isolates were analyzed. penA allele types were determined by sequencing, and the minimum inhibitory concentrations (MIC...

Leprosy was modeled in an experiment on BALB/c, BALB/cNude, CBA, and C57BL/6ТNF-/- mice using three Mycobacterium leprae strains obtained from patients with a diagnosis of A30 according to ICD-10 from different regions of the Russian Federation. Proliferation of M. leprae of the used strains showed a temporal-quantitative dependence on the used mou...

The most common symptoms of COVID‐19 are fever, malaise, headache, muscle pain, dry cough and pneumonia, which showed the disease as an upper respiratory tract viral infection. The first observation about COVID‐19 patients who develop cutaneous manifestations was done by S. Recalcati (1). In numerous French (2), Belgian (3) Spanish (4) and Italian...

Androgenic alopecia (AGA) is the most common type of progressive hair loss in man. The search for reliable predictors of the conservative treatment's effectiveness is an urgent problem today. Forty-eight patients with AGA, stages I-IV by the Norwood-Hamilton scale, were treated for 4 months with 5% topical minoxidil joints with corrections for trac...

Based on the data about angiotensin-converting enzyme 2 (ACE2) as the main receptor for SARS-CoV-2, hematogenous spread of the virus is hypothesized, followed by killing of ACE-2-expressing cells in the microvascular channels. Damage to the blood vessels (primarily arterioles) leads to the development of microtromboses and results in the exit of th...

Surveillance of multidrug-resistant infections is a priority task for contemporary epidemiology. The aim of this study was to genotype modern clinical isolates of N. gonorrhoeae using the NG-MAST technique (Neisseria gonorrhoeae multi-antigen sequence typing) and to compare the phylogeny of the gonococcal pathogens coming from Russia, European Unio...

Based on arp, tprII and tp0548 genes variability molecular typing of Treponema pallidum is a worldwide method for syphilis epidemiology analysis, including the ability to monitor antimicrobial-resistant variants of the causative agents of the disease.The aim of the study is to characterize the molecular subtypes of T. pallidum isolated from syphili...

Aim. To develop a method for coamplification of single-copy genes and repetitive elements of the Mycobacterium leprae genome in the analysis of clinical material from leprosy patients with an assessment of the clinical significance of the study results.Materials and methods. Skin scarification and biopsy samples from patient R. with a diagnosis of...

Objectives: The goal of this work was to assess the genetic diversity ofNeisseria gonorrhoeae isolates in Russia and Europe and to compare the distribution of the N. gonorrhoeae multi antigen sequencing types (NG-MAST) of Russian isolates with that of isolates from European countries. Methods: NG-MAST typing was performed for 804N. gonorrhoeae i...

The purpose of this study was to assess the biological activity and antiquorum activity of extracts of Lamiaceae plants. Material and methods. Extracts of 4 medicinal plants of the Lamiáceae were used as test substances. Bioluminescent analysis were used on the model Escherichia coli MG1655 and antiquorum activity of medicinal plant extracts - on t...

A total of 399 Neisseria gonorrhoeae clinical isolates collected in different regions of the Russian Federation in 2015-2017 were analyzed for tetracycline susceptibility and genetic markers of resistance. Drug susceptibility testing was performed by serial dilution method in agar and minimum inhibitory concentrations (MICs) were determined accordi...

Numerous gram-negative phytopathogenic and zoopathogenic bacteria utilise acylated homoserine lactone (AHL) in communication systems, referred to as quorum sensing (QS), for induction of virulence factors and biofilm development. This phenomenon positions AHL-mediated QS as an attractive target for anti-infective therapy. This review focused on the...

Aim . Analysis of the arp gene internal fragment nucleotide sequences variability in modern russian T. pallidum subsp. pallidum strains. Materials and methods. 57 T. pallidum isolates obtained from specialized dermatovenerologic clinics of the Central (Kaluga), the North Caucasus (Stavropol) and the Siberian (Tyva) regions in 2016 — 2017 were used...

Background: Among pathological hair loss conditions in men the androgenic alopecia (L64 according to ICD-10) has been the most common diagnosed. However, the reasons of the occurrence and development of the disease remain incompletely clarified, that determines the difficulties of personalized therapy. Aims: To analyze both genetic and non-genetic...

The goal of this work was to study the phenotypic susceptibility and resistance determinants of N. gonorrhoeae isolates to beta-lactam antimicrobials (benzylpenicillin and ceftriaxone). A total of 522 clinical isolates collected in Russia in 2015–2017 were analysed for susceptibility using the agar dilution method. DNA loci involved in antimicrobia...

Three water-soluble C60-fullerene derivatives functionalized by anionic adducts of various chemical compositions and structures were prepared in highly specific reactions of chlorofullerene C60Cl6. Hybrid metal-organic complexes formed and aggregated in several instances in aqueous solution in the presence of stoichiometric (1:1 by moles) amounts o...

The Russian Gonococcal Antimicrobial Surveillance Programme (RU-GASP) was established in 2004 and operated continuously during the years 2005-2016. The aims of this study were to summarize the RU-GASP results over this 12-year period and evaluate the trends in Neisseria gonorrhoeae antimicrobial resistance in Russia. In total, 5,038 verified N. gon...

132 clinical isolates of N. gonorrhoeae collected in Arkhangelsk region during 2014–2016 were analyzed in accordance with the NG-MAST protocol (Neisseria gonorrhoeae Multi Antigen Sequence Typing). A diversity of the analyzed strains was represented by 53 NG-MAST sequence-types; 30 of them were new registered due to earlier unknown either porB and...

The study was aimed at effective inhibition of quorum sensing (QS) in the model bacterium Chromobacterium violaceum ATCC 31532 by combinations of compounds with different anti-QS activity mechanisms. These mechanisms were: (1) suppression of autoinducer (C6-AHL) biosynthesis by subinhibitory concentrations of an aminoglycoside antibiotic amikacin;...

The male-specific phenotype termed androgenetic alopecia or male pattern hair loss (MPHL ) often affects quality of life. The development of this pattern is mainly caused by a wide spectrum of genomic peculiarities involving metabolism, follicle development, cell signaling, autophagy, and epigenetic regulation. In this study the prediction model pr...

Certain level of new registered cases of leprosy in a number of endemic countries in the world, as well as growing rate of transboundary migratory flows, raise the issue of effective diagnosis of this disease in countries with sporadic incidence of leprosy, including the Russian Federation. The purpose of the study was to develop a highly sensitive...

An immunochip for multiple parallel detection of specific serum IgG in serological screening for syphilis is based on the use of an extended array of Treponema pallidum recombinant proteins and includes traditionally used immunodominant antigens (Tp15, Tp17, Tp47, and TmpA) and new synthetic proteins (Tp0277, Tp0319, Tp0453, Tp0684, Tp0965, and Tp1...

The whole-genome sequencing data of three N. gonorrhoeae strains isolated in the Russian Federation in 2015 are presented. According to the NG-MAST protocol, these strains are related to the globally spread ST 1407 genogroup. The analysis of their resistomes showed the absence of ermA/B/C/F genes and the presence of wild-type alleles of rpsE, rrs,...

Progressive growth in the degree of antimicrobial resistance of N. gonorrhoeae creates a prospect for gonorrhea to move towards the category of potentially incurable infections. Genetic determinants of N. gonorrhoeae resistance to antimicrobial drugs used for the therapy of gonorrhea in Russia during the last decade have been analyzed in clinical i...

The whole-genome sequencing data of three N. gonorrhoeae strains isolated in the Russian Federation in 2015 are presented. According to the NG-MAST protocol, these strains are related to the globally spread ST 1407 genogroup. The analysis of their resistomes showed the absence of ermA/B/C/F genes and the presence of wild-type alleles of rpsE, rrs,...

The 132 gonococcal strains collected in Arkhangelsk (Russia) in 2014—2016 were typed according Neisseria gonorrhoeae multi antigen sequence typing (NG-MAST) protocol. The high population diversity was described: 53 NG-MAST types were revealed where 30 had new porB or tbpB allele or allele’s combination and were firstly described NG-MAST types. Thre...

The objective of this study was to estimate the tetracycline resistance level in the modern population of Neisseria gonorrhoeae in the Russian Federation, where this drug was removed from the treatment regimen for gonococcal infections in 2003. A total of 401 isolates collected between 2015 and 2017 were analyzed for genetic markers (chromosomal po...

Effect of aminoglycosides (kanamycin, gentamycin, and amikacin) and tetracyclines (tetracycline and doxycycline) on the CviI/CviR two-component quorum sensing mediated with C6-AHL autoinducer in Chromobacterium violaceum ATCC 31532 was studied. All antibiotics showed growth-inhibitory activity against the test strain and violated its density-depend...

Molecules of plant origin are characterized by various forms of action on the bacterial cells. This is caused by the constant interaction and adaptation of o