Cun Zhang

Cun Zhang
China Academy of Chinese Medical Sciences | CACMS · Institute of Chinese Material Medica

PhD

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78
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Publications

Publications (78)
Article
Full-text available
The intrinsic chemical components and sensory characteristics of Gardeniae fructus Praeparatus (GFP) directly reflect its quality and subsequently, affect its clinical curative effect. However, there is little research on the correlation between the appearance traits and chemical compositions of GFP during heat processing. In this study, the major...
Article
Full-text available
The processing method for Chinese traditional herbal medicine is “Pao Zhi” in Chinese. This study examined the efficacy of the Pao Zhi on the preparations of Gardeniae Fructus (GF) on a mitochondrial respiratory function in rats. To determine the efficacy of Pao Zhi, we investigated the effects of GF heat processing on mitochondrial respiratory fun...
Article
Full-text available
Four new cytochalasans, phychaetoglobins A–D (1–4), together with twelve known cytochalasans (5–16), were isolated from a mangrove-associated fungus Chaetomium globosum kz-19. The new structures were elucidated on the basis of extensive 1D and 2D NMR, HR ESIMS spectroscopic analyses, and electronic circular dichroism (ECD) calculations. The absolut...
Article
Fast development of combination of nanotechnology with traditional Chinese medicine (TCM) broadens the field of application of TCM. Besides, it increases the research ideas and contributes to TCM modernization. As expected, TCM will be developed into the nanodrug delivery system by nanotechnology with careful design, which will enhance the medicina...
Article
Crocins, as a kind of water-soluble carotenoid pigment, are a series of ester compounds formed from crocetin and gentibiose or glucose, and mainly distributed among Crocus sativus L. (CSL), Gardenia jasminoides Ellis. (GJE). Crocins exhibit a wide range of pharmacological effects on neurodegeneration, cardiovascular disease, cerebrovascular disease...
Article
The chromatic values of the broken-fried and single-fried Gardeniae Fructus Praeparatus(GFP) were measured by the color analyzer to analyze the color variation rule, and the contents of 10 main components were determined by ultra-high performance liquid chromatography(UPLC). The multivariate statistical analysis, Pearson correlation analysis, and d...
Article
As an important treasure for the research and development of new Chinese medicines, the classical prescriptions have a long medical history in China and are the summary of the clinical experience of the medical experts in the past dynasties. To support the research and development of classical prescriptions,relevant encouraging policies were issued...
Preprint
Full-text available
Background: Codonopsis Radix (CR) as the tonic Chinese herbis is good at benefiting Qi and promoting saliva, while rice-processed CR (RCR) can alleviate the original dryness and more specialize in invigorating the spleen and stopping diarrhea. Despite CR and RCR are long-standing medicines commonly used in clinic, information on their differences i...
Preprint
Full-text available
Background Zhi-Zi-Da-Huang decoction (ZZDHD) is a famous Traditional Chinese Medicine decoction due to its therapeutic effects on clinical hepatobiliary disorders. ZZDHD is composed of Gardeniae Fructus, Rhei Radix et Rhizoma, Fructus Aurantii Immaturus, and Sojae Semen Praeparatum. With the development of current technology, dispensing granules ha...
Article
The chromaticity space parameters of the samples during the processing of Gardeniae Fructus Praeparatus(Jiaozhizi in Chinese herbal name, JZZ) were measured by the visual analyzer to analyze the color variation rule during the processing of JZZ, and the content changes of total reducing sugar, total amino acid and 5-hydroxymethylfurfural(5-HMF) rel...
Article
The aim of this paper was to investigate the hemostatic effect and mechanism of carbonized Scutellariae Radix on uterine bleeding in the rats caused by early pregnancy termination. Eight unpregnant female rats were selected as normal group. Forty female rats conceived on the same day received mifepristone(11. 4 mg·kg-1) and misoprostol(125 μg·kg-1)...
Article
Traditional Chinese Medicines (TCMs) have been used for more than 3,000 years. However, most TCMs are used based on previous experience, and their exact mechanisms are still unclear. Proteomics, as a major component of systems biology, is an appropriate tool that could bring breakthroughs in the study of pharmacological mechanisms of TCMs. Research...
Article
Full-text available
Gardeniae Fructus (GF) and carbonized GF (GFC) have been shown to exert a gastrointestinal protective effect and are frequently used in clinical practice for the treatment of hemorrhage and brown stool. In this study, we employed a combination of pharmacological methods and metabolomics in a rat model of ethanol-induced acute stomach ulcer to inves...
Article
In this experiment,the gradation analysis method was used to evaluate the quality of different pieces of Gardeniae Fructus through the extraction rate difference and the difference analysis of the main components in the extract. In this experiment cold-dip and hot-dip methods were used to compare the yield of Gardeniae Fructus extract and the conte...
Article
Full-text available
Gardeniae Fructus (GF), prepared GF (GFP), and carbonized GF (GFC) are widely used in China for the treatment of fever. However, the involved antipyretic mechanism has not been fully elucidated. In this study, rectal temperature and pyrogenic cytokines were used to evaluate the antipyretic effect of raw and processed GF in rats with dry-yeast-induc...
Article
Commercially available japonica rice and indica rice with different trade names were collected,and then based on the method of rice stir-frying,their many indexes were evaluated,for example the physical and chemical properties such as appearance color,grain type,broken kernel ratio,length-width ratio,1 000-grain weight,specific heat capacity,moistu...
Article
A study was performed to compare the pharmacokinetics of major bioactive analytes in rhubarb plants to explore the pharmacological differences between raw pieces (RP) and steamed pieces (SP) of rhubarb. A rapid and efficient ultra-performance liquid chromatographic coupled with mass/mass spectrometry (UPLC-MS/MS) method was established to determine...
Article
Background Scutellariae Radix (Huangqin) is commonly processed into 3 products for different clinical applications. However, a simple analytical method for quality control has rarely been reported to quickly estimate the degree of processing Huangqin or distinguish differently processed products or unqualified Huangqin products. Objective To study...
Article
With the publishment of the application guideline for the key project of "Research on Modernization of Traditional Chinese Medicine" by the Ministry of Science and Technology in 2018, the problem of "intelligentization" in the production of decoction pieces of Chinese crude drugs has attracted more and more attention from the industry. Some suggest...
Article
By means of various chromatographic methods such as Sephadex LH-20,ODS,and semi-preparative HPLC,ten compounds were isolated from Streptomyces sp. A1693 and their structures were elucidated on the basis of spectroscopic data and physico-chemical methods. The compounds comprised 5 butenolides,2 diketopiperazines,and 3 antimycin antibiotics. The stru...
Article
Full-text available
Twelve new compounds including four isoflavonolignans (1a/1b and 2a/2b), two neolignans (3a/3b), and six isoflavonoids (5–10), together with seven known compounds (4 and 11–16) were isolated from the stems of Pisonia umbellifera. The structures were elucidated on the basis of comprehensive spectroscopic analyses and ECD calculation methods. Compoun...
Article
A reliable and rapid high-performance liquid chromatography coupled with diode array detector method (HPLC–DAD) was established and validated to determine eight gingerol simultaneously in the rhizomes of Zingiber offcinale Rosc. The separation of eight compounds (4-hydroxy-3-methoxy-benzenebutanol,3,5-dihydroxy-1-(4-hydroxy-3-methoxyphenyl) decane,...
Article
The qualitative analysis method of ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) was established for the chemical constituents in Sanhuang tablets. Waters ACQUITY BEH C₁₈ (2.1 mm×100 mm, 1.7 μm) column was used with 0.1% formic acid solution (A)-0.1% formic acid acetonitrile (B) as the...
Article
Full-text available
Elevated adipogenesis of bone marrow stromal cells (BMSCs) is closely associated with non-traumatic osteonecrosis of femoral head (ONFH). Our previous studies have shown that Huogu (HG) formula was effective both in clinic experience and experimental ONFH. How HG impacts the differentiation of BMSCs and what is the underlying molecular mechanism re...
Article
Our recent studies have shown that Huogu (HG) formula was effective both in clinic experience and in experimental osteonecrosis of the femoral head (ONFH). Given that defective of bone marrow stromal cells (MSCs) contribute to the development of osteonecrosis and MSCs show enormous potential in the treatment of ONFH, especially to aging people. How...
Article
Gardeniae fructus is one of the most frequently used herbs in traditional Chinese medicine. In the present study, a process for the enrichment of six iridoid glycosides from Gardeniae fructus was developed using medium-pressure liquid chromatography combined with macroporous resin and reversed-phase chromatography. The purities of different fractio...
Article
Gardeniae Fructus contains volatile ingredients, however, the species and proportions in different processed products of Gardeniae Fructus are different. In this experiment, volatile ingredients were separated by steam distillation with content of 1.2, 1.0, 0.9, 0.7 µL · g(-1) in Gardeniae Fructus, fried Gardeniae Fructus, stir-baked Gardeniae Fruc...
Article
Processed Chinese medicine is the core of traditional Chinese medicine (TCM) industry chain,which directly affects the clinical efficacy and. safety of Chinese patent medicine and clinical formula Decoction pieces. Studied the variation of effective substance in vivo Chinese medicine processing before and after processed, clarifying the effective s...
Article
As increasing numbers of people worldwide are using traditional Chinese medicines, their characterization has become increasingly critical. This study describes the development of a facile and reproducible high-performance liquid chromatography-based method to obtain the fingerprint of the traditional Chinese medicine Yuhuanglian. First, similarity...
Article
Rheum palmatum L. and Coptis chinensis Franch., are two representative cold-natured traditional Chinese medicine with heat-clearing effect, and were widely used to treat fever associated diseases in China for a long history. To elucidate the mechanism of the antipyretic effect of Rheum palmatum L. and Coptis chinensis Franch. from the perspective o...
Article
A facile and reproducible HPLC method was established for quality evaluation of fructus of Ligustrum lucidum Ait pieces, Both typical chromatograms analysis and determination analysis for main active ingredient were employed to evaluate the quality of fructus of Ligustrum lucidum Ait pieces. It is successfully applied to distinguish fructus of Ligu...
Article
Ethnopharmacological relevance and aim of the study: Wen Luo Yin (WLY) is a traditional Chinese formula, which has the traditional use of scattering cold pathogen, draining dampness, freeing the flow of network vessels and relieving pains. It is extensively used in the treatment of rheumatoid arthritis (RA) patients for more than 2000 years, but i...
Article
Ethnopharmacological relevance: Saposhnikovia divaricata (SD), called "Fangfeng" in China, is commonly used in clinical compound prescription for treatment of rheumatoid arthritis (RA), but its actions on RA have not been clarified. The present study aims to determine the anti-inflammatory activity of SD chromone extract (SCE), the major bioactive...
Article
To compare the contents of the main chemical compositions in Gardenia jasminoids before and after being roasted with ginger juice. Four diterpenoid pigments constituents (C-1, C-2, C-3, crocetin) were determined simultaneously by UPLC on an Agilent Poroshell 120 EC-C18 column at 35 degrees C with the methanol-0.5% formic acid anhydrous in gradient...
Article
For the purpose of stabilizing and enhancing the clinical effect, the author suggested that it should develop the key technology of integrated innovation research such as origin producing area processing technology, medicinal parts and energy-saving rapid drying technology, process control technology, quality evaluation key technology, packaging an...
Article
To compare the components and contents of volatile oil in Gardenia jasminoids, Zingiber officinale, G. jasminoids roasted with ginger juice and to provide scientific basis for intrinsic material of G. jasminoids roasted with ginger juice. The volatile oil in the three herbal medicines was extracted by steam distillation, the components were analyze...
Article
AimTo study the constituents of Rheum palmatum.Methods Chromatographic separations of silica gel and polyamide were used. All the structures were elucidated on the basis of chemical and spectroscopic analysis.ResultsNine compounds were isolated and their structure were identified as 2, 5-dimethyl-6, 8-dihydroxy-chromone (1), 1-methyl-2, 8-dihydroxy...
Article
Two new anthraquinone glycosides, named 1-methyl-8-hydroxyl-9,10-anthraquinone-3-O-β-D-(6'-O-cinnamoyl)glucopyranoside (1) and rhein-8-O-β-D-[6'-O-(3''-methoxyl malonyl)]glucopyranoside (2), have been isolated from the roots of Rheum palmatum, together with seven known compounds, rhein-8-O-β-D-glucopyranoside (3), physcion-8-O-β-D-glucopyranoside (...
Article
To establish the HPLC fingerprint method and compare the changes of chemical compositions of the processed products from Sinapis alba. The procedure of HPLC analysis was performed on a agilent TC-C18 (2) column at 35 degrees C with the acetonitrile -0.1% phosphoric acid in gradient elution as the mobile phase. The detection wavelength was set at 25...
Article
Full-text available
To establish a HPLC method for the determination of gallic acid and catechin in Rheum palametum and to study the changes of gallic acid and catechin content in R. palametum during processing. The contents of gallic acid and catechin were determined simultaneously by HPLC on the Zorbax Eclipse XDB-C18 (4.6 mm x 250 mm, 5 microm) column at 30 degrees...
Article
To compare the contents of two another anthraquinone-O-glucosides in five different processed products from Rheum palmatum. Aloe-emodin-3-CH2-O-beta-D-glucopyranoside and emodin-8-O-beta-D-glucopyranoside were determined simultaneously by HPLC on an Agilent TC-C18 (2) column at 35 degrees C with the tetrahydrofuran-1% glacial acetic acid (25:75). T...
Article
A new compound, 9,10-dihydrophenanthrinic acid, 9,10-dione-3,4-methylenedioxy-8-methoxy (1) was isolated from the roots of Peucedanum praeruptorum Dunn on the basis of spectral evidences.
Article
To study the chemical constituents of the roasted seeds of Cassia obtusifolia, and to illuminate the change of its effective components before and after being roasted. The compounds were isolated and repeatedly purified by macroporous resin, silica gel column chromatography. Their structures were elucidated by physical and chemical properties and N...
Article
To compare of the contents of two stilbene glycoside in five processed products of Rheum palamatum. The contents of trans-3, 5, 4'-trihydroxystilbene-4'-O-beta-D-glucopyranoside (a) and trans-3, 5, 4'-trihydroxystilbene-4'-Obeta-D-(6"-O-galloyl)-glucopyranoside (b) were determined by HPLC analysis at 35 degrees C with methanol-1% acetic acid as mob...
Article
OBJECTIVE: To compare the contents of coumarin in the different processed products from Peucedanum praeruptorum Dunn. METHODS: (±)- Praeruptorin A, (±)-Praeruptorin B and (+)-Praeruptorin E were determined simultaneously by HPLC. The separation was performed on a Agilent TC-C18(2) column at 35°C with MeOH-H2O(3:1) as the mobile phrase. The detectio...
Article
To compare the contents of two anthraquinone-O-glucosides in five different processed products from Rheum palamatum. The content of aleo-emodin-8-O-beta-D-glucoside, rhein-8-O-beta-D-glucoside were determined simultaneously by HPLC on a agilent TC-C18 (2) column at 35 degrees C with the mobile phase of acetonitrile-1% glacial acetic acid (20: 80)....
Article
A new trimeric linear-type furanocoumarin, rapultririn A (1), was isolated from the underground part of Heracleum rapula. The structure of this new compound was elucidated by NMR, MS spectroscopic methods.
Article
Two new glycosides, nor-rubrofusarin-6-O-β-d-(6′-O-acetyl)glucopyranoside (1) and 1-desmethylaurantio-obtusin-2-O-β-d-glucopyranoside (2) were isolated from the seeds of Cassia obtusifolia and their structures were established by chemical and spectral evidences.
Article
To observe the different effects of water soluble extracts from Cassia obtusifolia and processed C. obtusifolia on anti-oxidation and vasodilatation, and to explore the mechanism of eye-clearing effect. Mice were randomly divided into three groups: normal control group, C. obtusifolia group and processed C. obtusifolia group. Ten male and 10 female...
Article
To compare the contents of five anthraquinone components in five different processed products from Rheum palamatum. The contents of aleo-emodin, rhein, emodin, chrysophanol and physcion were determined simultaneously by HPLC on plogilent TC-C18 (2) column at 35 degrees C with the methanol-0.1% phosphoric acid (85: 15). The detection wavelength was...
Article
To compare the contents of iridoid among the fruit, seed, and pericarp from Gardenia jasminoides. The contents of geniposide and genipin gentiobioside were determined simultaneously by HPLC. A Kromasil C18 column at 35 degrees C was used with the acetonitrile-0.3% formic acid anhydrous (12: 88) as the mobile phases. The detection wavelength was set...
Article
To identificate and compare constituents in the HPLC fingerprint of five Chinese herbal pieces from Radix et Rhizoma Rhei. HPLC analysis was carried out with methyl alcohol 1% glacial acetic acid as gradient elution, changes in five Chinese herbal pieces and medicinal material under 280 nm and 430 nm were compared. HPLC fingerprints of the no-parch...
Article
To establish methods of RP-HPLC respectively for content-determination of two types of constituents in the Cassia obtusifolia respectively, and tp determine the constituents between the raw and roasted seeds of C. obtusifolia in order to distinguish the difference of those seeds. HPLC systems consisted of Alltima C18 (4.6 mm x 150 mm, 5 microm), co...
Article
To compare the contents of diterpenoid pigments among the fruit, seed, and pericarp from Gardenia jasminoides. The separation of Crocin 1, Crocin 2, Crocin 3 were carried out simultaneously on a Kromasil C18 column at 35 degrees C with the methanol-acetonitrile-0.3% formic acid anhydrous in gradient elution as the mobile phrases. The detection wave...
Article
To observe the effect of the main constituents and its combination from Chuanxiong Fangfeng Baizhi prescription on isolated rabbit basilar artery. The isolated rabbit basilar artery rings were treated with 5 mmol x L(-1) KCl and 5-HT 2.4 x 10(-8) mol x L(-1), and by measuring the tension of basilar artery rings, the effect of the main constituents...
Article
Objective: To study the chemical constituents in roots of Peucedanum praeruptorum. Method: The constituents were isolated by column chromatography on silica gel and ODS, and identified by spectroscopic methods. Result: Seven compounds, alpha-D-glucopyranose-1-hexadecanoate (1), D-mannitol monohexadecanoate (2), adenosine (3), butyric acid (4),...
Article
To establish HPLC fingerprint for the identification of Cassia obtusifolia and compare chemical constituents of the raw and roasted seeds of C. obtusifolia. Chromatographic fingerprint of C. obtusifolia was investigated by RP-HPLC and the gradient elution mode was applied to chromatographic separation. Data were analyzed by fingerprint similarity e...
Article
To investigate the chemical constituents of the roasted seeds of Cassia obtusifolia to illuminate the change of its effective components before and after roasted. Compounds were separated by silica gel chromatography, and their structures were evaluated by spectral analysis and chemical evidence. Seven compounds were isolated from the ethanol extra...
Article
To compare the contents of diterpenoid pigments in the different processed products of Gardenia jasminoides. The separation of Crocin 1, Crocin 2, Crocin 3, Crocetin were determined simultaneously by HPLC on a kromasil C18 column at 35 degrees C with the m methanol-acetonitrile-0.3% formic acid anhydrous in gradient elution as the mobile phrase. Th...
Article
To compare the contents of chromones in the different isolation and purification procedures from Saposhnikovia divaricata. The separation of six chromones was determined simultaneously by HPLC on a Kromasil C18 column at 35 degrees C with the methanol-0.1% phosphoric acid in gradient elution as the mobile phrase. The detection wavelength was set at...
Article
To compare the contents of iridoid in the different processed products from Gardenia jasminoides. Contents of geniposide and genipin gentiobioside in products were determined simultaneously by HPLC. A kromasil C18 column at 35 degrees C was used with the acetonitrile-0.3% formic acid anhydrous (12:88) as the mobile phrase. The detection wavelength...
Article
To research the expectorant components in volatile oil from the root and rhizome of Aster tataricus. GC-MS was applied to isolate and identify the compounds. In addition, TLC was used to isolate compound, and its structure was elucidated on the basis of spectral data analysis. At the same time, its expectorant effect was observed by method of the e...
Article
Objective: To study the chemical constituents in Gardenia jasminoides. Method: The constituents were isolated by column chromatography on silica gel and ODS, and identified by NMR, MS spectroscopic methods. Result: Nine compounds, imperatorin (1), isoimperatorin (2), crocetin (3), 5-hydroxy-7, 3', 4', 5'-tetrainethoxyflavone (4), 2-methyl-3, 5...
Article
Platycodon grandiflorum, as the traditional Chinese medicine, has been employed for dispersing the lung and resolving phlegm soothing the throat, and promoting pus discharge in ancient days. Triterpenoids are its main effective constituents which have dominant activities on antitussive, expectorant, antitumor, antioxidant and immune enhancement. Th...
Article
To isolate and identify the chemical constituents from the roots of Peucedantu praeruptorum. The constituents were isolated by column chromatography on silica gel and ODS, and identified by NMR, MS spectroscopic methods. Eight compounds, (-) sclerodin (1), palmitic acid (2), (+)-praeruptorin E (3), bergapten (4), imperatorin (5), 2,6-dimethyl quino...
Article
To study the chemical constituents in stem rind of Wikstroemia indica. The constituents were isolated by column chromatographies with silica gel and ODS, and identified by NMR, MS spectra. Six compounds, daphnoretin-7-O-beta-D-glucoside (1), quercitin (2), physcion (3), kaempferol 3-rutinoside (4), D-primev-ersyl genkwanine (5), and anabellamide (6...
Article
Objective: To study the chemical constituents in roots of Heracleum rapula. Method: The constituents were isolated by column chromatography on silica gel and ODS, and identified by spectroscopic methods. Result: Eight compounds, xanthotoxol (I), 8-geranyloxypsoralen (II), (+)-marmesin (III), beta-sitosterol (IV), stigmasterol (V), oleanolic ac...
Article
Objective: To study the chemical constituents in roots of Heracleum rapula. Method: The constituents were isolated by column chromatography on silica gel and ODS, and identified by spectroscopic methods. Result: Nine compounds, osthol ( I ), bergapten (II), xanthotoxin (III), isopimpinellin (IV), imperatorin (V), isoimperatorin (VI), cnidilin...
Article
Objective: To study the chemical constituents form stem bark of Wikstroemia indica. Method: The constituents were isolated by column chromatography on silica gel and ODS, and identified by spectroscopic methods. Result: Seven compounds, daphnoretin (I), tricin (II), daucosterol (III), beta-sitosterol (IV), umbelliferone (V), genkwanin (VI), an...
Article
To compare the pharmacological effect of the main coumarin constituents of Peucedanum praeruptorum from Jiangxi and He'nan. The constituents were isolated by column chromatography on silica gel and ODS and identified by NMR and MS spectroscopic methods. The antitussive, expectorant and anti-inflammatory effects of main coumarin constituents of P. p...
Article
To study the chemical constituents in roots of Peucedanum praeruptorum. The constituents were isolated by column chromatography on silica gel and ODS, and identified by MS spectroscopic methods including NMR and. Seven compounds, acetylatractylodinol (I), tanshinone II A (II), tanshinone I (III), cis-3', 4'-diisovalerylkhellactone(IV), cis-3',4'-di...
Article
Objective: To establish a quality standard of Peucedanum praeruptorum for Chinese Pharmacopoeia (2005). Method: The presence of (+/-)-praeruptorin A were identified and assayed by TLC and HPLC, respectively. Result: Linearity of marker was obtained over the range of 0.17696-0.88480 microg (r=0.9999). The average recovery rate was 99.72% (RSD=0...
Article
To work out a qualitay standard of Radix Angelicae dahuricae. TLC and HPLC were adopted. Four coumerin compounds were identified by TLC, and the contents of two compounds were determined by HPLC. Imperatorin, isoimperatorin, byakangelicin and oxypeucedaninhydrate can be found simultaneously in Radix Angelicae dahuricae. The contents of imperatorin...