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Introduction
I am currently studying the molecular mechanisms that drive neural crest cell development in the chicken, Gallus gallus. Eventually, I hope to identify the role of specific EMT-related cancer genes in neural crest cell EMT during embryonic development.
Current institution
Additional affiliations
January 2010 - present
Education
August 2003 - April 2009
September 1997 - January 2001
Publications
Publications (38)
Vertebrate development is regulated by several complex well-characterized morphogen signaling pathways, transcription factors, and structural proteins, but less is known about the enzymatic pathways that regulate early development. We have identified that factors from the inflammation-mediating cyclooxygenase (COX) signaling pathway are expressed a...
Our lab has identified that transcripts and proteins of the cyclooxygenase (COX-1 and COX-2) isoenzymes are expressed during the early stages of vertebrate embryonic development, and that global COX-1/2 inhibition disrupts neural crest (NC) cell maturation in Ambystoma mexicanum (axolotl) embryos, with intriguing implications for tissue regeneratio...
Vertebrate development is regulated by several complex well-characterized morphogen signaling pathways, transcription factors, and structural proteins, but less is known about the enzymatic pathways that regulate early development. We have identified that factors from the inflammation-mediating cyclooxygenase (COX) signaling pathway are expressed a...
The choice of fixation method significantly impacts tissue morphology and protein visualization after immunohistochemistry (IHC). In this study, we compared the effects of paraformaldehyde (PFA) and trichloroacetic acid (TCA) fixation prior to IHC on chicken embryos. Our findings underscore the importance of validating fixation methods for accurate...
Non-steroidal anti-inflammatory drugs (NSAIDs) are a class of analgesics that inhibit the activity of cyclooxygenase isoenzymes, which drive tissue inflammation pathways. Caution should be exercised when taking these drugs during pregnancy as they increase the risk of developmental defects. Due to the high rates of NSAID use by individuals, possibi...
Neural crest cells (NCCs) are a dynamic, multipotent, vertebrate-specific population of embryonic stem cells. These ectodermally-derived cells contribute to diverse tissue types in developing embryos including craniofacial bone and cartilage, the peripheral and enteric nervous systems and pigment cells, among a host of other cell types. Due to thei...
Ambystoma mexicanum (axolotl) embryos and juveniles have been used as model organisms for developmental and regenerative research for many years. This neotenic aquatic species maintains the unique capability to regenerate most, if not all, of its tissues well into adulthood. With large externally developing embryos, axolotls were one of the origina...
Neural crest (NC) cells are a dynamic population of embryonic stem cells that create various adult tissues in vertebrate species including craniofacial bone and cartilage and the peripheral and enteric nervous systems. NC development is thought to be a conserved and complex process that is controlled by a tightly regulated gene regulatory network (...
Neural crest (NC) cells are a dynamic population of embryonic stem cells that create various adult tissues in vertebrate species including craniofacial bone and cartilage and the peripheral and enteric nervous systems. NC development is a conserved and complex process that is controlled by a tightly regulated gene regulatory network (GRN) of morpho...
Neural crest (NC) cells are multipotent embryonic cells that form melanocytes, craniofacial bone and cartilage, and the peripheral nervous system in vertebrates. NC cells express many cadherin proteins, which control their specification, epithelial to mesenchymal transition (EMT), migration, and mesenchymal to epithelial transition. Abnormal NC dev...
Neural crest (NC) cells are multipotent embryonic cells that form melanocytes, craniofacial bone and cartilage, and the peripheral nervous system in vertebrates. NC cells express many cadherin proteins, which control their specification, epithelial to mesenchymal transition (EMT), migration, and mesenchymal to epithelial transition. Abnormal NC dev...
Neural crest cells are a transient stem-like cell population that forms in the dorsal neural tube of vertebrate embryos and then migrates to various locations to differentiate into diverse derivatives such as craniofacial bone, cartilage, and the enteric and peripheral nervous systems. The current dogma of neural crest cell development suggests tha...
The cover image, by Crystal D. Rogers and Shuyi Nie, is based on the Advanced Review Specifying neural crest cells: From chromatin to morphogens and factors in between, DOI: 10.1002/wdev.322. The cover image, by Crystal D. Rogers and Shuyi Nie, is based on the Advanced Review Specifying neural crest cells: From chromatin to morphogens and factors i...
This article contains data related to the research article entitled, "A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices" (Rogers et. al., 2018) [1]. The data presented here include (1) proximity ligation assays using antibodies that recognize type I cadherins (N-cadherin and E-cadherin) co-incu...
The neural crest, an embryonic stem cell population, initially resides within the dorsal neural tube but subsequently undergoes an epithelial-to-mesenchymal transition (EMT) to commence migration. Although neural crest and cancer EMTs are morphologically similar, little is known regarding conservation of their underlying molecular mechanisms. We re...
Elk3/Net/Sap2 (here referred to as Elk3) is an Ets ternary complex transcriptional repressor known for its involvement in angiogenesis during embryonic development. Although Elk3 is expressed in various tissues, additional roles for the protein outside of vasculature development have yet to be reported. Here, we characterize the early spatiotempora...
The neural crest is a transient population of multipotent and migratory cells unique to vertebrate embryos. Initially derived from the borders of the neural plate, these cells undergo an epithelial to mesenchymal transition to leave the central nervous system, migrate extensively in the periphery, and differentiate into numerous diverse derivatives...
The neural crest is a transient population of multipotent and migratory cells unique to vertebrate embryos. Initially derived from the borders of the neural plate, these cells undergo an epithelial to mesenchymal transition to leave the central nervous system, migrate extensively in the periphery, and differentiate into numerous diverse derivatives...
Fig. S1. FGF8a does not induce Xbra expression and transiently inhibits vent2 expression. (A) WISH for xbra using embryos that were uninjected (UI) or injected with mRNA coding for FGF8a or Nog+ FGF8a with lacZ (cyan) as a tracer and collected at stage 8 and every hour after until stage 10. Embryos were cultured at room temperature. There is no ect...
Fig. S2. BMP inhibition and FGF signaling induce the expression of early neural genes. (A-B) WISH for zicr1 and sox2 of embryos injected with mRNA coding for tBR, FGF8a or tBR + FGF8a and lacZ mRNA (cyan) and collected at stage 8 (t = 6 hpf) and each subsequent hour after until stage 10.5 (t = 10 hpf) when cultured at room temperature. (C) RT-PCR o...
Fig. S4. FGF8a induces n-tub positive neurons in the same tissue layer as epi-k positive epidermis. Bisections of stage 17 whole embryos show that Nog expands sox3 expression in the deep layer (marked by asterisk) and represses epidermal gene expression n-tub expression. FGF8a overexpression can expand n-tub expression in both the deep and superfic...
Fig. S3. CaBMPR is sufficient to repress the expression of early neural genes in gastrulae. (A-F) WISH of embryos stages 10.5 (A-E) or 12 (F) for genes as indicated next to each panel. Embryos were either uninjected (UI) or injected with constitutively active BMP receptor Alk3 (CaBMPR) and lacZ mRNA. Arrowhead points to site of injection. Pictures...
The molecular mechanism that initiates the formation of the vertebrate central nervous system has long been debated. Studies in Xenopus and mouse demonstrate that inhibition of BMP signaling is sufficient to induce neural tissue in explants or ES cells respectively, whereas studies in chick argue that instructive FGF signaling is also required for...
The neural ectoderm of vertebrates forms when the bone morphogenetic protein (BMP) signaling pathway is suppressed. Herein, we review the molecules that directly antagonize extracellular BMP and the signaling pathways that further contribute to reduce BMP activity in the neural ectoderm. Downstream of neural induction, a large number of "neural fat...
The SRY-related, HMG box SoxB1 transcription factors are highly homologous, evolutionarily conserved proteins that are expressed in neuroepithelial cells throughout neural development. SoxB1 genes are down-regulated as cells exit the cell-cycle to differentiate and are considered functionally redundant in maintaining neural precursor populations. H...
The formation of the nervous system is initiated when ectodermal cells adopt the neural fate. Studies in Xenopus demonstrate that inhibition of BMP results in the formation of neural tissue. However, the molecular mechanism driving the expression of early neural genes in response to this inhibition is unknown. Moreover, controversy remains regardin...
