Cristóbal Leonardo Domínguez Borbor

Escuela Superior Politécnica del Litoral (ESPOL) · Centro Nacional de Acuicultura e Investigaciones Marinas (CENAIM)

Some yeast strains have been proposed as probiotics to improve the health of cultured fish. Cobia is a tropical benthopelagic fish species with potential for marine aquaculture; however, one of the main limitations to its large-scale production is the high mortality of fish larvae. In this study, we evaluated the probiotic potential of autochthonou...

Pathogenic vibrios of farmed shrimp have acquired resistance to antibiotics commonly used in aquaculture, leading to a constant search for new, effective products to control them. In vitro methods currently used to evaluate possible solutions are expensive, tedious, and time-consuming. In contrast, the methyl thiazole tetra�zolium (MTT) reduction...

The use of probiotics, prebiotics and dietary fiber has become a common practice in shrimp aquaculture as alternatives to antibiotic treatment. However, not much is known about the metabolic mechanisms underlying the effects of probiotics and immunostimulant used in shrimp aquaculture. In this study, a gas chromatography−mass spectrometry (GC−MS) b...

The vibrios carrying the pirA and pirB toxin genes cause acute hepatopancreatic necrosis disease (AHPND) in cultivated shrimp, significantly affecting the yield. The use of probiotic strains is a promising strategy to prevent vibriosis in shrimp cultures. The microbial consortium formulated with three strains, Vibrio diabolicus (Ili strain), Vibrio...

Cationic peptides bio-inspired by natural toxins have been recognized as an efficient strategy for the treatment of different health problems. Due to the specific interaction with substrates from biological membranes, snake venom phospholipases (PLA2s) represent valuable scaffolds for the research and development of short peptides targeting parasit...

Background Acute hepatopancreatic necrosis disease (AHPND) is an important shrimp bacterial disease caused by some Vibrio species. The severity of the impact of this disease on aquaculture worldwide has made it necessary to develop alternatives to prophylactic antibiotics use, such as the application of probiotics. To assess the potential to use pr...

Outbreaks of white spot syndrome virus (WSSV) have caused serious damage to penaeid shrimp aquaculture worldwide. Despite great efforts to characterize the virus, the conditions that lead to infection and the infection mechanisms, there is still a lack of understanding regarding these complex virus-host interactions, which is needed to develop cons...

The emergence of new virulent Vibrio strains resistant to common antibiotics has caused significant economic losses to shrimp farming worldwide. It is mandatory to adopt new strategies to control shrimp farming related vibriosis. Essential oils (EOs) have several biological properties among of which the quorum sensing (QS) inhibitory activity is ap...

El cultivo de camarón es una de las principales actividades acuícola a nivel mundial, y para Ecuador representa el primer producto de exportación no petrolero. A pesar de su crecimiento expansivo, la industria camaronera es frecuentemente amenazada por brotes de enfermedades. Recientemente, varios vibrios han sido asociados a la enfermedad de necro...

White spot syndrome virus (WSSV) is one of the most virulent pathogens of cultured penaeid shrimp. Several control strategies are used commonly to mitigate the economic losses caused by the pathogen, such as application of antiviral products at farm level. One of the most practical method for the screening of potential anti-WSSV products is through...

In the search for probiotic bacteria from marine environments to control pathogenic Vibrio of cultivable marine species, this study focused on members of the genus Pseudovibrio. Based on its symbiotic features and antibacterial capacity, the genus Pseudovibrio could be used as probiotic to control vibriosis in shrimp culture. Fortythree isolates of...

Bacterial diseases cause high mortality in Penaeus (Litopenaeus) vannamei postlarvae. Therefore, appropriate application of efficient therapeutic products is of vital importance for disease control. This study evaluated through in vitro analyses the antimicrobial effectiveness of commercial therapeutic products used for P. vannamei bacterial diseas...

Antibiotics have been used massively for aquatic organism production. However, their use should be limited given the problems of increased antibiotic resistance, and new alternatives should be attempted, such as the use of plants extracts with antibacterial properties. The effect of the essential oil of oregano (Origanum vulgare) compared with thre...

El metaboloma es el conjunto de compuestos orgánicos de bajo peso molecular (metabolitos) pro- ducidos por sistemas biológicos. El antagonismo microbiano es una importante fuerza evolutiva, por lo que el análisis de su metaboloma es una herramienta útil para la búsqueda de nuevas molé- culas con actividad biológica. El objetivo de este trabajo fue...

The marine biodiversity program of CENAIM-ESPOL focuses on sessile invertebrates from the “‘El Pelado’ marine reserve”. The present study aims at understanding the different hierarchical levels of biodiversity found at the “El Pelado” and to guide biodiscovery initiatives. Generated biodiversity data were systematized in a database (Darwin Core sta...

The first chemical study of the marine sponge Callyspongia cf. californica widely distributed along the coasts of the Tropical Eastern Pacific led to the identification of a new family of amphiphilic derivatives called callyspongidic acids. The four isolated metabolites 1-4 feature a hydrophilic diacid end opposed to both an aromatic moiety and a l...

Despite a large occurrence, especially over the Pacific Ocean, the chemical diversity of marine invertebrates belonging to the order Zoantharia is largely underexplored. For the two species of the genus Antipathozoanthus no chemical study has been reported so far. The first chemical investigation of Antipathozoanthus hickmani collected at the Marin...

To mitigate the economic losses provoked by disease outbreaks, shrimp producers employ therapeutic additives. However, important issues such as the toxicity of these products on shrimp are not always considered. In vivo toxicity assays require a lot of time and large economic and physical resources. Here, we describe an in vitro procedure to evalua...

Las esponjas marinas constituyen una fuente rica de metabolitos bioactivos con propiedades antibacterianas, antitumorales, antifúngicas, antivirales y antinflamatorias. Sin embargo, no todas estas moléculas son producidas por las esponjas, estudios recientes indican que muchos de estos metabolitos son productos de los microorganismos asociados, ent...

El uso de antibióticos en la acuicultura del Ecuador es difícil de controlar y radicar por que los productores buscan formas rentables y efectivas, en los vegetales siempre se han encontrado fuentes de alternativas a estos temas, basado en un uso empírico y atoctono de la zona se ha trasladado la adición de extractos vegetales a los cultivos de cam...

Debido a las variaciones ambientales los sistemas de cultivo constituyen ambientes estresantes que favorecen la aparición y propagación de enfermedades infecciosas. Las prácticas de manejo para controlarlas han involucrado el uso de antibióticos y probióticos, muchas veces de forma empírica. Nosotros proponemos hacer una revisión profunda de esas p...