Cristina M Rocha

Cristina M Rocha
University of Minho · Centre of Biological Engineering

PhD in Chemical and Biological Engineering

About

68
Publications
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1,352
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Publications

Publications (68)
Article
Hydrolysates from high-protein coproducts are rich in bioactive peptides (small amino acid chains with properties beyond their nutritional value). When included in aquafeeds, they may promote fish robustness while increasing animal blood value and minimizing waste, contributing to a circular economy. This work assessed how innovatively produced blo...
Article
The bioavailability of natural compounds should be assessed through different perspectives. Studying the behaviour of the extracts after digestion is often overlooked but is crucial for success in the development of active food ingredients. Thus, the bioaccessibility of S. nigra (flower and berry) extracts after in vitro gastrointestinal digestion...
Article
Full-text available
This study examined the influence of ohmic heating (OH), compared to the conventional heating (Conven) and Control (solvent) methods, on the extraction of olive mill leaves. The main extraction parameters were: (i) solvent ratio (aqueous ethanol; 40%, 60%, and 80%, v/v), and (ii) extraction temperature; 45 °C, 55 °C, and 75 °C (for OH and Conven),...
Article
Full-text available
The red seaweed Gelidium sesquipedale is recognized for its high-quality agar, a valued hydrocolloid with gelling capacity, an industrially desirable property strongly dependent on its structure and composition. Despite its importance, there is a lack of information regarding the effects of processing conditions, including alternative extraction te...
Article
Full-text available
Green extraction techniques (GreETs) emerged in the last decade as greener and sustainable alternatives to classical sample preparation procedures aiming to improve the selectivity and sensitivity of analytical methods, simultaneously reducing the deleterious side effects of classical extraction techniques (CETs) for both the operator and the envir...
Article
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Recently, there are two main movements gaining public attention: a “green revolution” focused on the use of sustainable processes and the increasing concerns with health-promoting diets and the recognition of the gut microbiota and its effects on the treatment and prevention of several diseases. Microalgae and macroalgae arise as an interesting alt...
Article
Full-text available
Olive pomace (OP) is the main residue that results from olive oil production. OP is rich in bioactive compounds, including polyphenols, so its use in the treatments of diseases related to oxidative stress, such as cancer, could be considered. The present work aimed to study the biological properties of different OP extracts, obtained by ohmic heati...
Chapter
Pulsed electric fields (PEF) is attracting great attention as nonthermal extraction technology on downstream processing of emergent marine bioresources such as micro- and macroalgae. These uni- and multicellular algae enclose a diverse and interesting biochemical composition and are recognized as important sources of valuable bioactive compounds an...
Chapter
In the last decade, there was a great effort to promote lignocellulosic biomass (LCB) as a raw material for obtaining new added value compounds. Agricultural and industrial processing activities generate a large volume of by-products, most of which are still little explored, representing a significant risk to the environment. LCB is an abundant and...
Article
Full-text available
Eggplants contain a multitude of biocompounds with nutritional and/or biological activities. The objective of this work was to study the nutritional, chemical and bioactive value of red eggplant from Rotonda, Italy. Ohmic heating (OH) was compared to conventional heating, as different solvents were used (water, ethanol 30, 50, and 90% and methanol)...
Article
Electric field-based technologies for extraction processes have been gaining importance due to sustainability concerns. This work aims to assess the potential of ohmic heating as an efficient and feasible tool for the extraction of different biocompounds from Gracilaria vermiculophylla and its effect on the extracts' composition. Different ratios o...
Article
Full-text available
The effects of different light spectra on agar yield, composition and gel properties, pigment content and antioxidant activity of Gracilaria gracilis cultivated in a land-based IMTA-system were investigated. Total phenol content, antiradical activity and lipid peroxidation inhibitory capacity in the residue obtained from G. gracilis agar extraction...
Article
Microalgae are a promising and sustainable ingredient for functional food development, due to their rich nutritional and health-promoting effects. Moreover, microalgae generally contain large amounts of structural biopolymers such as proteins and polysaccharides, which might display interesting technological features and rheological properties. Thi...
Article
Full-text available
The main goal of this study was to chemically characterize an aqueous S. nigra flower extract and validate it as a bioactive agent. The elderflower aqueous extraction was performed at different temperatures (50, 70 and 90 C). The extract obtained at 90 ◦C exhibited the highest phenolic content and antiradical activity. Therefore, this extract was a...
Article
Tagatose is a rare sugar with increasing commercial interest as sweetener. Biotechnological production of d-tagatose by enzymatic isomerization of d-galactose provides an alternative to chemical processes. In the last years, l-arabinose isomerases (L-AIs) from different origins have been studied to increase the effectiveness of tagatose production....
Article
Agro-food industries generate a huge amount of fruit and vegetable wastes. These residues are composed by structural parts (leaves, peels, pulps, seeds, roots and stems) after the extraction of their juices, and they are rich in bioactive compounds such polyphenols, protein, carbohydates, fibre, lignin, lipids and minerals. Therefore, these wastes...
Article
A sequential multi-stage procedure was applied on the extraction of biocompounds from Spirulina platensis. The process consisted in three steps: 1) aqueous extraction, using conventional thermal extraction (CE), ohmic heating (OH, 7 V/cm), enzymatic treatment (EAE, 0.8 mgLysozyme/mL), or both OH and EAE combined; 2) ethanolic extraction; 3) CHCl3/M...
Article
Animal-based proteins are the most consumed worldwide given their well-balanced nutritional composition. However, the growing demand for animal proteins will not be sustainable due to their low conversion efficiency and high environmental footprint. Specific consumers’ dietary restrictions and modern trends emphasize the importance of finding alter...
Chapter
Full-text available
The significant increase in the world population age, 47 years in 1950 to 73 years in 2020, resulted in an increase in aging related diseases as well as in degenerative diseases. In consequence, researchers have been focusing in the development of new therapies, with a particular emphasis on the use of compounds with antioxidant properties, nam...
Article
Full-text available
Seaweeds are a renewable natural source of valuable macro and micronutrients that have attracted the attention of the scientists in the last years. Their medicinal properties were already recognized in the ancient traditional Chinese medicine, but only recently there has been a considerable increase in the study of these organisms in attempts to de...
Article
Full-text available
Polyphenolic extracts from pine bark have reported different biological actions and promising beneficial effects on human health. However, its susceptibility to environmental stresses (temperature, storage, etc.) and physiological human conditions prequires the development of efficient protection mechanisms to allow effective delivering of function...
Article
Generic cosmetic creams (oil-in-water emulsions) were prepared using dry Bacterial Cellulose and Carboxymethyl Cellulose (BC:CMC) to study the possibility of partially or completely replacing surfactants, while ensuring a long-term stability and the required organoleptic characteristics. BC:CMC was benchmarked against two hydrocolloidal Avicel prod...
Article
Fibers from passion fruit stalks were first recovered with a pulping and bleaching process. Two mechanical treatments were further applied to the fibers, homogenization (with and without ultrasound), and blender application. The effect of those treatments on fibers was evaluated. The chemical composition of the different stages of fibers undergoing...
Article
Seaweeds are promising feedstocks; nevertheless, the lack of systematic approaches to recover different high-value fractions in a clean and sustainable mode hampers their exploitation. Due to this necessity, an innovative environmentally friendly strategy was proposed in this article for the development of a sugar platform from Gelidium sesquipedal...
Article
The aim of this work was to study the potential application of edible films obtained by complex coacervation promoted by the electrostatic interactions between positively charged chitosan (CH) 3% (w/w) and negatively charged whey protein isolate (WPI) 3% (w/w). Preliminary assays of turbidimetry were made in order to find the optimal CH-to-WPI mass...
Article
Full-text available
The agricultural and processing activities of olive crops generate a substantial amount of food by-products, particularly olive leaves, which are mostly underexploited, representing a significant threat to the environment. Olive leaves are endowed with endogenous bioactive compounds. Their beneficial/health-promoting potential, together with enviro...
Article
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In Europe, pine forests are one of the most extended forests formations, making pine residues and by-products an important source of compounds with high industrial interest as well as for bioenergy production. Moreover, the valorization of lumber industry residues is desirable from a circular economy perspective. Different extraction methods and so...
Article
Rice bran (RB) is an important by-product from the rice production with a high valorization potential. The aim of the present work was to explore this potential by considering combined protein and phenolic fractions and enzymatic hydrolysis. The influence of different protocols in the recovery/fractioning of protein were evaluated, including defatt...
Article
Full-text available
Spirulina platensis is interesting for the food industry due to its overall composition and high content in C-phycocyanin. However, the sensitivity of C-phycocyanin makes its extraction a delicate process. The present study focuses on assessing the use of ohmic heating (OH) in the recovery of C-phycocyanin and other relevant compounds as an alterna...
Article
Full-text available
Natural compounds from agro-food by-products have fostered interest in food industries. The aim of this study was to unravel potential uses for Pinus pinaster bark extracts (PBE). As functional features of this type of extracts are usually attributed to phenolic compounds, the extraction process was studied. Different PBEs were achieved, with high...
Article
Concerns about fossil fuels depletion has led to seek for new sources of energy. The use of marine biomass (seaweed) to produce biofuels presents widely recognized advantages over terrestrial biomasses such as higher production ratio, higher photosynthetic efficiency or carbon-neutral emissions. In here, interesting seaweed sources as a whole or as...
Poster
Full-text available
Extracting the totality of bio-compounds with industrial interest from Cyanobacterium is often prevented by the intrinsic rigidity of its cell wall. In this sense, the present study focuses on evaluating the influence thermal batch extraction (conventional extraction technologies) and ohmic heating (OH) assisted extraction (considered a greener alt...
Chapter
Ohmic heating (OH) technology finds today several applications in innovative food processing. Its ability to provide a fast, direct, and volumetric heating brings several processing advantages at industrial scale regarding energetic efficiency and quality of food products. Over the last years the way how electric variables, such as electric field i...
Article
Pinus pinaster (pine) bark is a widely available wood industry by-product but an underexploited source of phenolic compounds with a strong antioxidant activity. The aim of this study was to evaluate the effectiveness of ohmic heating (OH, a recognized eco-friendly, electric fields-based processing technology) for the sustainable recovery of phenoli...
Article
Gracilaria tikvahiae, an endemic western North Atlantic red alga, was cultivated for nutrient bioextraction in urbanized estuarine waters in Long Island Sound and the Bronx River Estuary, USA. This study assesses the feasibility of an integrated approach of using G. tikvahiae produced in this bioextraction system as sustainable biomass source for a...
Article
Full-text available
Intensive aquaculture practices involve rearing fish at high densities. In these conditions, fish may be exposed to suboptimal dissolved O2 levels with an increased formation of reactive O2 species (ROS) in tissues. Seaweeds (SW) contain biologically active substances with efficient antioxidant capacities. This study evaluated the effects of dietar...
Article
The economic importance of fresh-cut fruit market is becoming progressively more significant, while the food industry shows increased interest in innovation of products bringing health benefits. The objective of this work was to assess the potential of incorporating ferulic acid (antioxidant with reported bioactivity that can act as cross-linking a...
Article
To attend the growing consumer demand for novel ready-to-eat fresh cut fruits packaging polylactic acid (PLA)-based active packaging was realized. The aim of these packaging is to provide an improved protection and even to extend their shelf-life. PLA-based active packaging was prepared by adding nanoclays and surfactants in its formulation. The ev...
Article
Locust bean gum synergistic interactions with whey proteins are widely described in terms of functional properties. The aim of this work is to assess how these interactions affect whey protein-based film properties. Blended films were manufactured using whey protein isolate (WPI), four different concentrations levels of locust bean gum (LBG) and tw...
Article
Abstract Determination of material properties and functions is a crucial step toward optimization of fabrication methods as well as the development of electrospun nanofibers for use in, e.g. food engineering applications. This work focused in evaluating physical and mass transfer properties of simple poly ɛ-caprolactone nanofibers (PCL membrane), a...
Conference Paper
Full-text available
The ability to promote nanoencapsulation within nanofibrous films for food packaging enables the possibility to have a double functionality: protection and bioactive compound dispensing. Aiming at a possible smart packaging material application, nanofibrous films from with encapsulated trypsin (PCL+tryp) were studied. Films' properties, structure a...
Presentation
Full-text available
Electrospun Nanofibers (ENf) find applications in several areas, such as biomedical, tissue engineering, pharmacology and also food engineering. One of the most important features is the ability of the ENf to serve as a filtration membrane as well as a catalytic surface. The pore size and the ability to encapsulate enzymes within the nanofibrous me...
Article
Full-text available
The aim of this work was to investigate the rheological behaviour of human blood, in a controlled stress rheometer, using different cone-plate and parallel plate geometries. Steady-shear (flow curves and kinetics) and oscillatory (strain, frequency and time sweeps) tests were performed at 37°C. The results demonstrated that rheological data are sig...
Article
The interaction with cervicovaginal mucus presents the potential to impact the performance of drug nanocarriers. These systems must migrate through this biological fluid in order to deliver their drug payload to the underlying mucosal surface. We studied the ability of dapivirine-loaded polycaprolactone (PCL)-based nanoparticles (NPs) to interact w...
Article
The replacement of conventional synthetic films and coatings by biodegradable alternatives reduces the use of non-renewable resources and waste disposal problems. Considering that Portugal is a major producer of leather, and consequently a large producer of related wastes, in this research, bovine hair was tested for the production of biodegradable...
Article
Chitosan packaging films containing different bioactive compounds (a peptide fraction from whey pro-tein concentrate (WPC) hydrolysate, glycomacropeptide (GMP) and lactoferrin) were produced and their mechanical and barrier properties were evaluated. The molecular weight of protein-based compounds was determined using SDS–PAGE. The addition of GMP...
Chapter
Full-text available
With increasing demand for sustainable materials, keratin wastes have been regarded, in recent years, as renewable resources worthy of exploitation. Each year, the Portuguese leather industry discards a considerable amount of animal hair, which constitutes a troublesome waste product. In this study, in an attempt to find a potential utilization for...
Chapter
Currently, mainly non-biodegradable petroleum-based synthetic polymers are used as packaging materials for foods, because of their availability, low cost and functionality. However, biodegradable/edible films can be made from polysaccharides, proteins and lipids without the environmental issues of petroleum-based polymers and with the additional ad...
Article
The aims of this work were to establish an efficient protocol for trypsin immobilization on spent grains and to assess the influence of the chosen protocol on whey protein hydrolysis. Trypsin was immobilized onto spent grain or modified spent grain, through adsorption and covalent attachment. The efficiency of immobilization and operation and stora...
Article
The synergistic interactions of two non-conventional galactomannans (Gleditsia triacanthos galactomannan (mannose/galactose ratio [M/G]=2.82) and Sophora japonica galactomannan (M/G=5.75)) with κ-carrageenan and xanthan were quantified and compared with those of two traditional galactomannans (guar gum—M/G=1.8 and locust bean gum—M/G=3.5) with the...
Article
The synergistic interactions of two non-conventional galactomannans (Gleditsia triacanthos galactomannan (mannose/galactose ratio [M/G] = 2 82) and Sophora japonica galactomannan (M/G = 5 75)) with kappa-carrageenan and xanthan were quantified and compared with those of two traditional galactomannans (guar gum M/G = 1 8 and locust bean gum M/G = 3...
Article
The gelling ability of whey proteins can be changed by limited hydrolysis and by the addition of other components such as polysaccharides. In this work the effect of the concentration of locust bean gum (LBG) on the heat-set gelation of aqueous whey protein hydrolysates (10%w/w) from pepsin and trypsin was assessed at pH 7.0. Whey protein concentra...
Article
The gelling ability of whey proteins can be changed by limited hydrolysis and by the addition of other components such as polysaccharides. In this work the effect of the concentration of locust bean gum (LBG) on the heat-set gelation of aqueous whey protein hydrolysates (10% w/w) from pepsin and trypsin was assessed at pH 7.0. Whey protein concentr...
Article
Full-text available
Topic: Multi-scale and/or multidisciplinary approach to process-product innovation. Abstract The gelling ability of whey proteins can be changed by limited hydrolysis and by the presence of other components such as polysaccharides; depending on the environmental conditions it can either be improved or impaired. In this work the effect of LBG on the...
Article
This study describes the characterisation of whey protein hydrolysates obtained from tryptic hydrolysis to assess their application as ingredients with angiotensin-converting-enzyme (ACE) inhibitory action. The levels of α-lactalbumin (α-la) and β-lactoglobulin (β-lg) remaining after hydrolysis were quantified. Peptides were separated by RP-HPLC, a...
Article
Hydrolysis of whey protein concentrates (WPCs) was performed using trypsin under different combinations of temperatures and pH values in a total of three experiments, namely experiment A at 37 °C and pH 8, experiment B at 37 °C and pH 9, and experiment C at 50 °C and pH 8. Monitorization of the degradation of native whey proteins and the peptide fo...
Article
Full-text available
Hydrolysis of whey protein concentrates (WPCs) was performed using trypsin under different combinations of temperatures and pH values in a total of three experiments, namely experiment A at 37 � C and pH 8, experiment B at 37 � C and pH 9, and experiment Ca t 50� C and pH 8. Monitorization of the degradation of native whey proteins and the peptide...
Conference Paper
Full-text available
Trypsin is a widely used enzyme for protein hydrolysis and can be used to improve functional and nutritional properties of foods. The immobilization of enzymes on solid supports can offer several advantages over free enzymes including easy handling, recovery from the reaction medium, reuse and operation in continuous reactors. Traditional carriers...
Article
Full-text available
Hydrolysis of whey protein concentrates (WPCs) at different temperatures and pHs, using three enzymes: pepsin, trypsin, and Alcalase, was monitored during more than 5 hr by reversed phase HPLC/UV, using a column containing a polystyrene‐divinylbenzene copolymer‐based packing, and an elution gradient from 8% to 80% acetonitrile containing 0.1% TFA....
Article
The main objective of this work is to elaborate methodologies that allow the identification of yield coefficients through complete measurements of the state. Experimental design strategies are proposed in order to optimize the richness of data coming out from the experiments, quantified by indexes related to the Fisher information matrix. The objec...
Article
Full-text available
This work compares different types of zeolites (NaA, NaX and NaY) as potential carriers for trypsin immobilisation. Silica was also used as a traditional carrier. Covalent attachment to the carriers was tested using glutaraldehyde. The efficiency of immobilisation and activity, operation and storage stability of free and immobilised enzyme on the t...

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Projects (5)
Project
O2HO will establish a technological platform for seaweed polysaccharides and bioactives extraction which is energetically more efficient, with higher selectivity, potentially with higher extraction yields, presumably producing lower amounts of residues. In the long term, this proof-of-concept can be extended to other matrices and different extracts.
Project
Technological Alliance to complete the forest and agroindustrial production cycle Reference SOE1/P1/E0123
Archived project
The main purpose of this project was to develop nanofibrous mats as enzyme carriers and separation membranes with improved performance in biocatalytic processes with interest in the agro-food area. The main approach was to develop attractive materials for enzyme immobilization, fibrous membranes prepared by electrospinning, with adequate structure and mechanical properties for easy reuse and permitting continuous processes, and high surface-to-volume ratio to enable adequate mass transfer and enzyme accessibility, having at the same time additional functionalities, namely acting as separation membranes.