Cristina Prat Aymerich

Cristina Prat Aymerich
Hospital Universitari Germans Trias i Pujol · Laboratory of Microbiology

MD, PhD

About

180
Publications
17,289
Reads
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4,197
Citations
Citations since 2017
71 Research Items
1691 Citations
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2017201820192020202120222023050100150200250300
2017201820192020202120222023050100150200250300
Additional affiliations
September 2007 - present
Autonomous University of Barcelona
Position
  • Professor (Associate)
Education
September 1990 - June 1996
Universitat de Lleida
Field of study
  • Medicine and Surgery

Publications

Publications (180)
Article
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Bacteria have the potential to translocate between sites in the human body, but the dynamics and consequences of within-host bacterial migration remain poorly understood. Here we investigate the link between gut and lung Pseudomonas aeruginosa populations in an intensively sampled ICU patient using a combination of genomics, isolate phenotyping, ho...
Article
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Background Current blood-based diagnostic tools for TB are insufficient to properly characterize the distinct stages of TB, from the latent infection (LTBI) to its active form (aTB); nor can they assess treatment efficacy. Several immune cell biomarkers have been proposed as potential candidates for the development of improved diagnostic tools. Ob...
Article
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Respiratory tract infections (RTI) are one of the commonest reasons for seeking healthcare, but are amongst the most challenging diseases in terms of clinical decision making. Proper and timely diagnosis is critical in order to optimize management and prevent further emergence of antimicrobial resistance by misuse, or overuse of antibiotics. Diagno...
Article
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Dual species interactions in co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization were examined. The genetic and phenotypic diversity between the isolates was high making the interactions detected strain-specific. Despite this, and the clinical origin of the strains, so...
Article
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An immunochemical strategy to detect and quantify AIP-IV, the quorum sensing (QS) signaling molecule produced by Staphylococcus aureus agr type IV, is reported here for the first time. Theoretical calculations and molecular modeling studies have assisted on the design and synthesis of a suitable peptide hapten (AIPIVS), allowing to obtain high avid...
Article
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This study investigated the evolution and epidemiology of the community-associated and multidrug-resistant Staphylococcus aureus clone European CC1-MRSA-IV. Whole-genome sequences were obtained for 194 European CC1-MRSA-IV isolates (189 of human and 5 of animal origin) from 12 countries, and 10 meticillin-susceptible precursors (from North-Eastern...
Article
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Tuberculosis (TB) is a major cause of morbidity and mortality in children, and early diagnosis and treatment are crucial to reduce long-term morbidity and mortality. In this study, we explore whether urine nuclear magnetic resonance (NMR)-based metabolomics could be used to identify differences in the metabolic response of children with different d...
Article
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: Staphylococcus aureus is a commensal and frequent colonizer of the upper respiratory tract. When mechanical ventilation disrupts natural defenses, S. aureus is frequently isolated from the lower airways, but distinguishing between colonization and infection is difficult. The objectives of this study were (1) to investigate the bacterial genome se...
Article
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Tuberculosis (TB) diagnosis is increasingly based on the detection of Mycobacterium tuberculosis complex (MTBC) DNA in sputum using molecular diagnostic tests as the first test for diagnosis. However, sputum can be difficult to obtain in children, patients without productive cough, and the elderly and approaches testing non-sputum samples are neede...
Article
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Despite efforts to improve tuberculosis (TB) detection, limitations in access, quality and timeliness of diagnostic services in low- and middle-income countries are challenging for current TB diagnostics. This study aimed to identify and characterise a metabolic profile of TB in urine by high-field nuclear magnetic resonance (NMR) spectrometry and...
Article
Background Smoking is a cause behind many diseases, including tuberculosis, and it is a risk factor for tuberculosis infection and mortality. Moreover, smoking is associated with a poor tuberculosis treatment outcome. Objectives In this study, we focus on the effects of cigarette smoke on an infected cell culture treated with anti-tuberculosis dru...
Article
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The accessory gene regulator (agr) locus of Staphylococcus aureus is a quorum-sensing virulence regulator. Although there are many studies concerning the effect of dysfunctional agr on the outcomes of S. aureus infection, there is no systematic review to date. We systematically searched for clinical studies reporting outcomes of invasive S. aureus...
Article
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Interferon (IFN)-γ release assays (IGRAs) are used to diagnose latent tuberculosis (TB) infection (LTBI). To improve the accuracy of these tests, different approaches, such as alternative cytokine detection and using different antigens, are considered. Following this purpose, this study aims to evaluate the addition of EspC, EspF and Rv2348-B to th...
Article
Interferon-gamma (IFN-γ) release assays (IGRAs) are increasingly used to test for latent TB infection. Although highly specific, IGRAs have a relatively high false-negative rate in active TB patients. A more sensitive assay is needed. IP-10 is an alternative biomarker with a 100 fold high expression level compared to IFN-γ, allowing for different a...
Article
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Health Care Workers (HCW) may have an occupational risk of latent tuberculosis infection (LTBI) and TB disease. The objective of this study was to evaluate the performance of the 2-step strategy: tuberculin skin test (TST) followed by confirmation with Interferon (IFN)-γ- release assays (IGRAs) in HCW. A secondary objective was to determine the fac...
Article
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Streptococcus pneumoniae is responsible for severe infections, causing millions of deaths yearly. Immunoglobulin G (IgG) antibodies against the capsular polysaccharide (CPS) offer S. pneumoniae serotype-specific protection. In this work, we examined the applicability of the microarray technology to detect CPS type-specific IgGs in serum, using a co...
Article
Aim: First, to compare in vitro minimum inhibitory concentrations (MIC) of free cloxacillin and cloxacillin-containing nanoparticles (NP) against methicillin susceptible (MSSA) and resistant Staphylococcus aureus (MRSA) and second, to assess NP antimicrobial activity against intracellular S. aureus. Methods: Poly(d,l-lactide-co-glycolide) acid (PLG...
Article
The multidrug-resistant Staphylococcus capitis NRCS-A clone is responsible for sepsis in preterm infants in neonatal intensive care units (NICUs) worldwide. Here, to retrace the spread of this clone and to identify drivers of its specific success, we investigated a representative collection of 250 S. capitis isolates from adults and newborns. Bayes...
Article
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A quarter of the global human population is estimated to be latently infected by Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB). TB remains the global leading cause of death by a single pathogen and ranks among the top-10 causes of overall global mortality. Current immunodiagnostic tests cannot discriminate between laten...
Article
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Multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) is an emerging threat to TB control in Ukraine, a country with the third highest XDR TB burden globally. We used whole-genome sequencing of a convenience sample to identify bacterial genetics and patient-related factors associated with MDR/XDR TB in this country. MDR/X...
Article
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Cigarette smoking and tuberculosis are a significant cause of death worldwide. Several epidemiological studies have demonstrated cigarette smoking is a risk factor for tuberculosis. Electronic cigarettes have recently appeared as a healthier alternative to conventional smoking, although their impact in tuberculosis is not well understood. The aim o...
Article
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The aim was to provide global experts ranking on priorities in diagnostic tools for VAP in clinical practice. A multiple criteria decision analysis (MCDA) was performed to identify diagnosis tools for VAP diagnosis. Priority factors were identified after literature review. An international, multidisciplinary expert panel reviewed variables and rank...
Article
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Objectives: To analyse all cases ofNocardia pneumonia occurring between 2010 and 2016 in five Spanish hospitals. Methods: Retrospective observational analysis of clinical and microbiological data collected from 55 cases ofNocardia pneumonia. Results: From 1 to 20 cases per hospital and from 6 to 9 cases per year were observed. COPD, bronchiect...
Article
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Tobacco smoking represents the leading preventable cause of death worldwide. Smoking is a recognised risk factor for several pathologies and is detrimental to host immune surveillance and defence. However, the impact of smoking on microbial residents of the nasopharyngeal cavity, in contact with cigarette smoke (CS), is lacking. Staphylococcus aure...
Article
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Objective: Streptococcus pneumoniae is the most frequent bacterial pathogen isolated in subjects with Community-acquired pneumonia (CAP) worldwide. Limited data are available regarding the current global burden and risk factors associated with drug-resistant Streptococcus pneumoniae (DRSP) in CAP subjects. We assessed the multinational prevalence...
Article
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Host susceptibility to respiratory tract infections (RTI) is dependent on both genetic and acquired risk factors. Repeated bacterial and viral RTI, such as pneumonia from encapsulated microorganisms, respiratory tract infections related to respiratory syncytial virus or influenza, and even the development of bronchiectasis and asthma, are often rep...
Article
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The aim of this study was to test the use of IP-10 detection in dried plasma from contact studies individuals (contacts of smear positive patients), by comparing it with IP-10 and IFN-γ detection in direct plasma, to establish IP-10 detection in DPS as a useful assay for LTBI diagnosis. Whole blood samples were collected from 80 subjects: 12 with a...
Article
Aim: Production of Matryoshka-type gastroresistant microparticles containing antibiotic-loaded poly lactic-co-glycolic acid (PLGA) nanoparticles (NP) against Mycobacterium tuberculosis. Materials & methods: The emulsification and evaporation methods were followed for the synthesis of PLGA-NPs and methacrylic acid-ethyl acrylate-based coatings to...
Article
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The immunological characterization of different cell markers has opened the possibility of considering them as immune tools for tuberculosis (TB) management, as they could correlate with TB latency/disease status and outcome. CD4⁺ T-cells producing IFN-γ⁺ with a low expression of CD27 have been described as an active TB marker. In addition, there a...
Data
Gating strategy used for CD4+/CD8+ T-cells cytokine secretion and CD27/CCR4 analysis. Aggregated cells were taken off by gating on the diagonal that appears with Forward-Scatter (height; FSC-H) vs. Forward-Scatter (area; FSC-A) dot plot. For CD4+ and CD8+ T-cells gating, alive CD3+ T-cells were first selected. Cytokine profiles on T-cells were anal...
Data
CD27− and/or CCR4+ phenotype within functional CD4+ T-cells producing IFN-γ and/or TNF-α in patients with active TB and LTBI individuals. Percentage of PPD or ESAT-6/CFP-10 specific CD27−, CCR4+, and CD27−CCR4+ within (A) TNF-α+CD4+ T-cells, (B) IFN-γ+TNF-α+CD4+ T-cells, and (C) IFN-γ+ and/or TNF-α+CD4+ T-cells. Horizontal lines represent medians....
Data
CD27 MFI ratio calculated on functional CD4+ T-cells producing IFN-γ and/or TNF-α. A ratio based on CD27 MFI was calculated after specific stimulation in active TB patients and LTBI individuals. This ratio is based on the MFI of CD27 in CD4+ T-cells over: (i) MFI of CD27 in TNF-α+CD4+ T-cells, (ii) MFI of CD27 in IFN-γ+TNF-α+CD4+ T-cells, and (iii)...
Data
Relationship of the CD27− expression on the different antigen-specific T-cells populations analyzed. Correlation of the CD27− expression on IFN-γ+CD4+ T-cells with (A) TNF-α+CD4+ or (B) TNF-α+IFN-γ+ CD4+ T-cells after PPD or ESAT-6/CFP-10 antigen stimulation. Correlation was calculated using the two-tailed non-parametric Spearman test.
Data
Correlation of homing markers expression with days of treatment. Correlation of days after starting anti-TB therapy in active TB patients (within the 4 weeks of starting treatment) with (A) percentage of CD27− and/or CCR4+ within IFN-γ+CD4+ T-cells in response to PPD or (B) ESAT-6/CFP-10 antigens. Correlation was calculated using the two-tailed non...
Article
Background: Inhaled antibiotics allow the delivery of higher drug concentrations at the site of infection without the systemic adverse effects observed with the use of parenteral or oral antibiotics. These antibiotics have shown to decrease the number of exacerbations, reduce bacterial load or improve pulmonary function in several chronic respirat...
Article
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This study aimed to describe real-life microbiological testing of adults hospitalised with community-acquired pneumonia (CAP) and to assess concordance with the 2007 Infectious Diseases Society of America (IDSA)/American Thoracic Society (ATS) and 2011 European Respiratory Society (ERS) CAP guidelines. This was a cohort study based on the Global In...
Article
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Highlights •Real life prevalence and risk factors for viral CAP depend on testing for viruses. •International testing frequency for viruses in patients with CAP is very low. •The most common cause of viral CAP is Influenza virus. •Risk factors for viral CAP are obesity and need for mechanical ventilation. •Globally, there is poor adherence to treat...
Article
Full-text available
This study aimed to describe real-life microbiological testing of adults hospitalised with community-acquired pneumonia (CAP) and to assess concordance with the 2007 Infectious Diseases Society of America (IDSA)/American Thoracic Society (ATS) and 2011 European Respiratory Society (ERS) CAP guidelines. This was a cohort study based on the Global In...
Article
Statement of significance: Medical-device associated infections present a great challenge in health care. Therefore, much research is undertaken to prevent bacterial colonization of new types of biomaterials. The work described here characterizes, tests and presents a number of clinically relevant bacterial model strains for assessing biofilm form...
Article
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Background Clinical accuracy of IGRAs remains unclear on patients with immune-mediated inflammatory diseases (IMIDs). Here, we assess the impact of immunosuppressants and IMIDs on QuantiFERON-TB Gold In-Tube (QFN-G-IT) and T-SPOT.TB accuracy. Methods Patients with IMIDs who required latent tuberculosis infection (LTBI) screening were enrolled and...
Article
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We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units – variable number tandem repeats (MIRU-VNTR...
Article
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Objectives To analyze whether the introduction of nebulized colistin in patients with chronic obstructive pulmonary disease (COPD) and infection with Pseudomonas aeruginosa (PA) is associated with a decrease of the number and duration of severe exacerbations. Materials and methods Thirty six patients with COPD and infection with PA treated with ne...
Article
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Objective: Staphylococcus aureus is a particularly difficult pathogen to eradicate from the respiratory tract. Previous studies have highlighted the intracellular capacity of S.aureus in a number of phagocytic and non-phagocytic cells. The aim of this study was to define S.aureus interaction within a murine alveolar macrophage cell line. Methods: C...
Article
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We evaluated Tuberculosis-Spoligo-Rifampicin-Isoniazid Typing (TB-SPRINT), a microbead-based method for spoligotyping and detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis. For that, 67 M. tuberculosis complex strains were retrospectively selected. Membrane-based spoligotyping, restriction fragment length polymorphism,...
Article
Objectives: Biologic agents are used against rheumatic diseases, however, they increase the risk of developing severe infections and diseases such as tuberculosis. We aimed to determine the benefits of IP-10 detection to diagnose latent tuberculosis infection (LTBI) in patients with inflammatory rheumatic diseases on different immunosuppressive dr...
Article
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Objective Characterization of Staphylococcus aureus clinical isolates derived from lower respiratory tract infections (LRTIs), and correlation between the functionality of the accessory gene regulator (Agr) and genotypic and phenotypic characteristics, clinical variables and clinical outcome. Methods S aureus isolates derived from LRTIs and contro...
Data
Measurement of RNAIII transcription by quantitative reverse transcribed RT-PCR. RNAIII transcripts of the 21 discordant strains were evaluated following RNA extraction at 6h. Fold change in RNAIII expression has been normalised to the positive control (RN6390B*) with negative control strain (RN6911) illustrated for comparison purposes. Red line den...
Data
Average fluorescence values obtained by the VLT according to the study group considered. Fluorescence values obtained by the VLT for the 148 strains analysed. (PDF)
Article
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Lower respiratory tract infections are among the top five leading causes of human death. Fighting these infections is therefore a world health priority. Searching for induced alterations in host gene expression shared by several relevant respiratory pathogens represents an alternative to identify new targets for wide-range host-oriented therapeutic...
Article
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Background MRSA is a therapeutic concern worldwide, and a major agent of community-acquired skin and soft tissue infections (CA-SSTIs). While the US epidemiology of MRSA in CA-SSTIs is well described and reports the high prevalence of the USA300 clone, data on the European situation are lacking. Objectives To determine the prevalence and clonal ch...