Cristina Airoldi

Cristina Airoldi
Università degli Studi di Milano-Bicocca | UNIMIB · Department of Biotechnology and Biosciences

PhD
Associate Professor of Organic Chemistry

About

136
Publications
23,092
Reads
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2,127
Citations
Introduction
My current scientific interests focus on: -NMR spectroscopy based characterization of biologically relevant molecular interactions and recognition processes, with also the support of other techniques, such as circular dicroism, surface plasmon resonance, mass spectrometry, IR spectroscopy and atomic force microscopy, - NMR spectroscopy based conformational analysis of bioactive compounds and -the application of NMR spectroscopy to the identification of metabolic markers of pathologies by metabolomics studies on cells and biofluids.
Additional affiliations
October 2019 - present
Università degli Studi di Milano-Bicocca
Position
  • Professor (Associate)
October 2016 - September 2019
Università degli Studi di Milano-Bicocca
Position
  • Professor (Assistant)
October 2012 - September 2016
Università degli Studi di Milano-Bicocca
Position
  • Researcher
Education
November 2003 - January 2007
October 1998 - November 2003

Publications

Publications (136)
Article
Full-text available
The anti-Alzheimer disease (AD) activity reported for an aqueous cinnamon bark extract prompted us to investigate and compare the anti-amyloidogenic properties of cinnamon extracts obtained from both bark and bud, the latter being a very little explored matrix. We prepared the extracts with different procedures (alcoholic, hydroalcoholic, or aqueou...
Article
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The anti-inflammatory activity of coffee extracts is widely recognized and supported by experimental evidence, in both in vitro and in vivo settings, mainly murine models. Here, we investigated the immunomodulatory properties of coffee extracts from green (GCE) and medium-roasted (RCE) Coffea canephora beans in human macrophages. The biological eff...
Chapter
Many biologically relevant molecular recognition processes are highly dynamic and rely on the equilibrium between ligands’ association and dissociation from their receptor(s), because of the reversible nature of the majority of them. Over the last few decades, several NMR experiments allowing the characterization of these interactions have been dev...
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The interaction of multi-LacNAc (Galβ1-4GlcNAc)-containing N-(2-hydroxypropyl) methacrylamide (HPMA) copolymers with human galectin-1 (Gal-1) and the carbohydrate recognition domain (CRD) of human galectin-3 (Gal-3) was analyzed using NMR methods in addition to cryo-electron-microscopy and dynamic light scattering (DLS) experiments. The interaction...
Article
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Proteasome inhibitors (PIs) represent the gold standard in the treatment of multiple myeloma. Among PIs, Bortezomib (BTZ) is frequently used as first line therapy, but peripheral neuropathy (PN), occurring approximately in 50% of patients, impairs their life, representing a dose-limiting toxicity. Carfilzomib (CFZ), a second-generation PI, induces...
Article
Peucedanum ostruthium (L.) W.D.J.Koch (Apiaceae) is an alpine medicinal plant traditionally used as a panacea to treat various ailments. For the first time, its phytotoxic and nematotoxic properties were investigated. The inhibitory activity toward germination and seedling growth of the weeds Echinochloa oryzoides (Ard.) Fritsch and Lolium multiflo...
Article
We describe the development of an on-cell NMR method for the rapid screening of FimH ligands and the structural identification of ligand binding epitopes. FimH is a mannose-binding bacterial adhesin expressed at the apical end of type 1 pili of uropathogenic bacterial strains and responsible for their D-mannose sensitive adhesion to host mammalian...
Article
We report the rational design, synthesis, and in vitro preliminary evaluation of a new small library of non-peptide ligands of Gastrin Releasing Peptide Receptor (GRP-R), able to antagonize its natural ligand bombesin (BN) in the nanomolar range of concentration. GRP-R is a transmembrane G-protein coupled receptor promoting the stimulation of canc...
Article
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Ras oncoproteins play a crucial role in the onset, maintenance, and progression of the most common and deadly human cancers. Despite extensive research efforts, only a few mutant-specific Ras inhibitors have been reported. We show that cmp4-previously identified as a water-soluble Ras inhibitor-targets multiple steps in the activation and downstrea...
Chapter
Substitution with a carbon or a sulfur atom of the oxygen that in saccharides links one sugar unit to another sugar or to an aglycon generates compounds defined as C-glycosides and S-glycosides, respectively. The interest in these classes of compounds lies in the metabolic stability of the glycosidic linkage, as useful characteristic for potential...
Article
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Palmitoylethanolamide (PEA) belongs to the class of N-acylethanolamine and is an endogenous lipid potentially useful in a wide range of therapeutic areas; products containing PEA are licensed for use in humans as a nutraceutical, a food supplement, or food for medical purposes for its analgesic and anti-inflammatory properties demonstrating efficac...
Article
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NF-Y is a transcription factor (TF) comprising three subunits (NF-YA, NF-YB, NF-YC) that binds with high specificity to the CCAAT sequence, a widespread regulatory element in gene promoters of prosurvival, cell-cycle-promoting, and metabolic genes. Tumor cells undergo “metabolic rewiring” through overexpression of genes involved in such pathways, m...
Article
The metabolic profile of Lavado cocoa was characterized for the first time by NMR spectroscopy, then compared with the profiles of fermented and processed varieties, Natural and commercial cocoa. The significant difference in the contents of theobromine and flavanols prompted us to examine the cocoa varieties to seek correlations between these meta...
Article
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Rice cultivation, particularly prone to weed issues, requires practices able to effectively control them, however reducing the use of herbicides, responsible for damage to human health and ecosystem sustainability. Alternative strategies for weed management can be based on plant-plant interaction phenomena. In this context, a group of organic farme...
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Colorectal cancer (CRC) is one of the most common types of cancer, especially in Western countries, and its incidence rate is increasing every year. In this study, for the first time Vigna unguiculata L. Walp. (cowpea) water boiled seed extracts were found to reduce the viability of different colorectal cancer (CRC) cell lines, such as E705, DiFi a...
Article
The authentication and traceability of craft beers is an important issue for both beer consumers and producers. Reliable analytical methods able to identify and discriminate products are needed to protect the craft brew market against fraud and counterfeit. Here, 1H-NMR analysis of 31 beer samples, differing for beer style and brewing method (craft...
Article
We report the NMR characterization of the molecular interaction between Gastrin Releasing Peptide Receptor (GRP-R) and its natural ligand bombesin (BN). GRP-R is a transmembrane G-protein coupled receptor promoting the stimulation of cancer cell proliferation; in addition, being overexpressed on the surface of different human cancer cell lines, it...
Article
Nuclear Magnetic Resonance (NMR) is an analytical technique extensively used in almost every chemical laboratory for structural identification. This technique provides statistically equivalent signals in spite of using spectrometer with different hardware features and is successfully used for the traceability and quantification of analytes in food...
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Mint [Mentha longifolia (L.) Hudson] is an aromatic plant that belongs to Lamiaceae family. It is traditionally used as herbal tea in Europe, Australia and North Africa and shows numerous pharmacological effects, such as spasmolytic, antioxidant, antimicrobial and anti-hemolytic. Recently, its antiproliferative role has been suggested in a small nu...
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Spectroscopic non-targeted methods are gaining ever-growing importance in quality control and authenticity assessment of food products because of their strong potential for identification of specific features of the products by data-driven classifiers. One of the factors hampering the diffusion of spectroscopic non-targeted methods and data-driven...
Article
Biofilm formation by bacterial pathogens is a hallmark of chronic infections and is associated to increased antibiotic tolerance that makes pathogens difficult to eradicate with conventional antibiotic therapies. Infections caused by Pseudomonas aeruginosa are of great concern, especially for immunocompromised and cystic fibrosis patients. P. aerug...
Article
The growing interest in medicinal plants for the identification of new bioactive compounds and the formulation of new nutraceuticals and drugs prompted us to develop a powerful experimental approach allowing the detailed metabolic profiling of complex plant extracts, the identification of ligands of macromolecular targets of biomedical relevance an...
Article
Carbohydrates are one of the most powerful and versatile classes of biomolecules that nature uses to regulate organisms biochemistry, able to regulate plenty of signaling events within cells, triggering a plethora of physiological and pathological cellular behaviors. In this framework, glycan carrier systems or carbohydrate-decorated materials cons...
Article
Mitochondria play essential metabolic functions in eukaryotes. Although their major role is the generation of energy in the form of ATP, they are also involved in maintenance of cellular redox state, conversion and biosynthesis of metabolites and signal transduction. Most mitochondrial functions are conserved in eukaryotic systems and mitochondrial...
Article
Background We have previously demonstrated the neuroprotective activity of tetracycline on a Spinocerebellar Ataxia 3 nematode model. Here, we present the screening of a small library of tetracycline congeners in order to identify the most effective compound in preventing ataxin-3 aggregation. Methods We performed the assays on the Josephin Domain...
Article
The structure-activity relationship was investigated in a series of synthetic TLR4 antagonists formed by a glucosamine core linked to two phosphate esters and two linear carbon chains. Molecular modeling showed that the compounds with 10, 12 and 14 carbons chains are associated to higher stabilization of the TLR4/MD-2 antagonist conformation than i...
Article
Flavonoids are phytochemicals present in almost all terrestrial plants and, as a consequence, in plant-based foods, and thus consumed by humans through the diet. Recent evidences suggest that several flavonoids have positive effects against dementia and Alzheimer's disease, reversing age-related declines in neurocognitive performances. In this revi...
Article
To identify food and beverages that provide the regular intake of natural compounds capable of interfering with toxic amyloidogenic aggregates, we developed an experimental protocol that combines NMR spectroscopy and atomic force microscopy, in vitro biochemical and cell assays to detect anti-Aβ molecules in natural edible matrices. We applied thi...
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Full-text available
Human immunodeficiency virus type-1 (HIV-1)-associated neurocognitive disorder (HAND) remains an important neurological manifestation that adversely affects a patient’s quality of life. HIV-1 matrix protein p17 (p17) has been detected in autoptic brain tissue of HAND individuals who presented early with severe AIDS encephalopathy. We hypothesised t...
Article
Recent epidemiological studies demonstrate that consumption of healthy foods, especially rich in polyphenols content, might reduce the incidence of cancer and degenerative diseases. In particular, chlorogenic acids (CGAs) occur ubiquitously in food, representing the most abundant polyphenols in the human diet. A number of CGA beneficial biological...
Article
The protein ataxin-3 (ATX3) triggers an amyloid-related neurodegenerative disease when its polyglutamine stretch is expanded beyond a critical threshold. We formerly demonstrated that the polyphenol epigallocatechin-3-gallate (EGCG) could redirect amyloid aggregation of a full-length, expanded ATX3 (ATX3-Q55) towards non-toxic, soluble, SDS-resista...
Article
Nutritional factors play a pivotal role for healthy aging and longevity. This is related to the antioxidant properties of the molecules present in some foods. Due to the high content of polyphenols, Green Coffee Extract (GCE) is a powerful antioxidant. Nevertheless, little is known about its effect on aging. We demonstrated the benefic effects of G...
Article
This report describes the application of NMR spectroscopy to the profiling of metabolites in BronchoAlveolar Lavage fluid (BALf) of lung transplant recipients without Bronchiolitis Obliterans Syndrome (BOS) (stable, S, n=10), and with BOS at different degree of severity (BOS 0p, n=10; BOS I, n=10). Through the fine tuning of a number of parameters...
Article
Combining NMR spectroscopy, transmission electron microscopy, biochemical and in vitro toxicity assays, we characterized the effect of flavonoid glycosylation, a chemical modification very frequent in Nature, on their ability to recognise and bind Aβ1-42 oligomers, preventing their aggregation and their neurotoxicity. Our data allow the elucidation...
Article
The metabolomic analysis of Exhaled Breath Condensate (EBC) may provide insights on both the pathology of pulmonary disorders and the response to therapy. This pilot study describes the ability of Nuclear Magnetic Resonance (NMR)-based metabolomics to discriminate α1-antitrypsin deficient (AATD)-patients who were diagnosed with moderate to severe e...
Article
Purpose: Tools that will help unraveling the complexity of Bronchiolitis Obliterans Syndrome (BOS) and identifying useful predictive markers are urgently needed. Given its ability of capturing disease-relevant metabolic profile changes, Metabolomics may occupy an important role in this area. Methods: BALf was obtained fromlung transplant patients,...
Article
Full-text available
Cancer cells often rely on glycolysis to obtain energy and support anabolic growth. Several studies showed that glycolytic cells are susceptible to cell death when subjected to low glucose availability or to lack of glucose. However, some cancer cells, including glycolytic ones, can efficiently acquire higher tolerance to glucose depletion, leading...
Article
Full-text available
Cancer cells often rely on glycolysis to obtain energy and support anabolic growth. Several studies showed that glycolytic cells are susceptible to cell death when subjected to low glucose availability or to lack of glucose. However, some cancer cells, including glycolytic ones, can efficiently acquire higher tolerance to glucose depletion, leading...
Data
The genes regulated by FSK in Transformed cells show a low degree of connection. In the figure the network of predicted associations for all DEGs-encoded proteins in TF/T comparison is shown. The STRING analysis of the protein-protein interactions was performed to DEGs with fold change ≥2 in the comparison. (PDF)
Data
The treatment with FSK induces a relevant change in the expression of genes related to the glutamine metabolism. Transcriptional data from microarray analysis regarding glutamine metabolism-related genes in Transformed cells at 72h of culture in LG, daily treated with DMSO or FSK. Data express the ratio in TF/T comparison. (PDF)
Data
FSK-treated MDA-MB-231 cells show a different expression of glutamine metabolism-related genes as compared to untreated cells. The expression of genes related to the glutamine metabolism was performed in MDA-MB-231 cells daily treated with DMSO or FSK after 48h and 72h of cultivation in LG. mRNA expression of glutamine metabolism-related genes was...
Data
PKA and autophagy activation in suspended MDA-MB-231 cells. A MDA-MB-231 cells treated with FSK were collected and stained with fluorescent substrate of the active caspase-3 and then newly attached to the coverslip using cytospin. Then they were fixed and labeled with the antibody for PKA substrates. The images were visualized by using fluorescent...
Data
Genes and proteins identified in our study and used for the Gene set enrichment analysis. Transcriptional and proteomic data have been mapped in the 202 KEGG pathways (metabolic and signaling). (XLS)
Data
Graphical representation of the experimental workflow -/+FSK. A, D For the analyses upon treatment with FSK Normal and Transformed cells (A) as well as MDA-MB-231 (D) were cultured in LG medium (1mM as initial concentration) 16h after seeding. Then, starting from 24h the cells were daily treated with DMSO (vehicle) or 10μM FSK. When required, at 72...
Data
The genes regulated by FSK in Normal cells show a high degree of connection. In the figure the network of predicted associations for all DEGs-encoded proteins in NF/N comparison is shown. The STRING analysis of the protein-protein interactions was performed to DEGs with fold change ≥2 in the comparison. (PDF)
Data
Network of predicted associations for all the differentially expressed proteins identified by 2-DIGE. The STRING analysis of the protein-protein interactions was performed using proteins with spot variation ≥10% in NF/N (A) and TF/T (B) comparisons. (PDF)
Data
The inhibition of PKA prevents the resistance to glucose deprivation and anoikis. A Graphical representation of the experimental workflow for analyses in anoikis-resistant floating cells. MDA-MB-231 cells were cultured in LG. At 72h, when a significant part of the cell population was detached from the plate, the suspended cells were collected and t...
Data
PKA pathway is endogenously activated in Transformed cells cultured in glucose deprivation, mediating their resistance to anoikis. A At indicated time points of culture in LG, floating cells were stained with trypan blue and counted to verify whether floating cells were resistant to anoikis. Percentage of floating and dead cells was reported over t...
Data
Glucose depletion induces low anoikis-resistance in MIA PaCa-2 when cultivated on standard support. A MIA PaCa-2 cells were cultured in LG (1mM glucose as initial concentration). The amount of glucose in the medium was measured using an enzymatic kit. B-C MIA PaCa-2 cells cultured in HG and LG were counted at different time points with Trypan blue,...
Data
Highly differentially expressed genes in the two comparisons NF/N and TF/T. The two spreadshits represent the genes with logFC > = 1 in the two comparisons NF/N and TF/T and shown in the heatmap of Fig 1B (XLS)
Data
List of primers used for qPCR amplification of target genes. Table A: Normal and Transformed cells. Table B: MDA- MB-231 cells (PDF)
Data
Analysis of transcriptomic and proteomic data using PIANO method. The heatmap shows the result obtained by applying the PIANO tool to gene (A) and protein (B) datasets separately. In particular, the top 10-ranked pathways associated to each comparison, NF/N and TF/T, are shown. The different color of the heatmap represents the rank position of the...
Data
The FSK treatment attenuates UPR in both Normal and Transformed cells. The analysis shown here was performed in cells cultured for 72h in LG and daily treated with DMSO or 10μM FSK. A-B mRNA expression of UPR-related genes was analyzed by qPCR for Transformed (A) and Normal (B) cells. mRNA expression levels in FSK-treated cells are reported as chan...
Data
The induction of the PKA pathway mediates the autophagy activation in Transformed cells in glucose deprivation. A PKA activation was evaluated by Western blot analysis of p-(Ser/Thr) PKA substrates and pCREB S133 in Transformed cells daily treated with FSK and/or 2μM H89. B The cellular morphology of the cells -/+ FSK and -/+H89 was observed at 96h...
Data
FSK-protective role is not mediated by Epac. A Schematic representation of FSK-mediated activation of PKA and Epac proteins upon intracellular cAMP enhancement. To analyze the effect of Epac inhibition, the cells were daily treated with FSK and/or 1μM Epac inhibitor (ESI-09). B Cells -/+FSK and -/+ESI-09 were counted with trypan blue at 72h of cult...
Data
PKA activation protects MIA PaCa-2 pancreatic cancer cells by glucose deprivation-induced cell death. A-H For the analyses of -/+FSK MIA PaCa-2 cells were cultured in LG and daily treated with DMSO or FSK. A Microscopy images of cell morphology. B PKA activity after FSK treatment was evaluated by Western blot analysis of p-(Ser/Thr) PKA substrates...
Data
Proteins identified by mass spectrometry in the two comparisons NF/N and TF/T. The two spreadsheets show the mass spectrometry data, the UniProtKB accession number and percentage of the volume spot variation. (XLS)
Data
Total gene expression analysis. The table show the 17669 transcripts used for PIANO analysis and represented as hetamap in Fig 1C. N: Normal cells; N+F: Normal cells + FSK; T: Transformed cells; T+F: Transformed cells + FSK. (XLS)
Data
The inhibition of PKA counteracts the protective effects of FSK in MDA-MB-231. MDA-MB-231 cells were analyzed upon daily treatment with FSK and 2μM H89. A PKA activation by Western blot analysis of p-(Ser/Thr) PKA substrates and pCREB S133. B Microscopy images of the cells were collected at 72h of culture. C Western blot analysis of Grp78 and CHOP...
Data
The table reports the rankings obtained by each KEGG pathway (rows) in the two comparisons (N+F vs N and T+F vs T) in the transcript, in the proteomic, and in the integrated/aggregated dataset for the three directionality classes (down- and up-regulation, and non-directional regulation). Pathways in the first 10 positions for the transcript and int...
Data
Pathways used for Venn diagram. (XLS)