Cristian Araneda

University of Chile · Departamento de Producción Animal

The immune response of Atlantic salmon to sea lice has been extensively studied, but we still do not know the mechanisms by which some fish become resistant and others do not. In this study, we estimated the heritabilities of three key proteins associated with the innate immunity and resistance of Salmo salar against the sea louse Caligus rogercres...

RESUMEN La pandemia del SARS-CoV-2 ha creado un desafío sin precedentes para la educación. En 2020, como resultado de las medidas para reducir la propagación del SARS-CoV-2, muchas instituciones educativas pasaron del aprendizaje presencial al aprendizaje remoto. Sin embargo, este modelo educativo puede generar estrés y ansiedad adicionales a la an...

Seafood international trade has increased the labeling requirements in standards and regulations to include product information that enable traders and consumers to make informed choices. The European Union (EU) Regulation No. 1379/2013 imposes the declaration of an official commercial designation and scientific names for all the fishery and aquacu...

Clams represent a very important marine resource for Chile with an annual production during 2021 of 21,932 tons. The main commercial species are Ameginomya antiqua and Protothaca thaca, both fisheries in a state of full exploitation, followed by Tawera elliptica. Other species of commercial importance are Mulinia edulis, Semele solida, Gari solida,...

Background Approximately 30% of individuals with schizophrenia (SZ) are resistant to conventional antipsychotic drug therapy (AP). Of these, one third are also resistant to the second-line treatment, clozapine. Treatment resistance and refractoriness are associated with increased morbidity and disability, making timely detection of these issues cri...

Food fraud and the conservation and trade of threatened species are topics of high concern. Some shark species are considered in this category by IUCN and CITES, and can enter as mislabeled or substituted in the commercialization chain. In Chile, three shark species are present as by-catch in the swordfish (Xiphias gladius) fisheries; these are sol...

Mussels of the Mytilus genus or blue mussels are cultured extensively worldwide. According to FAO statistics in 2019, the main cultured species in order of annual production are M. chilensis, M. edulis and Mytilus galloprovincialis. Due to their morphological similarity, blue mussels may be incorrectly assigned to the species leading to the occurre...

Abalone is a marine gastropod mollusk that belongs to the genus Haliotis -high-priced and with commercial importance worldwide. Because of its high demand, its products are susceptible to retail fraud by substituting lower-value products or protected species instead of higher-value ones. Therefore, species identification is a crucial traceability e...

A male specimen of the Aesop slipper lobster Scyllarides haanii was found in the coastal vicinity of Ahu Tongariki (27°07'33"S, 109°16'37"W), Rapa Nui. Its taxonomic determination was carried out by a detailed morphological examination and a DNA barcoding identification analysis by sequencing the cytochrome oxidase subunit 1 (COX1) gene. S. haanii...

The European aquaculture industry began to undertake Atlantic halibut (Hippoglossus hippoglossus) aquaculture in the 1980s due to the favorable growth rate and high market value of this cold-water marine fish. Atlantic halibut cultivation began in Chile in 1996 in the Magallanes region (53˚S). Chilean aquaculture facilities were finally equipped to...

Seafood traceability represents a major goal for regulators and fishing industries worldwide who seek to prevent commercial fraud, protect marine resources and ensure consumer safety. Genetic approaches can be used to trace the provenance of seafood based on the ability of DNA markers to assign samples back to their population of origin. Here, we h...

Sturgeons are fish commercially important for caviar production. They are endangered in the wild due to overfishing, river pollution, and human action. We have generated a high-quality > 74 K SNP panel from female and male Acipenser baerii using a high-depth RAD-seq approach. This SNP panel will facilitate many types of genetic studies, including h...

The labelling compliance with EU and national legislation of market products composed of Mytilus spp. sold in Italy was evaluated. Then, a PCR-RFLP technique analyzing a region of the Polyphenolic Adhesive Protein (PAP) nuclear gene was used to support the label assessment. The technique allows to discriminate the species most commercialized in EU...

The sexual maturation of Atlantic salmon Salmo salar is a multifactorial process in which fish acquire somatic characteristics to reproduce. In salmon farming has been described a high variability in the trait age at maturation derived from wild reproductive strategies. Early maturation is a phenotype that generates serious economic repercussions o...

DNA-based methods using informative markers such as single nucleotide polymorphism (SNPs) are suitable for reliable species identification (SI) needed to enforce compliance with seafood labelling regulations (EU No.1379/2013). We developed a panel of 10 highly informative SNPs to be genotyped by PCR-High resolution melting (HRM) for SI in the Mytil...

The molecular mechanisms underlying fish tolerance to soybean meal (SBM) remain unclear. Identifying these mechanisms would be beneficial, as this trait favors growth. Two fish replicates from 19 experimental families were fed fishmeal-(100FM) or SBM-based diets supplemented with saponin (50SBM + 2SPN) from juvenile to adult stages. Individuals wer...

The yeast Saccharomyces cerevisiae is the main species responsible for the process that involves the transformation of grape must into wine, with the initial nitrogen in the grape must being vital for it. One of the main problems in the wine industry is the deficiency of nitrogen sources in the grape must, leading to stuck or sluggish fermentations...

Mytilus mussels have been the object of much research given their sentinel role in coastal ecosystems and significant value as an aquaculture resource appreciated for both, its flavour and nutritional content. Some of the most-studied Mytilus species are M. edulis, M. galloprovincialis, M. chilensis and M. trossulus. As species identification based...

The red cusk-eel (Genypterus chilensis, Guichenot, 1848) is an iconic species in Chilean gastronomy, with a cultural significance that extends beyond its economic value, worthy of conservation. Despite the decline in fishery productivity across most of the distribution range for this species, little effort has been devoted to assessing stock manage...

Anímense a descargar el libro gratuito de Genética: Conceptos básicos de Genética. Libro de ejercicios. Disponible en la página de la UNAM: http://www.librosoa.unam.mx/handle/123456789/2003

“En el sistema de AI esporofítico, el “fenotipo de identificación” de los granos de polen está determinado por el genotipo diploide de la planta que los produce. La determinante S del polen se expresa en el tapetum, tejido esporofítico (2n) de la pared de la “antera". En contraste a este sistema, en la AI gametofítica es el fenotipo haploide de los...

El presente libro es el resultado de la colaboración de 25 académicos de México, Argentina, Chile y España. Este esfuerzo colegiado cubre los temas principales de un curso de Genética para estudiantes de la licenciatura en Biología y profesiones afines. Cada tema contiene una pequeña y rigurosa introducción, así como series de problemas a resolver,...

Findings regarding sex‐linked markers and the sex determining gene have important implications in salmonid production, where all‐female stocks are preferable. Producing a monosex female population increases commercial yield, and avoids many of the problems associated with precocious maturation of males. We review the most relevant molecular markers...

Salmonids have two sex hormone-binding globulin (Shbg) paralogs. Shbga is mainly expressed in the liver, while Shbgb is secreted by the granulosa cells of the rainbow trout ovary. Coexpression of shbgb and the gonadal aromatase cyp19a1a mRNAs been observed in granulosa cells, suggesting a physiological coordination between Shbgb expression and estr...

Gaining new knowledge of the native distributions of species (phylogeography) is more and more difficult in a world affected by anthropogenic disturbance, in particular by species translocations. Increasingly, molecular markers are required to support decisions about the taxonomy of native versus introduced species, and the existence of their hybri...

Best use of scientific knowledge is required to maintain the fundamental role of seafood in human nutrition. While it is acknowledged that genomic-based methods allow the collection of powerful data, their value to inform fisheries management, aquaculture, and biosecurity applications remains underestimated. We review genomic applications of releva...

The Patagonian toothfish or Chilean sea bass (Dissostichus eleginoides), found in the Southern Ocean surrounding Antarctica, is an important fishery species for Chile. This high-value species is regarded as overfished, making it an attractive target for aquaculture. When developing a reproduction program for any aquaculture species, it is important...

Chilean mussel populations have been thought to be panmictic with limited genetic structure. Genotyping-by-sequencing approaches have enabled investigation of genomewide variation that may better distinguish populations that have evolved in different environments. We investigated neutral and adaptive genetic variation in Mytilus from six locations...

Mussels (Mytilus spp.) are one of the most cultivated and commercialized bivalves in southern Chile; culture is currently supplied almost entirely from wild-caught seed obtained from relatively few collection centers. The genetic diversity and differentiation of the blue mussel in southern Chile was investigated by sampling six locations: one natur...

The objectives of this study were to measure gene expression in zebrafish and then identify SNP to be used as potential markers in a growth association study. We developed an approach where muscle samples collected from low- and high-growth fish were analyzed using RNA-Sequencing (RNA-seq), and SNP were chosen from the genes that were differentiall...

In spite of potentially being an important source of rhizobial diversity and a key determinant of common bean productivity, there is a paucity of data on Rhizobium genetic variation and species composition in the important bean producing area of Chile and only one species has been documented (Rhizobium leguminosarum). In this study, 240 Rhizobium i...

Appearance traits in fish, those external body characteristics that influence consumer acceptance at point of sale, have come to the forefront of commercial fish farming, as culture profitability is closely linked to management of these traits. Appearance traits comprise mainly body shape and skin pigmentation. Analysis of the genetic basis of thes...

Abstract The main objective of this study was to examine the effects of a plant protein- vs. fishmeal-based diet on growth response in a population of 24 families, as well as expression of growth-related genes in the muscle of adult zebrafish (Danio rerio). Each family was split to create two fish populations with similar genetic backgrounds, and t...

This study assessed the relative contributions of host genetics and diet in shaping the gut microbiota of rainbow trout. Full sibling fish from four unrelated families, each consisting of individuals derived from the mating of one male and one female belonging to a breeding program, were fed diets containing either vegetable proteins or vegetable o...

This study compares the gonadosomatic index (GSI), oocyte growth (OG), gonadal histology, and plasma level concentrations of sex hormones (estradiol-17β (E2) and vitellogenin (V)) of twice-spawning (T-SP) and once-spawning (O-SP) females of rainbow trout throughout the additional and the normal reproductive cycle, respectively. In T-SP, the GSI val...

Mussels are one of the most cultivated and commercialized bivalves worldwide and in southern Chile its culture represent an important economic activity. The species identification within the Mytilus genera, by morphological features, is unreliable, so we used a polymorphism RFLP in the gene encoding the polyphenolic adhesive protein as a species-sp...

In trout farming males exhibits lower growth rate and precocious sexual maturation in contrast to females. Since these traits are detrimental for intensive production, all females populations are most appreciated by fish farmers. Molecular markers sex-specific have been developed for rainbow trout that can be useful to diagnose the phenotypic sex o...

In trout farming males exhibits lower growth rate and precocious sexual maturation in contrast to females. Since these traits are detrimental for intensive production, all females populations are most appreciated by fish farmers. Molecular markers sex-specific have been developed for rainbow trout that can be useful to diagnose the phenotypic sex o...

Spawning time in salmonids is a sex-limited quantitative trait that can be modified by selection. In rainbow trout (Oncorhynchus mykiss), various quantitative trait loci (QTL) that affect the expression of this trait have been discovered. In this study, we describe four microsatellite loci associated with two possible spawning time QTL regions in c...

This study assessed the relative contributions of host genetics and diet in shaping the gut microbiota of rainbow trout. Full sibling fish from four unrelated families, each consisting of individuals derived from the mating of one male and one female belonging to a breeding program, were fed diets containing either vegetable proteins or vegetable o...

Total bacterial counts (Log10+/−SE) in the intestinal contents of the rainbow trout ( Oncorhynchus mykiss ), as determined by epifluorescence microscopy. (DOC)

Distal intestinal epithelia of the rainbow trout (Oncorhynchus mykiss) in this study. Fish were fed on the following diets: (A) control diet D1, where 100% of the protein in the diet was provided by fish meal and 100% of the oil was provided by fish oil; (B) diet D2, where 50% of the protein in the diet was provided by fish meal and 50% was provide...

Relative abundances of the different phyla identified. Phyla identified in (A) the four unrelated trout (Oncorhynchus mykiss) families (F1, F2, F3 and F4) fed (B) the three diets (D1, D2 and D3). (DOC)

Distribution of the rainbow trout ( Oncorhynchus mykiss ) included in this study. (DOC)

Semi-quantitative scoring system for the different parameters used to assess the degree of enteritis as described by Urán et al., (2009). The following parameters are analyzed: the changes in the morphology of the mucosal folds (MF) and supranuclear vacuoles (SNV), the abundance of goblet cells (GC), the degree of infiltration of eosinophilic granu...

Average value of the enteritis parameters scored using the semi-quantitative scoring system (average+/−standard error) as described in Table S2. The following parameters were analyzed: the changes in the morphology of the mucosal folds (MF) and supranuclear vacuoles (SNV), the abundance of goblet cells (GC), the degree of infiltration of eosinophil...

Zebrafish (Danio rerio) is a common research model in fish studies of toxicology, developmental biology, neurobiology and molecular genetics; it has been proposed as a possible model organism for nutrition and growth studies in fish. The advantages of working with zebrafish in these areas are their small size, short generation time (12–14weeks) and...

The rainbow trout is a salmonid fish that occasionally exhibits broodstocks with biannual spawning behavior, a phenomenon known as a double annual reproductive cycle (DARC). Spawning time quantitative trait loci (SPT-QTLs) affect the time of the year that female rainbow trout spawn and may influence expression of the DARC trait. In this study, micr...

In salmonids, spawning date is an important productive and life-history trait determining the time of fertilization, date of progeny emergence, and even the probability of survival and the growth rate of the small fry. Recent evidences support a polygenic basis with several Quantitative Trait Loci (QTL) affecting the phenotypic expression of this t...

In the last decade, advances in molecular methods have permitted the detection of major genes or chromosomal regions (quantitative trait loci, QTLs) that account for an important fraction of the phenotypic variance of productive traits in many domestic animals, principally mammals. These discoveries have only been possible due to the development of...

Flesh colour in salmonids is a very important commercial trait that shows additive genetic variance with significant environmental influence. Selective breeding for this trait is difficult, since phenotypic evaluation requires individuals to be sacrificed for scoring. We have identified a RAPD marker that co-segregates with colour traits scored as...

Phenotypic and genetic parameter estimates of carcass quality traits were obtained from two pedigreed populations, termed even and odd, of a Coho salmon breeding program. Carcass quality data from 3444 fish (1802 male and 1642 female), harvested at 21 months of age, were analyzed. In both populations, significant sex effects were found for body wei...