Coralia Bleotu

Coralia Bleotu
Institute of Virology, Romanian Academy, Romania · Cellular and Molecular Pathology Department

About

311
Publications
51,309
Reads
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3,782
Citations
Additional affiliations
October 2014 - present
University of Bucharest
Position
  • Associate Prof. (partial)
March 1998 - present
Stefan S. Nicolau Institute of Virology (IVN)
Position
  • SRII

Publications

Publications (311)
Article
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Antimicrobial and anticancer drug resistance represent two of the main global challenges for the public health, requiring immediate practical solutions. In line with this, we need a better understanding of the origins of drug resistance in prokaryotic and eukaryotic cells and the evolutionary processes leading to the occurrence of adaptive phenotyp...
Article
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This paper aimed to develop two types of support materials with a mesoporous structure of mobile crystalline matter (known in the literature as MCM, namely MCM-41 and MCM-48) and to load them with gallic acid. Soft templating methodology was chosen for the preparation of the mesoporous structures—the cylindrical micelles with certain structural cha...
Article
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The efficient regioselective bromination and iodination of the nonsteroidal anti-inflammatory drug (NSAID) carprofen were achieved by using bromine and iodine monochloride in glacial acetic acid. The novel halogenated carprofen derivatives were functionalized at the carboxylic group by esterification. The regioselectivity of the halogenation reacti...
Article
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Gastric cancer has remained in the top five cancers for over ten years, both in terms of incidence and mortality due to the shortage of biomarkers for disease follow-up and effective therapies. Aiming to fill this gap, we performed a bioinformatics assessment on our data and two additional GEO microarray profiles, followed by a deep analysis of the...
Article
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Silver doped hydroxyapatite [AgHAp, Ca10−xAg(PO4)6(OH)2], due to its antimicrobial properties, is an advantageous material to be used for various coatings. The AgHAp thin films with xAg = 0.05 and xAg = 0.1 were achieved using the spin-coating method. The resulting samples were examined by X-ray diffraction (XRD), scanning electron microscopy (SEM)...
Article
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Azulene-containing chalcones have been synthesized via Claisen–Schmidt condensation reaction. Their chemical structure has been established by spectroscopic methods where the 1H-NMR spectra suggested that the title chalcones were geometrically pure and configured trans (J = 15 Hz). The influence of functional groups from azulene-containing chalcone...
Article
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In this review, we focus on gut microbiota profiles in infants and adults colonized (CDC) or infected (CDI) with Clostridioides difficile . After a short update on CDI epidemiology and pathology, we present the gut dysbiosis profiles associated with CDI in adults and infants, as well as the role of dysbiosis in C. difficile spores germination and m...
Article
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SARS-CoV-2 vaccines are highly efficient against severe forms of the disease, hospitalization and death. Nevertheless, insufficient protection against several circulating viral variants might suggest waning immunity and the need for an additional vaccine dose. We conducted a longitudinal study on the kinetics and persistence of immune responses in...
Article
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Abstract: Graphite was oxidized to graphene oxide and activated by thionyl chloride, for further covalently linking three hydrazides with potential biological activity. The obtained materials were characterized by scanning electron microscopy with energy dispersive spectroscopy, Fourier-transform infrared and Raman spectroscopies. The presence of v...
Article
Objective: Laryngeal cancer is the second most common malignancy in the head and neck, with Epstein-Barr virus infection as a risk factor. Our aim is to evaluate correlations between the expression of lncRNA H19 and EBV infection in laryngeal cancer and H19 involvement in neoplastic progression through EZH2 association. Materials and methods: 30...
Article
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One major warning emerging during the first worldwide combat against healthcare-associated infections concerns the key role of the surface in the storage and transfer of the virus. Our study is based on the laser coating of surfaces with an inorganic/organic composite mixture of amorphous calcium phosphate–chitosan–tetracycline that is able to figh...
Article
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Angiogenesis is a multi-stage process of new blood vessel development from pre-existing vessels toward an angiogenic stimulus. The process is essential for tissue maintenance and homeostasis during embryonic development and adult life as well as tumor growth. Under normal conditions, angiogenesis is involved in physiological processes, such as woun...
Article
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Liquid biopsy represents an exciting new area in the field of cancer diagnosis and management, offering a less invasive and more convenient approach to obtain a time-point image of the tumor burden and its genomic profile. Samples collected from several body fluids, mostly blood, can be used to gain access to circulating tumor cells and DNA, non-co...
Article
Bacterial infections represent a health issue worldwide. Over the past years, major interest has been given to developing new antibacterial and regenerative materials due to the increasing number of infections with pathogenic strains and the alarming antibiotic resistance. Polymer films and membranes with protective or even anti-infectious activity...
Article
Commercial nanopowders of MgB2 were characterized from the viewpoint of granulometric distribution, structure, microstructure, and pH behavior in water. The powders are very different: a higher amount of the MgB2 phase with a lower tendency for agglomeration determines a higher rate of pH-increase. A higher rate of pH-increase usually produces a st...
Article
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Six compounds of the types [M(dmbg)2]·nH2O ((1) M: Ni, n = 0; (4) M: Cu, n = 1; Hdmbg: N,N′‐dimethylbiguanide) and, respectively, [ML]·nH2O (where (2) M: Ni, L: L¹, n = 0; (3) M: Ni, L: L², n = 0; (5) M: Cu, L: L1, n = 0 and (6) M: Cu, L: L2, n = 3, H2L¹: ligand resulted from the condensation of (1) with ammonia and formaldehyde and H2L²: ligand re...
Article
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Microbial infections associated with skin diseases are frequently investigated since they impact on the progress of pathology and healing. The present work proposes the development of freeze-dried, glutaraldehyde cross-linked, and non-cross-linked biocomposite dressings with a porous structure, which may assist the reepithelization process through...
Article
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Hydroxyapatite Ca10(PO4)6(OH)2 (HAp) is an important bioactive material for bone tissue reconstruction, due to its highly thermodynamic stability at a physiological pH without bio-resorption. In the present study, the Ag:HAp and the corresponding Ag:HAp + D3 thin films (~200 nm) coating were obtained by vacuum deposition method on Ti substrate. The...
Article
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The 17th-19th century wooden and stone churches are an iconic symbol for the Romanian national heritage, raising urgent needs for the development of efficient and ecofriendly restoration and preservation solutions. Nanotechnology has a great but largely unexplored potential in this field, providing new tools and methods to achieve higher consolidat...
Article
The aim of this study was to to evaluate and correlate the expression of some actors of canonical inflammasome pathway with the degree of cervical lesions in cytologic specimens. Methods: The study group included 128 Romanian female patients aged between 16 and 68 years that were referred to Synevo laboratories for performing both a cytological eva...
Article
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Three novel Co(II) complexes of the type [Co(C4H5O2)2L2] (where C4H5O2 is methacrylate anion; L = C3H4N2 (imidazole; HIm) (1), C4H6N2 (2-methylimidazole; 2-MeIm) (2), C5H8N2 (2-ethylimidazole; 2-EtIm) (3)) have been synthesized and characterized by elemental analysis, IR and UV-Vis spectroscopic techniques, thermal analysis and single crystal X-ray...
Article
The lack of complete information on the immune response dynamics to infection with severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) has led to the use of mainly molecular tests such as reverse transcription PCR (RT-PCR) to diagnose Coronavirus 2019 disease (COVID-19). Although remarkable progress has been made in developing effective RT...
Article
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The derivatives 5,7-dimethyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (1) and 7-methyl-5-phenyl[1,3,4]thiadiazolo[3,2-a]pyrimidin-4-ium-2-thiolate (2) were fully characterized by single-crystal X-ray diffraction. Their supramolecular structure is built through both π–π stacking and C=S–π interactions for both compounds. The embedment of t...
Article
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New complexes of type [M(tbg)2]Cl2 [tbg = 1‐(o‐tolyl)biguanide; M = Ni(II), Pd(II), and Pt(II)] were synthesized and characterized to develop new biologically active compounds. The features of the complexes were assigned from microanalytical and thermal data. The NMR, FT‐IR, and UV‐Vis spectra were established by comparison with HtbgCl. All complex...
Article
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The essential oil from inflorescences of S. canadensis L. (Goldenrod) obtained by hidrodistillation was analysed by gas chromatography-mass spectrometry and it was qualitatively and quantitatively tested against Gram-positive and Gram-negative bacteria and fungi. The samples were also subjected to screening for their possible antioxidant activity b...
Article
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Due to their antimicrobial, immunomodulatory, antioxidant, and regenerative activities, culinary herbs have multiple medicinal uses, among which to prevent and treat oral diseases. The whole essential oils (EOs) have multiple advantages over purified components, such as a low probability to select for antimicrobial resistance, synergic effects of d...
Article
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Starting from isoniazid and carboxylic acids as precursors, thirteen new hydrazides and 1,3,4-oxadiazoles of 2-(4-substituted-phenoxymethyl)-benzoic acids were synthesized and characterized by appropriate means. Their biological properties were evaluated in terms of apoptosis, cell cycle blocking, and drug metabolism genes expression on HCT-8 and H...
Article
Background: Gastric cancer (GC) remains an aggressive malignancy with a high rate of mortality, being the third leading cause of cancer-related death. More than one million newly diagnosed cases and 782685 deaths due to GC were reported in 2018. GC is characterized by limited effective treatment options and the lack of consistent biomarkers for th...
Article
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This study aimed to look into the expression of 10 virulence factors (VF) in 100 Escherichia coli strains harvested from the Black Sea Coast. E. coli strains were grown under different conditions, simulating various environmental stressors. The VF production [esculinase, amylase, pore-forming enzymes (hemolysin-spot and CAMP-like hemolysis; lipase;...
Article
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Antimicrobial resistance (AMR) is a significant global threat to both public health and the environment. The emergence and expansion of AMR is sustained by the enormous diversity and mobility of antimicrobial resistance genes (ARGs). Different mechanisms of horizontal gene transfer (HGT), including conjugation, transduction, and transformation, hav...
Presentation
Full-text available
The prevalence of C. albicans isolates in various specimens is presented in figure 2. The highest number of C. albicans isolates (45) occurred in upper respiratory specimens (URTS), followed by urine samples (23), wound secretion (20), blood cultures (05) and stool cultures (03).
Article
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A series of nanofibrous composite mats based on polyurethane urea siloxane (PUUS), hydroxypropyl cellulose (HPC) and β-cyclodextrin (β-CD) was prepared using electrospinning technique. PUUS was synthesized by two steps solution polymerization procedure from polytetramethylene ether glycol (PTMEG), dimethylol propionic acid (DMPA), 4,4′-diphenylmeth...
Article
The role of cancer stem cells in gastrointestinal cancer-associated death has been widely recognized. Gastrointestinal cancer stem cells (GCSCs) are considered to be responsible for tumor initiation, growth, resistance to cytotoxic therapies, recurrence and metastasis due to their unique properties. These properties make the current therapeutic tri...
Article
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The increasing threat of antimicrobial resistance to all currently available therapeutic agents has urged the development of novel antimicrobials. In this context, a series of new benzoylthiourea derivatives substituted with one or more fluorine atoms and with the trifluoromethyl group have been tested, synthesized, and characterized by IR, NMR, CH...
Article
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Silver-zinc oxide nanoparticles (Ag-ZnO NPs) were chemically synthesized by the deposition of Ag NPs on the surface of ZnO NPs using silver nitrate, three types of anionic polyelectrolytes and citric acid as reagents. The Wavelength Dispersive X-ray Fluorescence (WDXRF) spectrometry of Ag-ZnO NPs revealed 0.41-0.69 wt.% Ag, and balance ZnO. The exi...
Article
Bovine hydroxyapatite (BHA) and BHA blended with clinoptilolite (CLIN) and alumina (Al2O3) coatings were synthesized by pulsed laser deposition with a KrF* excimer laser source (λ = 248 nm, τFWHM ≤ 25 ns). Physical-chemical characteristics and the potential use of coatings for preventing bacteria adhesion and biofilms formation were investigated. O...
Article
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In a drug-repurposing-driven approach for speeding up the development of novel antimicrobial agents, this paper presents for the first time in the scientific literature the synthesis, physico-chemical characterization, in silico analysis, antimicrobial activity against bacterial and fungal strains in planktonic and biofilm growth state, as well as...
Article
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In this paper, we aimed to exploit and combine in the same molecule the carbazole and the 1,3,4-oxadiazole pharmacophores, to obtain novel carprofen derivatives, by using two synthesis pathways. For the first route, the following steps have been followed: (i) (RS)-2-(6-chloro-9H-carbazol-2-yl)propanonic acid (carprofen) treatment with methanol, yie...
Article
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We designed new pyrazole derivatives as inhibitors of the cell cycle kinases and developed a simple environmentally sustainable synthesis process. We synthesized the pyrazolyl thiourea derivatives using rapid ultrasound mediated methods and confirmed their structures by NMR and IR spectra. The apoptosis and necrosis inducing effects of the new comp...
Article
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This paper presents the synthesis, physico‐chemical and biological properties of four new coordination compounds with mixed ligands: acrylate ion (acr) and benzimidazole/benzimidazole derivatives with the general formula [Co(L)2(acr)2]·nH2O [(1) L: benzimidazole (HBzIm), n: 0.5; (2) L: 2‐methylbenzimidazole (2‐MeBzIm), n: 0.5; (3) L: 5‐methylbenzim...
Article
Three novel fluorescent mesoporous silica composites were obtained through the covalent immobilization of 7-amino-4-(trifluoromethyl)coumarin, 6-amino-chromen-2-one and 7-amino-4-methyl-3-coumarinylacetic acid, respectively, inside the channels of mesoporous silica SBA-15. Presence of fluorescent moieties was assessed by elemental analysis, thermal...
Article
Full-text available
Gastric cancer (GC) remains an important cause of cancer death worldwide with a high mortality rate due to the fact that the majority of GC cases are diagnosed at an advanced stage when the prognosis is poor and the treatment options are limited. Unfortunately, the existing circulating biomarkers for GC diagnosis and prognosis display low sensitivi...
Article
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Synthesis of biomimetic materials for implants and prostheses is a hot topic in nanobiotechnology strategies. Today the major approach of orthopaedic implants in hard tissue engineering is represented by titanium implants. A comparative study of hybrid thin coatings deposition was performed by spin coating and matrix-assisted pulsed laser evaporati...
Article
Single ZnO crystallites assembled into porous hierarchical structures have been prepared by topotactic thermal decomposition of in situ obtained zinc oxalate precursors, whose synthesis involves a redox reaction between 1,2-ethanediol and nitrate ion. For the first time it was demonstrated that post-synthesis protocols of the precursors (e.g. ultra...
Article
Full-text available
Methotrexate (MTX) is a folic acid analog with anti-proliferative (anti-neoplastic, cytotoxic), immunosuppressive and anti-inflammatory properties, which has been used in the treatment of various cutaneous disorders, such as psoriasis, keratoacanthoma, pityriasis rubra pilaris, atopic dermatitis, mycosis fungoides, bullous skin diseases, systemic s...
Article
In this work, we present a simple, two-stage method for the preparation of new and efficient antimicrobial hybrid systems, based on isoniazid and pyrazine-2-carbohydrazide tuberculostatic agents covalently linked to graphene oxide. In the first step, the carboxyl groups of graphene oxide are activated by transforming them into the corresponding aci...
Presentation
The A. fruticosa essential oil has been proved to have antimicrobial activity by acting on the cell membrane level by disrupting the efflux pump action. The stock solutions manifested antipathogenic effect by inhibiting the ability of bacteria to inert and cell substrates adhesion. Therefore, the stock solution of the A. fruticosa essential oil sho...
Poster
Solidagocanadensis, a native species of North America,is an invasive-agessive species in Romania and Europe.The purpose of this paper was to give it a real usage by investigated the antimicrobial and antiphatogenic effects of S. canadensis essential oil thereby harnessing the therapeutic potential.
Article
Full-text available
Simple and lithium-doped biological-origin hydroxyapatite layers were synthesized by Pulsed Laser Deposition technique on medical grade Ti substrates. Cytotoxic effects of lithium addition and the biocompatibility of obtained coatings were assessed using three cell lines of human origin (new initiated dermal fibroblasts, immortalized keratinocytes...
Article
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Melanoma is the most severe type of skin cancer and its incidence has increased in the last decades. In the United States, it is the 6th most common cancer in both men and women. Prognosis for patients with melanoma depends on the stage of the disease at the time of diagnosis and it can be influenced by the immunologic response. Melanoma has been h...