Constanza Macaya Ramos

• Universidad Técnica Federico Santa María

Halotolerant Halomonas spp. SpR1 and SpR8 are potential plant growth-promoting bacteria (PGPB) isolated from Salicornia rhizosphere in a Chilean Altiplano hydrothermal lagoon. We report draft genomes of Halomonas sp. SpR1 (5.17Mb) and Halomonas sp. SpR8 (4.47 Mb). Both represent potentially novel independent species closely related to Halomonas bol...

Soil plays an essential role in life. It provides the habitat for diverse animals, plants, protists and microorganisms. Soil is the physical place where diverse biogeochemical cycles occur. Phenomena such as soil erosion, nutrient depletion, low organic matter content, changes in pH, pollution and high variability in rainfall distribution generate...

Extremophiles are organisms capable of adjust, survive or thrive in hostile habitats that were previously thought to be adverse or lethal for life. Chile gathers a wide range of extreme environments: salars, geothermal springs, and geysers located at Altiplano and Atacama Desert, salars and cold mountains in Central Chile, and ice fields, cold lake...

Edu+ cells found at early- and mid-elongation stages. EdU positive cells divided by the area of the germband analyzed (SAZ or trunk) were plotted showing differences between SAZ and trunk at both stages. Error bars represent the standard error of the mean; ****P<0.00001. (TIF)

Late germband elongation in the absence of cell proliferation. A-B Representative pictures of control (left embryo) and treated (right embryo) mid-elongated germbands after 2 h exposition to 0.5% DMSO (sham controls; A) and aphidicolin+hydroxyurea (B). Scale bar: 100 μm. C Embryo length measurement after aphidicolin+hydroxyurea treatment. The lengt...

EdU+ cells counting at mid elongation stages. Table summary of the number of EdU positive cells and the areas where they were found within the SAZ and trunk, showing the statistical analysis and an example of the method using the software ImageJ. Scale bar: 100 μm. (TIF)

Determination of SAZ length by Tc-caudal gene expression. Representative pictures of embryos at (A) early elongation + 60 minutes and (B) mid elongation + 60 minutes expressing Tc-caudal gene within the entire SAZ region (black brackets). A Based on this analysis, we determined that 60 minutes after dissection, SAZ covers 37.1% of the length of the...

Effect of the absence of proliferation on cell viability and EdU incorporation. A-D’ Cell death assay using propidium iodide (PI). Representative pictures of 2 h control (left half; A, A’), 3 h control (left half; C, C’), 2 h (right half; B, B’) and 3 h (right half; D, D’) aphidicolin+hydroxyurea treated germband halves. A-D GFP (green) and PI (red...

EdU+ cells counting at early elongation stages. Table summary of the number of EdU positive cells and the areas where they were found within the SAZ and trunk, showing the statistical analysis and an example of the method using the software ImageJ. Scale bar: 100 μm. (TIF)

Mid elongation stage. A-B Tribolium embryo at mid elongation stage. A Representative picture of an embryo when it is selected for dissection at mid elongation stage, when the first abdominal segments start to appear rapidly according to Nakamoto et al. [21], approximately when the 6th– 7th Tc-engrailed stripe arise (B), The black arrow points the f...

Segment length shortening after cell division inhibition. Segment length of control and APH+HU treated embryos for incubation periods of 1, 2 and 3 hours (hour post treatment). Number of analyzed embryos: 1 hpt n = 5 for both, control and treated embryos At 2 hpt n = 5 for control embryos and n = 6 for treated embryos. At 3 hpt, n = 5 for both, con...

Most arthropods generate their posterior bodies by adding segments periodically, as the embryo grows, from a posteriorly located region called the segment addition zone. This mode of segmentation is shared with vertebrates and relies on oscillatory mechanisms, where the temporal periodicity of a clock is translated into repetitive spatial patterns....

The development of the red flour beetle Tribolium castaneum is more representative of arthropods than the evolutionarily derived fly, Drosophila melanogaster. Thus, Tribolium is becoming an emerging organism model for studying the evolution of the mechanisms that control embryonic development in arthropods. In this regard, diverse genetic and molec...