Constanza-Javiera Aguilera-Gonzalez

Constanza-Javiera Aguilera-Gonzalez
University of Concepción · Facultad de Ciencias Veterinarias

Doctor of Veterinary Medicine

About

29
Publications
1,613
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
32
Citations

Publications

Publications (29)
Article
This work aims to evaluate the efficiency of detecting embryonic DNA in extracellular vesicles (EVs) secreted by in vitro produced bovine embryos. It is hypothesized that embryonic DNA is released within EVs, and thus, the isolation of these vesicles would improve the use of spent medium as a noninvasive method for embryo sexing and genotyping. The...
Article
Full-text available
Over the last few years, several commercial FSH products have been developed for cattle superovulation (SOV) purposes in Multiple Ovulation and Embryo Transfer (MOET) programs. The SOV response is highly variable among individuals and remains one of the main limiting factors in obtaining a profitable number of transferable embryos. In this study, f...
Article
Full-text available
As células-tronco mesenquimais (CTM) são utilizadas na terapia celular, isolando-se de diferentes tecidos, incluindo a pele. Os fibroblastos dérmicos têm mostrado características de potência semelhantes às CTMs como expressão de marcadores de superfície e diferenciação para outros tipos de linhagens de origem mesodérmica, principalmente sob a influ...
Article
Full-text available
During early development, embryos secrete extracellular vesicles (EVs) that participate in embryo-maternal communication. Among other molecules, EVs carry microRNAs (miRNAs) that interfere with gene expression in target cells; miRNAs participate in embryo-maternal communication. Embryo selection based on secreted miRNAs may have an impact on bovine...
Article
Human embryos generated in vitro have a high incidence of chromosomal abnormalities that negatively affect pregnancy rate. Embryos generated in vitro secrete extracellular vesicles (EVs) into the culture medium that could be used potentially as indicators of embryo competence. This research aimed to evaluate the concentration and size of EVs and th...
Article
Abstract The kodkod (Leopardus guigna) is a small felid endemic of Chile and is considered a vulnerable species. Domestic cat oocytes have been successfully used as recipient cytoplast to reprogram somatic cells from different felids by interspecific somatic cell nuclear transfer (iSCNT). The developmental competence of felid embryos generated by i...
Article
Full-text available
Adipose derived mesenchymal stem cells (AMSCs) have been isolated from domestic and wild cats. For wild cats, the isolation of AMSCs has been reported in the black-footed cats (Felis nigripes) and guigna (Leopardus guigna). Stromal vascular fraction (SVF) isolated from cougar adipose tissue have been used to restore elbow functionality in the couga...
Article
The objective of this study was to evaluate, in the domestic cat, the effect of ovarian stimulation with eCG prior to oocyte in vitro maturation (priming) on in vitro and in vivo development after in vitro fertilization (IVF). For this purpose, oocyte donors were either 1) treated with a single dose of 200 IU eCG four days before oocyte recovery (e...
Article
Noninvasive methods are the clue to increase the efficiency of invitro-derived embryo selection without decreasing their competence. Embryos selection based on their morphology is the most used method but only 40% of selected embryos are able to implant and develop correctly. In humans, pre-implantation genetic diagnosis increases the efficiency of...
Article
Reprogramming of terminally differentiated cells to higher plasticity levels can be achieved with small molecules. This can be of value for somatic cell nucleus transfer, deriving multi and pluripotent cells and conservation purposes. Recently, induced mesenchymal stem cells were derived from differentiated human and mouse cells by using small mole...
Article
Extracellular vesicles (EVs), including exosomes and microvesicles, are secreted by different cell types and participate in cellular communication by carrying molecules as microRNAs (miRNAs) that can interfere with gene expression of target cells. Extracellular vesicles have become relevant as a mechanism of embryo-maternal communication. The aim o...
Article
Limited information have been reported regarding to the role of the zona pellucida during embryo development in the domestic cat. In the present research we compared invitro and invivo development of cat embryos generated with versus without the zona pellucida. Embryos were produced by IVF and cultured up to blastocyst stage accordingly to experime...
Article
Full-text available
Adipose derived mesenchymal stem cells (AMSCs) have been isolated from domestic and wild cats. For wild cats, the isolation of AMSCs has been reported in the black-footed cats (Felis nigripes) and guigna (Leopardus guigna). Stromal vascular fraction (SVF) isolated from cougar adipose tissue have been used to restore elbow functionality in the couga...
Article
Full-text available
The domestic cat is a valuable model for the development of assisted reproductive techniques that might be used in the conservation of endangered felids. However, the efficiency of the in vitro embryo production in the domestic cat remains low compared to other species. In the bovine and equine, the zona pellucida removal enhance the developmental...
Article
Full-text available
Adipose tissue derived mesenchymal stem cells (AMSCs) had been isolated and used for cell therapy in domestic cats. For wild cats, the isolation of AMSCs has only been reported in the black-footed cat (Felis nigripes). AMSCs obtained from wild cats may be useful to treat injuries of endangered cat species that remain in captivity or arrive at wildl...
Article
The kodkod is considered a vulnerable species by the International Union for Conservation of Nature. Phylogenetically, the kodkod is classified in the Leopardus genus, which has only 36 chromosome pairs compared with the domestic cat, which has 38. The proposed hypothesis was that domestic cat oocytes are capable of reprogramming somatic cells from...
Poster
Full-text available
El uso de células madre mesenquimales (MSCs) aisladas de varios tejidos representan una oportunidad para ser empleadas en terapia celular en animales domésticos y silvestres. Aunque en gatos domésticos se ha descrito ampliamente su aislamiento y caracterización desde diferentes tejidos, en felinos salvajes existen pocos registros de aislamiento de...
Poster
Full-text available
Obtaining mesenchymal stem cells (MSC) might be troublesome for some species, particularly the feline. Further, in patients with complex pathologies or trauma like wild cats, when biopsy sampling is an issue, it can be even more complicated. The objective of this study was to produce h-PDGF-B by stable transfection of human SiHa cells for future us...
Conference Paper
Full-text available
En la actualidad, se describe un aumento en los trabajos de investigación relacionados con el desarrollo de técnicas de reproducción asistida en el gato doméstico. Esto con el propósito de implementar posteriormente estas técnicas en la conservación de felinos silvestres amenazados (Pope, 2014). Sin embargo, los sistemas de producción in vitro de e...
Poster
Full-text available
Cell therapy is an option currently used for treatment of several pathologies in many species. Cat adipose mesenchymal stem cells (MSCs) have been used successfully in certain therapies in domestic cats. However, availability and easiness of access to these cells in feline patients has been an issue. Mouse embryonic fibroblast as well as terminally...
Poster
Full-text available
Las células madre mesenquimales (MSC) felinas se han obtenido de diversos tejidos, siendo el tejido adiposo el más empleado para fines terapéuticos en gatos domésticos. Estas células presentan capacidad de diferenciación hacia linajes mesodérmicos y expresión de marcadores de superficie similares a los reportados para las AMSC humanas (CD44+, CD90+...

Network

Cited By