Concepcion Jimenez-Lopez

Concepcion Jimenez-Lopez
University of Granada | UGR · Department of Microbiology

Bs. D. Physics, Ms.D. Physics, Ms.D. Microbiology, PhD Physics, Full Professor in Microbiology

About

87
Publications
15,516
Reads
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2,406
Citations
Citations since 2017
38 Research Items
1309 Citations
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2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
Introduction
Concepcion Jimenez-Lopez currently works at the Department of Microbiology, University of Granada. Concepcion does research in Microbiology, Biotechnology and Geology. Their current project is 'Bioproduction of metallic nanoparticles and their technological applications'.
Additional affiliations
October 2003 - present
University of Granada
Position
  • Professor (Associate)
October 2003 - present
University of Granada
Position
  • Professor (Associate)
Description
  • Microbiology Industrial Microbiology Biomineralization
October 2000 - July 2002
University of Georgia
Position
  • Fullbright Posdoc
Education
September 2007 - July 2009
University of Granada
Field of study
  • Microbiology
September 1996 - July 1998
University of Granada
Field of study
  • Physics
September 1990 - June 1995
University of Granada
Field of study
  • Physics

Publications

Publications (87)
Article
It has never been demonstrated whether magnetite synthesized through the heat-dependent decomposition of carbonate precursors retains the chemical and structural features of the carbonates. In this study, synthetic (Ca,Mg,Fe)CO3 was thermally decomposed by heating from 25 to 700 °C under 1 atm CO2, and by in situ exposure under vacuum to the electr...
Article
Full-text available
Biomimetic magnetic nanoparticles mediated by magnetosome proteins (BMNPs) are potential innovative tools for cancer therapy since, besides being multifunctional platforms, they can be manipulated by an external gradient magnetic field (GMF) and/or an alternating magnetic field (AMF), mediating targeting and hyperthermia, respectively. We evaluated...
Article
Full-text available
ABSTRACT: Immobilization of enzymes has been extensively required in a wide variety of industrial applications as a way to ensure functionality and the potential of enzyme recycling after use. In particular, enzyme immobilization on magnetic nanoparticles (MNPs) could offer reusability by means of magnetic recovery and concentration, along with inc...
Article
Biominerals are important archives of the presence of life and environmental processes in the geological record. However, ascribing a clear biogenic nature to minerals with nanometer-sized dimensions has proven challenging. Identifying hallmark features of biologically controlled mineralization is particularly important for the case of magnetite cr...
Article
Full-text available
Citation: Donini, M.; Pettinella, F.; Zanella, G.; Gaglio, S.C.; Laudanna, C.; Jimenez-Carretero, M.; Jimenez-Lopez, C.; Perduca, M.; Dusi, S. Effects of Magnetic Nanoparticles on the Functional Activity of Human Monocytes and Dendritic Cells. Int. J. Mol. Sci. 2023, 24, 1358. https:// (M.P.) † These authors contributed equally to this work. ‡ Thes...
Article
Full-text available
Among the strategies employed to overcome the development of multidrug-resistant bacteria, directed chemotherapy combined with local therapies (e.g., magnetic hyperthermia) has gained great interest. A nano-assembly coupling the antimicrobial peptide AS-48 to biomimetic magnetic nanoparticles (AS-48-BMNPs) was demonstrated to have potent bactericid...
Article
The importance of egg natural defences to prevent bacterial contamination and their relation with hen age in extended production cycles were evaluated. Egg-white from eggs of different hen age groups (up 100-weeks-old) and lines (Hy-Line white and brown) were inoculated with Gram-positive Staphylococcus aureus or Gram-negative Salmonella Typhimuriu...
Poster
Full-text available
Aim: The rationale behind this work lies in an optimization of the nanoformulation DOXO- BMNPs, already demonstrated to be more efficient against tumor cells, both in vitro and in vivo, than systemic traditional therapies. By embedding DOXO-BMNPs into PLGA, which is further functionalized with the cell- penetrating TAT peptide, the resulting nanoas...
Article
The design of new strategies to increase the effectiveness of the antibacterial treatments is a main goal in public health. So, the aim of the study was to achieve a local antibacterial directed therapy as novel alternative allowing both, the delivery of the drug at the target, while minimizing undesirable side effects, thus anticipating an enhance...
Article
Full-text available
The synergy between directed chemotherapy and thermal therapy (both magnetic hyper-thermia and photothermia) mediated by a nanoassembly composed of functionalized biomimetic magnetic nanoparticles (BMNPs) with the chemotherapeutic drug doxorubicin (DOXO) covered by the polymer poly(lactic-co-glycolic acid) (PLGA), decorated with TAT peptide (here r...
Article
Full-text available
Magnetotactic bacteria (MTB) biomineralize intracellular magnetite (Fe3O4) crystals surrounded by a magnetosome membrane (MM). The MM contains membrane-specific proteins that control Fe3O4 mineralization in MTB. Previous studies have demonstrated that Mms13 is a critical protein within the MM. Mms13 can be isolated from the MM fraction of Magnetosp...
Article
Full-text available
MamC-mediated biomimetic magnetic nanoparticles (BMNPs) have emerged as one of the most promising nanomaterials due to their magnetic features (superparamagnetic character and large magnetic moment per particle), their novel surface properties determined by MamC, their biocompatibility and their ability as magnetic hyperthermia agents. However, the...
Article
Full-text available
Citation: Vurro, F.; Jabalera, Y.; Mannucci, S.; Glorani, G.; Sola-Leyva, A.; Gerosa, M.; Romeo, A.; Romanelli, M.G.; Malatesta, M.; Calderan, L.; et al. Improving the Cellular Uptake of
Article
Full-text available
The design of novel nanomaterials that can be used as multifunctional platforms allowing the combination of therapies is gaining increased interest. Moreover, if this nanomaterial is intended for a targeted drug delivery, the use of several guidance methods to increase guidance efficiency is also crucial. Magnetic nanoparticles (MNPs) allow this co...
Article
Recent studies have shown the potential of magnetic hyperthermia in cancer treatments. However, the underlying mechanisms involved have not been yet fully described. In particular, the cell death related to magnetic hyperthermia observed in cultures incubated with low concentration of magnetic nanoparticles and under a low intensity alternating mag...
Article
Hydrogels present model systems for biopolymer matrices in biological structural materials, as their fabric and physicochemical properties can be tailored to mimic characteristics of polymer matrices in biological hard tissues. However, hydrogels comprise synthetic compounds and lack attributes of native biopolymers, in contrast to extracellular po...
Article
Full-text available
Current chemotherapy for colorectal cancer (CRC) includes the use of oxaliplatin (Oxa), a first-line cytotoxic drug which, in combination with irinotecan/5-fluorouracil or biologic agents, increases the survival rate of patients. However, the administration of this drug induces side effects that limit its application in patients, making it necessar...
Article
Biomimetic magnetite nanoparticles (BMNPs) synthesized in the presence of MamC, a magnetosome-associated protein from Magnetoccus marinus MC-1, have gained interest for biomedical applications because of their unique magnetic properties. However, their behavior in biological systems, like their interaction with proteins, still has to be evaluated p...
Article
An increasing concern for natural resources preservation and environmental safety is the removal of heavy metals from contaminated water. It is essential to develop simple procedures that use ecofriendly materials with high removal capacities. In this context, we have synthesized a new hybrid material in which eggshell membranes (ESMs) act as nucle...
Article
Mineralized structures generated under biological control are hierarchical composites that consist of biopolymer matrices and minerals. The biopolymer matrix in biological material is developed as membranes or as a network of fibrils, which has structural as well as functional roles for the composite hard tissue. Microorganisms are surrounded by mi...
Article
Full-text available
In the field of Nanomedicine, there is an increasing demand for new inorganic nanophosphors with low cytotoxicity and efficient loading-release ability of drugs for applications in bioimaging and drug delivery. This work assesses the potentiality of matured Eu-doped citrate-coated carbonated apatite nanoparticles to be used as theranostic platforms...
Article
Magnetite nanoparticles (MNPs) are being used in a number of nanotechnological applications, especially biomedical, both in diagnosis and therapeutics such as hyperthermia agents and as drug nanocarriers for targeted chemotherapy. However, the development of efficient methodologies to produce novel MNPs with the specific requirements needed for bio...
Article
Full-text available
Choline kinase α1 (ChoKα1) has become an excellent antitumor target. Among all the inhibitors synthetized, the new compound Ff35 shows an excellent capacity to inhibit ChoKα1 activity. However, soluble Ff35 is also capable of inhibiting choline uptake, making the inhibitor not selective for ChoKα1. In this study, we designed a new protocol with the...
Article
Full-text available
Conventional chemotherapy against colorectal cancer (CRC), the third most common cancer in the world, includes oxaliplatin (Oxa) which induces serious unwanted side effects that limit the efficiency of treatment. Therefore, alternative therapeutic approaches are urgently required. In this work, biomimetic magnetic nanoparticles (BMNPs) mediated by...
Article
Recently, liposomes have been explored as a potential solution to improve the biocompatibility and the colloidal stability of magnetic nanoparticles. Protocols have been developed for producing magnetoliposomes of magnetite nanoparticles obtained inorganically (MNPs). However, the biomimetic synthesis of magnetite using heterologous proteins from m...
Article
Full-text available
The role of magnetosome associated proteins on the in vitro synthesis of magnetite nanoparticles has gained interest, both to obtain a better understanding of the magnetosome biomineralization process and to be able to produce novel magnetosome-like biomimetic nanoparticles. Up to now, only one recombinant protein has been used at the time to in vi...
Article
Full-text available
In this work we report on the synthesis and characterization of magnetic nanoparticles of two distinct origins, one inorganic (MNPs) and the other biomimetic (BMNPs), the latter based on a process of bacterial synthesis. Each of these two kinds of particles has its own advantages when used separately with biomedical purposes. Thus, BMNPs present an...
Article
MamC, an integral protein of the magnetosome membrane, has recently been proposed as a strong candidate to produce biomimetic (magnetosome-like) magnetite nanoparticles that could be used as an alternative to magnetosomes in different applications such as nanocarriers. The secondary structure of the protein contains two helical transmembrane domain...
Article
Magnetite nanoparticles in the magnetosomes formed by magnetotactic bacteria present unique magnetic properties that make them the ideal nanoparticle with potential use in several biotechnological applications. These magnetoliposomes are organelles formed by crystals of magnetite (Fe 3 O 4) or greigite (Fe 3 S 4) surrounded by a lipid bilayer. Howe...
Article
Full-text available
Biomineralization is the process of mineral formation by organisms and involves the uptake of ions from the environment in order to produce minerals, with the process generally being mediated by proteins. Most proteins that are involved in mineral interactions are predicted to contain disordered regions containing large numbers of negatively charge...
Article
MamC from Magnetococcus marinus MC-1 has been shown to control the size of magnetite crystals in in vitro experiments, thereby demonstrating its potential as a candidate protein for the production of magnetite nanoparticles possibly useful in medical and other applications. However, the importance of the structure and aggregation state of the prote...
Article
The suggestion in 1996 that the Martian meteorite ALH84001 could contain proof of possible biologic activity in the past have generated a huge controversy that last until today. One of the most discussed evidence is the presence of magnetite crystals that resemble those produced by a particular group of bacteria, the so called magnetotactic bacteri...
Article
Magnetotactic bacteria are a diverse group of prokaryotes that share the unique ability of biomineralizing magnetosomes, which are intracellular, membrane-bounded crystals of either magnetite (Fe3O4) or greigite (Fe3S4). Magnetosome biomineralization is mediated by a number of specific proteins, many of which are localized in the magnetosome membra...
Article
Full-text available
The biomineralization of magnetotactic bacterial magnetite nanoparticles is a topic of intense research due to the particles' narrow size distribution and magnetic properties. Incorporation of foreign metal ions into the crystal matrix of magnetotactic bacterial magnetite has been previously examined by a number of investigators. In this study, cel...
Article
Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures i...
Article
Full-text available
Magnetotactic bacteria are a diverse group of prokaryotes that biomineralize intracellular magnetosomes, composed of magnetic (Fe3O4) crystals each enveloped by a lipid bilayer membrane that contains proteins not found in other parts of the cell. Although partial roles of some of these magnetosome proteins have been determined, the roles of most ha...
Article
Full-text available
Biological macromolecules are utilized in low-temperature synthetic methods to exert precise control over nanoparticle nucleation and placement. They enable low-temperature formation of a variety of functional nanostructured materials with properties often not achieved via conventional synthetic techniques. Here we report on the in situ visualizati...
Article
Magnetite is a common iron oxide produced both inorganically and biogenically. Biologically-induced magnetite is often originated, under appropriate conditions, as a result of the Fe3+ reduction by dissimilatory iron reducing bacteria, which are usually found in anoxic environments or at the oxic-anoxic interface. Such a Fe3+ bioreduction occurs up...
Article
Stone consolidation treatments that use bacterial biomineralization are mainly based on two strategies: (1) the inoculation of a bacterial culture with proven carbonatogenic ability and/or (2) the application of a culture medium capable of activating those bacteria able to induce the formation of calcium carbonate, from amongst the bacterial commun...
Article
An easy, low-cost coprecipitation method to inorganically produce magnetite nanoparticles from solutions, in free-drift experiments, under anoxic conditions, at 25 °C and 1atm pressure is here presented. By using this method, pure magnetite is obtained as the final solid, which shows the typical magnetic properties and thermal stability behavior of...
Chapter
Calcium carbonate precipitation is a common phenomenon in nature and has been observed to be mediated by a number of microorganisms (for a review, see Castanier et al. 2000; Wright and Oren 2005). Bacterially induced carbonate mineralization is important in a wide range of processes including atmospheric CO2 budgeting (Braissant et al. 2002; Ehrlic...
Article
Full-text available
In this study, we investigated under laboratory conditions the bacterial communities inhabiting quarry and decayed ornamental carbonate stones before and after the application of a Myxococcus xanthus-inoculated culture medium used for consolidation of the stones. The dynamics of the community structure and the prevalence of the inoculated bacterium...
Article
Full-text available
Over the years, nanometer-sized magnetite (Fe3O4) crystals have been recovered from many modern and ancient environments including sediments and soils and even meteorites. In some cases these crystals have been used as ``magnetofossils'' for evidence of the past presence of specific microbes. Magnetite nanocrystals can be formed by a number of diff...
Article
Incorporation of foreign metal into the crystal matrix of the magnetotactic bacterial magnetite has been attempted worldwide. Recently, presence of small amounts of cobalt and manganese in magnetosome magnetite crystals in cultured and uncultured magnetotactic bacteria, respectively, was reported. Magnetization of the uncultured cells and their mag...
Article
Shewanella oneidensis is a dissimilatory iron reducing bacterium capable of inducing the extracellular precipitation of magnetite. This precipitation requires a combination of passive and active mechanisms. Precipitation occurs as a consequence of active production of Fe2+(aq) when bacteria utilize ferrihydrite as a terminal electron acceptor, and...
Article
Full-text available
The objective of the present research was to determine at what level the shell mineralization of A. cygnea, a shallow freshwater bivalve, is influenced by external physical-chemical factors, mainly concerning the habit and microstructure of the CaCO3 crystals. So, a detailed description of the inner shell layers of A. cygnea was carried out by scan...
Article
A set of free-drift experiments was undertaken to synthesize carbonates of mixed cation content (Fe, Ca, Mg) from solution at 25 and 70 °C to better understand the relationship between the mineralogy and composition of these phases and the solutions from which they precipitate. Metastable solid solutions formed at 25 °C which are not predicted from...
Article
In situ Atomic Force Microscopy, AFM, experiments have been carried out using calcite cleavage surfaces in contact with solutions of MgSO4, MgCl2, Na2SO4 and NaCl in order to attempt to understand the role of Mg2+ during calcite dissolution. Although previous work has indicated that magnesium inhibits calcite dissolution, quantitative AFM analyses...
Article
Pure magnetites are obtained from ankerite thermal decomposition. Chemical purity is typical of bacterial origin magnetites. Chemical purity of magnetites produced by thermal decomposition of ankerites containing different cations was analyzed.
Article
Full-text available
Número de publicación: 2 289 950 Número de solicitud: 200602030 Método y producto para la protección y consolidación de materiales de construcción y ornamentales que activa la microbiota natural presente en los materiales de construcción, de manera que induzca la formación de carbonato cálcico. Como consecuencia de su aplicación se consigue la acti...
Article
A new method is described to protect and to increase the consolidation of new pieces of stone that will replace damaged ones in restoration procedures, in order to make the new piece more resistant to deterioration. Culture media were applied to quarry porous limestone whose natural microbial community was not eliminated. The culture media activate...
Article
Full-text available
Biological carbonate mineralization induced by both microorganisms and higher phyla organisms is very important in many different natural processes. The organisms precipitate calcium carbonate to form very sophisticated biomaterials that they used for many different functions. Organisms control calcium carbonate precipitation using specific organic...
Article
It has been claimed that chemically pure magnetites (Fe3O4) can be obtained from thermal decomposition of (Fe, Mg, Ca)CO3 (Golden et al., 2004). Such an observation is critical, since it opens the possibility of an inorganic way of formation of the magnetites found on Martian meteorite ALH84001. Such a chemical purity is one of the parameters used,...
Article
Several mechanisms have been proposed to explain the interactions between proteins and mineral surfaces formed during biomineralization. To investigate the effect of the surface charge of proteins on calcium carbonate precipitation, a group of globular proteins with similar sizes and molecular weights but with different isoelectric points (iep) has...
Article
Full-text available
Myxococcus xanthus is a Gram negative, non-pathogenic, common soil bacterium that belongs to the δ- subdivision of the Proteobacteria. According to the data, it is demonstrated that, depending on the chemis- try of the culture media, M. xanthus is able to induce the formation of phosphates (struvite, schertelite, newberyite), carbonates (calcite, M...