Colette Breuil

Colette Breuil
University of British Columbia - Vancouver | UBC · Faculty of Forestry

About

329
Publications
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Publications

Publications (329)
Article
Full-text available
13 As an environmentally friendly alternative to chemical treatment, this research aimed to establish 14 whether dielectric heating at high frequency of infested lodgepole pine (Pinus contorta) boards and 15 logs, obtained from the mountain pine beetle devastated forests of British Columbia, can result in wood 16 free of living fungi, nematodes and...
Article
As an environmentally friendly alternative to chemical treatment, this research aimed to establish whether dielectric heating at high frequency of infested lodgepole pine (Pinus contorta) boards and logs, obtained from the mountain pine beetle devastated forests of British Columbia, can result in wood free of living fungi, nematodes and insects. Th...
Article
To successfully colonize and eventually kill pine trees, Grosmannia clavigera (Gs), the main fungal pathogen associated with the mountain-pine beetle (Dendroctonus ponderosae), has developed multiple mechanisms to overcome host tree chemical defenses, of which terpenoids are a major component. In addition to a monoterpene efflux system mediated by...
Article
Chitinases have been implicated in the defence of conifers against insects and pathogens. cDNA for six chitinases were cloned from interior spruce (Picea glauca x engelmannii) and four from lodgepole pine (Pinus contorta). The cloned interior spruce chitinases were annotated class I PgeChia1-1 and PgeChia1-2, class II PgeChia2-1, class IV PgeChia4-...
Article
Full-text available
Studies on beetle/tree fungal symbionts typically characterize the ecological and geographic distributions of the fungal populations. There is limited understanding of the genome-wide evolutionary processes that act within and between species as such fungi adapt to different environments, leading to physiological differences and reproductive isolat...
Article
Full-text available
Background Ophiostoma piceae is a wood-staining fungus that grows in the sapwood of conifer logs and lumber. We sequenced its genome and analyzed its transcriptomes under a range of growth conditions. A comparison with the genome and transcriptomes of the mountain pine beetle-associated pathogen Grosmannia clavigera highlights differences between a...
Book
Full-text available
Because of global trade, and environmental and climate changes, phytophagous insects and insect-vectored fungi have the potential to undergo rapid population expansion and threaten the ecological and economic sustainability of forests. Native bark beetles and their fungal associates, which evolve within trees, are among the most damaging forest pes...
Article
Full-text available
Background The mountain pine beetle (MPB, Dendroctonus ponderosae) epidemic has affected lodgepole pine (Pinus contorta) across an area of more than 18 million hectares of pine forests in western Canada, and is a threat to the boreal jack pine (Pinus banksiana) forest. Defence of pines against MPB and associated fungal pathogens, as well as other p...
Article
Grosmannia clavigera is a bark beetle-vectored pine pathogen in the mountain pine beetle epidemic in western North America. Grosmannia clavigera colonizes pines despite the trees' massive oleoresin terpenoid defences. We are using a functional genomics approach to identify G. clavigera's mechanisms of adaptation to pine defences. We annotated the A...
Conference Paper
The mountain pine beetle (MPB) is a native bark beetle of western North America that attacks pine tree species, particularly lodgepole pine. It is closely associated with the ophiostomatoid ascomycetes Grosmannia clavigera, Leptographium longiclavatum, Ophiostoma montium and Ceratocystiopsis sp.1, with which it is symbiotically associated. To devel...
Article
Grosmannia clavigera is a fungal associate of the mountain pine beetle (Dendroctonus ponderosae) and a pathogen of lodgepole pine (Pinus contorta) that must overcome terpenoid oleoresin and phenolic defenses of host trees. G. clavigera responds to monoterpene influx with complementary mechanisms that include export and the use of these compounds as...
Article
Full-text available
The mountain pine beetle (MPB) is a native bark beetle of western North America that attacks pine tree species, particularly lodgepole pine. It is closely associated with the ophiostomatoid ascomycetes Grosmannia clavigera, Leptographium longiclavatum, Ophiostoma montium, and Ceratocystiopsis sp.1, with which it is symbiotically associated. To deve...
Data
Identified genes and FunCat assignments.xls (excel spreadsheet).
Article
Full-text available
The highly aggressive pathogenic fungus Ophiostoma novo-ulmi continues to be a serious threat to the American elm (Ulmus americana) in North America. Extensive studies have been conducted in North America to understand the mechanisms of virulence of this introduced pathogen and its evolving population structure, with a view to identifying potential...
Article
Grosmannia clavigera is a fungal pathogen of pine forests in western North America and a symbiotic associate of two sister bark beetles: Dendroctonus ponderosae and D. jeffreyi. This fungus and its beetle associate D. ponderosae are expanding in large epidemics in western North America. Using the fungal genome sequence and gene annotations, we asse...
Article
Different soil extract media and other media were assayed for a comparative evaluation of both total and lipolytic populations of bacteria taken from soil and water samples during winter. By incubating at a low temperature, psychrophilic microorganisms were counted and isolated. Gram-negative bacteria, especially Pseudomonas species, were the most...
Article
Bacillus subtilis C186 was evaluated as a potential biological control agent for sapstain and mould growth on unseasoned lumber. The strain produces antibiotics that are fungistatic to many pine-inhabiting fungi. Although pine blocks precolonized with C186 and subsequently inoculated with staining fungi were generally less discoloured than blocks i...
Article
Full-text available
A sensitive immunological tool has been developed to detect the sapstaining fungus Ophiostoma piceae 3871, which plagues the wood industry. Monoclonal antibodies (1F3(1), 4G3(14), 4G2(4), and 2B6(24)) produced against cell wall protein extracts of this fungus were specific. Specificity was estimated by enzyme linked immunosorbent assay, western blo...
Article
Full-text available
Sap-staining fungi, in particular Ophiostoma piceae, cause significant economic problems for Canada's lumber industry. For O. piceae and a few other staining fungi, the dark pigment is melanin. The choice of carbon and nitrogen sources added as supplements to a basic liquid mineral medium affected the colour and characteristics of the pigment produ...
Article
Fungi excrete a variety of extracellular enzymes capable of breaking down complex nutrient sources into products that can be directly metabolized by the fungi. An extracellular proteinase and lipase produced by the sap-staining fungus, Ophiostoma piceae, have been purified and the specificities of these enzymes for a variety of substrates have been...
Article
Full-text available
The Canadian forest products industry suffers considerable losses due to discoloration caused by sapstain fungi. Although studied for a number of years, the identity, biology, and ecology of these fungi are still only partly understood. To determine which fungi caused stain problems, a detailed survey was conducted at seven selected sawmills across...
Article
Analysis of the potential genetic variation in quaking aspen (Populus tremuloides Michx.) wood extractives was performed by sampling nine natural clones at breast height. Significant clonal differences were found not only in the levels of total acetone extractives but also in the levels of sterols–triterpenes, steryl esters–waxes, and triglycerides...
Article
Full-text available
We assessed the pathogenicity of Leptographium longiclavatum sp. nov., a recently reported fungal associate of the mountain pine beetle, Dendroctonus ponderosae (Hopkins, 1902). In September 2003, 30 lodgepole pines (Pinus contorta Dougl. ex Loud. var latifolia Engelm. ex S. Wats) between 98 and 130 years old were inoculated with L. longiclavatum,...
Article
Full-text available
An enzyme-linked immunosorbent assay was developed for the quantitative detection of the sap-staining fungus Ophiostoma sp. C28 in artificially inoculated wood. The fungus can be detected in wood samples as small as 1 mg dry weight and at concentrations much lower than those at which staining becomes visible.
Article
Full-text available
Ophiostoma clavigerum, carried by Dendroctonus ponderosae and Dendroctonus jeffreyi, has morphological characteristics that are similar to other Ophiostoma and Leptographium species. The partial β-tubulin gene of 45 strains belonging to seven species was amplified by PCR and digested by the restriction enzyme HinfI. The specific restriction fragmen...
Article
An enzyme-linked immunosorbent assay (ELISA) was used to detect a sap-staining fungus, Ophiostoma piceae, and a biological-control agent, Gliocladium roseum, grown in liquid culture and in wood. A polyclonal serum prepared against whole cell fragments from broken mycelia of O. piceae detected O. piceae in liquid culture at 0.25 μg dry weight/mL; ho...
Article
Full-text available
The sap-staining ascomycete Ophiostoma piceae strain 387N used ammonium, but not nitrate, as an inorganic nitrogen source. Organic nitrogen sources assimilated included bovine serum albumin, collagen, acid-hydrolyzed casein, urea, and various amino acids. Sucrose, glucose, maltose, raffinose, and soluble starch were suitable carbon sources. The opt...
Article
Polyclonal antibodies were raised against proteinase K and were used to immunolocalize the major extracellular proteinase of the sap-staining fungus Ophiostoma piceae (Münch) H. and P. Sydow. Immunodot blotting showed that the IgG antibodies recognized both enzymes but reacted more strongly with proteinase K than with the O. piceae proteinase. Immu...
Article
Full-text available
In western North America, the current outbreak of the mountain pine beetle (MPB) and its microbial associates has destroyed wide areas of lodgepole pine forest, including more than 16 million hectares in British Columbia. Grosmannia clavigera (Gc), a critical component of the outbreak, is a symbiont of the MPB and a pathogen of pine trees. To bette...
Article
When lodgepole pines (Pinus contorta Douglas ex Louden var. latifolia Engelm. ex S. Watson) that are killed by the mountain pine beetle (Dendroctonus ponderosae) and its fungal associates are not harvested, fungal decay can affect wood and fibre properties. Ophiostomatoids stain sapwood but do not affect the structural properties of wood. In contra...
Article
Full-text available
The research was aimed to establish whether dielectric heating at radio frequencies (RF)—an alternative method to ozone-depleting methyl bromide fumigation and to convective heating—could bring green lodgepole pine boards infested with pinewood nematodes (PWN) to pasteurization requirements. Artificially infected specimens were treated using a labo...
Data
Table S1. Excel file containing the supplementary table with all unigene locations, their coordinates on the G. clavigera genome, the ESTs that mapped to each UL, relevant annotations, and expression analysis results.
Article
Full-text available
Grosmannia clavigera is a bark beetle-vectored fungal pathogen of pines that causes wood discoloration and may kill trees by disrupting nutrient and water transport. Trees respond to attacks from beetles and associated fungi by releasing terpenoid and phenolic defense compounds. It is unclear which genes are important for G. clavigera's ability to...
Article
The aim of this study was to develop DNA probes that could identify the major fungal species associated with mountain pine beetles (MPB). The beetles are closely associated with fungal species that include ophiostomatoid fungi that can be difficult to differentiate morphologically. The most frequently isolated associates are the pine pathogens Gros...
Article
Full-text available
Grosmannia clavigera is a fungal pathogen associated with the mountain pine beetle (Dendroctonus ponderosae) which is devastating large areas of western Canada's conifer forests. This fungus also produces a dark melanin pigment that discolors pine sapwood. We have generated the draft genome of G. clavigera. However, functional characterization of g...
Article
Ceratocystiopsis minuta (Siemaszko) H.P. Upadhyay & W.B. Kendr., originally isolated in Poland, is the type species of genus Ceratocystiopsis H.P. Upadhyay & W.B. Kendr. Species in this genus are characterized by dark perithecia with short conical beaks, usually with convergent ostiolar hyphae and dark ascocarps, and by falcate or lunate ascospores...
Article
The largest forest pest epidemic in Canadian history caused by the mountain pine beetle (MPB) and its fungal associates has killed over 15 million hectares of forest. Sixty simple sequence repeat regions were identified from Grosmannia clavigera, an MPB associated fungus. Eight loci genotyped in 53 isolates from two populations in British Columbia,...
Data
NCBI accession numbers for the trace data used in this study.
Data
Full-text available
Supplementary sections 1: additional explanation for the 454 read filtering and alignments of the 454 pre- and post- filtered Forge assemblies relative to the manually finished GCgb1 sequence. Supplementary sections 2: additional explanation and supporting figures for the filtering and trimming of Illumina PE read data. Supplementary sections 3: su...
Article
Full-text available
Sequencing-by-synthesis technologies can reduce the cost of generating de novo genome assemblies. We report a method for assembling draft genome sequences of eukaryotic organisms that integrates sequence information from different sources, and demonstrate its effectiveness by assembling an approximately 32.5 Mb draft genome sequence for the forest...
Article
Full-text available
We tested the effect of leaching on the concentration of western red cedar (WRC; Thuja plicata Donn ex D. Don) heartwood extractives that are known to exhibit antimicrobial activity and correlated this with fungal growth and decay. We assessed the extractive tolerance of the following fungal species: Acanthophysium lividocaeruleum, Coniophora putea...
Article
Full-text available
Most 'ambrosia' fungi are members of a heterogeneous group of ophiostomatoids that includes the anamorph genera Ambrosiella, Raffaelea and Dryadomyces. The taxonomy of these fungi based on morphological features has been complicated by these features being poorly descriptive and having evolved convergently. In this work we report maximum parsimony...
Article
Full-text available
Grosmannia clavigera is the most pathogenic blue-staining fungal associate of the mountain pine beetle (Dendroctonus ponderosae). In contrast to its importance as a primary invader of lodgepole pine (Pinus contorta) sapwood, intraspecific variability in pathogenicity of G. clavigera on lodgepole pine, the predominant host of mountain pine beetles i...
Article
Characterization of the virulence of bark beetle-vectored fungi is important for assessing potential impacts of beetle outbreaks. Massive inoculation of trees with a cork borer appears to give the most accurate estimate of fungal virulence, but cork borer inoculation is time and labor intensive. In October 2003, 18 Pinus contorta var. latifolia wer...
Article
Phellinus sulphurascens Pilát causes laminated root rot of coniferous species in both western North America (WNA) and Asia. Accurate somatic incompatibility tests for mapping population structures have been difficult to conduct for P. sulphurascens because no single, unambiguous criterion has allowed differentiation of homokaryotic and heterokaryot...
Article
Full-text available
ABSTRACT Wood sapstain, a cosmetic defect that results in significant economical loss to forest-products industries, is caused by mycelial melanization of the wood-colonizing ophiostomatoid fungi. To improve our understanding of how melanin biosynthesis is regulated in the cosmopolitan sapstaining fungus, Ophiostoma piceae, we used insertional muta...
Article
ABSTRACT The sapstaining fungal pathogen Ophiostoma clavigerum is associated with the mountain pine beetle (Dendroctonus ponderosae), which is currently the most destructive forest pest in North America. The genetic diversity of O. clavigerum populations collected from five sites in Canada and two sites in the United States was estimated with ampli...
Article
Full-text available
Western red cedar (Thuja plicata Donn) is valued for its natural durability conferred by fungicidal extractive chemicals. However, weathered surfaces of T. plicata products are susceptible to black stain caused by fungi such as Aureobasidium pullulans. The effect of weathering on extractive concentrations at the wood surface was characterized and c...
Article
Full-text available
Variable wood chip density and extent of decay can significantly affect pulp yield and quality. Rapid methods are needed to quantify and eventually control these variables. Near infrared (NIR) spectroscopy is a rapid technique that may be suitable for measuring these variables. In the present research, NIR spectroscopy was used to develop partial l...
Article
To collect more information about the organisms causing sap-stain, a thorough survey was conducted in Koreas sawmills that process logs and boards of Japanese red pine and Korean pine. Sap-staining fungi were isolated and identified using morphological and molecular methods. The 482 isolates obtained were distributed into at least nine species of o...
Article
Full-text available
Western red cedar (Thuja plicata Donn) is a naturally durable softwood species native to British Columbia in Canada, and Washington, Oregon and California in the USA. Untreated T. plicata products are usually durable because of the presence of extractives with anti-microbial activity. However, there are extractive tolerant fungi that can attack T....