Clyde Goodheart

• Celigenex, Inc.

DNA is the molecule responsible for storing and processing genetic information today. In Earth's primeval environmental conditions, RNA was probably more suited for this function, due to its capability to act also as a catalytic enzyme. Some proteins are stable and reliable molecules even in extreme conditions, and under certain circumstances, prot...

The purpose of this study was to evaluate the ability of fibronectin to augment the regenerative effects of a bovine-derived xenograft in human periodontal defects. Using a parallel arm, randomized double-blind design, 24 patients with an intrabony defect or a Class II furcation defect were randomly assigned to either the experimental group (xenogr...

Fibronectins are a family of glycoproteins with modular functional domains. They mediate cell-cell and cell-matrix interactions which are important in embryogenesis, wound healing, metastasis and other processes. We present data on the influence of fibronectin on wound implantation of a murine mammary carcinoma line, TA3Ha. Fibronectin used in thes...

The standard sex-linked recessive lethal test using "Basc" virgin females and treated Canton S males was used to test potential mutagenicity of ingested butylated hydroxyanisole (BHA) on mature spermatozoa. Ethyl methanesulfonate (EMS) was employed as a positive control and at lower concentrations a roughly linear dose response curve resulted. Flie...

Sixty strains of herpes simplex viruses from various sites of isolation were compared with one another by plaque appearance, by neutralization tests, by pock sizes on the chorioallantoic membrane of the chick embryo, and by the stabilities of their infectivity and of their thymidine kinase activities. On the basis of each of these tests (except the...

Mouse cytomegalovirus replicated in rabbit kidney cultures, a cell system of nonrodent origin. However, the sensitivity of these cultures, and the yields of virus therefrom, were lower than those of mouse cultures. Although a cytopathic effect developed in rabbit kidney cultures inoculated with sufficient amounts of the virus, such cultures were un...

Summary Nucleic acid hybridization techniques are highly sensitive methods for determining the presence of herpes simplex type 2 genomes. Measurement of the rate of reassociation between labeled, purified viral DNA and DNA extracted from the tumor cells may be necessary to achieve adequate sensitivity if only a portion of the viral genome is presen...

Oncogenic herpesviruses, like many other viruses, can be concentrated effectively from large volumes of culture fluids by precipitation with methanol with good recovery of infectivity.

Oncogenic herpesviruses, like many other viruses, can be concentrated effectively from large volumes of culture fluids by precipitation with methanol with good recovery of infectivity.

Equine herpesviruses with a deoxyribonucleic acid density of 1.716 to 1.717 g/cm(3) were compared with one another by the plaque-reduction test and by the rate of development of cytopathic effect as indicated by plaque size in rabbit kidney cultures. Of the 19 isolates studied, the 9 which had already been tentatively labeled equine abortion viruse...

Attachment and growth of mammalian cells plated at low cell density require optimum conditions for the cells to form colonies. Reliability, reproducibility, and validity of the plating efficiency test for evaluating cell culture sera were determined by measuring the plating efficiency of 37 lots of fetal bovine serum obtained from 8 suppliers (5 lo...

In this brief review, the author discusses molecular hybridization methods that are available for use with herpes viruses. It now seems technically possible to make a survey of populations of women with cervical carcinoma to determine the indidence of viral genetic material in the tumors. Additional data on how many genomes are contained by the mal...

Attachment and growth of mammalian cells plated at low cell density require optimum conditions for the cells to form colonies. Reliability, reproducibility, and validity of the plating efficiency test for evaluating cell culture sera were determined by measuring the plating efficiency of 37 lots of fetal bovine serum obtained from 8 suppliers (5 lo...

MCMV was recovered from abdominal lymph nodes and spleens of chronically infected C3H and Balb/c mice by prolonged co-cultivation with mouse embryonic fibroblasts. Intranuclear inclusions were not found histologically in spleens from infected mice nor were herpes-type viruses seen in the electron microscope. Virus was isolated from mice for as long...

To obtain information on inhibition of adenovirus transformation by AAV, cultures of hamster embryo cells were inoculated simultaneously with constant amounts of simian adenoviruses SA7 or SV11, or with human adenovirus type 12 and with varying multiplicities of AAV-1. Induction of transformed cell foci by SV11 or Ad12 was inhibited by more than 80...

Forty isolates of herpes simplex virus were compared by means of cross-neutralization curves. The 11 oral isolates were serotype 1, and all 29 genital/anal isolates were serotype 2. The cytopathic effects of the two serotypes were consistently different. Passage of strains of type 1 and type 2 in mice and in rabbits yielded two variants, although t...

Four equine herpesviruses (equine abortion virus, equine herpesvirus types 2 and 3, and equine cytomegalovirus) were compared. The equine abortion virus did not cross-neutralize with any of the other viruses, but the other three did show varying degrees of cross-neutralization among themselves. Equine abortion virus grew more quickly in tissue cult...

Human leukocyte cultures were infected with adenovirus type 12 that had been labeled with tritiated thymidine and purified. In the conditions of the experiment, the virus attaches to the cells but does not replicate. By autoradiography, three patterns of labeling were observed. Some interphase cells had labeled nuclei, some had label in both nucleu...

When cultured human fetal fi-broblasts were infected by human cytomegalo-virus, virus-induced morphologic changes consisting of cytopathology, intracytoplasmic and intranuclear inclusion bodies developed during a 72-hour period. Thus, in this system, virus-induced cell changes followed closely upon viral multiplication. Inhibitors of DNA synthesis,...

About 5 to 20% of the 32P of EMC virus adsorbed to mouse embryo cells can be recovered in the progeny virus. By use of a recently described density mutant of EMC virus, evidence has been obtained indicating that the small recovery of radioactivity in the progeny is not due to transfer but is due to “carried-over” parental virus that did not take pa...

Clonally derived adenovirus types 12 and 18 induced tumors in hamsters. Specific complement fixing antigens were demonstrated in the tumors, and the tumored hamsters developed specific complement fixing antibodies to both tumor and T antigens. The histologic characteristics of the tumors resembled those previously described for tumors induced by th...

1. Biologically active labeled human growth hormone, prepared by limited acetylation with tritiated acetic anhydride, was injected intravenously into rats and guinea pigs. 2. In both rats and guinea pigs the radioactivity was distributed primarily to skeletal muscle, liver, intestine, kidney, bone, fat, and urine. 3. Autoradiographic studies indica...

Equilibrium centrifugation Of a suspension of encephalomyocarditis virus (EMC) in CsCl reveals infectious particles of various densities. The majority of particles (>99.7%) forms a Gaussian distribution with a modal density of 1.333–1.335 g/ml. The remaining particles form one or more minor bands at greater densities than the majority. Evidence, in...

Autoradiographic studies were made of the synthesis and migration of DNA fofnd in the intranuclear and intracytoplasmic inclusions of human cytomegalovirus-infected cells. The DNA of the intranuclear inclusion, presumably viral DNA, forms de novo, beginning aboft 24 hofrs after infection and continuing until' at least 96 hofrs after infection. This...

Human cytomegalovirus grown in cultures of human fetal skin-muscle cells was examined by high resolution electron microscopy using the phosphotungstate method for negative contrast. The virus particle shows evidence of icosahedral shape and consists of a capsid (960 A diameter) composed of 162 elongated hollow capsomeres (135 A × 95 A) and an inner...

An assay method for human cytomegalovirus is described that is based on counts of infected embryonic human skin and muscle fibroblastic cells on coverslips inoculated with 0.1 ml of diluted virus suspension. Counts made 48 hours after inoculation, when the preparations are fixed and stained, include all initially infected cells. Counts of infected...

The evolution of the intracytoplasmic and intranuclear lesions produced by a human cytomegalovirus in human embryo cells is described and the lesions characterized cytochemically. Twenty-four hours after infection basophilic, lipid-containing, PAS-positive bodies surrounded by a halo containing RNA appear in a juxtanuclear position in the cytoplasm...

A formula based on results of Robertson and Hughes has been developed ; for calculating the average radiation doserate distribution within a cell ; containing intranuclear trit ium. The formula has been used to calculate a curve ; giving average dose rate as a function of distance from the center of a nucleus ; of 2-micron radius. The dose rate to...

Static elastance of the lungs-chest of seven curarized dogs averaged 15.2 ± 4.9 cm H 2 O/l. Elastance decreased with time probably because of stress relaxation. The expiratory volume flow rate for five different initial inflations was recorded as a function of time. Nondimensionalized plots were nonlinear, especially with the larger initial inflati...