Cleofe Palocci

Cleofe Palocci
Sapienza University of Rome | la sapienza · Department of Chemistry

PhD

About

79
Publications
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Publications

Publications (79)
Article
Gene delivery is the basis for developing gene therapies that, in the future, may be able to cure virtually any disease, including viral infections. The use of short interfering RNAs (siRNAs) targeting viral replication is a novel strategy for treating HIV-1 infection. In this study, we prepared chitosan particles containing siRNA tat/rev via ionot...
Article
Full-text available
Botrytis cinerea, responsible for grey mold diseases, is a pathogen with a broad host range, affecting many important agricultural crops, in pre and post harvesting of fruits and vegetables. Commercial fungicides used to control this pathogen are often subjected to photolysis, volatilization, degradation, leaching, and runoff during application. In...
Article
Hydrogels based on short peptide molecules are interesting biomaterials with wide present and prospective use in biotechnologies. A well-known possible drawback of these materials can be their limited mechanical performance. In order to overcome this problem, we prepared Fmoc-Phe3self-assembling peptides by a biocatalytic approach, and we reinforce...
Article
Full-text available
18β-glycyrrhetinic acid (GA) is a pentacyclic triterpene with promising hepatoprotective and anti-Hepatocellular carcinoma effects. GA low water solubility however reduces its biodistribution and bioavailability, limiting its applications in biomedicine. In this work we used core-shell NPs made of PolyD-L-lactide-co-glycolide (PLGA) coated with chi...
Article
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Recent advances in nanotechnology techniques enable the production of polymeric nanoparticles with specific morphologies and dimensions and, by tailoring their surfaces, one can manipulate their characteristics to suit specific applications. In this work we report an innovative approach for the immobilization of a commercial lipase from Candida rug...
Article
Full-text available
Olive oil production generates high amounts of liquid and solid wastes. For a long time, such complex matrices were considered only as an environmental issue, due to their polluting properties. On the other hand, olive mill wastes (OMWs) exert a positive effect on plant growth when applied to soil due to the high content of organic matter and miner...
Article
The goal of supporting and directing tissue regeneration requires the design of new, advanced materials, with features like biocompatibility, biodegradability and adequate mechanical properties. Our work was focused on developing a new injectable biomimetic composite material, based on a peptidic hydrogel and calcium phosphates with the aim of mimi...
Article
Full-text available
The objective of the present work was to synthesize biopolymeric nanoparticles (NPs) entrapping the resistance-inductor methyl jasmonate (MeJA) to be employed as a novel and alternative strategy in integrated pest management. NPs were prepared by using a continuous flow microfluidic reactor that allows to precisely control some features that are cr...
Article
Enzyme immobilization on nanocarriers is nowadays considered a useful tool for improving activity and maintaining biocatalysts stability while facilitating their recovery and reuse. In this work we prepared Au and Ag based nanoparticles (AuNPs or AgNPs) stabilized with two different ligands, the organometallic dinuclear complex trans,trans-[dithiod...
Article
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In recent years, great attention has been focused on rapid, selective, and environmentally friendly extraction methods to recover pigments and antioxidants from microalgae. Among these, supercritical fluid extraction (SFE) represents one of the most important alternatives to traditional extraction methods carried out with the use of organic solvent...
Article
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Polymeric nanoparticle-based carriers are promising agents to deliver drugs to cells. Vitis vinifera phenolic compounds are known for their antifungal activity against Candida albicans. The aim of the present study was to investigate the antifungal activity of pterostilbene or crude extracts from non-fermented grape pomace, entrapped in poly(lactic...
Article
Doxorubicin (DOX) is commonly used to treat several tumor types, but its severe side effects, primarily cardiotoxicity, represent a major limitation for its use in clinical settings. In this study we developed and characterized biodegradable and stable poly(D,L-lactic-co-glycolic) acid (PLGA) submicrocarriers employing an osmosis-based patented met...
Article
The topical treatment for oral mucosal diseases is often based on products optimized for dermatologic applications; consequently, a lower therapeutic effect may be present. 18-β-Glycyrrhetic Acid (GA) is extracted from Glycirrhiza glabra. The first aim of this study was to test the cytotoxicity of GA on PE/CA-PJ15 cells. The second aim was to propo...
Article
Peptide-based hydrogels have been widely used for both tissue engineering approaches and therapeutic drug delivery mainly thanks to their tissue-like water content and tunable physicochemical properties. In particular, the modulation of the hydrogel chemical structure influences hydrogel properties. The most common approach for tuning hydrogel prop...
Article
Full-text available
Key message: PLGA NPs' cell uptake involves different endocytic pathways. Clathrin-independent endocytosis is the main internalization route. The cell wall plays a more prominent role than the plasma membrane in NPs' size selection. In the last years, many studies on absorption and cell uptake of nanoparticles by plants have been conducted, but th...
Article
The immobilization procedure is one of the key factors on the electron transfer based biosensors performance. It must assure both the enzyme retention activity and an efficient communication between the redox center of the enzyme and the electrode surface. In this regard, hydrogels are attractive materials due to their three-dimensional hydrophilic...
Article
The applications of peptide-based materials are currently expanding, especially in the biomedical field. The biocompatibility and biodegradability of peptide materials, as well as their ability to assemble into ordered secondary structures, are indeed ideal for biotechnological applications. However, their full potential will be exploited once nove...
Article
The chemical treatment called “chemotherapy of plants” consists in the in vivo administration of substances able to interfere with viral replication. The current availability of synthetic molecules with a high chemotherapeutic index, i.e. with a high ratio between the maximum concentration tolerated and the minimum effective, together with the poss...
Article
Polyhydroxybutyrate (PHB) is a bacterial storage polyester, which is produced directly in the activated sludge process. In the present study, PHB was tested as a scaffold material for stabilization of nanosized zero-valent iron (nZVI). The morphology of the resulting composite was assessed using transmission electron microscopy (TEM) and scanning e...
Article
Polyhydroxybutyrate (PHB) is a bacterial storage polyester, which is produced directly in the activated sludge process. In the present study, PHB was tested as a scaffold material for stabilization of nanosized zero-valent iron (nZVI). The morphology of the resulting composite was assessed using transmission electron microscopy (TEM) and scanning e...
Chapter
The production of polyhydroxyalkanoate (PHA) via microbial mixed cultures (MMC) is attracting considerable research interest due to the possibility to easily use a large variety of low-cost feedstock (i.e., agro-industrial wastes and wastewaters), which, in turn, makes it feasible to obtain a wide range of PHA copolymers with different thermal and...
Article
Full-text available
Groundwater is an extremely important resource that may, however, contain a variety of toxic and bioaccumulative contaminants. Traditional "Pump and Treat" technologies for treating contaminated groundwater are no longer time- or cost-effective; therefore, new technologies are needed. In this work, we synthesized core-shell materials of micrometric...
Article
Full-text available
Currently, many microbial infections have the potential to become lethal owing to the development of antimicrobial resistance by means of different mechanisms and mainly on the basis of the fact that many drugs are unable to reach therapeutic levels in the target sites. This requires the use of high doses and frequent administrations, causing adver...
Article
The synthesis and optimization of peptide-based hydrogel materials have gained growing interest in the last years, thanks to their properties, that make them appealing for diverse biotechnological applications, with a particular focus in the field of biomedicine. The self-assembling abilities of low molecular weight peptides make them ideal for des...
Article
Full-text available
Recently, scientific and technological interest in the synthesis of novel peptide-based hydrogel materials have grown dramatically. Applications of such materials mostly concern the biomedical field with examples covering sectors such as drug delivery, tissue engineering, and production of scaffolds for cell growth, thanks to their biocompatibility...
Article
The goal of this study was to develop an optimized drug delivery carrier for oral mucosa applications able to release in situ bioactive molecules by using biopolymeric materials. Among them chitosan and polylactic (co-glycolic) acid have gained considerable attention as biocompatible carriers able to improve the delivery of active agents. The formu...
Article
Polymer-based nanoparticles (NPs) can be efficiently used for the delivery of bioactive molecules for both in vitro and in vivo applications affording high drug loading and controlled release profiles. Within this framework polylactic-co-glycolic acid (PLGA) NPs with a diameter of 290 ± 41 nm have been fabricated and loaded with dexamethasone (DXM)...
Chapter
Grape cultivation dates back to approximately 6000-8000 years ago. Nowadays it is still one of the major crops produced worldwide, mostly for wine production. Accordingly, grape pomace, the solid remain of the wine making process, is produced in large quantities. The disposal of such waste material is an issue of great ecologic and economic importa...
Article
Full-text available
Poly(lactic-co-glycolic) acid (PLGA)-based NPs are currently considered among the most promising drug carriers, nevertheless their use in plants has never been investigated. In this work, for the first time, we demonstrated the ability of PLGA NPs to cross the plant cell wall and membrane of Vitis vinifera cell cultures and grapevine-pathogenic fun...
Article
Full-text available
Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p...
Article
Using a novel and versatile capillary microfluidic flow-focusing device we fabricated monodisperse drug-loaded nanoparticles from biodegradable polymers. A model amphiphilic drug (dexamethasone) was incorporated within the biodegradable matrix of the particles. The influence of flow rate ratio, polymer concentration, and microreactor-focusing chann...
Conference Paper
Objective: Recently, polymeric nanoparticles (NPs) have attracted interest as delivery vehicles for bioactive molecules, with the potential to overcome poor drug solubility or cell permeability. Moreover, the synthesis of porous biomaterials for tissue engineering applications can benefit from coupling scaffolds with nanoparticles releasing growt...
Article
Full-text available
We have recently employed l-amino acids in the lipase-catalyzed biofabrication of a class of self-assembling Fmoc-peptides that form 3-dimensional nanofiber scaffolds. Here we report that using d-amino acids, the homochiral self-assembling peptide Fmoc-d-Phe3 (Fmoc-F*F*F*) also forms a 3-dimensional nanofiber scaffold that is substantially distingu...
Article
In this work, we investigated the possibility to extract oleanolic acid through supercritical fluid extraction from waste materials such as grape pomace, which can represent a yet unexploited source of highly added-value products. Preliminary supercritical fluid extractions were performed on dried grape skin samples and compared with solid–liquid e...
Article
A recently patented one-step methodology was used for the formulation of chitosan (CS) coated polylactic-co-glycolic acid (PLGA) nanoparticles containing dexamethasone (DXM) as a model drug. SEM investigations showed that nanoparticles (NPs) were spherical in shape with smooth surface. CS coating switched NPs ζ-potential from negative to positive,...
Article
Peptidic hydrogels are an interesting class of materials, with potential applications in tissue engineering and controlled drug delivery. Fmoc–Phe3 self-assembling peptide was synthesized in an aqueous phase by using a lipase from Pseudomonas fluorescens. The bioconversion afforded a self-supporting hydrogel in only 10 minutes, a feature that seems...
Article
Poly(phenylacetylene) (PPA) and poly(phenylacetylene-co-acrylic acid) (P(PA-co-AA)), nanoparticles bioconjugated with dexamethasone (DXM) during the synthesis, named PPA@DXM and P(PA-co-AA)@DXM, were prepared by a modified surfactant free emulsion method. The loading was studied as a function of different functionality grades of the copolymer and d...
Article
Chitosan-modified polylactic-co-glycolic acid (PLGA) nanoparticles with average diameter of 200 nm in PBS buffer solution have been investigated by means of dielectric relaxation spectroscopy measurements in the frequency range (1 MHz-2 GHz) where interfacial polarizations occur. PLGA-based nanoparticles offer remarkable advantages in different bio...
Article
Peptide applications are growing significantly in the field of biomedicine (e.g.immunology, cell signaling, etc.). A recent projection valued that the global peptidetherapeutics market would be worth nearly $2 billion in 2010. Such a significant andrapid development is due to the major clinical value that the specificity of peptides hasbeen acknowl...
Article
The adsorption of Candida rugosa lipase (CRL) on different types of polymeric nanoparticles, both synthetic and derived from natural sources, was studied. Comparing to non nanostructured carriers, polymeric nanoparticles were able to induce a stabilization of the adsorbed CRL to different denaturing agents such as temperature, medium pH or organic...
Article
Full-text available
Interfacing proteins with nanostructured materials offers the possibility to obtain novel bioconjugates for many applications. We report herein the ability of nanostructured poly-DL-lactic acid (PDLLA) based carriers to enhance enzymatic activity and stability. PDLLA was processed using an innovative patented methodology that permitted to obtain sp...
Chapter
The role of nanomaterials and nanotechnology in life sciences is yet to be fully understood, as it has resulted in the constantly developing field of nanobiotechnology. In fact, nanomaterials can interact with biomacromolecules as well as with living systems and these interactions can be used to develop new materials and technologies which are fore...
Article
Full-text available
Self-assembling gelling peptides are increasingly being investigated as defined biomatrices for biomedical applications. Using an enzymatic reaction to convert a precursor into a hydrogelator one can control or modulate functions and responses of a hydrogel, depending on its preparation conditions. This work details the self-assembly, under physiol...
Article
Polymers derived from monomers with a variety of functionalities provide materials with a vast range of properties and applications. Worldwide research has recently developed a wide number of methods suitable for the preparation of polymeric materials of nanometric dimensions, in view of the fact that, at the nanoscale level, new and unexpected pro...
Article
Candida rugosa lipase (CRL) was used to catalyze the regioselective acylation of methyl 6-O-trityl β-d-glucopyranoside in supercritical CO2 (SCCO2), using vinyl acetate as the acyl donor and obtained final conversion values up to 91.4%. The effect of pressure, temperature, enzyme quantity and sugar/ester molar ratio on the catalytic efficiency and...
Article
Full-text available
Polysaccharides and other cationic polymers have recently been used in pharmaceutical research and industry for their properties to control the release of antibiotics, DNA, proteins, peptide drugs or vaccines, and they have also been extensively studied as non-viral DNA carriers for gene delivery and therapy. Among them, chitosan is the most used s...
Article
Nanostructured polystyrene (PS) and polymethylmethacrylate (PMMA) were used as carriers for the preparation of bioconjugates with lipolytic enzymes, such as Candida rugosa lipase (CRL) and Pseudomonas cepacia lipase (PCL). Simple addition of the lipase solution to the polymeric nanoparticles under protein-friendly conditions (pH 7.6) led to the for...
Article
Full-text available
Highly porous, hydrophilic porous matrices were fabricated by using a high internal phase supercritical-CO2 (scCO2) emulsion templating technique. The novel aspect of the work resides in the combination of a natural biopolymer (dextran) as the building component of the matrices and of an environmentally benign solvent (supercritical-CO2) as the por...
Article
The objective of the present work was to contribute to the understanding of the physiological role of latex lipolytic activity in Euphorbia characias. To this end, the acid and basic lipolytic activity of E. characias latex, as well as the substrate specificity on various triacylglycerols, were measured during the plant's vegetative and reproductiv...
Article
Candida rugosa lipase crude preparations (CRL) catalyse the regioselective acylation of methyl 6-O-trytil beta-d-glucopyranoside in organic solvents, using vinyl acetate as acyl donor. The ratio of the two products formed, namely methyl 2-O acetyl 6-O-trytil beta-d-glucopyranoside and methyl 3-O acetyl 6-O-trytil beta-d-glucopyranoside was found to...
Article
Polysaccharides and other cationic polymers have been recently used in pharmaceutical research and industry for their properties to control the release of antibiotics, DNA, proteins, peptide drugs or vaccines. They have been also extensively studied as non viral DNA carriers for gene delivery and therapy. Chitosan is one of the most used since it c...
Article
Pseudomonas cepacea lipase (PCL) was used to catalyse the transesterification reaction between 1-phenylethanol and vinyl acetate in supercritical carbon dioxide (SCCO2). The enzyme, which is very selective towards one of the isomers of 1-phenylethanol, was very stable in the supercritical medium and showed higher reaction rates. The effect of SCCO2...
Article
Polyphenylacetylene (PPA) was studied and characterized as a new matrix for Candida rugosa lipase (CRL) immobilization, establishing π interactions with the enzyme. Performances of the new biocatalytic system were compared to different hydrophobic matrices (cross-linked polyvinyl alcohol esterified with lauric acid—CL-PVA-C12, commercial polypropyl...
Article
We generated monoclonal antibodies (MoAbs) BF11 and VNH9 against Candida rugosa lipase (CRL), one of the most widely used lipolytic enzymes. The antibodies, both of the IgG1 isotype recognize specie specific and distinct antigenic determinants shared by different CRL isoforms present both in the native and in the denaturated form of the enzyme. The...
Article
To investigate interfacial activation phenomenon on lipolytic enzymes we carried out kinetic studies employing the monolayer technique. The use of a substrate in monofilm state allows the monitoring, during the biocatalysed reactions, of several physico-chemical parameters and permits the modification of the ‘quality of interface’. The substrates u...
Article
The activity and substrate specificity of latex lipases from Euphorbia species (E. characias, E. wulfenii, E. pinea, E. myrsinites and E. dendroides) were investigated. High lipolytic activity was found only in E. characias and for the first time in E. wulfenii latex. For both species the lipolytic activity on various triglycerides, and under diffe...
Article
(+/-)-2-(6-Methoxy-2-naphthyl)propionic acid methyl ester (methyl ester of Naproxen), the precursor of therapeutically important nonsteroidal anti-inflammatory drugs (NSAIDs) was enantioselectively hydrolysed using as biocatalyst Candida rugosa lipase. In research aimed at studying the structure-activity relationship (SAR), NMR spectroscopy methods...
Article
Immobilized lipase from Mucor miehei (RML) catalyzed the regioselective acylation of the C-2 side-chain of the C-alkyl resorcin[4]arene tetra-alcohol 1 in the 1,2-alternate form in organic solvents using vinyl acetate as acylating reagent. The influence of reaction parameters and solvent choice were also studied. Docking simulations allowed the det...
Article
We studied an enantioselective lipase-catalysed transesterification reaction for the production of the statin lactone moiety. It is well known that the chiral cyclic structure of statins is very important for their pharmaceutical activity in the prevention and treatment of coronary artery diseases.We focused our attention on 3R,5R-6-(ethyl-2-phenyl...
Article
Within a research project aimed at probing the substrate specificity and the enantioselectivity of Candida rugosa lipase (CRL), computer modeling studies of the interactions between CRL and methyl (+/-)-2-(3-benzoylphenyl)propionate (Ketoprofen methyl ester) have been carried out in order to identify which amino acids are essential to the enzyme/su...
Article
Within a research project aimed at probing the substrate specificity and the enantioselectivity of Candida rugosa lipase (CRL), computer modeling studies of the interactions between CRL and methyl (±)-2-(3-benzoylphenyl)propionate (Ketoprofen methyl ester) have been carried out in order to identify which amino acids are essential to the enzyme/subs...