Clémence Guiraut

Clémence Guiraut
Université de Montréal | UdeM · Department of Pediatrics

Master of Science in Immunology

About

22
Publications
2,175
Reads
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177
Citations
Citations since 2017
9 Research Items
172 Citations
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Introduction
My research focuses on stroke and infectious/inflammatory aggressions occurring perinatally and leading to severe handicaps, such as cerebral palsy, learning disabilities and behavioural disorders. Through preclinical approaches, I have have shown that prenatal exposure to bacterial components induces cerebral injuries, through cytokines release, and predisposes to circulatory defects occurring around birth.
Additional affiliations
February 2015 - present
McGill University
Position
  • Research Assistant
May 2012 - April 2015
Université de Sherbrooke
Position
  • Master's Student
April 2011 - April 2012
Université de Sherbrooke
Position
  • BSc student

Publications

Publications (22)
Article
Background: Most very premature newborns (< 32 weeks of gestation) receive parenteral nutrition (PN) that is inherently contaminated with peroxides. Oxidative stress induced by PN is associated with bronchopulmonary dysplasia, a main pathological complication in these babies who have weak antioxidant capacity to detoxify peroxides because of their...
Preprint
Most very premature newborns (< 32 weeks of gestation) receive parenteral nutrition (PN) that is inherently contaminated with peroxides. Oxidative stress induced by PN is associated with bronchopulmonary dysplasia, a main pathological complication in these babies who have weak antioxidant capacity to detoxify peroxides because of their glutathione...
Article
Extremely premature birth is associated with a permanent disruption of energy metabolism. The underlying mechanisms are poorly understood. The oxidative stress induced by parenteral nutrition (PN) during the first week of life is suspected to reprogram energy metabolism in the liver. Full-term male Hartley guinea pigs (to isolate PN from prematurit...
Article
Full-text available
Peroxides contaminating parenteral nutrition (PN) limit the use of methionine as a precursor of cysteine. Thus, PN causes a cysteine deficiency, characterized by low levels of glutathione, the main molecule used in peroxide detoxification, and limited growth in individuals receiving long-term PN compared to the average population. We hypothesize th...
Article
Full-text available
Background Parenteral nutrition (PN) is necessary for preterm infants unable to receive enteral nutrition. However, PN is contaminated by peroxides inducing oxidative stress, which is known to modify the enzymes involved in DNA methylation. Therefore, alteration of DNA methylation is suspected in infants on PN. Among PN ingredients, lipid emulsion...
Article
Full-text available
Background: Hypothermia is increasingly tested in several neurological conditions, such as neonatal encephalopathy, stroke, traumatic brain injury, subarachnoid hemorrhage, spinal cord injury, and neurological outcomes of cardiac arrest. Current studies aim to increase benefits of hypothermia with new add-on therapies including immunomodulatory ag...
Article
Full-text available
Based on the review of the literature, perinatal inflammation often induced by infection is the only consistent independent risk factor of neonatal arterial ischemic stroke (NAIS). Preclinical studies show that acute inflammatory processes take place in placenta, cerebral arterial wall of NAIS-susceptible arteries and neonatal brain. A top research...
Article
Full-text available
The medium-size intra-cranial arteries arising from the carotid bifurcation are prone to perinatal arterial ischemic strokes (PAIS). PAIS' physiopathology needs to be better understood to develop preventive and therapeutic interventions that are currently missing. We hypothesized that materno-fetal inflammation leads to a vasculitis affecting selec...
Article
Background: Despite the recent introduction of hypothermia as a mandatory standard of care, the incidence of neonatal encephalopathy in full-term newborns and its devastating neuro-behavioral outcomes continues to be a major individual, familial and social issue. Neonatal encephalopathy is mainly due to the compounding and interacting effects of h...
Article
Infection or inflammation during pregnancy is known to lead to maternal immune activation triggering a fetal inflammatory response syndrome associated with deleterious effects, such as brain injury and neurodevelopmental disabilities. Group B Streptococcus (GBS) - one of the most common bacterium colonizing pregnant women - can be responsible for c...
Poster
Full-text available
Poster presented at the International Society for Developmental Neuroscience (ISDN) meeting, May 11-14, 2016.
Chapter
It is known that gestational and/or perinatal inflammation combined or not with hypoxia-ischemia (HI) is a risk factor for brain injuries, but the mechanisms underlying are still unclear. This chapter discusses about animal models mimicking those conditions, allowing scientists to uncover mechanisms involved and to study the adverse effects on the...
Article
Full-text available
Inflammation due to remote pathogen exposure combined to hypoxia/ischemia (HI) is one of the most common causes of neonatal encephalopathy affecting at-term or near-term human newborn, which will consequently develop cerebral palsy. Within term-equivalent rat brains exposed to systemic lipopolysaccharide (LPS) plus HI, it was previously showed that...
Conference Paper
Introduction: Perinatal arterial ischemic strokes affect about 1/3,000 newborn and are the main cause of hemiplegic cerebral palsy. The large cerebral arteries from the anterior system, namely the intra-cranial carotid bifurcation, are the most affected, ischemic stroke being located in its territory in 85% of cases. The classic, but unproven, path...
Article
Introduction: Perinatal arterial ischemic strokes affect about 1/3,000 newborn and are the main cause of hemiplegic cerebral palsy. The large cerebral arteries from the anterior system, namely the intra-cranial carotid bifurcation, are the most affected, ischemic stroke being located in its territory in 85% of cases. The classic, but unproven, path...

Questions

Question (1)
Question
I performed Red Oil staining to stain lipids. I had a lot of background at the beginning with red oil drops "on top" of the plan of the cells. I changed a few steps to remove as much background staining as possible but I am not sure if my staining is properly done and if I can use it to analyse important tissues. Could you give me your opinion about the staining and tell me if you think it is ready to analyse ?
Many thanks !!

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