Cleide M F Soares

• Doctor Chemical Enginnier
• Universidade Tiradentes

Bioactive compounds are important for promoting human health, either by developing functional products that offer complementary health benefits or by incorporating them into nutritional supplements, beverages, fortified foods, and pharmaceutical products. In this context, this work focuses on extracting phenolic compounds from the mesocarp of licur...

This study describes the production of solketal esters from used soybean cooking oil (USCO) via enzymatic hydroesterification. This process consists of the complete hydrolysis of USCO into free fatty acids (FFAs) catalyzed by crude lipase extract from Candida rugosa (CRL). The resulting FFAs were recovered and utilized as the raw material for an es...

This work studies the partition of phenolic compounds, namely caffeic acid, syringic acid, vanillic acid, ferulic acid, and vanillin, in aqueous two-phase systems (ATPSs) formed by acetonitrile and deep eutectic solvents (DESs) based on choline chloride ([Ch]Cl) and carbohydrates (sucrose, d-glucose, d-mannose, arabinose, and d-xylose). The binodal...

Molecular docking is an important computational analysis widely used to predict the interaction of enzymes with several starting materials for developing new valuable products from several starting materials, including oils and fats. In the present study, molecular docking was used as an efficient in silico screening tool to select biocatalysts wit...

BACKGROUND Humic acids (HAs) are macromolecules classified within the group of humic substances that are mainly related to the agricultural and environmental sectors, but they have attracted interest from the medicine, pharmaceutical and cosmetic industries. However, these latter sectors depend on developing extraction and purification technologies...

This work presents a framework for evaluating hybrid nanoflowers using Burkholderia cepacia lipase. It was expanded on previous findings by testing lipase hybrid nanoflowers (hNF-lipase) formation over a wide range of pH values (5–9) and buffer concentrations (10–100 mM). The free enzyme activity was compared with that of hNF-lipase. The analysis,...

Estrogens are emergent pollutants found in low levels in water bodies, and for this reason they require concentration for quantification. In this study, aqueous two‐phase systems (ATPSs) were used as an alternative strategy to improve hormone detection and quantification from real water samples. In addition, a predictive computational method (COSMO...

BACKGROUND The present work provides diagrams for water‐free two‐phase systems based on polypropylene glycol 2000 (PPG 2000) + [C2mim][OAc] + alcohols (primary: methanol, ethanol, 1‐propanol and 1‐butanol; secondary: 2‐propanol and 2‐butanol; and tertiary: 2‐methyl‐2‐propanol) at 298.15 ± 1.00 K and 0.10 ± 0.01 MPa. As a proof of concept, the syste...

Biosurfactants are surface-active molecules, produced by several microorganisms, that possess unique properties such as low toxicity and biodegradability. Their application in various industries depends on their purity and their specific properties, such as emulsification and stability. Therefore, this study focuses on the production of biosurfacta...

001] A presente patente de invenção (PI) diz respeito a um processo para concentração de agrotóxicos com o intuito de se obter um produto com potencial biotecnológico, visando a identificação e quantificação de contaminantes em águas de abastecimento humano ou efluentes. Como característica diferencial do dito processo e produto obtido proposto nes...

CAMPO DA INVENÇÃO [001] A presente patente de invenção refere-se a um novo sistema etanolicos bifásicos (SEBs) para obtenção e purificação de biomoléculas de baixa ou nula solubilidade em água, mais especificamente, um novo sistema etanolicos bifásicos (SEBs) para obtenção de biomoléculas presentes no urucum, preferencialmente a bixina, e, diante d...

This work investigated the effect of different extracting agents on the extraction of phenolic compounds from agro-industrial residues of seriguela. Additionally, the phenolic compounds partitioning was studied using aqueous two-phase systems based on the extracting agents. The effect of the type of extracting agent such as water, organic solvent (...

Human milk fat (HMF) triacylglycerols (TAGs) mainly contain palmitic acid esterified at the sn-2 position while oleic and other unsaturated fatty acids are located at positions sn-1,3. This study aimed at the production of HMF substitutes (HMFS) by lipase-catalyzed acidolysis of tripalmitin with oleic acid, in a solvent-free medium. Burkholderia ce...

Crude olive pomace oil (OPO) is a by-product of olive oil extraction. In this study, low-calorie structured triacylglycerols (TAGs) were produced by acidolysis of crude OPO with medium-chain fatty acids (caprylic, C8:0; capric, C10:0) or interesterification with their ethyl ester forms (C8EE, C10EE). These new TAGs present long-chain fatty acids (L...

In this study, a comparative evaluation was performed in batch esterification reactions under conventional heating (CH) and assisted by microwave irradiation (MW) using bioprinted lipases. Microwave-irradiation-assisted reactions generally provide higher productivities and improve synthesis performance in terms of increased rate and reduced reactio...

The present research aims to study the process of immobilization of lipase from Burkholderia cepacia by physical adsorption on graphene oxide derived (GO) from grape seed biochar. Additionally, the modified Hummers method was used to obtain the graphene oxide. Moreover, Fourier transform infrared spectroscopy, Raman spectrum, X-ray diffraction, and...

In recent years, fluctuating global fossil fuel market prices and growing concern about environmental pollution have increased efforts to obtain novel value-added products from renewable agricultural biomass. To this end, a wide variety of triacylglycerols (edible and non-edible oils and fats) and their derivatives (free fatty acids or monoalkyl es...

The enzymatic production of isoamyl levulinate via esterification of isoamyl alcohol (IA) and levulinic acid (LA), a biomass-based platform chemical with attractive properties, in a solvent system has been performed in this study. For such a purpose, a low-cost liquid lipase (Eversa® Transform 2.0) immobilized by physical adsorption via hydrophobic...

Enzymes are promising tools for achieving an environmentally benign process. However, enzymes are required to be immobilized on economically competent supports to be reusable and maintain their activity. In this work, the aim was to evaluate the application of the biochar for immobilization of Burkholderia cepacia lipase (BCL) by physical adsorptio...

Cancer remains the cause of millions of deaths every year worldwide, and reliable early-stage diagnosis methods are essential to prevent them. Cancer biomarkers quantification in human fluids has gained relevance in detecting cancer in an early-stage. Among these, the levels of the biomarkers epinephrine, norepinephrine and vanillylmandelic acid in...

The industrial limitations for the use of enzymes are mainly related to their catalytic capacity and their high cost. Herein, it is proposed to evaluate the productive capacity of Burkholderia cepacia lipase (free enzyme and cross‐linked enzyme aggregates – CLEAs) for the hydrolysis and esterification reactions of licuri (Syagrus coronata (Mart.) B...

Lipases (E.C. 3.1.1.3) have buried active sites and used access tunnels in the transport of substrates and products for biotransformation processes. Computational methods are used to predict the trajectory and energy profile of ligands through these tunnels, and they complement the experimental methodologies because they filter data, optimizing lab...

Promising and sustainable alternatives for hydrogen production have been investigated. Among these, the use of enzymes may represent an efficient alternative. In this work, an increase in hydrogen production under simulated sunlight by combining TiO2 and TiO2/MgCl2 with Burkholderia cepacia lipase (BCL) was reported. The samples (TiO2, TiO2/MgCl2,...

BACKGROUND Cetyl palmitate is a wax ester naturally found in sperm whale spermaceti, and it is of importance in the cosmetics industry. International regulations prohibit the sale of sperm whale spermaceti, so it is now being replaced by pure cetyl palmitate obtained by a chemical route or from mixtures based on jojoba oil. Another alternative is t...

Tomatoes (Solanum lycopersicum) represent the second most produced and consumed vegetable crop in the world and can be considered a source of various nutrients, such as minerals, fibre, phenolic compounds, and vitamins A (precursors: β-carotene) and E (α -tocopherol). The actions of vitamins in the regulation of biological functions and disease pre...

Motivation: α-Tocopherol is a molecule obtained primarily from plant sources that are important for the pharmaceutical and cosmetics industry. However, this component has some limitations such as sensitivity to oxygen, presence of light, and high temperatures. For this molecule to become more widely used, it is important to carry out a structural m...

Deep eutectic solvents (DES) are versatile, green, new generation solvents that can be used in liquid-liquid separation techniques such as aqueous two-phase systems (ATPS). These systems have been proposed as powerful platforms for the separation and purification of high-value biomolecules, however their industrial application requires further feas...

Os biolubrificantes são alternativas ecologicamente corretas aos óleos lubrificantes minerais, especialmente por serem biodegradáveis. Com base nisso, pesquisadores e empresários têm direcionado os seus esforços para desenvolver e implementar processos eficientes e sustentáveis sem onerar o financeiro das indústrias de lubrificantes. Para esse fim,...

The effect of abiotic factors, such as weather and climate can alter the properties of vegetable oils. In this work, the effects of the refining process and pre-drying of Moringa oleifera Lam. seeds collected in the dry and rainy seasons (seasonality) were evaluated on the characteristics of the extracted oils. The refined and crude dry season oils...

Olive pomace oil (OPO), a by-product of olive oil industry, is directly consumed after refining. The novelty of this study consists of the direct use of crude high acidic OPO (3.4–20% acidity) to produce added-value compounds, using sn-1,3-regioselective lipases: (i) low-calorie dietetic structured lipids (SL) containing caprylic (C8:0) or capric (...

The valorization of coffee processing waste by producing products with high added value is a promising approach toward a circular bioeconomy. Crude coffee silverskin oil can be directly used in enzymatic hydrolysis catalyzed by non-specific lipases or sn-1,3 regioselective lipases. Converting coffee silverskin oil into free fatty acids or enriched...

In the present study, we demonstrated the use of molecular docking as an efficient in silico screening tool for lipase–triglyceride interactions. Computational simulations using the crystal structures from Burkholderia cepacia lipase (BCL), Thermomyces lanuginosus lipase (TLL), and pancreatic porcine lipase (PPL) were performed to elucidate the cat...

Here, we present a biorefinery perspective for biolubricant production with the valorisation of solid waste from Moringa oleifera Lam oil extraction (WMO). To achieve this, WMO, silica (SiO2), and a hybrid (SiO2–WMO) were prepared and employed as supports for the immobilisation of Candida rugosa lipase (CRL) via interfacial adsorption. The matrixes...

This work studied the phase separation in systems formed by polypropylene glycol (PPG) with different molecular weights (425, 725, 1000, 2000 g mol⁻¹) + ethylene glycol + ethanol, PPG + imidazolium chloride-based ionic liquid (IL) + ethanol, and PPG + ethylene glycol + IL (as adjuvant) + ethanol, which are namely Ethanolic Two-Phase System (ETPS)....

Abstarct Antidepressants are drugs widely used in nervous disorders, such as anxiety, depression, and panic, and are eliminated through the urinary system. In bodies of water, they present high persistence and toxicity, even at low concentrations, conforming to the classification of emerging micropollutants. This work addresses the construction of...

Este trabalho discute como a inovação tecnológica e a pesquisa científica podem levar ao desenvolvimento de novos produtos dentro de uma mesma linha industrial. Uma linha tradicional de processamento de leite pode produzir vários produtos lácteos, mas também pode ser aprimorada para produzir compostos de alta tecnologia com alto valor econômico agr...

BACKGROUND: The objective of this work was the development of silver nanoparticles (AgNPs) coated with humic acid (HA) and their incorporation into an enamel for antifungal activity. AgNPs were synthesized by chemical reduction using sodium boro-hydride as a reducing agent and coated with HA. Uncoated AgNPs were synthesized as control. AgNPs and HA...

Environmental contamination by atrazine leads to serious environmental impact. The low concentrations of atrazine in aqueous media render its identification and quantification difficult. In this context, aqueous two‐phase systems based on tetrahydrofuran (THF) + glycerol were tested as simultaneous recovery and preconcentration platforms of atrazin...

This study investigated the coffee silverskin (CS) crude oil extraction process and characterization of physicochemical properties and enzymatic hydrolysis for fatty acids production. The soxhlet and ultrasonic extractions showed CS oil yield similar to 3.8% and 3.1%, respectively. CS oil extracted by soxhlet presented favorable physicochemical pro...

BACKGROUND The objective of this work was the development of silver nanoparticles (AgNPs) coated with humic acid (HA) and their incorporation into an enamel for antifungal activity. AgNPs were synthesized by chemical reduction using sodium borohydride as a reducing agent and coated with HA. Uncoated AgNPs were synthesized as control. AgNPs and HA‐A...

The downstream processing still represents the cost bottleneck of obtaining biomolecules. Aqueous two-phase systems (ATPS) composed of alternative constituents have been presented as a cost-effective platform for the concentration, partition and/or purification of biomolecules. In this sense, this work provides a series of new ATPS composed of poly...

A presente patente de invenção (PI) diz respeito a um processo de aplicação dos hidrolisados proteicos oriundos da beta vignina do feijão caupi com ação biológica, no intuito de se obter um produto com potencial farmacêutico, alimentício e biotecnológico para atividade antimicrobiana. Os peptídeos antimicrobianos têm atividades biológicas testadas...

Oil from coffee silverskin (CS) is a potential source of fatty acids with promising applications in several industries. Thus, CS crude oil extraction processes were investigated for further enzymatic hydrolysis for fatty acids production. Firstly, Soxhlet (with 150 mL hexane for 8 hours at 70 °C) and ultrasound‐assisted (three times in sequential w...

This work reports, for the first time, an Ethanolic Two-Phase System (ETPS) based on polypropylene glycol 2000 (PPG 2000), mono-, di-, tri-ethylene glycol, and ethanol. An ionic liquid (IL) (1-butyl-3-methylpyridinium chloride) was used as an adjuvant. This ETPS shows promising results for the extraction of highly hydrophobic compounds. Bixin (mode...

A new design of cross-linked enzyme aggregates (CLEAs) of Burkholderia cepacia lipase (BCL) based mainly on the use of lignocellulosic residue of palm fiber as an additive was proposed. Different parameters for the preparation of active CLEAs in the hydrolysis of olive oil, such as precipitation agents, crosslinking agent concentration, additives,...

The use of microorganisms capable of mediating the bioprecipitation process can be an important application in the self-healing processes of cement specimens. Thus, the present study identified and evaluated five Bacillus strains for potential application in the protocol of self-healing via bioprecipitation. Cell growth, enzyme production, and kine...

This study aimed to evaluate the centesimal composition of Moringa oleifera Lam seeds and identify the physicochemical properties of crude oil extracted from Moringa oleifera Lam seeds. The values found for the centesimal composition showed that the seeds are constituted by 41% of crude oil, 32.40% of proteins, 4.20% of ash, 19.54% of carbohydrate,...

A study of biosensors formed with the enzyme horseradish peroxidase immobilized onto gold electrodes with cyclic voltammetry is presented here. This enzyme was chemically linked to self-assembled monolayers of 3-mercaptopropionic, 8-mercaptooctanoic and 11-mercaptoundecanoic acids. Gold electrodes were used as holders. The goal of this work was the...

The present work aims to evaluate the synergistic effect of imidazolium-based ionic liquids (IL) in the preparation and immobilization of Burkholderia cepacia lipase in a new hybrid support of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and silica. In addition, these biocatalysts were applied in transesterification reactions of crude coconut oi...

The aim of this study was the valorization of coffee industry residues, namely spent coffee grounds (SCG) as a source of oil, and silverskin (CS) as a source of both oil and biomass, under the concept of the circular economy. Therefore, crude oil from SCG was used to produce low-calorie structured lipids (SL) for food and pharmaceutical industries,...

Caatinga is a Brazilian semi-arid ecosystem that stands out for presenting unique environmental characteristics with a dry, spiny and deciduous shrub/forest vegetation with several species that can be renewable oil sources with potential applicability in oleochemical and nutrition. Caatinga oilseeds have a high content of unsaturated fatty acids, p...

Bioimprinting is an easy, sustainable and low‐cost technique that promotes a printing of potential substrates on enzyme structure, inducing a more selective and stable conformation. Bioimprinting promotes conformational changes in enzymes, resulting in better catalytic performance. In this work, the effect of bioimprinting of Burkholderia cepacia l...

In the present work the radish (Raphanus sativus L.) was used as the low-cost alternative source of peroxidase. The enzyme was immobilized in different supports: coconut fiber (CF), calcium alginate microspheres (CAMs) and silica SBA-15/albumin hybrid (HB). Physical adsorption (PA) and covalent binding (CB) as immobilization techniques were evaluat...

Guava seed biochar appears as a new alternative of effective support to the immobilization of Burkholderia cepacia lipase (BCL) by physical adsorption. The objective of this work was to evaluate the potential of this immobilized biocatalyst in the transesterification reaction of crude coconut oil and ethanol and to understand the mechanism of the r...

Brazilian red propolis has been proposed as a new source of compounds with cytotoxic activity. Red propolis is a resinous material of vegetal origin, synthesized from the bees of the Appis mellifera family, with recognized biological properties. To obtain actives of low polarity and high cytotoxic profile from red propolis, in this work, we propose...

In this study, crude oils extracted from spent coffee grounds (SCG) and olive pomace (OP) were used as raw-material to synthesize low-calorie triacylglycerols, either by acidolysis with capric acid, or by interesterification with ethyl caprate, in solvent-free media, catalyzed by sn-1,3 regioselective lipases. The Rhizopus oryzae lipase (ROL) was i...

BACKGROUND Rosemary leaves have been widely employed in the food and pharmaceutical industry due to the presence of important compounds. However, during the extraction process, in addition to the target biomolecules [chlorophyll (Chl), and rosmarinic acid], other components (phenolic compounds) are also extracted. This work focuses on the developme...

Aqueous two phase-systems (ATPS) have been introduced as a new approach to remove dyes from aqueous discharges. Thus, this work evaluated the phase formation capacity in ATPS based on different organic solvents (1,3 dioxolane and 2-propanol) and cholinium-based salts (cholinium bitartrate - [Ch][Bit] and cholinium dihydrogencitrate - [Ch][DHCit])....

Here, we have assessed the use one packed bed or two packed bed reactors in series in which Burkholderia cepacia lipase (BCL) was immobilised on protic ionic liquid (PIL)‐modified silica and used as a biocatalyst for the transesterification of crude coconut oil. Reaction parameters including volumetric flow, temperature and molar ratio were evaluat...

Estrogens are currently considered the most prescribed drugs in the world. These contaminants are not entirely metabolized by humans and therefore, they have been detected in different effluents. Several studies already demonstrated that the hormones are classified as the major responsible for causing endocrine changes in living organisms. In this...

In this work, a “green” Aspergillus niger lipase obtained from the solid-state fermentation of Hancornia speciosa (“mangaba”) seeds was efficiently immobilised on polyethersulfone membranes (PES) by physical adsorption (PES-ADS-lipase) and covalent bonding (PES-COV-lipase) (immobilisation yields of 92 and 81%, respectively). The free lipase showed...

Os Sistemas Aquosos Bifásicos (SABs) são compostos por duas fases aquosas imiscíveis que coexistem em equilíbrio termodinâmico, promovido pela adição de compostos solúveis em água. As composições dos SABs são representadas por diagramas de fases que expressam a concentração dos componentes do sistema. Cada SAB apresenta uma curva de equilíbrio que...

In this study, ascorbic acid and vanillin were recovered using semi-continuous and continuous operation of an aqueous two-phase system (ATPS) formed by ethanol (C2H5OH) and dipotassium hydrogen phosphate (K2HPO4). For operation, a column reactor was designed, allowing temperature control by a coating system and the circulation of up to two phases s...

The aim of this study was to evaluate the formation of aqueous two-phase systems (ATPS) based on different protic ionic liquids (PIL), synthesized using amines, and organic or inorganic acid, and acetonitrile (ACN). The ATPSs are used to pre-purify genipin from genipap (Genipa americana L.). All PILs (purity above 98%) were able to form ATPSs with...

O aumento na produção de resíduos sólidos motivou a criação de novas tecnologias para a disposição de lixo e esgoto. Os resíduos orgânicos representam 50% de toda a produção de resíduos sólidos nas cidades brasileiras, no entanto, a maior parte é descartada incorretamente. Uma solução para esse problema é o processo de compostagem, que converte res...

Biocatalysis has significant advantages over chemocatalysis in the context of sustainability since environmentally compatible catalysts (enzymes) and mild reaction conditions are used. However, enzymes are labile macromolecules, and strategies such as the addition of co-solvents or additives or their immobilization in solid supports are target of i...

Alternative strategies are required to develop the optimized production of fatty acids using biocatalysis; molecular docking and response surface methodology are efficient tools to achieve this goal. In the present study, we demonstrate a novel and robust methodology for the sustainable production of fatty acids from Moringa oleifera Lam oil using...

In this work, the effect of several phosphonium‐based ionic liquids (ILs) on the activity of lipase from Burkholderia cepacia (BCL) was evaluated by experimental assays and molecular docking. ILs comprising different cations ([P4444]⁺, [P444(14)]⁺, [P666(14)]⁺) and anions (Cl⁻, Br⁻, [Deca]⁻, [Phosp]⁻, [NTf2]⁻) were investigated to appraise the indi...

Aqueous two-phase systems (ATPS) have been proposed as platforms for the purification of biomolecules, and the application of adjuvants to tune the properties of ATPS phases and their ability to extract and separate biological products investigated. This work addresses, for the first time, the use of acetonitrile (ACN) as adjuvant in ATPS composed...

Diuron is a pesticide widely used in agriculture to control a variety of weeds, and therefore it is often detected in groundwater and surface water. However, the low levels of diuron in water make its identification and quantification unfeasible, causing great inconvenience in the evaluation of its persistence and environmental impact. In this cont...

Artificial neural network (ANN) techniques are effective in modeling nonlinear processes, are simple to implement and require low computational time. In this work, the lactose adsorption process for continuous flow in a fixed-bed column with a molecularly imprinted polymer (MIP) adsorbent was modeled using an ANN technique. The neural models allowe...

Liquid-liquid equilibrium data for systems based on acetonitrile + polyvinylpyrrolidone (PVP) (10000, 29000 and 40000 g·mol⁻¹) + water were experimentally measured at 298.15 ± 1.00 K and 0.1 ± 0.01 MPa. Merchuck's empirical equation was correlated with experimental binodal data, and the tie-lines and critical points (PCs) to complete the phase diag...

The increased demand for biotechnological processes in industrial applications requires the development of highly active and stable heterogeneous biocatalysts. For this reason, the present work aimed to study the immobilization process by physical adsorption of lipase from Burkholderia cepacia (BCL) on aerogel modified with protic ionic liquid (2-h...

O objetivo deste trabalho foi avaliar características bioquímicas da lipase de A. niger, obtida a partir da FES de resíduo de mangaba e imobilizada por adsorção física em membranas de poliétersulfona (PES). A lipase imobilizada apresentou atividade relativa acima de 70% em pHs alcalinos com máxima atividade no pH 11,0 e acima de 80% em temperaturas...