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Publications (84)
DNA metabarcoding provides a rapid and effective identification tool of macroinvertebrate species. The accuracy of species-level assignment, and consequent taxonomic coverage, relies on comprehensive DNA barcode reference libraries, which, due to incompleteness, are currently a recognized limitation for metabarcoding applications. In this study, we...
Coastal and freshwater cetaceans are particularly vulnerable due to their proximity to human activity, localized distributions and small home ranges. These species include Sotalia guianensis, found in the Atlantic and Caribbean coastal areas of central and South America, and Sotalia fluviatilis, distributed in the Amazon River and tributaries. We i...
In marine and estuarine benthic communities, the inventory and estimation of species richness are often hampered by the need for broad taxonomic expertise across several phyla. The use of DNA metabarcoding has emerged as a powerful tool for the fast assessment of species composition in a diversity of ecological communities. Here, we tested the ampl...
http://www.frontiersin.org/10.3389/conf.fmars.2016.05.00132/event_abstract
http://www.frontiersin.org/10.3389/conf.fmars.2016.05.00132/event_abstract
The Gastropoda is one of the best studied classes of marine invertebrates. Yet, most species have been
delimited based on morphology only. The application of DNA barcodes has shown to be greatly useful
to help delimiting species. Therefore, sequences of the cytochrome c oxidase I gene from 108 specimens
of 34 morpho-species were used to investigate...
Background: The Gastropoda are among the most diverse taxonomic groups of marine invertebrates and, together with other dominantclasses, such as Bivalvia, Malacostraca, and Polychaeta, constitute major components of marine benthic communities. As part of a wider effort to compile reference libraries for dominant benthic invertebrates of the NE Atla...
Background: The estuarine and coastal intertidal areas have a large number of benthic invertebrates, where the annelid polychaetes are one of the most representative classes and, therefore, important indicators of environmental quality in these ecosystems. Yet, these organisms have been poorly studied, in comparison to other taxa of similar ecologi...
Annelid polychaetes have been seldom the focus of dedicated DNA barcoding studies, despite their ecological relevance and often dominance, particularly in soft-bottom estuarine and coastal marine ecosystems. Here we report the first assessment of the performance of DNA barcodes in the discrimination of shallow water polychaete species from the sout...
The pink dolphin (Inia geoffrensis) is widely distributed along the Amazon and Orinoco basins, covering an area of approximately 7 million km2. Previous morphological and genetic studies have proposed the existence of at least two evolutionary significant units: one distributed across the Orinoco and Amazon basins and another confined to the Bolivi...
A large macrogeographic differentiation has been observed among Sotalia guianensis populations along the South American coast. However, no genetic structure has been detected so far in closely distributed
populations of this species, even though it has been observed in other cetaceans. Here, we examined the fine scale population
structure for the l...
The genus Chromobacterium encompasses free-living Gram-negative bacteria. Until 2007, the genus consisted of only one species but six species are now recognized. Chromobacterium violaceum is the type species of the genus and is commonly found in soil and water in tropical and sub-tropical regions. We have investigated a collection of 111 isolates d...
The Amazon River Dolphin (Inia geoffrensis) is widely distributed along the Amazon and Orinoco basins, covering an area of about 7 million km2. We have generated 519 base pair (bp) sequences of the control region (HVSI) and 1,140 bp of the Cytochrome B (Cyt-b) gene of mitochondrial DNA (mtDNA) for two populations from the Amazon basin in Brazil, se...
Incomplete and/or sluggish maltotriose fermentation causes both quality and economic problems in the ale-brewing industry.
Although it has been proposed previously that the sugar uptake must be responsible for these undesirable phenotypes, there
have been conflicting reports on whether all the known α-glucoside transporters in Saccharomyces cerevis...
In the current study we analyzed the kinetics of growth, sugar consumption, and ethanol production by a Saccharomyces cerevisiae strain with the AGT1 permease as its unique α-glucoside transporter on synthetic or rich medium containing maltose or maltotriose. Our results show that to efficiently consume and ferment these sugars from the medium requ...
Aims:
To enhance the fermentation of maltotriose by industrial Saccharomyces cerevisiae strains.
Methods and results:
The capability to ferment maltotriose by an industrial yeast strain that uses this sugar aerobically was tested in shake flasks containing rich medium. While the presence of maltose in the medium did not improve maltotriose ferme...
We studied the influence of cold temperature (10 degrees C) on the fermentation of maltose by a S. cerevisiae wild-type strain, and a csf1 Delta mutant impaired in glucose and leucine uptake at low temperatures. Cold temperature affected the fermentation kinetics by decreasing the growth rate and the final cell yield, with almost no ethanol been pr...
Fermentation of alpha-glucosides (maltose, maltotriose) by Saccharomyces cerevisiae cells is a critical phase in the processes of brewing and breadmaking. Utilization of alpha-glucosides requires the active transport of the sugar across the cell membrane and, subsequently, its hydrolysis by cytoplasmic glucosidases. Although transport activities ar...
Maltotriose, the second most abundant sugar of brewer's wort, is not fermented but is respired by several industrial yeast strains. We have isolated a strain capable of growing on a medium containing maltotriose and the respiratory inhibitor, antimycin A. This strain produced equivalent amounts of ethanol from 20 g l(-1) glucose, maltose, or maltot...
Saccharomyces cerevisiae grew slower but reached higher cellular densities when grown on 20 g maltotriose l–1 than on the same concentration of glucose or maltose. Antimycin A (3 mg l–1) prevented growth on maltotriose, but not on glucose or maltose, indicating that it is not fermented but is degraded aerobically. This was confirmed by the absence...
Abstract of the panel presented at the SBBq annual meeting (see attachament).
